Tissue resident memory cell profiles, and uses thereof

ABSTRACT

This disclosure provides methods of treating cancer or eliciting an anti-tumor response in a subject by administering an effective amount of a population of T-cells that exhibits higher or lower than baseline expression of one or more genes. In other aspects, methods are provided to diagnose cancer and determine prognosis of cancer patients. Also provided are methods to identify the antigens or antigen receptors associated with the isolated and/or purified cell populations that elicit a more positive prognosis.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims priority under 35 U.S.C. § 119(e) to U.S. Provisional Application No. 62/647,588, filed Mar. 23, 2018, and U.S. Provisional Application No. 62/770,412, filed Nov. 21, 2018, the content of each which is hereby incorporated by reference in its entirety.

BACKGROUND

High numbers of tissue-resident memory T (TRM) cells are associated with better clinical outcomes in cancer patients. However, the molecular characteristics that drive their efficient immune response to tumors are poorly understood. Thus, a need exists in the art to identify, characterize and harness these potent cells for therapeutic interventions. This disclosure satisfies this need and provides related advantages as well.

SUMMARY OF THE DISCLOSURE

To address the above identified limitations in the art, this disclosure provides methods of treating cancer or eliciting an anti-tumor response in a subject in need thereof, the methods comprising, or consisting essentially of, or consisting of administering to the subject an effective amount of a population of T-cells that exhibits higher or lower than baseline expression of one or more select genes. In one aspect, this method comprises, or consists essentially of, or yet further consists of administering to the subject an effective amount of an active agent that induces higher or lower than baseline expression of one or more genes, or the one or more genes itself.

For the disclosed methods, in one aspect, the one or more genes are set forth in Table 1, Table 2, Table 3, Table 4, Table 5, or Table 7. In another aspect, the one or more genes are set forth in Table 1 and/or Table 2.

In other aspects, provided are one or more methods of diagnosing cancer, identifying a subject likely to benefit from or respond to cancer treatment, (including but not limited to immunotherapy (including anti-cancer or anti-tumor immunotherapy)), determining the effectiveness of cancer treatment, and/or determining a prognosis of a subject having cancer. The one or more methods comprise, or alternatively consist essentially of, or yet further consist of, detecting or measuring the population or amount of TRMs, or a sub-population of TRMs expressing high levels of one or more of, or all three TIM3, CXCL13 and CD39, in the subject or in a sample isolated from the subject. In certain embodiments, a higher amount of TRMs or higher amount of the sub-population of TRMs expressing high levels of TIM3, CXCL13 and CD39 in the subject or sample indicates that the subject is likely to benefit from or respond to cancer treatment, including immunotherapy (e.g., anti-cancer or anti-tumor immunotherapy), that the cancer treatment is effective in the subject, or that the subject is likely to proceed have a positive clinical response, e.g., longer overall survival, remission or longer time to tumor progression or lack of cancer recurrence. In certain embodiments, a lower amount of TRMs or lower amount of the sub-population of TRMs expressing high levels of one or more of or all three TIM3, CXCL13 and CD39 in the subject or sample indicates that the subject is less likely to benefit from or respond to cancer treatment, including immunotherapy (including anti-cancer or anti-tumor immunotherapy), that the cancer treatment is not as effective in the subject as other therapies, or that the subject has a poor prognosis with available therapies.

In certain aspects, the cells are T-cells, CD8+ T-cells, tumor-infiltrating lymphocytes (TILs), tissue-resident memory (Trm) cells. In certain other aspects, the T-cells and/or TRMs are CD19⁻CD20⁻CD14⁻CD56⁻CD4⁻CD45⁺CD3⁺CD8 cells. In certain aspects, the TRMs are TRMs expressing high levels of one or more of or all three of TIM3, CXCL13 and CD39.

This disclosure also provides the isolated or purified T-cell populations that are modified to exhibit higher or lower than baseline expression of one or more genes. In certain aspects, the T-cells are isolated and/or purified from a patient population using the markers provided herein, e.g., CD19⁻CD20⁻CD14⁻CD56⁻CD4⁻CD45⁺CD3⁺CD8 or modified expression of one or more of, or all three of TIM3, CXCL13 and CD39. In certain aspects, the isolated or purified T-cells including modified populations of same, are expanded to create homogeneous or heterogenous cell populations and/or combined with carriers, such as pharmaceutically acceptable carriers. In some aspect, the cell populations are administered to a subject in need thereof as an adoptive cell therapy. In certain aspects, T-cells are cells engineered or modified to reduce or eliminate expression and/or the function of one or more genes.

Also provided herein are methods to identify the antigens or antigen receptors associated with the isolated and/or purified cell populations disclosed herein. In some aspect, the receptors are T-cell receptors (TCRs). In particular embodiments, the TCRs comprise one or more of the sequences listed in Table 6. In certain embodiments, the identified antigens or antigen receptors are used to vaccinate or treat a subject against cancer, cancer progression or an immune response. In other aspects, the identified antigens or antigen receptors are used to engineer cells, for example a chimeric-antigen receptor T-cell (CAR-T cell). In still other aspects, the engineered CAR-T cell are used to provide immunotherapy to a subject in need thereof, such as for example, a human patient.

Also provided herein are methods to induce an immune response and treat conditions requiring selective immunotherapy, comprising, or consisting essentially of, or yet further consisting of, contacting a target cell with the cells or compositions as described herein. The contacting can be performed in vitro, or alternatively in vivo, thereby providing immunotherapy to a subject such as for example, a human patient.

In one aspect, the cancer or tumor is in head, neck, lung, lung, prostate, colon, pancreas, esophagus, liver, skin, kidney, adrenal gland, brain, or comprises a lymphoma, breast, endometrium, uterus, ovary, testes, lung, prostate, colon, pancreas, esophagus, liver, skin, kidney, adrenal gland, or brain. In other aspects, the cancer comprises a metastasis or recurring tumor, cancer or neoplasia. In certain aspects, the cancer comprises a non-small cell lung cancer (NSCLC) or head and neck squamous cell cancer (HNSCC).

Provided herein is a method of treating cancer and/or eliciting an anti-tumor response in a subject comprising, or consisting essentially of, or yet further consisting of administering to the subject an effective amount of a population of T-cells that exhibit higher than or lower than baseline expression of one or more genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7, or that express a T-cell receptor comprising at least one of the amino acid sequences set forth in Table 6. In one aspect, the method comprises, or consists essentially of, or yet further consists of administering to the subject an effective amount of an agent that induces higher than or lower than baseline expression of one or more genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 in T-cells, or a T-cell receptor comprising at least one of the amino acid sequences set forth in Table 6. In another aspect, the method comprises, or consists essentially of, or yet further consists of administering an effective amount of one or more an agent that induces or inhibits in T-cells activity of one or more proteins encoded by genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 to the subject or sample. The active agent can be an antibody, a small molecule, a protein, a peptide, a ligand mimetic or a nucleic acid. The one or more gene may be selected from the group of 4-1BB, PD-1, CD103 or TIM3. In one aspect, the baseline expression is normalized mean gene expression. In another aspect, the higher than baseline expression is at least about a 2-fold increase in expression relative to baseline expression and/or lower than baseline expression is at least about a 2-fold decrease in expression relative to baseline expression. In a further aspect, the T-cells are tissue-resident memory cells (T_(RM)) or CD8+ T-cells. In one particular embodiment, the T-cells are autologous to the subject being treated. The methods of treating cancer and/or eliciting an anti-tumor response disclosed herein may further comprise, or consist essentially of, or yet further consist of administering to the subject an effective amount of a cytoreductive therapy. The cytoreductive therapy can be one or more of chemotherapy, immunotherapy, or radiation therapy.

Also disclosed herein is a modified T-cell modified to exhibit higher than or lower than baseline expression of one or more genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7, or to express a T-cell receptor comprising, or consisting essentially of, or yet further consisting of at least one of the amino acid sequences set forth in Table 6. The one or more gene may be selected from the group of 4-1BB, PD-1, CD103 or TIM3. In one aspect, the baseline expression is normalized mean gene expression. In another aspect, the higher than baseline expression is at least about a 2-fold increase in expression relative to baseline expression and/or lower than baseline expression is at least about a 2-fold decrease in expression relative to baseline expression. In a further aspect, the T-cells are tissue-resident memory cells (T_(RM)) or CD8+ T-cells. In one particular embodiment, the T-cells are autologous to the subject being treated.

The modified T-cell can be genetically modified, optionally using recombinant methods and/or a gene editing technology such as TALENs or a CRISPR/Cas system. The modified T-cell disclosed herein can also be further modified to express a protein that binds to a cytokine, chemokine, lymphokine, or a receptor each thereof. In one aspect, the protein comprises, or consists essentially of, or yet further consists of an antibody or an antigen binding fragment thereof. In another aspect, the antibody is an IgG, IgA, IgM, IgE or IgD, or a subclass thereof. The antibody can also be an IgG selected from the group of IgG₁, IgG₂, IgG₃ or IgG₄. Furthermore, the antigen binding fragment can be selected from the group of a Fab, Fab′, F(ab′)2, Fv, Fd, single-chain Fvs (scFv), disulfide-linked Fvs (sdFv) or V_(L) or V_(H).

In one aspect, the modified T-cell of this disclosure comprises, or consists essentially of, or yet further consists of modification that includes a chimeric antigen receptor (CAR). In one embodiment, the chimeric antigen receptor (CAR) comprises, or consists essentially of, or yet further consists of: (a) an antigen binding domain; (b) a hinge domain; (c) a transmembrane domain; (d) and an intracellular domain. The CAR can further comprise, or consist essentially of, or yet further consist of one or more costimulatory signaling regions. Further modifications are contemplated and within the scope of this disclosure, e.g., as reviewed in Ajina and Maher, (2018) Mol. Cancer Ther. 17(9):1795-1815. In one embodiment, the antigen binding domain comprises, or consists essentially of, or yet further consists of an anti-CD19 antigen binding domain, the transmembrane domain comprises, or consists essentially of, or yet further consists of a AMICA1, a CD28H (TMIGD2), a CD28 or a CD8α transmembrane domain and the one or more costimulatory regions selected from a CD28 costimulatory signaling region, a 4-1BB costimulatory signaling region, an AMICA1 costimulatory signaling region, a CD28H (TMIGD2) costimulatory signaling region, an ICOS costimulatory signaling region, and an OX40 costimulatory region or a CD3 zeta signaling domain. In a further embodiment, the anti-CD19 binding domain comprises, or consists essentially of, or yet further consists of a single-chain variable fragment (scFv) that specifically recognizes a humanized anti-CD19 binding domain. The anti-CD19 binding domain scFv of the CAR may comprise, or consist essentially of, or yet further consist of a heavy chain variable region and a light chain variable region.

In one aspect, the anti-CD19 binding domain of the CAR further comprises, or consists essentially of, or yet further consists of a linker polypeptide located between the anti-CD19 binding domain scFv heavy chain variable region and the anti-CD19 binding domain scFv light chain variable region. The linker polypeptide of the CAR may comprise, or consist essentially of, or yet further consist of a polypeptide of the sequence (GGGGS)n wherein n is an integer from 1 to 6. In another aspect, the CAR can further comprise, or consist essentially of, or yet further consist of a detectable marker attached to the CAR. In a separate aspect, the CAR can further comprise, or consist essentially of, or yet further consist of a purification marker attached to the CAR.

Further provided herein is a modified T-cell comprising, or consisting essentially of, or yet further consisting of a polynucleotide encoding the CAR, and optionally, wherein the polynucleotide encodes and anti-CD19 binding domain. In one aspect, the polynucleotide may further comprise, or consist essentially of, or yet further consist of a promoter operatively linked to the polynucleotide to express the polynucleotide in the modified T-cell. In another aspect, the polynucleotide may further comprise, or consist essentially of, or yet further consist of a 2A self-cleaving peptide (T2A) encoding polynucleotide sequence located upstream of a polynucleotide encoding the anti-CD19 binding domain. In yet a further aspect, the polynucleotide may further comprise, or consist essentially of, or yet further consist of a polynucleotide encoding a signal peptide located upstream of a polynucleotide encoding the anti-CD19 binding domain. In one embodiment, the polynucleotide further comprises, or consists essentially of, or yet further consists of a vector. In one particular embodiment, the vector is a plasmid. In another embodiment, the vector is a viral vector selected from the group of a retroviral vector, a lentiviral vector, an adenoviral vector, and an adeno-associated viral vector.

Also disclosed herein is a composition comprising, or consisting essentially of, or yet further consisting of a population of modified T-cells described above. Further provided herein is a method of treating cancer in a subject and/or eliciting an anti-tumor response comprising, or consisting essentially of, or yet further consisting of administering to the subject or contacting the tumor with an effective amount of the modified T-cells disclosed herein and/or the composition of this disclosure.

Further provided herein is a method of diagnosing a subject for cancer, comprising, or consisting essentially of, or yet further consisting of contacting a sample isolated from the subject with an agent that detects the presence of one or more genes set forth in Table 1, Table 2, Table 3, Table 4, Table Sand/or Table 7, wherein the presence of the one or more genes at higher or lower than baseline expression levels is diagnostic of cancer. In one aspect, the method comprises, or consists essentially of, or yet further consists of contacting tissue-resident memory cells (TRMs) isolated from the subject with an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAG3, an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds CD28H (TMIGD2), and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of CD8⁺PD1⁺, CD8⁺TIM3⁺, CD8⁺LAG3⁺, CD8⁺AMICA1⁺, CD8⁺CD28H⁺, CD8⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺, CD8⁺PD1⁺LAG3⁺, CD8⁺PD1⁺AMICA1⁺, CD8⁺PD1⁺CD281-r CD8⁺PD1⁺CTLA4⁺′CD8⁺TIM3⁺LAG3⁺, CD8⁺TIM3⁺AMICA1⁺, CD8⁺TIM3⁺CD28H⁺, CD8⁺TIM3⁺CTLA4⁺, CD8⁺LAG3⁺CTLA4⁺, CD8⁺LAG3⁺AMICA1⁺, CD8⁺LAG3⁺CD28H⁺, CD8⁺PD1⁺TIM3⁺LAG3⁺, CD8+LAG3⁺PD1⁺AMICA1⁺, CD8⁺LAG3⁺PD1⁺CD28H⁺, CD8⁺PD1⁺LAG3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺ AMICA1⁺′, CD8⁺PD1⁺TIM3⁺CTLA4⁺CD28H⁺, or CD8⁺PD1⁺TIM3⁺CTLA4⁺AMICA⁺CD28H⁺′ TRMs, wherein a high frequency of one or more of these TRMs is diagnostic of cancer.

In another aspect, the method of diagnosing cancer in a subject comprises, or consists essentially of, or yet further consists of contacting tissue-resident memory cells (TRMs) isolated from the subject with an antibody or agent that recognizes and binds one or more proteins encoded by a gene set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 and, optionally, an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAGS, an antibody or agent that recognizes and binds S1PR1, an antibody or agent that recognizes and binds CD28H (TMIGD2), an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds KLF3, an antibody or agent that recognizes and binds S1PR5, an antibody or agent that recognizes and binds KLF2 and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of TRMs expressing these proteins, wherein a high frequency of TRMs expressing these proteins is diagnostic of cancer.

Additionally, disclosed herein is a method of determining the density of tissue-resident memory cells (TRMs) in a cancer, tumor, or sample isolated from the subject likely to contain these cells, the method comprising, or consisting essentially of, or yet further consisting of measuring expression of one or more gene selected from the group of 4-1BB, PD-1, CD103, AMICA1, CD28H or TIM3 or genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 in the cancer, tumor, or sample thereof, wherein higher or lower than baseline expression indicates higher density of TRMs in the cancer, tumor, or sample thereof.

Further provided herein is a method of determining prognosis of a subject having cancer comprising, or consisting essentially of, or yet further consisting of measuring the density of tissue-resident memory cells (T_(RM)) in the cancer or a sample isolated from the patient, wherein a high density of T_(RM) indicates a more positive prognosis, e.g., an increased probability and/or duration of survival. In one aspect, the method of prognosis of a subject having cancer comprises, or consists essentially of, or yet further consists of contacting tissue-resident memory cells (TRMs) isolated from the subject (e.g., of the cancer or a sample thereof) with an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAG3, an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds CD28H (TMIGD2), and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of CD8⁺PD1⁺, CD8⁺TIM3⁺, CD8⁺LAG3⁺, CD8⁺AMICA1⁺, CD8⁺CD28H⁺, CD8⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺, CD8⁺PD1⁺LAG3⁺, CD8⁺PD1⁺AMICA1⁺, CD8⁺PD1⁺CD28H⁺, CD8⁺PD1⁺CTLA4⁺′CD8⁺TIM3⁺LAG3⁺, CD8⁺TIM3⁺AMICA1⁺, CD8⁺TIM3⁺CD28H⁺, CD8⁺TIM3⁺CTLA4⁺, CD8⁺LAG3⁺CTLA4⁺, CD8⁺LAG3⁺AMICA1⁺, CD8⁺LAG3⁺CD28H⁺, CD8⁺PD1⁺TIM3⁺LAG3⁺, CD8⁺LAG3⁺PD1⁺AMICA1⁺, CD8⁺LAG3⁺PD1⁺CD28H⁺, CD8⁺PD1⁺LAG3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺AMICA1⁺′, CD8⁺PD1⁺TIM3⁺CTLA4⁺CD28H⁺′ or CD8⁺PD1⁺TIM3⁺CTLA4⁺AMICA⁺CD28H⁺′ TRMs, wherein a high frequency of one or more of these TRMs indicates a more positive prognosis, e.g., an increased probability and/or duration of survival. In another aspect, the method of prognosis of a subject having cancer comprises, or consists essentially of, or yet further consists of contacting tissue-resident memory cells (TRMs) of the cancer or a sample thereof with an antibody or agent that recognizes and binds one or more proteins encoded by a gene set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 and, optionally, an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAG3, an antibody or agent that recognizes and binds S1PR1, an antibody or agent that recognizes and binds CD28H (TMIGD2), an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds KLF3, an antibody or agent that recognizes and binds S1PR5, an antibody or agent that recognizes and binds KLF2 and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of TRMs expressing these proteins, wherein a high frequency of TRMs expressing these proteins indicates a more positive prognosis, e.g., an increased probability and/or duration of survival.

In yet a further aspect, the method of determining prognosis of a subject having cancer comprises, or consists essentially of, or yet further consists of contacting tissue-resident memory cells (TRMs) isolated from the subject, (e.g., of the cancer or a sample thereof) with an antibody or agent that recognizes and binds CD103 to determine the frequency of CD103+ TRMs or an antibody or agent that recognizes and binds a protein encoded by a gene set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 to determine the frequency of TRMs expressing the protein, wherein a high or low frequency of TRMs expressing the protein indicates a more positive prognosis, e.g., an increased probability and/or duration of survival. In a separate aspect, the method of determining prognosis of a subject having cancer comprises, or consists essentially of, or yet further consists of measuring the density of CD103 or proteins encoded by one or more gene set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 in the sample (e.g., cancer or a sample thereof), wherein a high or low density of proteins indicates a more positive prognosis, e.g., an increased probability and/or duration of survival.

Also described herein is a method of determining the responsiveness of a subject having cancer to immunotherapy comprising, or consisting essentially of, or yet further consisting of contacting tissue-resident memory cells (TRMs) isolated from the subject, (e.g., of the cancer or a sample thereof) with an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds CD28H (TMIGD2), an antibody or agent that recognizes and binds LAG3, and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of CD8⁺PD1⁺, CD8⁺TIM3⁺, CD8⁺LAG3⁺, CD8⁺AMICA1⁺, CD8⁺CD28H⁺, CD8⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺, CD8⁺PD1⁺LAG3⁺, CD8⁺PD1⁺AMICA1⁺, CD8⁺PD1⁺CD28H⁺, CD8⁺PD1⁺CTLA4⁺, CD8⁺TIM3⁺LAG3⁺, CD8⁺TIM3⁺AMICA1⁺, CD8⁺TIM3⁺CD28H⁺, CD8⁺TIM3⁺CTLA4⁺, CD8⁺LAG3⁺CTLA4⁺, CD8⁺LAG3⁺AMICA1⁺, CD8⁺LAG3⁺CD28H⁺, CD8⁺PD1⁺TIM3⁺LAG3⁺, CD8⁺LAG3⁺PD1⁺AMICA1⁺, CD8⁺LAG3⁺PD1⁺CD28H⁺, CD8⁺PD1⁺LAG3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺AMICA1⁺′, CD8⁺PD1⁺TIM3⁺CTLA4⁺CD28H⁺′ or CD8⁺PD1⁺TIM3⁺CTLA4⁺AMICA⁺CD28H⁺′ TRMs, wherein a high frequency of one or more of these TRMs indicates responsiveness to immunotherapy. In one aspect, the method of determining the responsiveness of a subject having cancer to immunotherapy comprises, or consists essentially of, or yet further consists of contacting tissue-resident memory cells (TRMs) isolated from the subject, (e.g., of the cancer or a sample thereof) with an antibody that recognizes and binds one or more proteins encoded by a gene set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 and, optionally, an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAG3, an antibody or agent that recognizes and binds CD28H (TMIGD2), an

antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds KLF3, an antibody or agent that recognizes and binds S1PR5, an antibody or agent that recognizes and binds S1PR1, an antibody or agent that recognizes and binds KLF2 and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of TRMs expressing these proteins, wherein a high frequency of TRMs expressing these proteins indicates responsiveness to immunotherapy. For any of the methods disclosed herein, the TRMs may comprise, or consist essentially of, or yet further consist of CD19⁻CD20⁻CD14⁻CD56⁻CD4⁻CD45⁺CD3⁺CD8⁺ T-cells.

Further disclosed are methods of identifying a subject that will or is likely to respond to a cancer therapy, comprising, or consisting essentially of, or yet further consisting of contacting the same with an agent that detects the presence of one or more genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 in a sample isolated from the subject, (e.g., the cancer or a sample thereof), wherein the presence of the one or more genes at higher or lower than baseline expression levels indicates that the subject is likely to respond to cancer therapy. In one aspect, the baseline expression is normalized mean gene expression. In another aspect, the higher than baseline expression is at least about a 2-fold increase in expression relative to baseline expression and/or lower than baseline expression is at least about a 2-fold decrease in expression relative to baseline expression. The method may further comprise, or consist essentially of, or yet further consist of administering a cancer therapy to the subject. The cancer therapy or cytoreductive therapy can be chemotherapy, immunotherapy, radiation therapy, and/or administering to the subject or contacting the tumor with an effective amount of the modified T-cells and/or the composition of this disclosure.

The cancer, tumor, or sample can be contacted with an agent, optionally including a detectable label or tag. In one aspect, the detectable label or tag can comprise, or consist essentially of, or yet further consist of a radioisotope, a metal, horseradish peroxidase, alkaline phosphatase, avidin or biotin. In another aspect, the agent can comprise, or consist essentially of, or yet further consist of a polypeptide that binds to an expression product encoded by the gene, or a polynucleotide that hybridizes to a nucleic acid sequence encoding all or a portion of the gene. The polypeptide may comprise, or consist essentially of, or yet further consist of an antibody, an antigen binding fragment thereof, or a receptor that binds to the gene. In one aspect, the antibody is an IgG, IgA, IgM, IgE or IgD, or a subclass thereof. In another aspect, the IgG antibody is an IgG₁, IgG₂, IgG₃ or IgG₄. The antigen binding fragment can be a Fab, Fab′, F(ab′)2, Fv, Fd, single-chain Fvs (scFv), disulfide-linked Fvs (sdFv) or V_(L) or V_(H). In one aspect, the agent is contacted with the cancer, tumor, or sample in conditions under which it can bind to the gene it targets.

The methods of this disclosure comprise, or consist essentially of, or yet further consist of detection by immunohistochemistry (IHC), in-situ hybridization (ISH), ELISA, immunoprecipitation, immunofluorescence, chemiluminescence, radioactivity, X-ray, nucleic acid hybridization, protein-protein interaction, immunoprecipitation, flow cytometry, Western blotting, polymerase chain reaction, DNA transcription, Northern blotting and/or Southern blotting. The sample may comprise, or consist essentially of, or yet further consist of cells, tissue, an organ biopsy, an epithelial tissue, a lung, respiratory or airway tissue or organ, a circulatory tissue or organ, a skin tissue, bone tissue, muscle tissue, head, neck, brain, skin, bone and/or blood sample. While the cancer or tumor described herein can be an epithelial, a head, neck, lung, lung, prostate, colon, pancreas, esophagus, liver, skin, kidney, adrenal gland, brain, or comprises a lymphoma, breast, endometrium, uterus, ovary, testes, lung, prostate, colon, pancreas, esophagus, liver, skin, kidney, adrenal gland and/or brain cancer or tumor, a metastasis or recurring tumor, cancer or neoplasia, a non-small cell lung cancer (NSCLC) and/or head and neck squamous cell cancer (HNSCC).

In a further aspect, the methods of this disclosure comprise, or consist essentially of, or yet further consist of, detecting in the subject, in the cells or in a sample isolated from the subject, the number or density of Trm cells that are CD19-CD20-CD14-CD56-CD4-CD45⁺CD3⁺CD8+ T-cells.

Finally, provided herein is a kit comprising, or consisting essentially of, or yet further consisting of one or more of the modified T-cells and/or the composition of this disclosure and instructions for use. In one aspect, the instruction for use provide directions to conduct any of the methods described herein.

BRIEF DESCRIPTION OF THE DRAWINGS

The drawings illustrate embodiments of the technology and are not limiting. For clarity and ease of illustration, the drawings are not made to scale, and, in some instances, various aspects may be shown exaggerated or enlarged to facilitate an understanding of particular embodiments.

FIGS. 1A-1F: CD103 expressing CTLs in human lungs are enriched for tissue residency features but are transcriptionally distinct from previously characterized TRM cells. (FIG. 1A) tSNE plot of lung TRM (CD103⁺) and non-T_(RM) (CD103⁻) CTLs. Each symbol represents an individual patient sample (n=21 non-T_(RM); n=20 T_(RM)). (FIG. 1B) RNA-Seq analysis of transcripts (one per row) expressed differentially between lung T_(RM) and lung non-T_(RM), (pairwise comparison; change in expression of 2-fold with an adjusted P value of <0.05 (DESeq2 analysis; Benjamini-Hochberg test)), presented as row-wise z-scores of transcripts per million (TPM). Each column represents an individual sample; key known TRM or non-T_(RM) transcripts are indicated. Color scheme and number of samples is identical to (FIG. 1A). (FIG. 1C) GSEA of the murine composite TRM signature in the transcriptome of lung TRM vs. lung non-T_(RM): top, running enrichment score (RES) for the gene set, from most over-represented genes at left to most under-represented at right; middle, positions of gene set members (blue vertical lines) in the ranked list of genes; bottom, value of the ranking metric. Values above the plot represent the normalized enrichment score (NES) and false discovery rate (FDR)-corrected significance value. (FIG. 1D) Flow-cytometry analysis of the expression of CD49A and KLRG1 versus that of CD103 among live and singlet-gated CD19⁻CD20⁻CD14⁻CD45⁺CD3⁺CD8⁺ cells obtained from lung; right, frequency of CD103+ CTLs or CD103⁻ CTLs that express the indicated surface marker (*P≤0.05, n=6), bars represent the mean, t-line the s.e.m., and symbol represents data from individual samples. (FIGS. 1E-1F) Venn diagrams (upper) showing overlap of transcripts differentially expressed in lung TRM versus other previously characterized TRM cells. Waterfall plots (lower) represent the DESeq2 normalized fold change of genes not significantly (<2-fold) differentially expressed between lung TRM (CD103⁺) and non-T_(RM) (CD103⁻) CTLs.

FIGS. 2A-2H: TRM cells in normal lung and lung tumors share tissue residency features but are otherwise distinct. (FIG. 2A) GSEA of murine composite TRM signature in the transcriptome of lung tumor TRM vs. that of tumor non-T_(RM) cells; top, running enrichment score (RES) for the gene set, from most over-represented genes at left to most under-represented at right; middle, position of gene set members (blue vertical lines) in the ranked list of genes; bottom, value of the ranking metric. Values above the plots represent the normalized enrichment score (NES) and FDR-corrected significance value. (FIG. 2B) tSNE plot of tumor and lung CTL transcriptomes segregated by CD103 expression (lung non-T_(RM)=21, lung TRM=20, tumor non-T_(RM)=25, tumor TRM=19). (FIG. 2C) Venn diagram and (FIG. 2D) heat map of RNA-Seq analysis of 89 common transcripts (one per row) expressed differentially by lung TRM versus lung non-T_(RM), and tumor TRM versus tumor non-T_(RM) (pairwise comparison; change in expression of 2-fold with an adjusted P value of <0.05 (DESeq2 analysis; Benjamini-Hochberg test)), presented as row-wise z-scores of TTPM; each column represents an individual sample; key known TRM or non-T_(RM) transcripts are indicated. Color scheme and number of samples is identical to (FIG. 2B). (FIG. 2E) Spearman co-expression analysis of the 89 differentially expressed genes as in (c) and (d); values are clustered with complete linkage. A topological overlap matrix was calculated at power 5 using weighted gene co-expression network analysis and visualized in Gephi. The nodes are colored and sized according to the number of edges (connections), and the edge thickness is proportional to the edge weight (strength of correlation). The network layout is assigned by the Fruchterman-Reingold algorithm, using Noverlap to prevent overlapping labels. (FIG. 2F) Quantitated expression according to RNA-Seq data of the indicated differentially expressed genes shared by lung and tumor TRM cells. Each symbol represents an individual sample, the bar represents the mean and colored as in (FIG. 2B), t-line the s.e.m. (FIG. 2G) Flow-cytometry analysis of the expression of PD1 versus that of CD103 on live and singlet-gated CD19⁻CD20⁻CD14⁻CD56⁻CD4⁻CD45⁺CD3⁺CD8⁺ cells obtained from lung cancer TILs; right, frequency of cell that express PD-1 in the indicated populations (* P≤0.05; n=8), each symbol represents a sample, bars represent the mean, t-line the s.e.m. (FIG. 2H) RNA-Seq analysis of genes (row) up- or downregulated in the 4 cell types following 4 h of ex vivo stimulation. Left, heat map as in (FIG. 2D); right, bar graphs showing expression of transcripts in the indicated populations (n=6 for all comparisons; represented as in (FIG. 2F)).

FIGS. 3A-3F: Tumor T_(RM) cells proliferate, express the inhibitory checkpoint TIM3 and markers of enhanced function. (FIG. 3A) RNA-Seq analysis of transcripts (one per row) differentially expressed by tumor TRM relative to lung T_(RM), lung non-T_(RM), and tumor non-T_(RM) (pairwise comparison; change in expression of 2-fold with an adjusted P value of <0.05 (DESeq2 analysis; Benjamini-Hochberg test)), presented as row-wise z-scores of TPM; each column represents an individual sample (lung non-T_(RM)=21, lung TRM=20, tumor non-T_(RM)=25, tumor TRM=19). (FIG. 3B) Summary of over-representation analysis (using Reactome) of genes involved in the cell cycle that are differentially expressed by lung tumor T_(RM) relative to the other lung CTLs; q values represent false discovery rate (FIG. 3C) Shannon-Wiener diversity and Inverse Simpson indices obtained using V(D)J tools following TCR-seq analysis of β chains in tumor TRM and tumor non-T_(RM) populations. Bars represent the mean, t-line the s.e.m., and symbols represent individual data points (**P<0.01; n=10 patients). (FIG. 3D) Left, bar graphs show the percentage of total TCRβ chains that were expanded (≥3 clonotypes). Bars represent the mean, t-line the s.e.m., and dots individual data points (** P≤0.01; n=10 patients). Right, pie charts show the distribution of TCRβ clonotypes based on clonal frequency. (FIG. 3E) Left, Spearman co-expression analysis of the 77 genes up-regulated (FIG. 3A) in tumor TRM cells; values are clustered with complete linkage. Right, topological overlap matrix calculated at power 5 using weighted gene co-expression network analysis and visualized in Gephi. Node color and size are scaled according to the number of edges, edge thickness is proportional to the weight, and the network layout is assigned by the Fruchterman-Reingold algorithm, using Noverlap to prevent overlapping labels. (FIG. 3F) Correlation of the expression of HAVCR2 (TIM3) transcripts and the indicated transcripts in tumor TRM population; r indicates Spearman correlation value (*P≤0.05; *** P≤0.001; **** P≤0.0001).

FIGS. 4A-4G: Single-cell transcriptomic analysis reveals previously uncharacterized TRM subsets. (FIG. 4A) tSNE visualization of ˜12,000 live and singlet-gated, CD19⁻CD20⁻CD14⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples. Each symbol represents a cell; color indicates protein expression of CD103 detected by flow cytometry. (FIG. 4B) Seurat clustering of cells in (FIG. 4A) identifying 9 clusters. (FIG. 4C) Cells from tumor and lung were randomly downsampled to equivalent numbers of cells. Left, distribution of T_(RM)-enriched clusters in tumor and lung. Right, pie chart representing the relative proportions of cells in each TRM cluster. (FIG. 4D) Expression of transcripts previously identified as upregulated in the bulk tumor TRM population (FIG. 3A) by each cluster; each column represents the average expression in a particular cluster. (FIG. 4E) Breakdown of cell type and tissue localization of cells defined as being in cluster 1. (FIG. 4F) Violin plots of expression of example tumor TRM genes in each T_(RM)-enriched cluster (square below indicates the cluster type); shape represents the distribution of expression among cells and color represents the Seurat-normalized average expression. (FIG. 4G) Cell-state hierarchy maps generated by Monocle2 bioinformatics modeling of the TRM clusters; center plot, each dot represents a cell colored according to Seurat-assigned assigned cluster; surrounding panels show relative Seurat-normalized expression of the indicated genes.

FIGS. 5A-5D: A subset of tumor TRM cells has a transcriptional program indicative of superior functional properties. (FIG. 5A) Single-cell RNA-Seq analysis of transcripts (one per row) uniquely differentially expressed by each tumor TRM subset in pairwise analysis compared to other clusters (adjusted P value of <0.01; MAST analysis), presented as row-wise z-scores of Seurat-normalized count, each column represents an individual cell. Horizontal breaks separate genes enriched in each of the 4 tumor TRM subtypes. (FIG. 5B) Seurat-normalized expression of indicated transcripts identified as differentially enriched in each cluster, overlaid across the tSNE plot, with expression levels represented by the color scale. (FIG. 5C) Violin plot of expression of functionally important genes identified as significantly enriched in the ‘highly functional’ TRM subset; shape represents the distribution of expression among cells and color represents the Seurat-normalized average expression. The 91 transcripts enriched in cluster 2 compared to the other TRM clusters included several which encoded products linked to cytotoxic activity such as PRF1, GZMB, GZMA, CTSW³⁸, and CRTAM³⁸, as well as transcripts encoding effector cytokines and chemokines such as IFNγ, CCL3, CXCL13, IL17A and IL26. TRM cells exhibited a transcriptional program suggestive of superior effector properties and cell proliferation expressed high transcript levels for cytotoxicity molecules (Perforin, Granzyme A and Granzyme B) and several co-stimulatory molecules such as 4-1BB, ICOS and GITR (TNFRSF18). (FIG. 5D) Top, violin plot of expression of genes encoding key effector molecules in specific tumor-infiltrating CTL subsets. Below, percentage of cells expressing IFNG transcripts in each population, where positive expression was defined as greater than 1 Seurat-normalized count; “Other T_(RM)” corresponds to tumor CTLs isolated from clusters 3, 4, and 5.

FIGS. 6A-6J: PD-1- and TIM3-expressing tumor-infiltrating TRM lack an exhausted phenotype and exhibit enhanced clonal expansion. (FIG. 6A) GSEA of ‘highly functional’ TRM signature in the transcriptome of clonally expanded tumor TRM vs. that of non-expanded TRM cells: top, running enrichment score (RES) for the gene set, from most over-represented at left to most under-represented at right; middle, positions of gene set members (blue vertical lines) in the ranked list of genes; bottom, value of the ranking metric. Values above the plot represent the normalized enrichment score (NES) and FDR-corrected significance. (FIG. 6B) Left, percentage of cells that were clonally expanded in TIM3⁺ (HAVCR2>10 TPM) TRM cells, remaining TRMs and non-T_(RM); clonal expansion was determined for cells from 4 and 2 patients for TRM and non-T_(RM), respectively. Right, clonotype network graphs of cells from a representative donor. TIM3⁺ (HAVCR2>10 TPM) TRM cells are marked with a circle; cells with greater than 10 T_(RM) expression of either MKI67 or TOP2A were considered cycling and denoted with an 6 asterisk. (FIG. 6C) Violin plot of expression of indicated transcripts; Shape represents the distribution of expression among cells and color represents average expression, calculated from the TPM. (FIG. 6D) Correlation of PDCD1 and IFNG expression in TIM3+ TRM and non-T_(RM) cells; each dot represents a cell. Percentages indicate the percentage of cells inside each of the graph sections (r indicates Spearman correlation value; ** P≤0.01, ns=no significance). (FIG. 6E) Spearman co-expression analysis of genes whose expression is enriched in the ‘hyper functional’ TRM cluster (FIG. 5A) in tumor TRM and non-T_(RM) populations, respectively; matrix is clustered according to gene linkage. (FIG. 6F) tSNE visualization of flow cytometry data from 3,000 randomly selected live and singlet-gated CD19⁻CD20⁻CD14⁻CD56⁻CD4⁻CD45⁺CD3⁺CD8⁺ cells isolated from 8 paired tumor and lung samples; each cell is represented by a dot colored as TRM or non-T_(RM) (left), tumor or lung (second left), and according to Z-score expression value of the protein indicated above the plot (remaining panels). (FIG. 6G) Applicants' plots show expression of TIM3 versus IL7R in the cell type and tissue indicated above the plot; percentage of TIM3⁺ cells in the indicated populations is shown (right), each symbol represents an individual sample; the small line indicates the s.e.m, bars are mean and colored as indicated (*P≤0.05; n=8). (FIG. 6H) Right, geometric mean fluorescent intensity (GMFI) of CD39, PD1 and 41BB for each tumor TRM subset; bars represent the mean, t-line the s.e.m., and symbol represents data from individual samples (**P≤0.01; n=8); representative histograms shown (left). (FIG. 6I) Co-expression analysis of flow cytometry data (FIG. 6F), as per Spearman correlation value, matrix is clustered by complete linkage. (FIG. 6J) Spearman correlation of HACVR2 (TIM3) expression with ITGAE (CD103) expression in bulk transcriptomic profiles of CTLs isolated from lung cancer and head and neck squamous cell carcinoma.

FIG. 7: Cell sorting strategy. Plots describe the sorting strategy used for isolating immune cell types from tissue samples.

FIG. 8: Validation of lung TRM phenotype. Flow-cytometry analysis of the expression of KLRG1 and CD49A versus that of CD103 in live, singlet CD19⁻CD20⁻CD14⁻CD45⁺CD3⁺CD8⁺ cells obtained from lung samples (n=6).

FIG. 9: Validation of PD1 expression. Flow-cytometry analysis of the expression of PD-1 versus that of CD103 in live, singlet CD19⁻CD20⁻CD14⁻CD4⁻CD56⁻CD45⁺CD3⁺CD8⁺ cells obtained from lung and tumor samples (n=8).

FIGS. 10A-10D: T_(RM) cluster into 4 major subtypes. (FIG. 10A) Principle component analysis of the single cell transcriptomes, each point represents a cell which are colored as per the cluster assignment in FIG. 5; numbers along perimeter indicate principal components (PC1-PC3). (FIG. 10B) tSNE visualization of single cell transcriptomes, shown per donor (as per FIG. 4a ), obtained from 12 tumors and 6 matched normal lung samples. Each symbol represents a cell; color indicates Seurat clustering of cells, as per FIG. 4b , identifying 9 clusters. (FIG. 10C) Breakdown of cells assigned to each cluster in each donor, separated by tissue type of origin (colored as per FIG. 4B). (FIG. 10D) The distance between a cell assigned to cluster 1 compared to the mean of cells assigned into the other clusters (colored as per b). The difference was calculated with the raw (left) and z-score normalized (right) distances, bars represent the mean distance to each of the other clusters, t-line the s.e.m., and symbols represent individual cells in cluster 1 (**** P≤0.0001; Wilcoxon matched-pairs signed rank test, n=135 cells).

FIGS. 11A-11B: ‘Highly-functional’ TRM cells are enriched for transcripts associated with enhanced anti-tumor features. (FIG. 11A) Violin plot of expression of indicated transcripts; shape represents the distribution of expression among cells and color represents average expression, calculated from the Seurat-normalized counts. (FIG. 11B) SAVER-imputed spearman co-expression analysis of genes whose expression is enriched in the TIM-3+IL7R− TRM cluster (FIG. 5A) in tumor TRM and non-TRM clusters, respectively; matrix is clustered according to complete linkage.

FIG. 12: TIM3-expressing TRM cells are enriched for co-expression of PD1 and cytotoxicity-related transcripts. Single-cell RNA-Seq analysis of transcripts (one per row) differentially expressed by TIM3⁺ ^(TRM) relative to non-TIM3⁺ (MAST analysis with an adjusted P value of <0.05), presented as row-wise z-scores of TPM; each column represents a single cell (n=89 and 411, respectively).

FIGS. 13A-13C: Tumor TRMS are enriched for TIM3⁺ cells. (FIG. 13A) Flow-cytometry analysis of the expression of TIM3 versus that of CD103 in live, singlet CD19⁻CD20⁻CD14⁻CD4⁻CD56⁻CD45⁺CD3⁺CD8⁺ cells obtained from lung, lung tumor and HNSCC samples (n=8,8,3, respectively). (FIG. 13B) Flow-cytometry analysis of TIM3 compared to IL7R in CD103⁺ cells gated as in (FIG. 13A). (FIG. 13C) Quantification (geometric mean) of indicated marker (above) in HNSCC cells gated as in (FIG. 13B), bars represent the mean, t-line the s.e.m., and symbol represents data from individual samples (n=3).

FIG. 14: Analysis of AMICA1 expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Middle) Percent of cells expressing a given transcript in each cluster. (Lower) Expression values according to the log₂+1 transformed RNA-seq transcripts per million of the indicated differentially expressed genes shared by lung and tumor TRM cells. Each symbol represents an individual sample, the bar represents the mean and colored as described in the legend, t-line the s.e.m.

FIG. 15: Analysis of SPRY1 expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Middle) Percent of cells expressing a given transcript in each cluster. (Lower) Expression values according to the log₂+1 transformed RNA-seq transcripts per million of the indicated differentially expressed genes shared by lung and tumor TRM cells. Each symbol represents an individual sample, the bar represents the mean and colored as described in the legend, t-line the s.e.m.

FIG. 16: Analysis of CHN1 expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 17: Analysis of PAG1 expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 18: Analysis of PTPN22 expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 19: Analysis of DUSP4 expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 20: Analysis of ICOS expression. (Upper) tSNE visualization of ˜42,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 21: Analysis of TNFRSF18 (GITR) expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 22: Analysis of TMIGD2 (CD28H) expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3±CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Middle) Percent of cells expressing a given transcript in each cluster. (Lower) Expression values according to the log₂+1 transformed RNA-seq transcripts per million of the indicated differentially expressed genes shared by lung and tumor TRM cells. Each symbol represents an individual sample, the bar represents the mean and colored as described in the legend, t-line the s.e.m.

FIG. 23: Analysis of CD226 expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Middle) Percent of cells expressing a given transcript in each cluster. (Lower) Expression values according to the log₂+1 transformed RNA-seq transcripts per million of the indicated differentially expressed genes shared by lung and tumor TRM cells. Each symbol represents an individual sample, the bar represents the mean and colored as described in the legend, t-line the s.e.m.

FIG. 24: Analysis of TIGIT expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 25: Analysis of KLRC1 (NKG2A) expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Middle) Percent of cells expressing a given transcript in each cluster. (Lower) Expression values according to the log₂+1 transformed RNA-seq transcripts per million of the indicated differentially expressed genes shared by lung and tumor TRM cells. Each symbol represents an individual sample, the bar represents the mean and colored as described in the legend, t-line the s.e.m.

FIG. 26: Analysis of KLRC2 (NKG2C) expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Middle) Percent of cells expressing a given transcript in each cluster. (Lower) Expression values according to the log₂+1 transformed RNA-seq transcripts per million of the indicated differentially expressed genes shared by lung and tumor T_(RM) cells. Each symbol represents an individual sample, the bar represents the mean and colored as described in the legend, t-line the s.e.m.

FIG. 27: Analysis of CAPG expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Middle) Percent of cells expressing a given transcript in each cluster. (Lower) Expression values according to the log₂+1 transformed RNA-seq transcripts per million of the indicated differentially expressed genes shared by lung and tumor TRM cells. Each symbol represents an individual sample, the bar represents the mean and colored as described in the legend, t-line the s.e.m.

FIG. 28: Analysis of MYO1E expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 29: Analysis of CLEC2B expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 30: Analysis of CLECL1 expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 31: Analysis of TNFRSF9 (4-1BB/CD137) expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 32: Analysis of TNFSF4 (CD134L/OX40L) expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 33: Analysis of NR3C1 (glucocorticoid receptor) expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 34: Analysis of CD7 expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 35: Analysis of KLRD1 (CD94) expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 36: Analysis of CLEC2D expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 37: Analysis of ITM2A expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 38: Analysis of VCAM1 (CD106) expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 39: Analysis of KRT81 expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 40: Analysis of KRT86 expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 41: Analysis of CXCL13 expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 42: Analysis of CBLB expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 43: Analysis of KLRC3 (NKG2-E) expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Middle) Percent of cells expressing a given transcript in each cluster. (Lower) Expression values according to the log₂+1 transformed RNA-seq transcripts per million of the indicated differentially expressed genes shared by lung and tumor T_(RM) cells. Each symbol represents an individual sample, the bar represents the mean and colored as described in the legend, t-line the s.e.m.

FIG. 44: Analysis of KLRB1 (CD161) expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Middle) Percent of cells expressing a given transcript in each cluster. (Lower) Expression values according to the log₂+1 transformed RNA-seq transcripts per million of the indicated differentially expressed genes shared by lung and tumor TRM cells. Each symbol represents an individual sample, the bar represents the mean and colored as described in the legend, t-line the s.e.m.

FIG. 45: Analysis of CD101 expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Middle) Percent of cells expressing a given transcript in each cluster. (Lower) Expression values according to the log₂+1 transformed RNA-seq transcripts per million of the indicated differentially expressed genes shared by lung and tumor TRM cells. Each symbol represents an individual sample, the bar represents the mean and colored as described in the legend, t-line the s.e.m.

FIG. 46: Analysis of CD101 expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Middle) Percent of cells expressing a given transcript in each cluster. (Lower) Expression values according to the log₂+1 transformed RNA-seq transcripts per million of the indicated differentially expressed genes shared by lung and tumor TRM cells. Each symbol represents an individual sample, the bar represents the mean and colored as described in the legend, t-line the s.e.m.

FIG. 47: Analysis of CD200R1 expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Middle) Percent of cells expressing a given transcript in each cluster. (Lower) Expression values according to the log₂+1 transformed RNA-seq transcripts per million of the indicated differentially expressed genes shared by lung and tumor TRM cells. Each symbol represents an individual sample, the bar represents the mean and colored as described in the legend, t-line the s.e.m.

FIG. 48: Analysis of SLA (SLAP) expression. (Upper) tSNE visualization of ˜12,000 live and singlet-gated CD14⁻CD19⁻CD20⁻CD4⁻CD56⁻CD3⁺CD45⁺CD8⁺ single cell transcriptomes obtained from 12 tumors and 6 matched normal lung samples, the TIM3⁺IL7R⁻ T_(RM) cluster is found in the bottom right. Expression is calculated using Seurat normalized counts. (Lower) Percent of cells expressing a given transcript in each cluster.

FIG. 49: CD103 density predicts survival in lung cancer. CD103 density (CD103high, CD103int, CD103low) in tumors pre-classified based on CD8 density (left); Kaplan-Meier curves for lung cancer mortality in CD8high tumors sub-classified according to density of CD103 (right).

FIGS. 50A-50B: (FIG. 50 A) Flow-cytometry analysis of the percentage of PD-1+T_(RM) and PD-1+ non-TRM cells that express effector cytokines following 4 hours of ex-vivo stimulation. Gated on live and singlet-gated CD14−CD20−CD4−CD45+CD3+CD8+ cells obtained from lung cancer TILs, discriminated on CD103 expression (**≤0.01; Wilcoxon matched pairs signed rank test; n=11), each symbol represents a sample. Surface molecules (e.g., PD-1) were stained before stimulation. (FIG. 50 B) Analysis of Granzyme A and Granzyme B directly ex-vivo, gated and analyzed as per a) (*** P≤0.001).

FIG. 51: Left, quantification of the number of CD8A+CD103+TIM-3+ cells per region in biopsies defined as having a TILhigh/TRM high status versus. TILlow/TRM low status. Right, percent of CD8A+CD103+ CTLs expressing TIM-3 in each clinical subtype. Bars represent the mean, t-line the s.e.m., and symbols represent individual data points (* P≤0.05; **** P≤0.0001; n=21).

FIGS. 52A-52K: (FIG. 52A) Representative FACS plots to characterize tumor-infiltrating CD19⁺, CD4⁺ and CD8⁺ T cells from mice at d21 after inoculation with B16F10-OVA cells. (FIG. 52B, FIG. 52C) MFI of AMICA1 expression of CD19⁺, CD4⁺and CD8⁺ TILs as in (FIG. 52A). (FIG. 52D) Frequency of AMICA1 expressing CD19⁺, CD4⁺ and CD8⁺ TILs as in (FIG. 52A). (FIG. 52E, FIG. 52F) Representative FACS plots (FIG. 52E) depicting cell viability, electroporation efficiency, antigen specificity and knockdown efficiency (FIG. 52F) of purified, in vitro activated and electroporated OT-I CD8⁺ T cells at 96 h after electroporation. Cells were electroporated to introduce gRNAs targeting a control region (ctrl) or AMICA1. (FIG. 52G, FIG. 52H, FIG. 52I) Representative FACS plots from mice at d20 after inoculation with B16F10-OVA cells. CD45.2 OT-I control and AMICA-1^(−/−) T cells were adoptively transferred at d6 after tumor inoculation. (FIG. 52J) Growth curves of B16F10-OVA tumors after adoptive transfer of OT-I and AMICA-1^(−/−) T cells. (FIG. 52K) Growth curves of B16F10-OVA tumors after treatment at d10 and d13 with 200 ug anti-PD-1, anti-AMICA-1 or anti isotype control antibodies.

FIG. 53: Left, quantification of the number of CD8A+CD103+TIM-3+ cells per region in biopsies defined as having a TILhigh/TRM high status versus. TILlow/TRM low status. Right, percent of CD8A+CD103+ CTLs expressing TIM-3 in each clinical subtype. Bars represent the mean, t-line the s.e.m., and symbols represent individual data points (* P≤0.05; **** P≤0.0001; n=21).

FIGS. 54A-54H: Single-cell transcriptome analysis. Left, contour plots show the expression of TIM-3 and IL-7R in CD14−CD19−CD20−CD4−CD45+CD3+CD8+CD103+ cells isolated from patients receiving anti-PD-1 treatment, at the time point indicated above the plot (TP); number in bottom right indicates the percentage of tumor T_(RM) cells (CD8+CD103+) with TIM-3+IL-7R− surface phenotype. Right, quantification of the percentage of tumor-infiltrating TIM-3+IL-7R− TRM cells, isolated from the anti-PD1 responding, non-responding and treatment naïve patients (FIG. 56G). Bars represent the mean, t-line the s.e.m., and symbols represent individual data points (* P≤0.05; ** P≤0.01; n=7, 8 and 12 biopsies for responders, treatment naïve and nonresponders, respectively). (FIG. 54B) Contour plots demonstrate the expression of TIM-3 and PD-1 in the TRM cells isolated from pre-immunotherapy biopsies (gated as per FIG. 54A). (FIG. 54C) Singlecell RNA-seq analysis of transcripts (one per row) differentially expressed between CTLs pre- and post-anti-PD-1 (MAST analysis), with an adjusted P value of <0.05), presented as row-wise z-scores of TPM counts; each column represents a single cell (n=127 and 151 cells, respectively). (FIG. 54D) Violin plot of expression of indicated transcripts differentially expressed between tumor-infiltrating CTLs isolated from pre- and post-anti-PD-1 treatment samples (as per FIG. 54C); shape represents the distribution of expression among cells and color represents average expression, calculated from the TPM counts. (FIG. 54E) GSEA of the bulk tumor CD103+ versus. CD103− transcriptional signature (FIG. 3a ) and TIM-3+IL7R− TRM cell 29 signature (FIG) in tumor-infiltrating CTLs isolated from pre- and post-anti-PD-1 treatment samples: top, running enrichment score (RES) for the gene set, from most enriched at the left to most under-represented at the right; middle, positions of gene set members (blue vertical lines) in the ranked list of genes; bottom, value of the ranking metric. Values above the plot represent the normalized enrichment score (NES) and FDR-corrected significance. (FIG. 54F) Spearman co-expression analysis of transcripts enriched in tumor-infiltrating CTLs from post-anti-PD-1 treatment samples (c); matrix is clustered according to complete linkage. (FIG. 54G) Correlation analysis of all peaks identified in the OMNI-ATAC-seq libraries, pooled from 9 donors across two experiments, cells were sorted on CD14−CD19−CD20−CD4−CD45+CD3+CD8+CD103+TIM-3+IL-7R− and CD14−CD19−CD20−CD4−CD45+CD3+CD8+CD103−. Matrix is clustered according to complete linkage. (FIG. 54H) University of California Santa Cruz genome browser tracks for key TRM-associated gene loci as indicated above the tracks. RNA-seq tracks are merged from all purified bulk RNA-seq data, presented as Reads Per Kilobase Million (RPKM) (as per FIG. 2B; tumor non-TRM=25, tumor TRM=19; OMNI-ATACseq as per FIG. 54G).

FIGS. 55A-55C: Validation of T_(RM) phenotype. (FIG. 55A) Flow-cytometry contour plots showing the expression of CD49A and KLRG1 versus. that of CD103 in live, singlet, CD14−CD19−CD20−CD4−CD45+CD3+CD8+ cells obtained from lung samples (n=6). (FIG. 55B) GSEA of the murine composite TRM signature in the transcriptome of TRM versus. non-TRM: top, running enrichment score (RES) for the gene set, from most enriched genes at left to most underrepresented at right; middle, positions of gene set members (blue vertical lines) in the ranked list of genes; bottom, value of the ranking metric. Values above the plot represent the normalized enrichment score (NES) and false discovery rate (FDR)-corrected significance value in CTLs isolated from lung and tumor samples. (FIG. 55C) GSEA of the lung TRM versus. non-TRM cells for non-preserved transcripts (in FIG. 1E, FIG. 1F; as per e; N/S=Not significant).

FIGS. 56A-56B: PD-1 is co-expressed with cytotoxicity associated molecules at the protein level ex-vivo. (FIG. 56A) Flow-cytometry analysis of PD-1+ TRM and non-TRM cells versus. a particular cytokine (as indicated below the plots) following 4 hours of ex-vivo stimulation. Gated on live and singlet-gated CD14−CD20−CD4−CD45+CD3+CD8+ cells obtained from lung cancer TILs (FIG. 56B) Analysis of Granzyme A and Granzyme B directly exvivo, Gated and analyzed, as per (FIG. 56A).

FIG. 57: TIM-3+IL7R− TRM cells are enriched in responders to anti-PD-1 therapy. Flow-cytometry analysis of the expression of TIM-3 versus. that of IL-7R in live, singlet CD14CD20−CD4−CD45+CD3+CD8+CD103+ cells obtained from patients responding or not-responding to anti-PD-1 therapy (n=18).

FIGS. 58A-58D: Single-cell transcriptome analysis of CTLs from anti-PD-1 responders. (FIG. 58A) Schematic representation of clinical details and cells sorted for the patients selected for study (time point—TP). (FIG. 58B) Example of in-silico removal of CD4+ cells, highlighting the transcriptomic drop outs. The dashed line corresponds to the CD4+ cells removed. (FIG. 58C) A clonotype network graph of cells from (FIG. 58A), highlighting the time point from which the cells were isolated. Cells highlighted through a dashed line correspond to shared clonotypes across time points. (FIG. 58D) A clonotype network graph (as per c), highlighting the TRM cells and non-TRM cells, marked respectively. Cells were assigned based on protein expression of CD103, alternatively if cell-specific protein expression was not available, cells with greater than 10 TPM counts expression of either ITGAE (CD103), RBPJ or ZNF683 (HOBIT) considered a TRM.

TABLES

Table 1. List of prioritized genes Table 2. Expanded list of prioritized genes Table 3. List of differentially expressed genes in Lung TRM from non-TRM Table 4. List of differentially expressed genes in tumor TRM from tumor non-TRM Table 5. List of uniquely expressed genes in tumor TRM Table 6. TCR-seq library and clonality information Table 7. List of uniquely expressed genes in tumor TRM subtypes

DETAILED DESCRIPTION

It is to be understood that the present disclosure is not limited to particular aspects described, as such may, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting, since the scope of the present disclosure will be limited only by the appended claims.

A number of embodiments of the disclosure have been described. Nevertheless, it will be understood that various modifications may be made without departing from the spirit and scope of the disclosure. Accordingly, the following examples are intended to illustrate but not limit the scope of disclosure described in the claims.

It is to be inferred without explicit recitation and unless otherwise intended, that when the present technology relates to a polypeptide, protein, polynucleotide or antibody, an equivalent or a biologically equivalent of such is intended within the scope of the present technology.

Throughout this disclosure, various publications, patents and published patent specifications are referenced by an identifying citation. The full bibliographic information for the citations is found immediately preceding the claims. All publications, patent applications, patents, and other references mentioned herein are expressly incorporated by reference in their entirety, to the same extent as if each were incorporated by reference individually. In case of conflict, the present specification, including definitions, will control.

The entirety of each patent, patent application, publication or any other reference or document cited herein hereby is incorporated by reference. In case of conflict, the specification, including definitions, will control.

Citation of any patent, patent application, publication or any other document is not an admission that any of the foregoing is pertinent prior art, nor does it constitute any admission as to the contents or date of these publications or documents.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present disclosure, suitable methods and materials are described herein.

All of the features disclosed herein may be combined in any combination. Each feature disclosed in the specification may be replaced by an alternative feature serving a same, equivalent, or similar purpose. Thus, unless expressly stated otherwise, disclosed features (e.g., antibodies) are an example of a genus of equivalent or similar features.

As used herein, all numerical values or numerical ranges include integers within such ranges and fractions of the values or the integers within ranges unless the context clearly indicates otherwise. Further, when a listing of values is described herein (e.g., about 50%, 60%, 70%, 80%, 85% or 86%) the listing includes all intermediate and fractional values thereof (e.g., 54%, 85.4%). Thus, to illustrate, reference to 80% or more identity, includes 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94% etc., as well as 81.1%, 81.2%, 81.3%, 81.4%, 81.5%, etc., 82.1%, 82.2%, 82.3%, 82.4%, 82.5%, etc., and so forth.

Reference to an integer with more (greater) or less than includes any number greater or less than the reference number, respectively. Thus, for example, a reference to less than 100, includes 99, 98, 97, etc. all the way down to the number one (1); and less than 10, includes 9, 8, 7, etc. all the way down to the number one (1).

As used herein, all numerical values or ranges include fractions of the values and integers within such ranges and fractions of the integers within such ranges unless the context clearly indicates otherwise. Thus, to illustrate, reference to a numerical range, such as 1-10 includes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, as well as 1.1, 1.2, 1.3, 1.4, 1.5, etc., and so forth. Reference to a range of 1-50 therefore includes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, etc., up to and including 50, as well as 1.1, 1.2, 1.3, 1.4, 1.5, etc., 2.1, 2.2, 2.3, 2.4, 2.5, etc., and so forth.

Reference to a series of ranges includes ranges which combine the values of the boundaries of different ranges within the series. Thus, to illustrate reference to a series of ranges, for example, of 1-10, 10-20, 20-30, 30-40, 40-50, 50-60, 60-75, 75-100, 100-150, 150-200, 200-250, 250-300, 300-400, 400-500, 500-750, 750-1,000, 1,000-1,500, 1,500-2,000, 2,000-2,500, 2,500-3,000, 3,000-3,500, 3,500-4,000, 4,000-4,500, 4,500-5,000, 5,500-6,000, 6,000-7,000, 7,000-8,000, or 8,000-9,000, includes ranges of 10-50, 50-100, 100-1,000, 1,000-3,000, 2,000-4,000, etc.

Modifications can be made to the foregoing without departing from the basic aspects of the technology. Although the technology has been described in substantial detail with reference to one or more specific embodiments, those of ordinary skill in the art will recognize that changes can be made to the embodiments specifically disclosed in this application, yet these modifications and improvements are within the scope and spirit of the technology.

The disclosure is generally disclosed herein using affirmative language to describe the numerous embodiments and aspects. The disclosure also specifically includes embodiments in which particular subject matter is excluded, in full or in part, such as substances or materials, method steps and conditions, protocols, or procedures. For example, in certain embodiments or aspects of the disclosure, materials and/or method steps are excluded. Thus, even though the disclosure is generally not expressed herein in terms of what the disclosure does not include aspects that are not expressly excluded in the disclosure are nevertheless disclosed herein.

The technology illustratively described herein suitably can be practiced in the absence of any element(s) not specifically disclosed herein. Thus, for example, in each instance herein any of the terms “comprising,” “consisting essentially of,” and “consisting of” can be replaced with either of the other two terms. The terms and expressions which have been employed are used as terms of description and not of limitation and use of such terms and expressions do not exclude any equivalents of the features shown and described or segments thereof, and various modifications are possible within the scope of the technology claimed. The term “a” or “an” can refer to one of or a plurality of the elements it modifies (e.g., “a reagent” can mean one or more reagents) unless it is contextually clear either one of the elements or more than one of the elements is described. The term “about” as used herein refers to a value within 10% of the underlying parameter (i.e., plus or minus 10%), and use of the term “about” at the beginning of a string of values modifies each of the values (i.e., “about 1, 2 and 3” refers to about 1, about 2 and about 3). For example, a weight of “about 100 grams” can include weights between 90 grams and 110 grams. The term “substantially” as used herein refers to a value modifier meaning “at least 95%”, “at least 96%”, “at least 97%”, “at least 98%”, or “at least 99%” and may include 100%. For example, a composition that is substantially free of X, may include less than 5%, less than 4%, less than 3%, less than 2%, or less than 1% of X, and/or X may be absent or undetectable in the composition.

Thus, it should be understood that although the present technology has been specifically disclosed by representative embodiments and optional features, modification and variation of the concepts herein disclosed can be resorted to by those skilled in the art, and such modifications and variations are considered within the scope of this technology.

Definitions

As used in the specification and claims, the singular form “a”, “an”, and “the” include plural references unless the context clearly dictates otherwise. For example, the term “a cell” includes a plurality of cells, including mixtures thereof.

As used herein, the term “comprising” is intended to mean that the compositions or methods include the recited steps or elements, but do not exclude others. “Consisting essentially of” shall mean rendering the claims open only for the inclusion of steps or elements, which do not materially affect the basic and novel characteristics of the claimed compositions and methods. “Consisting of” shall mean excluding any element or step not specified in the claim. Embodiments defined by each of these transition terms are within the scope of this disclosure.

As used herein, the term “about” is used to indicate that a value includes the standard deviation of error for the device or method being employed to determine the value. The term “about” when used before a numerical designation, e.g., temperature, time, amount, and concentration, including range, indicates approximations which may vary by (+) or (−) 15%, 10%, 5%, 3%, 2%, or 1%.

As used herein, the term “animal” refers to living multi-cellular vertebrate organisms, a category that includes, for example, mammals and birds. The term “mammal” includes both human and non-human mammals, e.g., bovines, canines, felines, rat, murines, simians, equines and humans. Additional examples include adults, juveniles and infants

The term “subject,” “host,” “individual,” and “patient” are as used interchangeably herein to refer to animals, typically mammalian animals. Any suitable mammal can be treated by a method, cell or composition described herein. Non-limiting examples of mammals include humans, non-human primates (e.g., apes, gibbons, chimpanzees, orangutans, monkeys, macaques, and the like), domestic animals (e.g., dogs and cats), farm animals (e.g., horses, cows, goats, sheep, pigs) and experimental animals (e.g., mouse, rat, rabbit, guinea pig). In some embodiments a mammal is a human. A mammal can be any age or at any stage of development (e.g., an adult, teen, child, infant, or a mammal in utero). A mammal can be male or female. A mammal can be a pregnant female. In some embodiments a subject is a human. In some embodiments, a subject has or is suspected of having a cancer or neoplastic disorder.

“Eukaryotic cells” comprise all of the life kingdoms except monera. They can be easily distinguished through a membrane-bound nucleus. Animals, plants, fungi, and protists are eukaryotes or organisms whose cells are organized into complex structures by internal membranes and a cytoskeleton. The most characteristic membrane-bound structure is the nucleus. Unless specifically recited, the term “host” includes a eukaryotic host, including, for example, yeast, higher plant, insect and mammalian cells. Non-limiting examples of eukaryotic cells or hosts include simian, bovine, porcine, murine, rat, avian, reptilian and human.

“Prokaryotic cells” usually lack a nucleus or any other membrane-bound organelles and are divided into two domains, bacteria and archaea. In addition to chromosomal DNA, these cells can also contain genetic information in a circular loop called on episome. Bacterial cells are very small, roughly the size of an animal mitochondrion (about 1-2 μm in diameter and 10 μm long). Prokaryotic cells feature three major shapes: rod shaped, spherical, and spiral. Instead of going through elaborate replication processes like eukaryotes, bacterial cells divide by binary fission. Examples include but are not limited to Bacillus bacteria, E. coli bacterium, and Salmonella bacterium.

As used herein “a population of cells” intends a collection of more than one cell that is identical (clonal) or non-identical in phenotype and/or genotype.

As used herein, “substantially homogenous” population of cells is a population having at least 70%, or alternatively at least 75%, or alternatively at least 80%, or alternatively at least 85%, or alternatively at least 90%, or alternatively at least 95%, or alternatively at least 98% identical phenotype, as measured by pre-selected markers, phenotypic or genomic traits. In one aspect, the population is a clonal population.

As used herein, “heterogeneous” population of cells is a population having up to 69%, or alternatively up to 60%, or alternatively up to 50%, or alternatively up to 40%, or alternatively up to 30%, or alternatively up to 20%, or alternatively up to 10%, or alternatively up to 5%, or alternatively up to 4%, or alternatively up to 3%, or alternatively up to 2%, or alternatively up to 61%, or alternatively up to 0.5% identical phenotype, as measured by pre-selected markers, phenotypic or genomic traits.

A “composition” typically intends a combination of the active agent, e.g., an engineered immune cell, e.g. T-cell, a modified T-cell, a NK cell, a chimeric antigen cell, a cell comprising an engineered immune cell, e.g. a T-cell, a NK cell, a CART cell or a CAR NK cell, an antibody, a cytokine, IL-12, a compound or composition, and a naturally-occurring or non-naturally-occurring carrier, inert (for example, a detectable agent or label) or active, such as an adjuvant, diluent, binder, stabilizer, buffers, salts, lipophilic solvents, preservative, adjuvant or the like and include pharmaceutically acceptable carriers. Carriers also include pharmaceutical excipients and additives proteins, peptides, amino acids, lipids, and carbohydrates (e.g., sugars, including monosaccharides, di-, tri, tetra-oligosaccharides, and oligosaccharides; derivatized sugars such as alditols, aldonic acids, esterified sugars and the like; and polysaccharides or sugar polymers), which can be present singly or in combination, comprising alone or in combination 1-99.99% by weight or volume. Exemplary protein excipients include serum albumin such as human serum albumin (HSA), recombinant human albumin (rHA), gelatin, casein, and the like. Representative amino acid components, which can also function in a buffering capacity, include alanine, arginine, glycine, arginine, betaine, histidine, glutamic acid, aspartic acid, cysteine, lysine, leucine, isoleucine, valine, methionine, phenylalanine, aspartame, and the like. Carbohydrate excipients are also intended within the scope of this technology, examples of which include but are not limited to monosaccharides such as fructose, maltose, galactose, glucose, D-mannose, sorbose, and the like; disaccharides, such as lactose, sucrose, trehalose, cellobiose, and the like; polysaccharides, such as raffinose, melezitose, maltodextrins, dextrans, starches, and the like; and alditols, such as mannitol, xylitol, maltitol, lactitol, xylitol sorbitol (glucitol) and myoinositol.

The compositions used in accordance with the disclosure, including cells, treatments, therapies, agents, drugs and pharmaceutical formulations can be packaged in dosage unit form for ease of administration and uniformity of dosage. The term “unit dose” or “dosage” refers to physically discrete units suitable for use in a subject, each unit containing a predetermined quantity of the composition calculated to produce the desired responses in association with its administration, i.e., the appropriate route and regimen. The quantity to be administered, both according to number of treatments and unit dose, depends on the result and/or protection desired. Precise amounts of the composition also depend on the judgment of the practitioner and are peculiar to each individual. Factors affecting dose include physical and clinical state of the subject, route of administration, intended goal of treatment (alleviation of symptoms versus cure), and potency, stability, and toxicity of the particular composition. Upon formulation, solutions will be administered in a manner compatible with the dosage formulation and in such amount as is therapeutically or prophylactically effective. The formulations are easily administered in a variety of dosage forms, such as the type of injectable solutions described herein.

As used herein, the terms “nucleic acid sequence” and “polynucleotide” are used interchangeably to refer to a polymeric form of nucleotides of any length, either ribonucleotides or deoxyribonucleotides. Thus, this term includes, but is not limited to, single-, double-, or multi-stranded DNA or RNA, genomic DNA, cDNA, DNA-RNA hybrids, or a polymer comprising purine and pyrimidine bases or other natural, chemically or biochemically modified, non-natural, or derivatized nucleotide bases.

The term siRNA intends short hairpin RNAs (shRNAs). shRNAs comprise a single strand of RNA that forms a stem-loop structure, where the stem consists of the complementary sense and antisense strands that comprise a double-stranded siRNA, and the loop is a linker of varying size. The stem structure of shRNAs generally is from about 10 to about 30 nucleotides long.

The term microRNAs (miRNAs) intends a class of small noncoding RNAs of about 22 nucleotide in length which are involved in the regulation of gene expression at the posttranscriptional level by degrading their target mRNAs and/or inhibiting their translation.

The term “encode” as it is applied to nucleic acid sequences refers to a polynucleotide which is said to “encode” a polypeptide if, in its native state or when manipulated by methods well known to those skilled in the art, can be transcribed and/or translated to produce the mRNA for the polypeptide and/or a fragment thereof. The antisense strand is the complement of such a nucleic acid, and the encoding sequence can be deduced therefrom.

As used herein, the term “isolated cell” generally refers to a cell that is substantially separated from other cells of a tissue. The term includes prokaryotic and eukaryotic cells.

“Immune cells” includes, e.g., white blood cells (leukocytes) which are derived from hematopoietic stem cells (HSC) produced in the bone marrow, lymphocytes (T cells, B cells, natural killer (NK) cells) and myeloid-derived cells (neutrophil, eosinophil, basophil, monocyte, macrophage, dendritic cells). “T cell” includes all types of immune cells expressing CD3 including T-helper cells (CD4+ cells), cytotoxic T-cells (CD8+ cells), natural killer T-cells, T-regulatory cells (Treg) and gamma-delta T cells. A “cytotoxic cell” includes CD8+ T cells, natural-killer (NK) cells, and neutrophils, which cells are capable of mediating cytotoxicity responses. Cytokines are small secreted proteins released by immune cells that have a specific effect on the interactions and communications between the immune cells. Cytokines can be pro-inflammatory or anti-inflammatory. Non-limiting example of a cytokine is Granulocyte-macrophage colony-stimulating factor (GM-CSF), which stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes.

As used herein, the phrase “immune response” or its equivalent “immunological response” or “anti-tumor response” refers to the development of a cell-mediated response (e.g. mediated by antigen-specific T cells or their secretion products). A cellular immune response is elicited by the presentation of polypeptide epitopes in association with Class I or Class II MHC molecules, to treat or prevent a viral infection, expand antigen-specific B-reg cells, TC1, CD4+T helper cells and/or CD8+ cytotoxic T cells and/or disease generated, autoregulatory T cell and B cell “memory” cells. The response may also involve activation of other components. In some aspect, the term “immune response” may be used to encompass the formation of a regulatory network of immune cells. Thus, the term “regulatory network formation” may refer to an immune response elicited such that an immune cell, preferably a T cell, more preferably a T regulatory cell, triggers further differentiation of other immune cells, such as but not limited to, B cells or antigen-presenting cells—non-limiting examples of which include dendritic cells, monocytes, and macrophages. In certain embodiments, regulatory network formation involves B cells being differentiated into regulatory B cells; in certain embodiments, regulatory network formation involves the formation of tolerogenic antigen-presenting cells.

The term “transduce” or “transduction” as it is applied to the production of chimeric antigen receptor cells refers to the process whereby a foreign nucleotide sequence is introduced into a cell. In some embodiments, this transduction is done via a vector.

As used herein, the term “vector” refers to a nucleic acid construct deigned for transfer between different hosts, including but not limited to a plasmid, a virus, a cosmid, a phage, a BAC, a YAC, etc. A “viral vector” is defined as a recombinantly produced virus or viral particle that comprises a polynucleotide to be delivered into a host cell, either in vivo, ex vivo or in vitro. In some embodiments, plasmid vectors may be prepared from commercially available vectors. In other embodiments, viral vectors may be produced from baculoviruses, retroviruses, adenoviruses, AAVs, etc. according to techniques known in the art. In one embodiment, the viral vector is a lentiviral vector. Examples of viral vectors include retroviral vectors, adenovirus vectors, adeno-associated virus vectors, alphavirus vectors and the like. Infectious tobacco mosaic virus (TMV)-based vectors can be used to manufacturer proteins and have been reported to express Griffithsin in tobacco leaves (O'Keefe et al. (2009) Proc. Nat. Acad. Sci. USA 106(15):6099-6104). Alphavirus vectors, such as Semliki Forest virus-based vectors and Sindbis virus-based vectors, have also been developed for use in gene therapy and immunotherapy. See, Schlesinger & Dubensky (1999) Curr. Opin. Biotechnol. 5:434-439 and Ying et al. (1999) Nat. Med. 5(7):823-827. Further details as to modern methods of vectors for use in gene transfer may be found in, for example, Kotterman et al. (2015) Viral Vectors for Gene Therapy: Translational and Clinical Outlook Annual Review of Biomedical Engineering 17. Vectors that contain both a promoter and a cloning site into which a polynucleotide can be operatively linked are well known in the art. Such vectors are capable of transcribing RNA in vitro or in vivo and are commercially available from sources such as Agilent Technologies (Santa Clara, Calif.) and Promega Biotech (Madison, Wis.).

An “an effective amount” or “efficacious amount” is an amount sufficient to achieve the intended purpose, non-limiting examples of such include: initiation of the immune response, modulation of the immune response, suppression of an inflammatory response and modulation of T cell activity or T cell populations. In one aspect, the effective amount is one that functions to achieve a stated therapeutic purpose, e.g., a therapeutically effective amount. As described herein in detail, the effective amount, or dosage, depends on the purpose and the composition, and can be determined according to the present disclosure.

As used herein, the term “T cell,” refers to a type of lymphocyte that matures in the thymus. T cells play an important role in cell-mediated immunity and are distinguished from other lymphocytes, such as B cells, by the presence of a T-cell receptor on the cell surface. T-cells may either be isolated or obtained from a commercially available source. “T cell” includes all types of immune cells expressing CD3 including T-helper cells (CD4+ cells), cytotoxic T-cells (CD8+ cells), natural killer T-cells, T-regulatory cells (Treg), Tissue-resident memory T cells (Tim cells) and gamma-delta T cells. A “cytotoxic cell” includes CD8+ T cells, natural-killer (NK) cells, and neutrophils, which cells are capable of mediating cytotoxicity responses. Non-limiting examples of commercially available T-cell lines include lines BCL2 (AAA) Jurkat (ATCC® CRL-2902™), BCL2 (S70A) Jurkat (ATCC® CRL-2900™), BCL2 (S87A) Jurkat (ATCC® CRL-2901™), BCL2 Jurkat (ATCC® CRL-2899™), Neo Jurkat (ATCC® CRL-2898™), TALL-104 cytotoxic human T cell line (ATCC #CRL-11386). Further examples include but are not limited to mature T-cell lines, e.g., such as Deglis, EBT-8, HPB-MLp-W, HUT 78, HUT 102, Karpas 384, Ki 225, My-La, Se-Ax, SKW-3, SMZ-1 and T34; and immature T-cell lines, e.g., ALL-SIL, Be13, CCRF-CEM, CML-T1, DND-41, DU.528, EU-9, HD-Mar, HPB-ALL, H-SB2, HT-1, JK-T1, Jurkat, Karpas 45, KE-37, KOPT-K1, K-T1, L-KAW, Loucy, MAT, MOLT-1, MOLT 3, MOLT-4, MOLT 13, MOLT-16, MT-1, MT-ALL, P12/Ichikawa, Peer, PER0117, PER-255, PF-382, PFI-285, RPMI-8402, ST-4, SUP-T1 to T14, TALL-1, TALL-101, TALL-103/2, TALL-104, TALL-105, TALL-106, TALL-107, TALL-197, TK-6, TLBR-1, -2, -3, and -4, CCRF-HSB-2 (CCL-120.1), J.RT3-T3.5 (ATCC TIB-153), J45.01 (ATCC CRL-1990), J.CaM1.6 (ATCC CRL-2063), RS4;11 (ATCC CRL-1873), CCRF-CEM (ATCC CRM-CCL-119); and cutaneous T-cell lymphoma lines, e.g., HuT78 (ATCC CRM-TIB-161), MJ[G11] (ATCC CRL-8294), HuT102 (ATCC TIB-162). Null leukemia cell lines, including but not limited to REH, NALL-1, KM-3, L92-221, are a another commercially available source of immune cells, as are cell lines derived from other leukemias and lymphomas, such as K562 erythroleukemia, THP-1 monocytic leukemia, U937 lymphoma, HEL erythroleukemia, HL60 leukemia, HMC-1 leukemia, KG-1 leukemia, U266 myeloma. Non-limiting exemplary sources for such commercially available cell lines include the American Type Culture Collection, or ATCC, (http://www.atcc.org/) and the German Collection of Microorganisms and Cell Cultures (https://www.dsmz.de/).

As used herein, the term “engineered T-cell receptor” refers to a molecule comprising the elements of (a) an extracellular antigen binding domain, (b) a transmembrane domain, and (c) an intracellular signaling domain. In some aspect, an engineered T-cell receptor is a genetically modified TCR, a modified TCR, a recombinant TCR, a transgenic TCR, a partial TCR, a chimeric fusion protein, a CAR, a first generation CAR, a second generation CAR, a third generation CAR, or a fourth generation TRUCK. In some aspect, the engineered T-cell receptor comprises an antibody or a fragment of an antibody. In particular aspects, the engineered T-cell receptor is a genetically modified TCR or a CAR.

As used herein, the term “receptor” or “T-cell receptor” or “TCR” refers to a cell surface molecule found on T-cells that functions to recognize and bind antigens presented by antigen presenting molecules. Generally, a TCR is a heterodimer of an alpha chain (TRA) and a beta chain (TRB). Some TCRs are comprised of alternative gamma (TRG) and delta (TRD) chains. T-cells expressing this version of a TCR are known as γδ T-cells. TCRs are part of the immunoglobulin superfamily. Accordingly, like an antibody, the TCR comprises three hypervariable CDR regions per chain There is also an additional area of hypervariability on the beta-chain (HV4). The TCR heterodimer is generally present in an octomeric complex that further comprises three dimeric signaling modules CD3γ/ε, CD3δ/ε, and CD247 ζ/ζ or ζ/η. Non-limiting exemplary amino acid sequence of the human TCR-alpha chain: METLLGVSLVILWLQLARVNSQQGEEDPQALSIQEGENATMNCS YKTSINNLQWYRQNSGRGLVHLILIRSNEREKHSGRLRVTLDTSKKSSSLLITASRA A DTASYFCAPVLSGGGADGLTFGKGTHLIIQPYIQNPDPAVYQLRDSKSSDKSVCLFT D FDSQTNVSQSKDSDVYITDKTVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIP EDTFFPSPESSCDVKLVEKSFETDTNLNFQNLSVIGFRILLLKVAGFNLLMTLRLWSS

Non-limiting exemplary amino acid sequence of the human TCR-beta chain:

DSAVYLCASSLLRVYEQYFGPGTRLTVTEDLKNVFPPEVAVFEPPEAEI SHTQKATLVCLATGFYPDHVELSWWVNGKEVHSGVSTDPQPLKEQP.

The term “modified TCR” refers to a TCR that has been genetically engineered, and/or a transgenic TCR, and/or a recombinant TCR. Non-limiting examples of modified TCRs include single-chain VαVβ TCRs (scTv), full-length TCRs produced through use of a T cell display system, and TCRs wherein the CDR regions have been engineered to recognize a specific antigen, peptide, fragment, and/or MHC molecule. Methods of developing and engineering modified TCRs are known in the art. For example, see Stone, J. D. et al. Methods in Enzymology 503: 189-222 (2012), PCT Application WO2014018863 A1.

As used herein, the term “antibody” (“Ab”) collectively refers to immunoglobulins (or “Ig”) or immunoglobulin-like molecules including but not limited to antibodies of the following isotypes: IgM, IgA, IgD, IgE, IgG, and combinations thereof. Immunoglobulin-like molecules include but are not limited to similar molecules produced during an immune response in a vertebrate, for example, in mammals such as humans, rats, goats, rabbits and mice, as well as non-mammalian species, such as shark immunoglobulins (see Feige, M. et al. Proc. Nat. Ac. Sci. 41(22): 8155-60 (2014)). Unless specifically noted otherwise, the term “antibody” includes intact immunoglobulins and “antibody fragments” or “antigen binding fragments” that specifically bind to a molecule of interest (or a group of highly similar molecules of interest) to the substantial exclusion of binding to other molecules (for example, antibodies and antibody fragments that have a binding constant for the molecule of interest that is at least 103 M−1 greater, at least 104 M−1 greater or at least 105 M−1 greater than a binding constant for other molecules in a biological sample). The term “antibody” also includes genetically engineered forms such as chimeric antibodies (for example, humanized murine antibodies), heteroconjugate antibodies (such as, bispecific antibodies). See also, Pierce Catalog and Handbook, 1994-1995 (Pierce Chemical Co., Rockford, Ill.); Kuby, J., Immunology, 3rd Ed., W.H. Freeman & Co., New York, 1997.

As used herein, the term “monoclonal antibody” refers to an antibody produced by a cell into which the light and heavy chain genes of a single antibody have been transfected or, more traditionally, by a single clone of B-lymphocytes. Monoclonal antibodies generally have affinity for a single epitope (i.e. they are monovalent) but may be engineered to be specific for two or more epitopes (e.g. bispecific). Methods of producing monoclonal antibodies are known to those of skill in the art, for example by creating a hybridoma through fusion of myeloma cells with immune spleen cells, phage display, single cell amplification from B-cell populations, single plasma cell interrogation technologies, and single B-cell culture. Monoclonal antibodies include recombinant antibodies, chimeric antibodies, humanized antibodies, and human antibodies.

The general structure of an antibody is comprised of heavy (H) chains and light (L) chains connected by disulfide bonds. The structure can also comprise glycans attached at conserved amino acid residues. Each heavy and light chain contains a constant region and a variable region (also known as “domains”). There are two types of light chain, lambda (2) and kappa (κ). There are five primary types of heavy chains which determine the isotype (or class) of an antibody molecule: gamma (γ), delta (δ), alpha (α), mu (μ) and epsilon (ε). The constant regions of the heavy chain also contribute to the effector function of the antibody molecule. Antibodies comprising the heavy chains μ, δ, γ3, γ1, α1, γ2, γ4, ε, and α2 result in the following isotypes: IgM, IgD, IgG3, IgG1, IgA1, IgG2, IgG4, IgE, and IgA2, respectively. An IgY isotype, related to mammalian IgG, is found in reptiles and birds. An IgW isotype, related to mammalian IgD, is found in cartilaginous fish. Class switching is the process by which the constant region of an immunoglobulin heavy chain is replaced with a different immunoglobulin heavy chain through recombination of the heavy chain locus of a B-cell to produce an antibody of a different isotype. Antibodies may exist as monomers (e.g. IgG), dimers (e.g. IgA), tetramers (e.g. fish IgM), pentamers (e.g. mammalian IgM), and/or in complexes with other molecules. In some embodiments, antibodies can be bound to the surface of a cell or secreted by a cell.

The variable regions of the immunoglobulin heavy and the light chains specifically bind the antigen. The “framework” region is a portion of the Fab that acts as a scaffold for three hypervariable regions called “complementarity-determining regions” (CDRs). A set of CDRs is known as a paratope. The framework regions of different light or heavy chains are relatively conserved within a species. The combined framework region of an antibody (comprising regions from both light and heavy chains), largely adopts a β-sheet conformation and the CDRs form loops which connect, and in some cases form part of, the β-sheet structure. Thus, framework regions act to position the CDRs in correct orientation by inter-chain, non-covalent interactions. The framework region and CDRs for numerous antibodies have been defined and are available in a database maintained online (Kabat et al., Sequences of Proteins of Immunological Interest, U.S. Department of Health and Human Services, 1991).

The CDRs of the variable regions of heavy and light chains (VH and VL) are responsible for binding to an epitope of an antigen. A limited number of amino acid positions within the CDRs are directly involved in antigen binding. These positions within the CDRs are called specificity determining residues (SDRs). The CDRs of a heavy or light chain are numbered sequentially starting from the N-terminal end (i.e. CDR1, CDR2, and CDR3). For example, a VL CDR3 is the middle CDR located in the variable domain of the light chain of an antibody. A VH CDR1 is the first CDR in the variable domain of a heavy chain of an antibody. An antibody that binds a specific antigen will have specific VH and VL region sequences, and thus specific CDR sequences. Antibodies with different specificities (i.e. different combining sites for different antigens) have different CDRs.

The term “humanized” when used in reference to an antibody, means that the amino acid sequence of the antibody has non-human amino acid residues (e.g., mouse, rat, goat, rabbit, etc.) of one or more complementarity determining regions (CDRs) that specifically bind to the desired antigen in an acceptor human immunoglobulin molecule, and one or more human amino acid residues in the Fv framework region (FR), which are amino acid residues that flank the CDRs. Such antibodies typically have reduced immunogenicity and therefore a longer half-life in humans as compared to the non-human parent antibody from which one or more CDRs were obtained or are based upon.

An “antigen-binding fragment” (Fab) refers to the regions of an antibody corresponding to two of the three fragments produced by papain digestion. The Fab fragment comprises the region that binds to an antigen and is composed of one variable region and one constant region from both a heavy chain and a light chain. An F(ab′)2 fragment refers to a fragment of an antibody digested by pepsin or the enzyme IdeS (immunoglobulin degrading enzyme from S. pyogenes) comprising two Fab regions connected by disulfide bonds. A single chain variable fragment (“scFv”) refers to a fusion protein comprising at least one VH and at least one VL region connected by a linker of between 5 to 30 amino acids. Methods and techniques of developing scFv that bind to specific antigens are known in the art (see, e.g. Ahmad, Z. A. et al., Clinical and Developmental Immunology, 2012: 980250 (2012)).

As used herein, the term “antigen” refers to a compound, composition, or substance that may be specifically bound and/or recognized by the products of specific humoral or cellular immunity and antigen recognition molecules, including but not limited to an antibody molecule, single-chain variable fragment (scFv), cell surface immunoglobulin receptor, B-cell receptor (BCR), T-cell receptor (TCR), engineered TCR, modified TCR, or CAR. The term “epitope” refers to an antigen or a fragment, region, site, or domain of an antigen that is recognized by an antigen recognition molecule. Antigens can be any type of molecule including but not limited to peptides, proteins, lipids, phospholipids haptens, simple intermediary metabolites, sugars (e.g., monosaccharides or oligosaccharides), hormones, and macromolecules such as complex carbo-hydrates (e.g., polysaccharides). Some non-limiting examples of antigens include antigens involved in autoimmune disease (including autoantigens), allergy, and graft rejection, tumor antigens, toxins, and other miscellaneous antigens. Non-limiting examples of tumor antigens include mesothelin, ROR1 and EGFRvIII, ephrin type-A receptor 2 (EphA2), interleukin (IL)-13r alpha 2, an EGFR VIII, a PSMA, an EpCAM, a GD3, a fucosyl GM1, a PSCA, a PLAC1, a sarcoma breakpoint, a Wilms Tumor 1, a hematologic differentiation antigen, a surface glycoprotein, a gangliosides (GM2), a growth factor receptor, a stromal antigen, a vascular antigen, or a combination thereof. Antigens expressed by pathogens include, but are not limited to microbial antigens such as viral antigens, bacterial antigens, fungal antigens, protozoa, and other parasitic antigens.

As used herein, the term “target cell population” refers to a population of cells that present antigens, which can be targeted by engineered T cells. Non-limiting examples of target cell populations include tumor cells, cancer cells and pathogen infected cells. Non-limiting examples of pathogens include viral and bacterial pathogens.

As used herein, the term “antigen binding domain” refers to any protein or polypeptide domain that can specifically bind to an antigen target (including target complexes of antigens and MHC molecules).

As used herein, the term “autologous,” in reference to cells, tissue, and/or grafts refers to cells, tissue, and/or grafts that are isolated from and then and administered back into the same subject, patient, recipient, and/or host. “Allogeneic” refers to non-autologous cells, tissue, and/or grafts.

As used herein, the term “B cell,” refers to a type of lymphocyte in the humoral immunity of the adaptive immune system. B cells principally function to make antibodies, serve as antigen presenting cells, release cytokines, and develop memory B cells after activation by antigen interaction. B cells are distinguished from other lymphocytes, such as T cells, by the presence of a B-cell receptor on the cell surface. B cells may either be isolated or obtained from a commercially available source. Non-limiting examples of commercially available B cell lines include lines AHH-1 (ATCC® CRL-8146™), BC-1 (ATCC® CRL-2230™), BC-2 (ATCC® CRL-2231™), BC-3 (ATCC® CRL-2277™), CA46 (ATCC® CRL-1648™), DG-75 [D.G.-75] (ATCC® CRL-2625™), DS-1 (ATCC® CRL-11102™), EB-3 [EB3] (ATCC® CCL-85™), Z-138 (ATCC #CRL-3001), DB (ATCC CRL-2289), Toledo (ATCC CRL-2631), Pfiffer (ATCC CRL-2632), SR (ATCC CRL-2262), JM-1 (ATCC CRL-10421), NFS-5 C-1 (ATCC CRL-1693); NFS-70 C10 (ATCC CRL-1694), NFS-25 C-3 (ATCC CRL-1695), AND SUP-B15 (ATCC CRL-1929). Further examples include but are not limited to cell lines derived from anaplastic and large cell lymphomas, e.g., DEL, DL-40, FE-PD, JB6, Karpas 299, Ki-JK, Mac-2A Ply1, SR-786, SU-DHL-1, -2, -4, -5, -6, -7, -8, -9, -10, and -16, DOHH-2, NU-DHL-1, U-937, Granda 519, USC-DHL-1, RL; Hodgkin's lymphomas, e.g., DEV, HDLM-2, HD-MyZ, KM-H2, L 428, L 540, L1236, SBH-1, SUP-HD1, SU/RH-HD-1. Non-limiting exemplary sources for such commercially available cell lines include the American Type Culture Collection, or ATCC, (www.atcc.org/) and the German Collection of Microorganisms and Cell Cultures (https://www.dsmz.de/).

As used herein, the term “major histocompatibility complex” (MHC) refers to an antigen presentation molecule that functions as part of the immune system to bind antigens and other peptide fragments and display them on the cell surface for recognition by antigen recognition molecules such as TCR. MHC may be used interchangeably with the term “human leukocyte antigen” (HLA) when used in reference to human MHC; thus, MHC refers to all HLA subtypes including, but not limited to, the classical MHC genes disclosed herein: HLA-A, HLA-E, HLA-DM, HLA-DO, HLA-DP, HLA-DQ, and HLA-DR, in addition to all variants, isoforms, isotypes, and other biological equivalents thereof. MHC class I (MHC-I) and MHC class II (MHC-II) molecules utilize distinct antigen processing pathways. In general, peptides derived from intracellular antigens are presented to CD8+ T cells by MHC class I molecules, which are expressed on virtually all cells, while extracellular antigen-derived peptides are presented to CD4+ T cells by MHC-II molecules. However, several exceptions to this dichotomy have been observed. In certain embodiments disclosed herein, a particular antigen, peptide, and/or epitope is identified and presented in an antigen-MHC complex in the context of an appropriate MHC class I or II protein. The genetic makeup of a subject may be assessed to determine which MHC allele is suitable for a particular patient, disease, or condition with a particular set of antigens. In mice, the MHC genes are known as the histocompatibility 2 (H-2) genes. Murine classical MHC class I subtypes include H-2D, H-2K, and H-2L. Murine non-classical MHC class I subtypes include H-2Q, H-2M, and H-2T. Murine classical MHC class II subtypes include H-2A (I-A), and H-2E (1-E). Non-classical murine MHC class II subtypes include H-2M and H-20. Canine MHC molecules are known as Dog Leukocyte Antigens (DLA). Feline MHC molecules are known as Feline Leukocyte Antigens (FLA). In some embodiments, an orthologous or homologous MHC molecule is selected to transition a therapy or treatment involving a specific antigen-MHC complex from one species to a different species.

As used herein, a “target cell” is any cell that expresses the antigen target to which the engineered T cells can bind.

As used herein, a “cancer” is a disease state characterized by the presence in a subject of cells demonstrating abnormal uncontrolled replication and may be used interchangeably with the term “tumor.” In some embodiments, the cancer is a leukemia or a lymphoma. “Cell associated with the cancer” refers to those subject cells that demonstrate abnormal uncontrolled replication. In certain embodiments, the cancer is acute myeloid leukemia or acute lymphoblastic leukemia. As used herein a “leukemia” is a cancer of the blood or bone marrow characterized by an abnormal increase of immature white blood cells. The specific condition of acute myeloid leukemia (AML)—also referred to as acute myelogenous leukemia or acute myeloblastic leukemia—is a cancer of the myeloid origin blood cells, characterized by the rapid growth of abnormal myeloid cells that accumulate in the bone marrow and interfere with the production of normal blood cells. The specific condition of acute lymphoblastic leukemia (ALL)—also referred to as acute lymphocytic leukemia or acute lymphoid leukemia—is a cancer of the white blood cells, characterized by the overproduction and accumulation of malignant, immature leukocytes (lymphoblasts) resulting a lack of normal, healthy blood cells. As used herein a “lymphoma” is a cancer of the blood characterized by the development of blood cell tumors and symptoms of enlarged lymph nodes, fever, drenching sweats, unintended weight loss, itching, and constantly feeling tired.

A “solid tumor” is an abnormal mass of tissue that usually does not contain cysts or liquid areas. Solid tumors can be benign or malignant. Different types of solid tumors are named for the type of cells that form them. Examples of solid tumors include sarcomas, carcinomas, and lymphomas.

The term “B-cell lymphoma or leukemia” refers to a type of cancer that forms in issues of the lymphatic system or bone marrow and has undergone a malignant transformation that makes the cells within the cancer pathological to the host organism with the ability to invade or spread to other parts of the body.

One of skill in the art can monitor expression of genes using methods such as RNA-sequencing, DNA microarrays, Real-time PCR, or Chromatin immunoprecipitation (ChIP) etc. Protein expression can be monitored using methods such as flow cytometry, Western blotting, 2-D gel electrophoresis or immunoassays etc.

One of skill in the art can use methods such as RNA interference (RNAi), CRISPR, TALEN, ZFN or other methods that target specific sequences to reduce or eliminate expression and/or function of proteins. CRISPR, TALEN, ZFN or other genome editing tools can also be used to increase expression and/or function of genes.

As used herein, “RNAi” (RNA interference) refers to the method of reducing or eliminating gene expression in a cell by targeting specific mRNA sequences for degradation via introduction of short pieces of double stranded RNA (dsRNA) and small interfering RNA (such as siRNA, shRNA or miRNA etc.) (Agrawal, N. et al.; Microbiol Mol Biol Rev. 2003; 67:657-685, Arenz, C. et al.; Naturwissenschaften. 2003; 90:345-359, Hannon G J.; Nature. 2002; 418:244-251).

As used herein, the term “CRISPR” refers to a technique of sequence specific genetic manipulation relying on the clustered regularly interspaced short palindromic repeats pathway. CRISPR can be used to perform gene editing and/or gene regulation, as well as to simply target proteins to a specific genomic location. “Gene editing” refers to a type of genetic engineering in which the nucleotide sequence of a target polynucleotide is changed through introduction of deletions, insertions, single stranded or double stranded breaks, or base substitutions to the polynucleotide sequence. In some aspects, CRISPR-mediated gene editing utilizes the pathways of non-homologous end joining (NHEJ) or homologous recombination to perform the edits. Gene regulation refers to increasing or decreasing the production of specific gene products such as protein or RNA.

The term “gRNA” or “guide RNA” as used herein refers to guide RNA sequences used to target specific polynucleotide sequences for gene editing employing the CRISPR technique. Techniques of designing gRNAs and donor therapeutic polynucleotides for target specificity are well known in the art. For example, Doench, J., et al. Nature biotechnology 2014; 32(12):1262-7, Mohr, S. et al. (2016) FEBS Journal 283: 3232-38, and Graham, D., et al. Genome Biol. 2015; 16: 260. gRNA comprises or alternatively consists essentially of, or yet further consists of a fusion polynucleotide comprising CRISPR RNA (crRNA) and trans-activating CRIPSPR RNA (tracrRNA); or a polynucleotide comprising CRISPR RNA (crRNA) and trans-activating CRIPSPR RNA (tracrRNA). In some aspects, a gRNA is synthetic (Kelley, M. et al. (2016) J of Biotechnology 233 (2016) 74-83).

The term “Cas9” refers to a CRISPR associated endonuclease referred to by this name. Non-limiting exemplary Cas9s include Staphylococcus aureus Cas9, nuclease dead Cas9, and orthologs and biological equivalents each thereof. Orthologs include but are not limited to Streptococcus pyogenes Cas9 (“spCas9”), Cas 9 from Streptococcus thermophiles, Legionella pneumophilia, Neisseria lactamica, Neisseria meningitides, Francisella novicida; and Cpfl (which performs cutting functions analogous to Cas9) from various bacterial species including Acidaminococcus spp. and Francisella novicida U112.

As used herein, “TALEN” (transcription activator-like effector nucleases) refers to engineered nucleases that comprise a non-specific DNA-cleaving nuclease fused to a TALE DNA-binding domain, which can target DNA sequences and be used for genome editing. Boch (2011) Nature Biotech. 29: 135-6; and Boch et al. (2009) Science 326: 1509-12; Moscou et al. (2009) Science 326: 3501. TALEs are proteins secreted by Xanthomonas bacteria. The DNA binding domain contains a repeated, highly conserved 33-34 amino acid sequence, with the exception of the 12th and 13th amino acids. These two positions are highly variable, showing a strong correlation with specific nucleotide recognition. They can thus be engineered to bind to a desired DNA sequence. To produce a TALEN, a TALE protein is fused to a nuclease (N), which is a wild-type or mutated Fokl endonuclease. Several mutations to Fokl have been made for its use in TALENs; these, for example, improve cleavage specificity or activity. Cermak et al. (2011) Nucl. Acids Res. 39: e82; Miller et al. (2011) Nature Biotech. 29: 143-8; Hockemeyer et al. (2011) Nature Biotech. 29: 731-734; Wood et al. (2011) Science 333: 307; Doyon et al. (2010) Nature Methods 8: 74-79; Szczepek et al. (2007) Nature Biotech. 25: 786-793; and Guo et al. (2010) J. Mol. Bio. 200: 96. The Fokl domain functions as a dimer, requiring two constructs with unique DNA binding domains for sites in the target genome with proper orientation and spacing. Both the number of amino acid residues between the TALE DNA binding domain and the Fokl cleavage domain and the number of bases between the two individual TALEN binding sites appear to be important parameters for achieving high levels of activity. Miller et al. (2011) Nature Biotech. 29: 143-8. TALENs specific to sequences in immune cells can be constructed using any method known in the art, including various schemes using modular components. Zhang et al. (2011) Nature Biotech. 29: 149-53; Geibler et al. (2011) PLoS ONE 6: e 19509.

As used herein, “ZFN” (Zinc Finger Nuclease) refers to engineered nucleases that comprise a non-specific DNA-cleaving nuclease fused to a zinc finger DNA binding domain, which can target DNA sequences and be used for genome editing. Like a TALEN, a ZFN comprises a Fokl nuclease domain (or derivative thereof) fused to a DNA-binding domain. In the case of a ZFN, the DNA-binding domain comprises one or more zinc fingers. Carroll et al. (2011) Genetics Society of America 188: 773-782; and Kim et al. (1996) Proc. Natl. Acad. Sci. USA 93: 1156-1160. A zinc finger is a small protein structural motif stabilized by one or more zinc ions. A zinc finger can comprise, for example, Cys2His2, and can recognize an approximately 3-bp sequence. Various zinc fingers of known specificity can be combined to produce multi-finger polypeptides which recognize about 6, 9, 12, 15 or 18-bp sequences. Various selection and modular assembly techniques are available to generate zinc fingers (and combinations thereof) recognizing specific sequences, including phage display, yeast one-hybrid systems, bacterial one-hybrid and two-hybrid systems, and mammalian cells. Like a TALEN, a ZFN must dimerize to cleave DNA. Thus, a pair of ZFNs are required to target non-palindromic DNA sites. The two individual ZFNs must bind opposite strands of the DNA with their nucleases properly spaced apart. Bitinaite et al. (1998) Proc. Natl. Acad. Sci. USA 95: 10570-5. ZFNs specific to sequences in immune cells can be constructed using any method known in the art. See, e.g., Provasi (2011) Nature Med. 18: 807-815; Torikai (2013) Blood 122: 1341-1349; Cathomen et al. (2008) Mol. Ther. 16: 1200-7; Guo et al. (2010) J. Mol. Biol. 400: 96; U.S. Patent Publication 201110158957; and U.S. Patent Publication 2012/0060230.

A “cytotoxic cell” intends a cell that is capable of killing other cells or microbes. Examples of cytotoxic cells include but are not limited to CD8+ T cells, natural-killer (NK) cells, NKT cells, and neutrophils, which cells are capable of mediating cytotoxicity responses.

As used herein, the term “detectable marker” refers to at least one marker capable of directly or indirectly, producing a detectable signal. A non-exhaustive list of this marker includes enzymes which produce a detectable signal, for example by colorimetry, fluorescence, luminescence, such as horseradish peroxidase, alkaline phosphatase, (3-galactosidase, glucose-6-phosphate dehydrogenase, chromophores such as fluorescent, luminescent dyes, groups with electron density detected by electron microscopy or by their electrical property such as conductivity, amperometry, voltammetry, impedance, detectable groups, for example whose molecules are of sufficient size to induce detectable modifications in their physical and/or chemical properties, such detection may be accomplished by optical methods such as diffraction, surface plasmon resonance, surface variation, the contact angle change or physical methods such as atomic force spectroscopy, tunnel effect, or radioactive molecules such as ³²P, ³⁵S or ¹²⁵I.

As used herein, the term “purification marker” or “reporter protein” refer to at least one marker useful for purification or identification. A non-exhaustive list of this marker includes His, lacZ, GST, maltose-binding protein, NusA, BCCP, c-myc, CaM, FLAG, GFP, YFP, cherry, thioredoxin, poly(NANP), V5, Snap, HA, chitin-binding protein, Softag 1, Softag 3, Strep, or S-protein. Suitable direct or indirect fluorescence marker comprise FLAG, GFP, YFP, RFP, dTomato, cherry, Cy3, Cy 5, Cy 5.5, Cy 7, DNP, AMCA, Biotin, Digoxigenin, Tamra, Texas Red, rhodamine, Alexa fluors, FITC, TRITC or any other fluorescent dye or hapten.

As used herein, “homology” or “identical”, percent “identity” or “similarity”, when used in the context of two or more nucleic acids or polypeptide sequences, refers to two or more sequences or subsequences that are the same or have a specified percentage of nucleotides or amino acid residues that are the same, e.g., at least 60% identity, preferably at least 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or higher identity over a specified region (e.g., nucleotide sequence encoding the chimeric PVX described herein). Homology can be determined by comparing a position in each sequence which may be aligned for purposes of comparison. When a position in the compared sequence is occupied by the same base or amino acid, then the molecules are homologous at that position. A degree of homology between sequences is a function of the number of matching or homologous positions shared by the sequences. The alignment and the percent homology or sequence identity can be determined using software programs known in the art, for example those described in Current Protocols in Molecular Biology (Ausubel et al., eds. 1987) Supplement 30, section 7.7.18, Table 7.7.1. Preferably, default parameters are used for alignment. A preferred alignment program is BLAST, using default parameters. In particular, preferred programs are BLASTN and BLASTP, using the following default parameters: Genetic code=standard; filter=none; strand=both; cutoff=60; expect=10; Matrix=BLOSUM62; Descriptions=50 sequences; sort by=HIGH SCORE; Databases=non-redundant, GenBank+EMBL+DDBJ+PDB+GenBank CDS translations+SwissProtein+SPupdate+PIR. Details of these programs can be found at the following Internet address: ncbi.nlm.nih.gov/cgi-bin/BLAST. The terms “homology” or “identical,” percent “identity” or “similarity” also refer to, or can be applied to, the complement of a test sequence. The terms also include sequences that have deletions and/or additions, as well as those that have substitutions. As described herein, the preferred algorithms can account for gaps and the like. Preferably, identity exists over a region that is at least about 25 amino acids or nucleotides in length, or more preferably over a region that is at least 50-100 amino acids or nucleotides in length. An “unrelated” or “non-homologous” sequence shares less than 40% identity, or alternatively less than 25% identity, with one of the sequences disclosed herein.

The phrase “first line” or “second line” or “third line” refers to the order of treatment received by a patient. First line therapy regimens are treatments given first, whereas second or third line therapy are given after the first line therapy or after the second line therapy, respectively. The National Cancer Institute defines first line therapy as “the first treatment for a disease or condition. In patients with cancer, primary treatment can be surgery, chemotherapy, radiation therapy, or a combination of these therapies. First line therapy is also referred to those skilled in the art as “primary therapy and primary treatment.” See National Cancer Institute website at www.cancer.gov, last visited on May 1, 2008. Typically, a patient is given a subsequent chemotherapy regimen because the patient did not show a positive clinical or sub-clinical response to the first line therapy or the first line therapy has stopped.

It is to be inferred without explicit recitation and unless otherwise intended, that when the present disclosure relates to a polypeptide, protein, polynucleotide, an equivalent or a biologically equivalent of such is intended within the scope of this disclosure. As used herein, the term “biological equivalent thereof” is intended to be synonymous with “equivalent thereof” when referring to a reference protein, polypeptide or nucleic acid, intends those having minimal homology while still maintaining desired structure or functionality. Unless specifically recited herein, it is contemplated that any of the above also includes equivalents thereof. For example, an equivalent intends at least about 70% homology or identity, or at least 80% homology or identity and alternatively, or at least about 85%, or alternatively at least about 90%, or alternatively at least about 95%, or alternatively at least 98% percent homology or identity and/or exhibits substantially equivalent biological activity to the reference protein, polypeptide, or nucleic acid. Alternatively, when referring to polynucleotides, an equivalent thereof is a polynucleotide that hybridizes under stringent conditions to the reference polynucleotide or its complement.

The phrase “equivalent polypeptide” or “equivalent peptide fragment” refers to protein, polynucleotide, or peptide fragment encoded by a polynucleotide that hybridizes to a polynucleotide encoding the exemplified polypeptide or its complement of the polynucleotide encoding the exemplified polypeptide, under high stringency and/or which exhibit similar biological activity in vivo, e.g., approximately 100%, or alternatively, over 90% or alternatively over 85% or alternatively over 70%, as compared to the standard or control biological activity. Additional embodiments within the scope of this disclosure are identified by having more than 60%, or alternatively, more than 65%, or alternatively, more than 70%, or alternatively, more than 75%, or alternatively, more than 80%, or alternatively, more than 85%, or alternatively, more than 90%, or alternatively, more than 95%, or alternatively more than 97%, or alternatively, more than 98% or 99% sequence homology. Percentage homology can be determined by sequence comparison using programs such as BLAST run under appropriate conditions. In one aspect, the program is run under default parameters.

A polynucleotide or polynucleotide region (or a polypeptide or polypeptide region) having a certain percentage (for example, 80%, 85%, 90%, or 95%) of “sequence identity” to another sequence means that, when aligned, that percentage of bases (or amino acids) are the same in comparing the two sequences. The alignment and the percent homology or sequence identity can be determined using software programs known in the art, for example those described in Current Protocols in Molecular Biology (Ausubel et al., eds. 1987) Supplement 30, section 7.7.18, Table 7.7.1. Preferably, default parameters are used for alignment. A preferred alignment program is BLAST, using default parameters. In particular, preferred programs are BLASTN and BLASTP, using the following default parameters: Genetic code=standard; filter=none; strand=both; cutoff=60; expect=10; Matrix=BLOSUM62; Descriptions=50 sequences; sort by=HIGH SCORE; Databases=non-redundant, GenBank+EMBL+DDBJ+PDB+GenBank CDS translations+SwissProtein+SPupdate+PIR. Details of these programs can be found at the following Internet address: ncbi.nlm.nih.gov/cgi-bin/BLAST.

“Hybridization” refers to a reaction in which one or more polynucleotides react to form a complex that is stabilized via hydrogen bonding between the bases of the nucleotide residues. The hydrogen bonding may occur by Watson-Crick base pairing, Hoogstein binding, or in any other sequence-specific manner. The complex may comprise two strands forming a duplex structure, three or more strands forming a multi-stranded complex, a single self-hybridizing strand, or any combination of these. A hybridization reaction may constitute a step in a more extensive process, such as the initiation of a PCR reaction, or the enzymatic cleavage of a polynucleotide by a ribozyme.

Examples of stringent hybridization conditions include: incubation temperatures of about 25° C. to about 37° C.; hybridization buffer concentrations of about 6×SSC to about 10×SSC; formamide concentrations of about 0% to about 25%; and wash solutions from about 4×SSC to about 8×SSC. Examples of moderate hybridization conditions include: incubation temperatures of about 40° C. to about 50° C.; buffer concentrations of about 9×SSC to about 2×SSC; formamide concentrations of about 30% to about 50%; and wash solutions of about 5×SSC to about 2×SSC. Examples of high stringency conditions include: incubation temperatures of about 55° C. to about 68° C.; buffer concentrations of about 1×SSC to about 0.1×SSC; formamide concentrations of about 55% to about 75%; and wash solutions of about 1×SSC, 0.1×SSC, or deionized water. In general, hybridization incubation times are from 5 minutes to 24 hours, with 1, 2, or more washing steps, and wash incubation times are about 1, 2, or 15 minutes. SSC is 0.15 M NaCl and 15 mM citrate buffer. It is understood that equivalents of SSC using other buffer systems can be employed.

The term “isolated” as used herein refers to molecules or biologicals or cellular materials being substantially free from other materials. In one aspect, the term “isolated” refers to nucleic acid, such as DNA or RNA, or protein or polypeptide, or cell or cellular organelle, or tissue or organ, separated from other DNAs or RNAs, or proteins or polypeptides, or cells or cellular organelles, or tissues or organs, respectively, that are present in the natural source. The term “isolated” also refers to a nucleic acid or peptide that is substantially free of cellular material, viral material, or culture medium when produced by recombinant DNA techniques, or chemical precursors or other chemicals when chemically synthesized. Moreover, an “isolated nucleic acid” is meant to include nucleic acid fragments which are not naturally occurring as fragments and would not be found in the natural state. The term “isolated” is also used herein to refer to polypeptides which are isolated from other cellular proteins and is meant to encompass both purified and recombinant polypeptides. The term “isolated” is also used herein to refer to cells or tissues that are isolated from other cells or tissues and is meant to encompass both cultured and engineered cells or tissues.

The term “protein”, “peptide” and “polypeptide” are used interchangeably and in their broadest sense to refer to a compound of two or more subunit amino acids, amino acid analogs or peptidomimetics. The subunits may be linked by peptide bonds. In another aspect, the subunit may be linked by other bonds, e.g., ester, ether, etc. A protein or peptide must contain at least two amino acids and no limitation is placed on the maximum number of amino acids which may comprise a protein's or peptide's sequence. As used herein the term “amino acid” refers to either natural and/or unnatural or synthetic amino acids, including glycine and both the D and L optical isomers, amino acid analogs and peptidomimetics.

The terms “polynucleotide” and “oligonucleotide” are used interchangeably and refer to a polymeric form of nucleotides of any length, either deoxyribonucleotides or ribonucleotides or analogs thereof. Polynucleotides can have any three-dimensional structure and may perform any function, known or unknown. The following are non-limiting examples of polynucleotides: a gene or gene fragment (for example, a probe, primer, EST or SAGE tag), exons, introns, messenger RNA (mRNA), transfer RNA, ribosomal RNA, RNAi, ribozymes, cDNA, recombinant polynucleotides, branched polynucleotides, plasmids, vectors, isolated DNA of any sequence, isolated RNA of any sequence, nucleic acid probes and primers. A polynucleotide can comprise modified nucleotides, such as methylated nucleotides and nucleotide analogs. If present, modifications to the nucleotide structure can be imparted before or after assembly of the polynucleotide. The sequence of nucleotides can be interrupted by non-nucleotide components. A polynucleotide can be further modified after polymerization, such as by conjugation with a labeling component. The term also refers to both double- and single-stranded molecules. Unless otherwise specified or required, any aspect of this technology that is a polynucleotide encompasses both the double-stranded form and each of two complementary single-stranded forms known or predicted to make up the double-stranded form.

As used herein, the term “purified” does not require absolute purity; rather, it is intended as a relative term. Thus, for example, a purified nucleic acid, peptide, protein, biological complexes or other active compound is one that is isolated in whole or in part from proteins or other contaminants. Generally, substantially purified peptides, proteins, biological complexes, or other active compounds for use within the disclosure comprise more than 80% of all macromolecular species present in a preparation prior to admixture or formulation of the peptide, protein, biological complex or other active compound with a pharmaceutical carrier, excipient, buffer, absorption enhancing agent, stabilizer, preservative, adjuvant or other co-ingredient in a complete pharmaceutical formulation for therapeutic administration. More typically, the peptide, protein, biological complex or other active compound is purified to represent greater than 90%, often greater than 95% of all macromolecular species present in a purified preparation prior to admixture with other formulation ingredients. In other cases, the purified preparation may be essentially homogeneous, wherein other macromolecular species are not detectable by conventional techniques.

As used herein, the term “recombinant protein” refers to a polypeptide which is produced by recombinant DNA techniques, wherein generally, DNA encoding the polypeptide is inserted into a suitable expression vector which is in turn used to transform a host cell to produce the heterologous protein.

As used herein, “treating” or “treatment” of a disease in a subject refers to (1) preventing the symptoms or disease from occurring in a subject that is predisposed or does not yet display symptoms of the disease; (2) inhibiting the disease or arresting its development; or (3) ameliorating or causing regression of the disease or the symptoms of the disease. As understood in the art, “treatment” is an approach for obtaining beneficial or desired results, including clinical results. For the purposes of the present technology, beneficial or desired results can include one or more, but are not limited to, alleviation or amelioration of one or more symptoms, diminishment of extent of a condition (including a disease), stabilized (i.e., not worsening) state of a condition (including disease), delay or slowing of condition (including disease), progression, amelioration or palliation of the condition (including disease), states and remission (whether partial or total), whether detectable or undetectable. When the disease is cancer, the following clinical end points are non-limiting examples of treatment: reduction in tumor burden, slowing of tumor growth, longer overall survival, longer time to tumor progression, inhibition of metastasis or a reduction in metastasis of the tumor. In one aspect, treatment excludes prophylaxis.

As used herein, “anti-tumor immunity” in a subject refers to reducing or preventing the symptoms or cancer from occurring in a subject that is predisposed or does not yet display symptoms of the cancer.

In some embodiments a subject is in need of a treatment, cell or composition described herein. In certain embodiments a subject has or is suspected of having a neoplastic disorder, neoplasia, tumor, malignancy or cancer. In some embodiments a subject in need of a treatment, cell or composition described herein has or is suspected of having a neoplastic disorder, neoplasia, tumor, malignancy or cancer. In certain embodiments an engineered T cell described herein is used to treat a subject having, or suspected of having, a neoplastic disorder, neoplasia, tumor, malignancy or cancer.

In some embodiments, presented herein is a method of treating a subject having or suspected of having, a neoplasia, neoplastic disorder, tumor, cancer, or malignancy. In certain embodiments, a method of treating a subject comprises administering a therapeutically effective amount of an engineered T cell to a subject. In certain embodiments, a method comprises reducing or inhibiting proliferation of a neoplastic cell, tumor, cancer or malignant cell, comprising contacting the cell, tumor, cancer or malignant cell, with the engineered T cell in an amount sufficient to reduce or inhibit proliferation of the neoplastic cell, tumor, cancer or malignant cell.

In some embodiments, a method of reducing or inhibiting metastasis of a neoplasia, tumor, cancer or malignancy to other sites, or formation or establishment of metastatic neoplasia, tumor, cancer or malignancy at other sites distal from a primary neoplasia, tumor, cancer or malignancy, comprises administering to a subject an amount of an engineered T cell sufficient to reduce or inhibit metastasis of the neoplasia, tumor, cancer or malignancy to other sites, or formation or establishment of metastatic neoplasia, tumor, cancer or malignancy at other sites distal from the primary neoplasia, tumor, cancer or malignancy.

Non-limiting examples of a neoplasia, neoplastic disorder, tumor, cancer or malignancy include a carcinoma, sarcoma, neuroblastoma, cervical cancer, hepatocellular cancer, mesothelioma, glioblastoma, myeloma, lymphoma, leukemia, adenoma, adenocarcinoma, glioma, glioblastoma, retinoblastoma, astrocytoma, oligodendrocytoma, meningioma, or melanoma. A neoplasia, neoplastic disorder, tumor, cancer or malignancy may comprise or involve hematopoietic cells. Non-limiting examples of a sarcoma include a lymphosarcoma, liposarcoma, osteosarcoma, chondrosarcoma, leiomyosarcoma, rhabdomyosarcoma or fibrosarcoma. In some embodiments, a neoplasia, neoplastic disorder, tumor, cancer or malignancy is a myeloma, lymphoma or leukemia. In some embodiments, a neoplasia, neoplastic disorder, tumor, cancer or malignancy comprises a lung, thyroid, head or neck, nasopharynx, throat, nose or sinuses, brain, spine, breast, adrenal gland, pituitary gland, thyroid, lymph, gastrointestinal (mouth, esophagus, stomach, duodenum, ileum, jejunum (small intestine), colon, rectum), genito-urinary tract (uterus, ovary, cervix, endometrial, bladder, testicle, penis, prostate), kidney, pancreas, liver, bone, bone marrow, lymph, blood, muscle, or skin neoplasia, tumor, or cancer. In some embodiments, a neoplasia, neoplastic disorder, tumor, cancer or malignancy comprises a small cell lung or non-small cell lung cancer. In some embodiments, a neoplasia, neoplastic disorder, tumor, cancer or malignancy comprises a stem cell neoplasia, tumor, cancer or malignancy. In some embodiments, a neoplasia, neoplastic disorder, tumor, cancer or malignancy.

In some embodiments, a method inhibits, or reduces relapse or progression of the neoplasia, neoplastic disorder, tumor, cancer or malignancy. In some embodiments, a method comprises administering an anti-cell proliferative, anti-neoplastic, anti-tumor, anti-cancer or immune-enhancing treatment or therapy. In some embodiments, a method of treatment results in partial or complete destruction of the neoplastic, tumor, cancer or malignant cell mass; a reduction in volume, size or numbers of cells of the neoplastic, tumor, cancer or malignant cell mass; stimulating, inducing or increasing neoplastic, tumor, cancer or malignant cell necrosis, lysis or apoptosis; reducing neoplasia, tumor, cancer or malignancy cell mass; inhibiting or preventing progression or an increase in neoplasia, tumor, cancer or malignancy volume, mass, size or cell numbers; or prolonging lifespan. In some embodiments, a method of treatment results in reducing or decreasing severity, duration or frequency of an adverse symptom or complication associated with or caused by the neoplasia, tumor, cancer or malignancy. In some embodiments, a method of treatment results in reducing or decreasing pain, discomfort, nausea, weakness or lethargy. In some embodiments, a method of treatment results in increased energy, appetite, improved mobility or psychological well-being.

As used herein, the term “administer” and “administering” are used to mean introducing the therapeutic agent (e.g. polynucleotide, vector, cell, modified cell, population) into a subject. The therapeutic administration of this substance serves to attenuate any symptom, or prevent additional symptoms from arising. When administration is for the purposes of preventing or reducing the likelihood of developing an autoimmune disease or disorder, the substance is provided in advance of any visible or detectable symptom. Routes of administration include, but are not limited to, oral (such as a tablet, capsule or suspension), topical, transdermal, intranasal, vaginal, rectal, subcutaneous intravenous, intraarterial, intramuscular, intraosseous, intraperitoneal, epidural and intrathecal.

As used herein, the term “expression” refers to the process by which polynucleotides are transcribed into mRNA and/or the process by which the transcribed mRNA is subsequently being translated into peptides, polypeptides, or proteins. If the polynucleotide is derived from genomic DNA, expression may include splicing of the mRNA in a eukaryotic cell. The expression level of a gene may be determined by measuring the amount of mRNA or protein in a cell or tissue sample. In one aspect, the expression level of a gene from one sample may be directly compared to the expression level of that gene from a control or reference sample. In another aspect, the expression level of a gene from one sample may be directly compared to the expression level of that gene from the same sample following administration of a compound.

As used herein, the term “gene expression profile” refers to measuring the expression level of multiple genes to establish an expression profile for a particular sample.

As used herein, the term “lower than baseline expression” refers to reducing or eliminating the transcription of polynucleotides into mRNA, or alternatively reducing or eliminating the translation of mRNA into peptides, polypeptides, or proteins, or reducing or eliminating the functioning of peptides, polypeptides, or proteins. In a non-limiting example, the transcription of polynucleotides into mRNA is reduced to at least half of the normalized mean gene expression found in wild type cells.

As used herein, the term “higher than baseline expression” refers to increasing the transcription of polynucleotides into mRNA, or alternatively increasing the translation of mRNA into peptides, polypeptides, or proteins, or increasing the functioning of peptides, polypeptides, or proteins. In a non-limiting example, the transcription of polynucleotides into mRNA is increased to at least twice of the normalized mean gene expression found in wild type cells.

As used herein, the term “reduce or eliminate expression and/or function of” refers to reducing or eliminating the transcription of the polynucleotides into mRNA, or alternatively reducing or eliminating the translation of the mRNA into peptides, polypeptides, or proteins, or reducing or eliminating the functioning of the peptides, polypeptides, or proteins. In a non-limiting example, the transcription of polynucleotides into mRNA is reduced to at least half of its normal level found in wild type cells.

As used herein, the term “increase expression of” refers to increasing the transcription of the polynucleotides into mRNA, or alternatively increasing the translation of the mRNA into peptides, polypeptides, or proteins, or increasing the functioning of the peptides, polypeptides, or proteins. In a non-limiting example, the transcription of polynucleotides into mRNA is increased to at least twice of its normal level found in wild type cells.

As used herein, the term “overexpress” with respect to a cell, a tissue, or an organ expresses a protein to an amount that is greater than the amount that is produced in a control cell, a control issue, or an organ. A protein that is overexpressed may be endogenous to the host cell or exogenous to the host cell.

As used herein, the term “enhancer”, denotes sequence elements that augment, improve or ameliorate transcription of a nucleic acid sequence irrespective of its location and orientation in relation to the nucleic acid sequence to be expressed. An enhancer may enhance transcription from a single promoter or simultaneously from more than one promoter. As long as this functionality of improving transcription is retained or substantially retained (e.g., at least 70%, at least 80%, at least 90% or at least 95% of wild-type activity, that is, activity of a full-length sequence), any truncated, mutated or otherwise modified variants of a wild-type enhancer sequence are also within the above definition.

The term “promoter” as used herein refers to any sequence that regulates the expression of a coding sequence, such as a gene. Promoters may be constitutive, inducible, repressible, or tissue-specific, for example. A “promoter” is a control sequence that is a region of a polynucleotide sequence at which initiation and rate of transcription are controlled. It may contain genetic elements at which regulatory proteins and molecules may bind such as RNA polymerase and other transcription factors.

The term “contacting” means direct or indirect binding or interaction between two or more. A particular example of direct interaction is binding. A particular example of an indirect interaction is where one entity acts upon an intermediary molecule, which in turn acts upon the second referenced entity. Contacting as used herein includes in solution, in solid phase, in vitro, ex vivo, in a cell and in vivo. Contacting in vivo can be referred to as administering, or administration.

As used herein, the term “binds” or “antibody binding” or “specific binding” means the contact between the antigen binding domain of an antibody, antibody fragment, CAR, TCR, engineered TCR, BCR, MHC, immunoglobulin-like molecule, scFv, CDR or other antigen presentation molecule and an antigen, epitope, or peptide with a binding affinity (KD) of less than 10⁻⁵ M. In some aspects, an antigen binding domain binds to both a complex of both an antigen and an MHC molecule. In some aspects, antigen binding domains bind with affinities of less than about 10⁻⁶ M, 10⁻⁷M, and preferably 10⁻⁸ M, 10⁻⁹ M, 10⁻¹⁰ M, 10⁻¹¹M, or 10⁻¹² M. In a particular aspect, specific binding refers to the binding of an antigen to an MHC molecule, or the binding of an antigen binding domain of an engineered T-cell receptor to an antigen or antigen-MHC complex.

The term “introduce” as applied to methods of producing modified cells such as chimeric antigen receptor cells refers to the process whereby a foreign (i.e. extrinsic or extracellular) agent is introduced into a host cell thereby producing a cell comprising the foreign agent. Methods of introducing nucleic acids include but are not limited to transduction, retroviral gene transfer, transfection, electroporation, transformation, viral infection, and other recombinant DNA techniques known in the art. In some embodiments, transduction is done via a vector (e.g., a viral vector). In some embodiments, transfection is done via a chemical carrier, DNA/liposome complex, or micelle (e.g., Lipofectamine (Invitrogen)). In some embodiments, viral infection is done via infecting the cells with a viral particle comprising the polynucleotide of interest (e.g., AAV). In some embodiments, introduction further comprises CRISPR mediated gene editing or Transcription activator-like effector nuclease (TALEN) mediated gene editing. Methods of introducing non-nucleic acid foreign agents (e.g., soluble factors, cytokines, proteins, peptides, enzymes, growth factors, signaling molecules, small molecule inhibitors) include but are not limited to culturing the cells in the presence of the foreign agent, contacting the cells with the agent, contacting the cells with a composition comprising the agent and an excipient, and contacting the cells with vesicles or viral particles comprising the agent.

In the context of a nucleic acid or amino acid sequence, the term “chimeric” intends that the sequence contains is comprised of at least one substituent unit (e.g. fragment, region, portion, domain, polynucleotide, or polypeptide) that is derived from, obtained or isolated from, or based upon other distinct physical or chemical entities. For example, a chimera of two or more different proteins may comprise the sequence of a variable region domain from an antibody fused to the transmembrane domain of a cell signaling molecule. In some aspect, a chimera intends that the sequence is comprised of sequences from at least two distinct species.

The term “chimeric antigen receptor” (CAR), as used herein, refers to a fused protein comprising an extracellular domain capable of binding to an antigen, a transmembrane domain derived from a polypeptide different from a polypeptide from which the extracellular domain is derived, and at least one intracellular domain. The “chimeric antigen receptor (CAR)” is sometimes called a “chimeric receptor”, a “T-body”, or a “chimeric immune receptor (CIR).” The “extracellular domain capable of binding to an antigen” means any oligopeptide or polypeptide that can bind to a certain antigen. The “intracellular domain” or “intracellular signaling domain” means any oligopeptide or polypeptide known to function as a domain that transmits a signal to cause activation or inhibition of a biological process in a cell. In certain embodiments, the intracellular domain may comprise, alternatively consist essentially of, or yet further comprise one or more costimulatory signaling domains in addition to the primary signaling domain. The “transmembrane domain” means any oligopeptide or polypeptide known to span the cell membrane and that can function to link the extracellular and signaling domains. A chimeric antigen receptor may optionally comprise a “hinge domain” which serves as a linker between the extracellular and transmembrane domains. Non-limiting exemplary polynucleotide sequences that encode for components of each domain are disclosed herein, e.g.:

Hinge domain: IgG1 heavy chain hinge polynucleotide sequence:

CTCGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCG, and optionally an equivalent thereof.

Transmembrane domain: CD28 transmembrane region polynucleotide sequence:

TTTTGGGTGCTGGTGGTGGTTGGTGGAGTCCTGGCTTGCTATAGCTTGCT AGTAACAGTGGCCTTTATTATTTTCTGGGTG, and optionally an equivalent thereof.

Intracellular domain: 4-1BB co-stimulatory signaling region polynucleotide sequence:

AAACGGGGCAGAAAGAAACTCCTGTATATATTCAAACAACCATTTATGA GACCAGTACAAACTACTCAAGAGGAAGATGGCTGTAGCTGCCGATTTCCAGAA GAAGAAGAAGGAGGATGTGAACTG, and optionally an equivalent thereof.

Intracellular domain: CD28 co-stimulatory signaling region polynucleotide sequence:

AGGAGTAAGAGGAGCAGGCTCCTGCACAGTGACTACATGAACATGACTC CCCGCCGCCCCGGGCCCACCCGCAAGCATTACCAGCCCTATGCCCCACCACGCG ACTTCGCAGCCTATCGCTCC, and optionally an equivalent thereof.

Intracellular domain: CD3 zeta signaling region polynucleotide sequence:

AGAGTGAAGTTCAGCAGGAGCGCAGACGCCCCCGCGTACCAGCAGGGC CAGAACCAGCTCTATAACGAGCTCAATCTAGGACGAAGAGAGGAGTACGATGT TTTGGACAAGAGACGTGGCCGGGACCCTGAGATGGGGGGAAAGCCGAGAAGG AAGAACCCTCAGGAAGGCCTGTACAATGAACTGCAGAAAGATAAGATGGCGGA GGCCTACAGTGAGATTGGGATGAAAGGCGAGCGCCGGAGGGGCAAGGGGCAC GATGGCCTTTACCAGGGTCTCAGTACAGCCACCAAGGACACCTACGACGCCCTT CACATGCAGGCCCTGCCCCCTCGCTAA, and optionally an equivalent thereof.

Non-limiting examples of CAR extracellular domains capable of binding to antigens are the anti-CD19 binding domain sequences that specifically bind CD19 antigen as disclosed in the US20140271635 application.

Further embodiments of each exemplary domain component include other proteins that have analogous biological function that share at least 70%, or alternatively at least 80% amino acid sequence identity, preferably 90% sequence identity, more preferably at least 95% sequence identity with the proteins encoded by the above disclosed nucleic acid sequences. Further, non-limiting examples of such domains are provided herein.

As used herein, the term “CD8α hinge domain” refers to a specific protein fragment associated with this name and any other molecules that have analogous biological function that share at least 70%, or alternatively at least 80% amino acid sequence identity, preferably 90% sequence identity, more preferably at least 95% sequence identity with the CD8 α hinge domain sequence as shown herein. The example sequences of CD8 α hinge domain for human, mouse, and other species are provided in Pinto, R. D. et al. (2006) Vet. Immunol. Immunopathol. 110:169-177. The sequences associated with the CD8 α hinge domain are provided in Pinto, R. D. et al. (2006) Vet. Immunol. Immunopathol. 110:169-177. Non-limiting examples of such include:

Human CD8 alpha hinge domain amino acid sequence: PAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACDIY, and optionally an equivalent thereof.

Mouse CD8 alpha hinge domain amino acid sequence: KVNSTTTKPVLRTPSPVHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIY, and optionally an equivalent thereof.

Cat CD8 alpha hinge domain amino acid sequence: PVKPTTTPAPRPPTQAPITTSQRVSLRPGTCQPSAGSTVEASGLDLSCDIY, and optionally an equivalent thereof.

As used herein, the term “CD8 α transmembrane domain” refers to a specific protein fragment associated with this name and any other molecules that have analogous biological function that share at least 70%, or alternatively at least 80% amino acid sequence identity, preferably 90% sequence identity, more preferably at least 95% sequence identity with the CD8 α transmembrane domain sequence as shown herein. The fragment sequences associated with the amino acid positions 183 to 203 of the human T-cell surface glycoprotein CD8 alpha chain (GenBank Accession No: NP_001759.3), or the amino acid positions 197 to 217 of the mouse T-cell surface glycoprotein CD8 alpha chain (GenBank Accession No: NP_001074579.1), and the amino acid positions 190 to 210 of the rat T-cell surface glycoprotein CD8 alpha chain (GenBank Accession No: NP_113726.1) provide additional example sequences of the CD8 α transmembrane domain. The sequences associated with each of the listed accession numbers are provided as follows:

Human CD8 alpha transmembrane domain amino acid sequence: IYIWAPLAGTCGVLLLSLVIT, and optionally an equivalent thereof.

Mouse CD8 alpha transmembrane domain amino acid sequence: IWAPLAGICVALLLSLIITLI, and optionally an equivalent thereof.

Rat CD8 alpha transmembrane domain amino acid sequence: IWAPLAGICAVLLLSLVITLI, and optionally an equivalent thereof.

As used herein, the term “CD28 transmembrane domain” refers to a specific protein fragment associated with this name and any other molecules that have analogous biological function that share at least 70%, or alternatively at least 80% amino acid sequence identity, at least 90% sequence identity, or alternatively at least 95% sequence identity with the CD28 transmembrane domain sequence as shown herein. The fragment sequences associated with the GenBank Accession Nos: XM_006712862.2 and XM_009444056.1 provide additional, non-limiting, example sequences of the CD28 transmembrane domain.

As used herein, the term “4-1BB costimulatory signaling region” refers to a specific protein fragment associated with this name and any other molecules that have analogous biological function that share at least 70%, or alternatively at least 80% amino acid sequence identity, preferably 90% sequence identity, more preferably at least 95% sequence identity with the 4-1BB costimulatory signaling region sequence as shown herein. Non-limiting example sequences of the 4-1BB costimulatory signaling region are provided in U.S. Publication 20130266551A1 (filed as U.S. application Ser. No. 13/826,258), such as the exemplary sequence provided below and the sequence encoded by 4-1BB costimulatory signaling region amino acid sequence: KRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCEL, and optionally an equivalent thereof.

As used herein, the term “ICOS costimulatory signaling region” refers to a specific protein fragment associated with this name and any other molecules that have analogous biological function that share at least 70%, or alternatively at least 80% amino acid sequence identity, preferably 90% sequence identity, more preferably at least 95% sequence identity with the ICOS costimulatory signaling region sequence as shown herein. Non-limiting example sequences of the ICOS costimulatory signaling region are provided in U.S. Patent Application Publication No. 2015/0017141A1 the exemplary polynucleotide sequence provided below.

ICOS costimulatory signaling region polynucleotide sequence: ACAAAAAAGA AGTATTCATC CAGTGTGCAC GACCCTAACG GTGAATACAT GTTCATGAGA GCAGTGAACA CAGCCAAAAA ATCCAGACTC ACAGATGTGA CCCTA, and optionally an equivalent thereof.

As used herein, the term “OX40 costimulatory signaling region” refers to a specific protein fragment associated with this name and any other molecules that have analogous biological function that share at least 70%, or alternatively at least 80% amino acid sequence identity, or alternatively 90% sequence identity, or alternatively at least 95% sequence identity with the OX40 costimulatory signaling region sequence as shown herein. Non-limiting example sequences of the OX40 costimulatory signaling region are disclosed in U.S. Patent Application Publication No. 2012/20148552A1, and include the exemplary sequence provided below.

OX40 costimulatory signaling region polynucleotide sequence:

AGGGACCAG AGGCTGCCCC CCGATGCCCA CAAGCCCCCT GGGGGAGGCA GTTTCCGGAC CCCCATCCAA GAGGAGCAGG CCGACGCCCA CTCCACCCTG GCCAAGATC, and optionally an equivalent thereof.

As used herein, the term “CD28 costimulatory signaling region” refers to a specific protein fragment associated with this name and any other molecules that have analogous biological function that share at least 70%, or alternatively at least 80% amino acid sequence identity, or alternatively 90% sequence identity, or alternatively at least 95% sequence identity with the CD28 costimulatory signaling region sequence shown herein. The example sequences CD28 costimulatory signaling domain are provided in U.S. Pat. No. 5,686,281; Geiger, T. L. et al. (2001) Blood 98: 2364-2371; Hombach, A. et al. (2001) J Immunol 167: 6123-6131; Maher, J. et al. (2002) Nat Biotechnol 20: 70-75; Haynes, N. M. et al. (2002) J Immunol. 169: 5780-5786 (2002); Haynes, N. M. et al. (2002) Blood 100: 3155-3163. A non-limiting example include the sequence encoded by:

CD28 amino acid sequence: MLRLLLALNL FPSIQVTGNK ILVKQSPMLV AYDNAVNLSC KYSYNLFSRE FRASLHKGLDSAVEVCVVYG NYSQQLQVYS KTGFNCDGKL GNESVTFYLQ NLYVNQTDIY FCKIEVMYPPPYLDNEKSNG TIIHVKGKHL CPSPLFPGPS KPFWVLVVVG GVLACYSLLVTVAFIIFWVR SKRSRLLHSD YMNMTPRRPG PTRKHYQPYA PPRDFAAYRS, and equivalents thereof.

As used herein, the term “CD3 zeta signaling domain” refers to a specific protein fragment associated with this name and any other molecules that have analogous biological function that share at least 70%, or alternatively at least 80% amino acid sequence identity, or alternatively 90% sequence identity, or alternatively at least 95% sequence identity with the CD3 zeta signaling domain sequence as shown herein. Non-limiting example sequences of the CD3 zeta signaling domain amino acid sequence are provided in U.S. application Ser. No. 13/826,258, e.g.:

RVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKP RRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATK DTYDALHMQALPPR.

As used herein, a “first generation CAR” refers to a CAR comprising an extracellular domain capable of binding to an antigen, a transmembrane domain derived from a polypeptide different from a polypeptide from which the extracellular domain is derived, and at least one intracellular domain. A “second generation CAR” refers to a first generation CAR further comprising one costimulation domain (e.g. 4-1BB or CD28). A “third generation CAR” refers to a first generation CAR further comprising two costimulation domains (e.g. CD27, CD28, ICOS, 4-1BB, or OX40). A “fourth generation CAR” (also known as a “TRUCK”) refers to a CAR T-cell further engineered to secrete an additional factor (e.g. proinflammatory cytokine IL-12). A review of these CAR technologies and cell therapy is found in Maus, M. et al. Clin. Cancer Res. 22(3): 1875-84 (2016).

As used herein, the term “suicide gene” is a gene capable of inducing cell apoptosis; non-limiting examples include HSV-TK (Herpes simplex virus thymidine kinase), cytosine deaminase, nitroreductase, carboxylesterase, cytochrome P450 or PNP (Purine nucleoside phosphorylase), truncated EGFR, or inducible caspase (“iCasp”). Suicide genes may function along a variety of pathways, and, in some cases, may be inducible by an inducing agent such as a small molecule. For example, the iCasp suicide gene comprises portion of a caspase protein operatively linked to a protein optimized to bind to an inducing agent; introduction of the inducing agent into a cell comprising the suicide gene results in the activation of caspase and the subsequent apoptosis of the cell.

The term “transduce” or “transduction” as it is applied to the production of chimeric antigen receptor cells refers to the process whereby a foreign nucleotide sequence is introduced into a cell. In some embodiments, this transduction is done via a vector.

As used herein, the term “vector” refers to a nucleic acid construct deigned for transfer between different hosts, including but not limited to a plasmid, a virus, a cosmid, a phage, a BAC, a YAC, etc. A “viral vector” is defined as a recombinantly produced virus or viral particle that comprises a polynucleotide to be delivered into a host cell, either in vivo, ex vivo or in vitro. In some embodiments, plasmid vectors may be prepared from commercially available vectors. In other embodiments, viral vectors may be produced from baculoviruses, retroviruses, adenoviruses, AAVs, etc. according to techniques known in the art. In one embodiment, the viral vector is a lentiviral vector. Examples of viral vectors include retroviral vectors, adenovirus vectors, adeno-associated virus vectors, alphavirus vectors and the like. Infectious tobacco mosaic virus (TMV)-based vectors can be used to manufacturer proteins and have been reported to express Griffithsin in tobacco leaves (O'Keefe et al. (2009) Proc. Nat. Acad. Sci. USA 106(15):6099-6104). Alphavirus vectors, such as Semliki Forest virus-based vectors and Sindbis virus-based vectors, have also been developed for use in gene therapy and immunotherapy. See, Schlesinger & Dubensky (1999) Curr. Opin. Biotechnol. 5:434-439 and Ying et al. (1999) Nat. Med. 5(7):823-827. In aspects where gene transfer is mediated by a retroviral vector, a vector construct refers to the polynucleotide comprising the retroviral genome or part thereof, and a gene of interest such as a polynucleotide encoding a CAR. Further details as to modern methods of vectors for use in gene transfer may be found in, for example, Kotterman et al. (2015) Viral Vectors for Gene Therapy: Translational and Clinical Outlook Annual Review of Biomedical Engineering 17. Vectors that contain both a promoter and a cloning site into which a polynucleotide can be operatively linked are well known in the art. Such vectors are capable of transcribing RNA in vitro or in vivo, and are commercially available from sources such as Agilent Technologies (Santa Clara, Calif.) and Promega Biotech (Madison, Wis.).

As used herein, the terms “T2A” and “2A peptide” are used interchangeably to refer to any 2A peptide or fragment thereof, any 2A-like peptide or fragment thereof, or an artificial peptide comprising the requisite amino acids in a relatively short peptide sequence (on the order of 20 amino acids long depending on the virus of origin) containing the consensus polypeptide motif D-V/I-E-X-N-P-G-P, wherein X refers to any amino acid generally thought to be self-cleaving.

As used herein, the term “recombinant protein” refers to a polypeptide which is produced by recombinant DNA techniques, wherein generally, DNA encoding the polypeptide is inserted into a suitable expression vector which is in turn used to transform a host cell to produce the heterologous protein.

As used herein, the term “signal peptide” or “signal polypeptide” intends an amino acid sequence usually present at the N-terminal end of newly synthesized secretory or membrane polypeptides or proteins. It acts to direct the polypeptide across or into a cell membrane and is then subsequently removed. Examples of such are well known in the art. Non-limiting examples are those described in U.S. Pat. Nos. 8,853,381 and 5,958,736.

As used herein in reference to a regulatory polynucleotide, the term “operatively linked” refers to an association between the regulatory polynucleotide and the polynucleotide sequence to which it is linked such that, when a specific protein binds to the regulatory polynucleotide, the linked polynucleotide is transcribed.

The term “culturing” refers to growing cells in a culture medium under conditions that favor expansion and proliferation of the cell. The term “culture medium” or “medium” is recognized in the art and refers generally to any substance or preparation used for the cultivation of living cells. The term “medium”, as used in reference to a cell culture, includes the components of the environment surrounding the cells. Media may be solid, liquid, gaseous or a mixture of phases and materials. Media include liquid growth media as well as liquid media that do not sustain cell growth. Media also include gelatinous media such as agar, agarose, gelatin and collagen matrices. Exemplary gaseous media include the gaseous phase to which cells growing on a petri dish or other solid or semisolid support are exposed. The term “medium” also refers to material that is intended for use in a cell culture, even if it has not yet been contacted with cells. In other words, a nutrient rich liquid prepared for culture is a medium. Similarly, a powder mixture that when mixed with water or other liquid becomes suitable for cell culture may be termed a “powdered medium.” “Defined medium” refers to media that are made of chemically defined (usually purified) components. “Defined media” do not contain poorly characterized biological extracts such as yeast extract and beef broth. “Rich medium” includes media that are designed to support growth of most or all viable forms of a particular species. Rich media often include complex biological extracts. A “medium suitable for growth of a high-density culture” is any medium that allows a cell culture to reach an OD600 of 3 or greater when other conditions (such as temperature and oxygen transfer rate) permit such growth. The term “basal medium” refers to a medium which promotes the growth of many types of microorganisms which do not require any special nutrient supplements. Most basal media generally comprise of four basic chemical groups: amino acids, carbohydrates, inorganic salts, and vitamins. A basal medium generally serves as the basis for a more complex medium, to which supplements such as serum, buffers, growth factors, lipids, and the like are added. In one aspect, the growth medium may be a complex medium with the necessary growth factors to support the growth and expansion of the cells of the disclosure while maintaining their self-renewal capability. Examples of basal media include, but are not limited to, Eagles Basal Medium, Minimum Essential Medium, Dulbecco's Modified Eagle's Medium, Medium 199, Nutrient Mixtures Ham's F-10 and Ham's F-12, McCoy's 5A, Dulbecco's MEM/F-I 2, RPMI 1640, and Iscove's Modified Dulbecco's Medium (IMDM).

“Cryoprotectants” are known in the art and include without limitation, e.g., sucrose, trehalose, and glycerol. A cryoprotectant exhibiting low toxicity in biological systems is generally used.

Modes of Carrying Out the Disclosure Modified T-Cells and Methods of Producing the Same

Disclosed herein are modified T-cells modified to exhibit higher than or lower than baseline expression of one or more genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7, or to express a T-cell receptor comprising, or consisting essentially of, or yet further consisting of at least one of the amino acid sequences set forth in Table 6. The one or more gene may be selected from the group of 4-1BB, PD-1, CD103 or TIM3. In one aspect, the baseline expression is normalized mean gene expression. In another aspect, the higher than baseline expression is at least about a 2-fold increase in expression relative to baseline expression and/or lower than baseline expression is at least about a 2-fold decrease in expression relative to baseline expression. Expression can be reduced or increased by at least about 2 or more, or about 3, or about 4, or about 5, or about 6, or about 7, or about 8, or about 9, or about 10, or about 11, or about 12, or about 13, or about 14, or about 15 fold as compared to a comparative wild-type cell. One of skill in the art can monitor expression of the genes using methods such as RNA-sequencing, DNA microarrays, Real-time PCR, or Chromatin immunoprecipitation (ChIP) etc. Protein expression can be monitored using methods such as flow cytometry, Western blotting, 2-D gel electrophoresis or immunoassays etc.

In a further aspect, the T-cells are tissue-resident memory cells (T_(RM)), CD8+ T-cells or tumor-infiltrating lymphocytes (TILs). In certain other aspects, the T-cells and/or TRMs are CD19−CD20−CD14−CD56−CD4−CD45+CD3+CD8 cells. In certain aspects, the T-cells and/or TRMs are TRMs expressing high levels of TIM3, CXCL13 and CD39. In one particular embodiment, the T-cells are autologous to the subject being treated.

The modified T-cell may be genetically modified, optionally using gene editing technologies, e.g., recombinant methods, CRISPR/Cas system, ZFN, and/or TALEN. Aspects of the present disclosure relate to an isolated cell comprising, or alternatively consisting essentially of, or yet further consisting of a CAR of this disclosure and methods of producing such cells. The T-cell or NK cell can be from any preferred species, e.g., an animal cell, a mammalian cell such as a human, a feline or a canine cell.

In some aspect of the present disclosure, the population of isolated cells transduced with the nucleic acid sequence encoding the CAR as described herein is a population of NK precursor cells and/or T-cell precursor cells. Transduction of precursor cells results in a long-lived population of cells capable of differentiating into CAR T-cells and/or CAR NK cells. T-cell precursors include but are not limited to HSCs; long term HSCs; MPPs; CLPs; LMPPs/ELPs; DN1s; DN2s; DN3s; DN4s; DPs. NK precursors include but are not limited to HSCs, long term HSCs, MPPs, CMPs, GMPs, pro-NK, pre-NK, and iNK cells. In a specific aspect, the population of isolated cells includes both mature T-cells and T-cell precursors to provide both short lived effector CAR T-cells and long-lived CAR T-cell precursors for transplant into the subject. In another aspect, the population of isolated cells includes both mature NK cells and NK precursors to provide both short lived effector CAR NK cells and long-lived CAR NK precursors for transplant into the subject.

In specific embodiments, the isolated cell comprises, or alternatively consists essentially of, or yet further consists of an exogenous CAR comprising, or alternatively consisting essentially of, or yet further consisting of, an antigen binding domain of the antibody provided herein, a CD8 α hinge domain, a CD8 α transmembrane domain, a CD28 costimulatory signaling region and/or a 4-1BB costimulatory signaling region, and a CD3 zeta signaling domain. In certain embodiments, the isolated cell is a T-cell, e.g., an animal T-cell, a mammalian T-cell, a feline T-cell, a canine T-cell or a human T-cell. In certain embodiments, the isolated cell is an NK-cell, e.g., an animal NK-cell, a mammalian NK-cell, a feline NK-cell, a canine NK-cell or a human NK-cell.

In some embodiments, T-cells expressing the disclosed CARs may be further modified to reduce or eliminate expression of endogenous TCRs. Reduction or elimination of endogenous TCRs can reduce off-target effects and increase the effectiveness of the T cells. T cells stably lacking expression of a functional TCR may be produced using a variety of approaches. T cells internalize, sort, and degrade the entire T cell receptor as a complex, with a half-life of about 10 hours in resting T cells and 3 hours in stimulated T cells (von Essen, M. et al. 2004. J. Immunol. 173:384-393). Proper functioning of the TCR complex requires the proper stoichiometric ratio of the proteins that compose the TCR complex. TCR function also requires two functioning TCR zeta proteins with ITAM motifs. The activation of the TCR upon engagement of its MHC-peptide ligand requires the engagement of several TCRs on the same T cell, which all must signal properly. Thus, if a TCR complex is destabilized with proteins that do not associate properly or cannot signal optimally, the T cell will not become activated sufficiently to begin a cellular response.

Accordingly, in some embodiments, TCR expression may eliminated using RNA interference (e.g., shRNA, siRNA, miRNA, etc.), CRISPR, or other methods that target the nucleic acids encoding specific TCRs (e.g., TCR-α and TCR-β) and/or CD3 chains in primary T cells. By blocking expression of one or more of these proteins, the T cell will no longer produce one or more of the key components of the TCR complex, thereby destabilizing the TCR complex and preventing cell surface expression of a functional TCR. Even though some TCR complexes can be recycled to the cell surface when RNA interference is used, the RNA (e.g., shRNA, siRNA, miRNA, etc.) will prevent new production of TCR proteins resulting in degradation and removal of the entire TCR complex, resulting in the production of a T cell having a stable deficiency in functional TCR expression.

Expression of inhibitory RNAs (e.g., shRNA, siRNA, miRNA, etc.) in primary T cells can be achieved using any conventional expression system, e.g., a lentiviral expression system. Although lentiviruses are useful for targeting resting primary T cells, not all T cells will express the shRNAs. Some of these T cells may not express sufficient amounts of the RNAs to allow enough inhibition of TCR expression to alter the functional activity of the T cell. Thus, T cells that retain moderate to high TCR expression after viral transduction can be removed, e.g., by cell sorting or separation techniques, so that the remaining T cells are deficient in cell surface TCR or CD3, enabling the expansion of an isolated population of T cells deficient in expression of functional TCR or CD3.

Expression of CRISPR in primary T cells can be achieved using conventional CRISPR/Cas systems and guide RNAs specific to the target TCRs. Suitable expression systems, e.g. lentiviral or adenoviral expression systems are known in the art. Similar to the delivery of inhibitor RNAs, the CRISPR system can be used to specifically target resting primary T cells or other suitable immune cells for CAR cell therapy. Further, to the extent that CRISPR editing is unsuccessful, cells can be selected for success according to the methods disclosed above. For example, as noted above, T cells that retain moderate to high TCR expression after viral transduction can be removed, e.g., by cell sorting or separation techniques, so that the remaining T cells are deficient in cell surface TCR or CD3, enabling the expansion of an isolated population of T cells deficient in expression of functional TCR or CD3. It is further appreciated that a CRISPR editing construct may be useful in both knocking out the endogenous TCR and knocking in the CAR constructs disclosed herein. Accordingly, it is appreciated that a CRISPR system can be designed for to accomplish one or both of these purposes.

Sources of Isolated Cells: Prior to expansion and genetic modification of the cells disclosed herein, cells may be obtained from a subject—for instance, in embodiments involving autologous therapy—or a commercially available culture, that are available from the American Type Culture Collection (ATCC), for example.

Cells can be obtained from a number of sources in a subject, including peripheral blood mononuclear cells, bone marrow, lymph node tissue, cord blood, thymus tissue, tissue from a site of infection, ascites, pleural effusion, spleen tissue, and tumors.

Methods of isolating relevant cells are well known in the art and can be readily adapted to the present application; an exemplary method is described in the examples below. Isolation methods for use in relation to this disclosure include but are not limited to Life Technologies Dynabeads® system; STEMcell Technologies EasySep™, RoboSep™ RosetteSep™, SepMate™; Miltenyi Biotec MACS™ cell separation kits, and other commercially available cell separation and isolation kits. Particular subpopulations of immune cells and precursors may be isolated through the use of fluorescence-activated cell sorting (FACS), beads, or other binding agents available in such kits specific to unique cell surface markers. For example, MACS™ CD4+ and CD8+ MicroBeads may be used to isolate CD4+ and CD8+ T-cells.

Alternatively, cells may be obtained through commercially available cell cultures, including but not limited to, for T-cells, lines BCL2 (AAA) Jurkat (ATCC® CRL-2902™), BCL2 (S70A) Jurkat (ATCC® CRL-2900™), BCL2 (S87A) Jurkat (ATCC® CRL-2901™), BCL2 Jurkat (ATCC® CRL-2899™), Neo Jurkat (ATCC® CRL-2898™); and, for NK cells, lines NK-92 (ATCC® CRL-2407™), NK-92MI (ATCC® CRL-2408™).

In some aspect, the subject may be administered a conditioning regimen to induce precursor cell mobilization into the peripheral blood prior to obtaining the cells from the subject. For example, a subject may be administered an effective amount of at least one of granulocyte colony-stimulating factor (G-CSF), filgrastim (Neupogen), sargramostim (Leukine), pegfilgrastim (Neulasta), and mozobil (Plerixafor) up to two weeks prior to or concurrently with isolation of cells from the subject. Mobilized precursor cells can be obtained from the subject by any method known in the art, including, for example, leukapheresis 1-14 days following administration of the conditioning regimen.

Activation and Expansion of T Cells: Whether prior to or after genetic modification of the T cells to express a desirable CAR, the cells can be activated and expanded using generally known methods such as those described in U.S. Pat. Nos. 6,352,694; 6,534,055; 6,905,680; 6,692,964; 5,858,358; 6,887,466; 6,905,681; 7,144,575; 7,067,318; 7,172,869; 7,232,566; 7,175,843; 5,883,223; 6,905,874; 6,797,514; 6,867,041. Methods of activating relevant cells are well known in the art and can be readily adapted to the present application; an exemplary method is described in the examples below. Isolation methods for use in relation to this disclosure include but are not limited to Life Technologies Dynabeads® system activation and expansion kits; BD Biosciences Phosflow™ activation kits, Miltenyi Biotec MACS™ activation/expansion kits, and other commercially available cell kits specific to activation moieties of the relevant cell. Particular subpopulations of immune cells may be activated or expanded through the use of beads or other agents available in such kits. For example, α-CD3/α-CD28 Dynabeads® may be used to activate and expand a population of isolated T-cells.

Also disclosed herein is an isolated cell comprising, or alternatively consisting essentially of, or yet further consisting of the CAR of this disclosure.

The modified T-cell disclosed herein can also be further modified to express a protein that binds to a cytokine, chemokine, lymphokine, or a receptor each thereof. In one aspect, the protein comprises, or consists essentially of, or yet further consists of an antibody or an antigen binding fragment thereof.

In another aspect, the antibody is an IgG, IgA, IgM, IgE or IgD, or a subclass thereof. The antibody can also be an IgG selected from the group of IgG1, IgG2, IgG3 or IgG4. Furthermore, the antigen binding fragment can be selected from the group of a Fab, Fab′, F(ab′)2, Fv, Fd, single-chain Fvs (scFv), disulfide-linked Fvs (sdFv) or VL or VH.

In one aspect, the modified T-cell of this disclosure comprises, or consists essentially of, or yet further consists of a chimeric antigen receptor (CAR). In one embodiment, the chimeric antigen receptor (CAR) comprises, or consists essentially of, or yet further consists of: (a) an antigen binding domain; (b) a hinge domain; (c) a transmembrane domain; (d) and an intracellular domain.

Spacer Domain: The CARs may optionally further comprise, or alternatively consist essentially of, or yet further consist of a spacer domain of up to 300 amino acids, preferably 10 to 100 amino acids, more preferably 25 to 50 amino acids. For example, the spacer may be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 amino acids. A spacer domain may comprise, for example, a portion of a human Fc domain, a CH₃ domain, or the hinge region of any immunoglobulin, such as IgA, IgD, IgE, IgG, or IgM, or variants thereof. For example, some embodiments may comprise an IgG₄ hinge with or without a S228P, L235E, and/or N297Q mutation (according to Kabat numbering). Additional spacers include, but are not limited to, CD4, CD8, and CD28 hinge regions.

Transmembrane Domain. The transmembrane domain may be derived either from a natural or from a synthetic source. Where the source is natural, the domain may be derived from any membrane-bound or transmembrane protein. Transmembrane regions of particular use in this disclosure may be derived from CD8, CD28, CD3, CD45, CD4, CD5, CDS, CD9, CD 16, CD22, CD33, CD37, CD64, CD80, CD86, CD 134, CD137, CD 154, TCR. Alternatively, the transmembrane domain may be synthetic, in which case it will comprise predominantly hydrophobic residues such as leucine and valine. Preferably a triplet of phenylalanine, tryptophan and valine will be found at each end of a synthetic transmembrane domain. Optionally, a short oligo- or polypeptide linker, preferably between 2 and 10 amino acids in length may form the linkage between the transmembrane domain and the cytoplasmic signaling domain of the CAR. A glycine-serine doublet provides a particularly suitable linker.

Cytoplasmic Domain. The cytoplasmic domain or intracellular signaling domain of the CAR is responsible for activation of at least one of the traditional effector functions of an immune cell in which a CAR has been placed. The intracellular signaling domain refers to a portion of a protein which transduces the effector function signal and directs the immune cell to perform its specific function. An entire signaling domain or a truncated portion thereof may be used so long as the truncated portion is sufficient to transduce the effector function signal. Cytoplasmic sequences of the T-cell receptor (TCR) and co-receptors, as well as derivatives or variants thereof, can function as intracellular signaling domains for use in a CAR. Intracellular signaling domains of particular use in this disclosure may be derived from FcR, TCR, CD3, CDS, CD22, CD79a, CD79b, CD66d. In some embodiments, the signaling domain of the CAR comprises, or consists essentially thereof, or consists of a CD3 ζ signaling domain.

Co-stimulatory Domains. Since signals generated through the TCR are alone insufficient for full activation of a T cell, a secondary or co-stimulatory signal may also be required. Thus, the intracellular region of at least one co-stimulatory signaling molecule, including but not limited to CD27, CD28, 4-1BB (CD 137), OX40, CD30, CD40, PD-1, ICOS, lymphocyte function-associated antigen-1 (LFA-1), CD2, CD7, LIGHT, NKG2C, B7-H3, or a ligand that specifically binds with CD83, may also be included in the cytoplasmic domain of the CAR. CARs of the present disclosure can comprise, or consist essentially thereof, or consist of one or more co-stimulatory domain. For instance, a CAR may comprise, or consist essentially thereof, or consist of one, two, or more co-stimulatory domains, in addition to a signaling domain (e.g., a CD3 signaling domain).

In some embodiments, the cell activation moiety of the chimeric antigen receptor is a T-cell signaling domain comprising, or alternatively consisting essentially of, or yet further consisting of, one or more proteins or fragments thereof selected from the group consisting of CD8 protein, CD28 protein, 4-1BB protein, OX40, CD30, CD40, PD-1, ICOS, LFA-1, CD2, CD7, CD27, LIGHT, NKG2C, B7-H3 and CD3-zeta protein.

In specific embodiments, the CAR comprises, or alternatively consists essentially thereof, or yet consists of an antigen binding domain of an any of the antibodies of this disclosure or fragment (e.g., scFv) thereof, a CD8 α or an IgG1 hinge domain, a CD8 α transmembrane domain, at least one costimulatory signaling region, and a CD3 zeta signaling domain. In further embodiments, the costimulatory signaling region comprises, or alternatively consists essentially thereof, or yet consists of either or both a CD28 costimulatory signaling region and a 4-1BB costimulatory signaling region.

In one embodiment, the antigen binding domain comprises, or consists essentially of, or yet further consists of an anti-CD19 antigen binding domain, the transmembrane domain comprises, or consists essentially of, or yet further consists of a CD28, CD28H (TMIGD2), AMICA1 or a CD8 α transmembrane domain and the one or more costimulatory regions selected from a CD28 costimulatory signaling region, a 4-1BB costimulatory signaling region, an ICOS costimulatory signaling region, an AMICA1 costimulatory signaling region, a CD28H (TMIGD2) costimulatory signaling region, and an OX40 costimulatory region or a CD3 zeta signaling domain. In a further embodiment, the anti-CD19 binding domain comprises, or consists essentially of, or yet further consists of a single-chain variable fragment (scFv) that specifically recognizes a humanized anti-CD19 binding domain. The anti-CD19 binding domain scFv of the CAR may comprise, or consist essentially of, or yet further consist of a heavy chain variable region and a light chain variable region.

In one aspect, the anti-CD19 binding domain of the CAR further comprises, or consists essentially of, or yet further consists of a linker polypeptide located between the anti-CD19 binding domain scFv heavy chain variable region and the anti-CD19 binding domain scFv light chain variable region. The linker polypeptide of the CAR may comprise, or consist essentially of, or yet further consist of a polypeptide of the sequence (GGGGS)n wherein n is an integer from 1 to 6. The linker peptide may be from 1 to 50 amino acids, for instance, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 amino acids. In some embodiments, the linker is glycine rich, although it may also contain serine or threonine. In another aspect, the CAR can further comprise, or consist essentially of, or yet further consist of a detectable marker attached to the CAR. In a separate aspect, the CAR can further comprise, or consist essentially of, or yet further consist of a purification marker attached to the CAR.

Switch Mechanisms. In some embodiments, the CAR may also comprise, or consist essentially thereof, or consist of a switch mechanism for controlling expression and/or activation of the CAR. For example, a CAR may comprise, consist, or consist essentially of an extracellular, transmembrane, and intracellular domain, in which the extracellular domain comprises a target-specific binding element that comprises a label, binding domain, or tag that is specific for a molecule other than the target antigen that is expressed on or by a target cell. In such embodiments, the specificity of the CAR is provided by a second construct that comprises, consists, or consists essentially of a target antigen binding domain and a domain that is recognized by or binds to the label, binding domain, or tag on the CAR. See, e.g., WO 2013/044225, WO 2016/000304, WO 2015/057834, WO 2015/057852, WO 2016/070061, U.S. Pat. No. 9,233,125, US 2016/0129109. In this way, a T-cell that expresses the CAR can be administered to a subject, but it cannot bind its target antigen until the second composition comprising a specific binding domain is administered.

CARs of the present disclosure may likewise require multimerization in order to activate their signaling function (see, e.g., US 2015/0368342, US 2016/0175359, US 2015/0368360) and/or an exogenous signal, such as a small molecule drug (US 2016/0166613, Yung et al., Science, 2015) in order to elicit a T-cell response.

Furthermore, the disclosed CARs can comprise, or consist essentially thereof, or consist of a “suicide switch” to induce cell death of the CAR T-cells following treatment (Buddee et al., PLoS One, 2013) or to downregulate expression of the CAR following binding to the target antigen (WO 2016/011210).

Also provided herein are modified T-cells prepared by any of the methods disclosed below. Further provided herein is a substantially homogenous population of cells of any of the modified T-cells of this disclosure. Also provided herein is a heterogeneous population of cells of any of the modified T-cells of this disclosure.

In one aspect, the method of producing the modified T-cells comprises, or alternatively consists essentially of, or yet further consists of isolating the T-cells and culturing the cells under conditions that favor expansion and proliferation of the cells. The modified T-cell may be genetically modified, optionally using recombinant methods, CRISPR/Cas system, ZFN, and/or TALEN.

CARs may be prepared using vectors. Aspects of the present disclosure relate to an isolated nucleic acid sequence encoding the CARs disclosed herein and vectors comprising, or alternatively consisting essentially of, or yet further consisting of an isolated nucleic acid sequence encoding the CAR and its complement and equivalents of each thereof.

The CAR cells of this disclosure can be generated by inserting into the modified T-cell a polynucleotide encoding the CAR and then expressing the CAR in the cell, Thus, in one aspect, the engineered T cell of this disclosure comprises, or alternatively consists essentially of, or yet further consists of a polynucleotide encoding the CAR, wherein the polynucleotide further comprises, or alternatively consists essentially of, or yet further consists of a promoter operatively linked to the polynucleotide to express the polynucleotide in the cell. Non-limiting examples of promoters include constitutive, inducible, repressible, or tissue-specific. The promoter is “operatively linked” in a manner to transcribe the linked polynucleotide.

Further provided herein is a modified T-cell comprising, or consisting essentially of, or yet further consisting of a polynucleotide encoding the CAR, and optionally, wherein the polynucleotide encodes and anti-CD19 binding domain. In one aspect, the polynucleotide may further comprise, or consist essentially of, or yet further consist of a promoter operatively linked to the polynucleotide to express the polynucleotide in the modified T-cell. In another aspect, the polynucleotide may further comprise, or consist essentially of, or yet further consist of a 2A self-cleaving peptide (T2A) encoding polynucleotide sequence located upstream of a polynucleotide encoding the anti-CD19 binding domain. “T2A” and “2A peptide” are used interchangeably to refer to any 2A peptide or fragment thereof, any 2A-like peptide or fragment thereof, or an artificial peptide comprising the requisite amino acids in a relatively short peptide sequence (on the order of 20 amino acids long depending on the virus of origin) containing the consensus polypeptide motif D-V/I-E-X-N-P-G-P, wherein X refers to any amino acid generally thought to be self-cleaving.

In yet a further aspect, the polynucleotide may further comprise, or consist essentially of, or yet further consist of a polynucleotide encoding a signal peptide located upstream of a polynucleotide encoding the anti-CD19 binding domain. In some embodiments, the polynucleotide comprises, or alternatively consists essentially thereof, or yet further consists of, a Kozak consensus sequence upstream of the polynucleotide sequence encoding the antigen binding domain or an enhancer. In some embodiments, the polynucleotide comprises, or alternatively consists essentially thereof, or yet further consists of a polynucleotide conferring antibiotic resistance. In one particular embodiment, the isolated nucleic acid encoding the CAR further comprises, or alternatively consists essentially thereof, or yet further consists of a switch mechanism for controlling expression and/or activation of the CAR.

The preparation of exemplary vectors and the generation of CAR expressing cells using the vectors is discussed in detail in the examples below. In summary, the expression of natural or synthetic nucleic acids encoding CARs is typically achieved by operably linking a nucleic acid encoding the CAR polypeptide or portions thereof to a promoter and incorporating the construct into an expression vector. The vectors can be suitable for replication and integration eukaryotes. Methods for producing cells comprising vectors and/or exogenous nucleic acids are well-known in the art. See, for example, Sambrook et al. (2001, Molecular Cloning A Laboratory Manual, Cold Spring Harbor Laboratory, New York).

In several aspects, the vector is derived from or based on a wild-type virus. In further aspects, the vector is derived from or based on a wild-type lentivirus. Examples of such, include without limitation, human immunodeficiency virus (HIV), equine infectious anemia virus (EIAV), simian immunodeficiency virus (SW) and feline immunodeficiency virus (FIV). Alternatively, it is contemplated that other retrovirus can be used as a basis for a vector backbone such murine leukemia virus (MLV). It will be evident that a viral vector according to the disclosure need not be confined to the components of a particular virus. The viral vector may comprise components derived from two or more different viruses and may also comprise synthetic components. Vector components can be manipulated to obtain desired characteristics, such as target cell specificity.

The recombinant vectors of this disclosure may be derived from primates and non-primates. Examples of primate lentiviruses include the human immunodeficiency virus (HIV), the causative agent of human acquired immunodeficiency syndrome (AIDS), and the simian immunodeficiency virus (SIV). The non-primate lentiviral group includes the prototype “slow virus” visna/maedi virus (VMV), as well as the related caprine arthritis-encephalitis virus (CAEV), equine infectious anemia virus (EIAV) and the more recently described feline immunodeficiency virus (FIV) and bovine immunodeficiency virus (BIV). Prior art recombinant lentiviral vectors are known in the art, e.g., see U.S. Pat. Nos. 6,924,123; 7,056,699; 7,07,993; 7,419,829 and 7,442,551, incorporated herein by reference.

U.S. Pat. No. 6,924,123 discloses that certain retroviral sequence facilitate integration into the target cell genome. This patent teaches that each retroviral genome comprises genes called gag, pol and env which code for virion proteins and enzymes. These genes are flanked at both ends by regions called long terminal repeats (LTRs). The LTRs are responsible for proviral integration, and transcription. They also serve as enhancer-promoter sequences. In other words, the LTRs can control the expression of the viral genes. Encapsidation of the retroviral RNAs occurs by virtue of a psi sequence located at the 5′ end of the viral genome. The LTRs themselves are identical sequences that can be divided into three elements, which are called U3, R and U5. U3 is derived from the sequence unique to the 3′ end of the RNA. R is derived from a sequence repeated at both ends of the RNA, and U5 is derived from the sequence unique to the 5′end of the RNA. The sizes of the three elements can vary considerably among different retroviruses. For the viral genome. and the site of poly (A) addition (termination) is at the boundary between R and U5 in the right-hand side LTR. U3 contains most of the transcriptional control elements of the provirus, which include the promoter and multiple enhancer sequences responsive to cellular and in some cases, viral transcriptional activator proteins.

With regard to the structural genes gag, pol and env themselves, gag encodes the internal structural protein of the virus. Gag protein is proteolytically processed into the mature proteins MA (matrix), CA (capsid) and NC (nucleocapsid). The pol gene encodes the reverse transcriptase (RT), which contains DNA polymerase, associated RNase H and integrase (IN), which mediate replication of the genome.

For the production of viral vector particles, the vector RNA genome is expressed from a DNA construct encoding it, in a host cell. The components of the particles not encoded by the vector genome are provided in trans by additional nucleic acid sequences (the “packaging system”, which usually includes either or both of the gag/pol and env genes) expressed in the host cell. The set of sequences required for the production of the viral vector particles may be introduced into the host cell by transient transfection, or they may be integrated into the host cell genome, or they may be provided in a mixture of ways. The techniques involved are known to those skilled in the art.

Retroviral vectors for use in this disclosure include but are not limited to Invitrogen's pLenti series versions 4, 6, and 6.2 “ViraPower” system. Manufactured by Lentigen Corp.; pHIV-7-GFP, lab generated and used by the City of Hope Research Institute; “Lenti-X” lentiviral vector, pLVX, manufactured by Clontech; pLKO.1-puro, manufactured by Sigma-Aldrich; pLemiR, manufactured by Open Biosystems; and pLV, lab generated and used by Charité Medical School, Institute of Virology (CBF), Berlin, Germany.

Regardless of the method used to introduce exogenous nucleic acids into a host cell or otherwise expose a cell to the inhibitor of the present disclosure, in order to confirm the presence of the recombinant DNA sequence in the host cell, a variety of assays may be performed. Such assays include, for example, “molecular biological” assays well known to those of skill in the art, such as Southern and Northern blotting, RT-PCR and PCR; “biochemical” assays, such as detecting the presence or absence of a particular peptide, e.g., by immunological means (ELISAs and Western blots) or by assays described herein to identify agents falling within the scope of the disclosure.

Packaging vector and cell lines: CARs can be packaged into a lentiviral or retroviral packaging system by using a packaging vector and cell lines. The packaging plasmid includes, but is not limited to retroviral vector, lentiviral vector, adenoviral vector, and adeno-associated viral vector. The packaging vector contains elements and sequences that facilitate the delivery of genetic materials into cells. For example, the retroviral constructs are packaging plasmids comprising at least one retroviral helper DNA sequence derived from a replication-incompetent retroviral genome encoding in trans all virion proteins required to package a replication incompetent retroviral vector, and for producing virion proteins capable of packaging the replication-incompetent retroviral vector at high titer, without the production of replication-competent helper virus. The retroviral DNA sequence lacks the region encoding the native enhancer and/or promoter of the viral 5′ LTR of the virus, and lacks both the psi function sequence responsible for packaging helper genome and the 3′ LTR, but encodes a foreign polyadenylation site, for example the SV40 polyadenylation site, and a foreign enhancer and/or promoter which directs efficient transcription in a cell type where virus production is desired. The retrovirus is a leukemia virus such as a Moloney Murine Leukemia Virus (MMLV), the Human Immunodeficiency Virus (HIV), or the Gibbon Ape Leukemia virus (GALV). The foreign enhancer and promoter may be the human cytomegalovirus (HCMV) immediate early (IE) enhancer and promoter, the enhancer and promoter (U3 region) of the Moloney Murine Sarcoma Virus (MMSV), the U3 region of Rous Sarcoma Virus (RSV), the U3 region of Spleen Focus Forming Virus (SFFV), or the HCMV IE enhancer joined to the native Moloney Murine Leukemia Virus (MMLV) promoter. The retroviral packaging plasmid may consist of two retroviral helper DNA sequences encoded by plasmid-based expression vectors, for example where a first helper sequence contains a cDNA encoding the gag and pol proteins of ecotropic MMLV or GALV and a second helper sequence contains a cDNA encoding the env protein. The Env gene, which determines the host range, may be derived from the genes encoding xenotropic, amphotropic, ecotropic, polytropic (mink focus forming) or 10A1 murine leukemia virus env proteins, or the Gibbon Ape Leukemia Virus (GALV env protein, the Human Immunodeficiency Virus env (gp160) protein, the Vesicular Stomatitus Virus (VSV) G protein, the Human T cell leukemia (HTLV) type I and II env gene products, chimeric envelope gene derived from combinations of one or more of the aforementioned env genes or chimeric envelope genes encoding the cytoplasmic and transmembrane of the aforementioned env gene products and a monoclonal antibody directed against a specific surface molecule on a desired target cell.

In the packaging process, the packaging plasmids and retroviral vectors are transiently co-transfected into a first population of mammalian cells that are capable of producing virus, such as human embryonic kidney cells, for example 293 cells (ATCC No. CRL1573, ATCC, Rockville, Md.), to produce high titer recombinant retrovirus-containing supernatants. In another method of the disclosure this transiently transfected first population of cells is then co-cultivated with mammalian target cells, for example human lymphocytes, to transduce the target cells with the foreign gene at high efficiencies. In yet another method of the disclosure the supernatants from the above described transiently transfected first population of cells are incubated with mammalian target cells, for example human lymphocytes or hematopoietic stem cells, to transduce the target cells with the foreign gene at high efficiencies.

In another aspect, the packaging plasmids are stably expressed in a first population of mammalian cells that are capable of producing virus, such as human embryonic kidney cells, for example 293 cells. Retroviral or lentiviral vectors are introduced into cells by either co-transfection with a selectable marker or infection with pseudotyped virus. In both cases, the vectors integrate. Alternatively, vectors can be introduced in an episomally maintained plasmid. High titer recombinant retrovirus-containing supernatants are produced.

In one embodiment, the polynucleotide further comprises, or consists essentially of, or yet further consists of a vector. In one particular embodiment, the vector is a plasmid. In another embodiment, the vector is a viral vector selected from the group of a retroviral vector, a lentiviral vector, an adenoviral vector, and an adeno-associated viral vector.

In some embodiments, the T cell of this disclosure has been isolated from a subject. In a particular embodiment, the T cell of this disclosure has been isolated from a subject, wherein the subject has cancer. In one aspect, the cancer or tumor is an epithelial, a head, neck, lung, lung, prostate, colon, pancreas, esophagus, liver, skin, kidney, adrenal gland, brain, or comprises a lymphoma, breast, endometrium, uterus, ovary, testes, lung, prostate, colon, pancreas, esophagus, liver, skin, kidney, adrenal gland and/or brain cancer or tumor, a metastasis or recurring tumor, cancer or neoplasia, a non-small cell lung cancer (NSCLC) and/or head and neck squamous cell cancer (HNSCC). In another aspect the subject is The term “subject,” “host,” “individual,” and “patient” are as used interchangeably herein to refer to animals, typically mammalian animals. Any suitable mammal can be treated by a method, cell or composition described herein. Non-limiting examples of mammals include humans, non-human primates (e.g., apes, gibbons, chimpanzees, orangutans, monkeys, macaques, and the like), domestic animals (e.g., dogs and cats), farm animals (e.g., horses, cows, goats, sheep, pigs) and experimental animals (e.g., mouse, rat, rabbit, guinea pig). In some embodiments a mammal is a human. A mammal can be any age or at any stage of development (e.g., an adult, teen, child, infant, or a mammal in utero). A mammal can be male or female. A mammal can be a pregnant female. In some embodiments a subject is a human. In some embodiments, a subject has or is suspected of having a cancer or neoplastic disorder.

Compositions, Methods of Treatment, Diagnosis and Prognosis

Also disclosed herein is a composition comprising, or consisting essentially of, or yet further consisting of a population of modified T-cells described above. Further provided herein is a composition comprising, or alternatively consisting essentially of, or yet further consisting of a carrier and one or more of: the modified T cell of this disclosure and/or the population of modified T-cells of this disclosure. In one aspect, the population is a substantially homogenous cell population. In another aspect, the population is a heterogeneous population. The composition of the present disclosure also can be bound to many different carriers. Examples of well-known carriers include glass, polystyrene, polypropylene, polyethylene, dextran, nylon, amylases, natural and modified celluloses, polyacrylamides, agaroses and magnetite. The nature of the carrier can be either soluble or insoluble for purposes of the disclosure. Those skilled in the art will know of other suitable carriers, or will be able to ascertain such, using routine experimentation.

Further provided herein are methods to identify the antigens or antigen receptors associated with the isolated and/or purified cell populations disclosed herein. In some aspect, the receptors are T-cell receptors (TCRs). In particular embodiments, the TCRs comprise the sequences listed in Table 6. In certain embodiments, the identified antigens or antigen receptors can be used for example to vaccinate a subject against cancer or an immune response. In other aspects, the identified antigens or antigen receptors can be used to engineer cells, for example a chimeric-antigen receptor T-cell (CAR-T cell). In still other aspects, the engineered CAR-T cell can be used to provide immunotherapy to a subject such as for example, a human patient. Also provided herein are methods to induce an immune response and treat conditions requiring selective immunotherapy, comprising, or consisting essentially of, or yet further consisting of, contacting a target cell with the cells or compositions as described herein. The contacting can be performed in vitro, or alternatively in vivo, thereby providing immunotherapy to a subject such as for example, a human patient.

Provided herein are methods to identify the antigens or antigen receptors associated with the isolated and/or purified cell populations disclosed herein. In some aspect, the receptors are T-cell receptors (TCRs). In particular embodiments, the TCRs comprise the sequences listed in Table 6. In certain embodiments, the identified antigens or antigen receptors can be used for example to vaccinate a subject against cancer or an immune response. In other aspects, the identified antigens or antigen receptors can be used to engineer cells, for example a chimeric-antigen receptor T-cell (CAR-T cell). In still other aspects, the engineered CAR-T cell can be used to provide immunotherapy to a subject such as for example, a human patient.

Also provided herein are methods to induce an immune response and treat conditions requiring selective immunotherapy, comprising, or consisting essentially of, or yet further consisting of, contacting a target cell with the cells or compositions as described herein.

Provided herein is a method of treating cancer, providing anti-tumor immunity, preventing relapse of cancer, and/or eliciting an anti-tumor response in a subject comprising, or consisting essentially of, or yet further consisting of administering to the subject an effective amount of a population of T-cells that exhibit higher than or lower than baseline expression of one or more genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7, or that express a T-cell receptor comprising at least one of the amino acid sequences set forth in Table 6. Providing anti-tumor immunity refers to preventing the symptoms or cancer from occurring in a subject that is predisposed or does not yet display symptoms of the cancer. In another aspect, it is to inhibit relapse or progression of cancer in a subject in need thereof.

In one aspect, the method comprises, or consists essentially of, or yet further consists of administering to the subject an effective amount of an agent that induces higher than or lower than baseline expression of one or more genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 in T-cells, or a T-cell receptor comprising at least one of the amino acid sequences set forth in Table 6. In another aspect, the method comprises, or consists essentially of, or yet further consists of administering an effective amount of one or more an agent that induces or inhibits in T-cells activity of one or more proteins encoded by genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 to the subject or sample. The active agent can be an antibody, a small molecule, a protein, a peptide, a ligand mimetic or a nucleic acid. The one or more gene may be selected from the group of 4-1BB, PD-1, CD103 or TIM3. In one aspect, the baseline expression is normalized mean gene expression. In another aspect, the higher than baseline expression is at least about a 2-fold increase in expression relative to baseline expression and/or lower than baseline expression is at least about a 2-fold decrease in expression relative to baseline expression. Expression can be reduced or increased by at least about 2 or more, or about 3, or about 4, or about 5, or about 6, or about 7, or about 8, or about 9, or about 10, or about 11, or about 12, or about 13, or about 14, or about 15 fold as compared to a comparative wild-type cell. One of skill in the art can monitor expression of the genes using methods such as RNA-sequencing, DNA microarrays, Real-time PCR, or Chromatin immunoprecipitation (ChIP) etc. Protein expression can be monitored using methods such as flow cytometry, Western blotting, 2-D gel electrophoresis or immunoassays etc.

In a further aspect, the T-cells are tissue-resident memory cells (TRM) or CD8+ T-cells. In one particular embodiment, the T-cells are autologous to the subject being treated. The methods of treating cancer, providing anti-tumor immunity, preventing relapse of cancer, and/or eliciting an anti-tumor response disclosed herein may further comprise, or consist essentially of, or yet further consist of administering to the subject an effective amount of a cytoreductive therapy. The cytoreductive therapy can be one or more of chemotherapy, immunotherapy, or radiation therapy.

Further provided herein is a method of treating cancer in a subject and/or eliciting an anti-tumor response comprising, or consisting essentially of, or yet further consisting of administering to the subject or contacting the tumor with an effective amount of the modified T-cells disclosed herein and/or the composition of this disclosure. The contacting can be performed in vitro, or alternatively in vivo, thereby providing immunotherapy to a subject such as for example, a human patient. A particular example of direct interaction is binding. A particular example of an indirect interaction is where one entity acts upon an intermediary molecule, which in turn acts upon the second referenced entity. Contacting as used herein includes in solution, in solid phase, in vitro, ex vivo, in a cell and in vivo. Contacting in vivo can be referred to as administering, or administration.

In one aspect, for the methods of treatments, the subject has, has had or is in need of treatment for cancer. In another aspect, the cancer is characterized as being hyporesponsive. In certain embodiments a subject has or is suspected of having a neoplastic disorder, neoplasia, tumor, malignancy or cancer. In some embodiments a subject in need of a treatment, cell or composition described herein has or is suspected of having a neoplastic disorder, neoplasia, tumor, malignancy or cancer.

The T-cells, population of T-cells, active agent and/or compositions provided herein may be administered either alone or in combination with diluents, known anti-cancer therapeutics, and/or with other components such as cytokines or other cell populations that are immunostimulatory. They may be administered as a first line therapy, a second line therapy, a third line therapy, or further therapy. Non-limiting examples of additional therapies include chemotherapeutics or biologics. Appropriate treatment regimens will be determined by the treating physician or veterinarian.

In one embodiment, the tumor is a solid tumor. The solid tumor could be a melanoma, a colon carcinoma, a breast carcinoma and/or a brain tumor. In one aspect, the cancer to be treated is a carcinoma, sarcoma, neuroblastoma, cervical cancer, hepatocellular cancer, mesothelioma, glioblastoma, myeloma, lymphoma, leukemia, adenoma, adenocarcinoma, glioma, glioblastoma, retinoblastoma, astrocytoma, oligodendrocytoma, meningioma, or melanoma.

The methods are useful to treat subjects such as humans, non-human primates (e.g., apes, gibbons, chimpanzees, orangutans, monkeys, macaques, and the like), domestic animals (e.g., dogs and cats), farm animals (e.g., horses, cows, goats, sheep, pigs) and experimental animals (e.g., mouse, rat, rabbit, guinea pig). A mammal can be any age or at any stage of development (e.g., an adult, teen, child, infant, or a mammal in utero). A mammal can be male or female. In certain embodiments the subject has or is suspected of having a neoplastic disorder, neoplasia, tumor, malignancy or cancer. In one aspect, the animal is treated as an animal model for a particular patient or tumor type, or can be used to assay combination therapies.

The methods disclosed herein may further comprise or alternatively consist essentially of, or yet further consists of administering to the subject an anti-tumor therapy other than the CAR therapy or T-cell therapy as disclosed herein. Accordingly, method aspects of the present disclosure relate to methods for inhibiting the growth of a tumor in a subject in need thereof and/or for treating a cancer patient in need thereof.

Further provided herein is a method of diagnosing a subject that may optionally be suspected of having cancer, comprising, or consisting essentially of, or yet further consisting of contacting a sample isolated from the subject with an agent that detects the presence of one or more genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 in the sample isolated from the subject, wherein the presence of the one or more genes at higher or lower than baseline expression levels is diagnostic of cancer. In one aspect, the method of diagnosing cancer in a subject comprises, or consists essentially of, or yet further consists of contacting tissue-resident memory cells (TRMs) of the cancer or a sample thereof with an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAG3, an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds CD28H (TMIGD2), and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of CD8^(+PD)1⁺, CD8⁺TIM3⁺, CD8⁺LAG3⁺, CD8⁺AMICA1⁺, CD8⁺CD28H⁺, CD8⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺, CD8⁺PD1⁺LAG3⁺, CD8⁺PD1⁺AMICA1⁺, CD8⁺PD1⁺CD28H⁺, CD8⁺PD1⁺CTLA4⁺, CD8⁺TIM3⁺LAG3⁺, CD8⁺TIM3⁺AMICA1⁺, CD8⁺TIM3⁺CD28H⁺, CD8⁺TIM3⁺CTLA4⁺, CD8⁺LAG3⁺CTLA4⁺, CD8⁺LAG3⁺AMICA1⁺, CD8⁺LAG3⁺CD28H⁺, CD8⁺PD1⁺TIM3⁺LAG3⁺, CD8⁺LAG3⁺PD1⁺AMICA1⁺, CD8⁺LAG3⁺PD1⁺CD28H⁺, CD8⁺PD1⁺LAG3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺AMICA1⁺′, CD8⁺PD1⁺TIM3⁺CTLA4⁺CD28H⁺′ or CD8⁺PD1⁺TIM3⁺CTLA4⁺AMICA⁺CD28H⁺′ TRMs, wherein a high frequency of one or more of these TRMs is diagnostic of cancer.

In another aspect, the method of diagnosing cancer in a subject comprises, or consists essentially of, or yet further consists of contacting tissue-resident memory cells (TRMs) isolated from the subject or cancer sample isolated from the subject, with an antibody or agent that recognizes and binds one or more proteins encoded by a gene set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 and, optionally, an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAG3, an antibody or agent that recognizes and binds CD28H (TMIGD2), an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds KLF3, an antibody or agent that recognizes and binds S1PR5, an antibody or agent that recognizes and binds S1PR1, an antibody or agent that recognizes and binds KLF2 and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of TRMs expressing these proteins, wherein a high frequency of TRMs expressing these proteins is diagnostic of cancer. The contacting can be performed in vitro, or alternatively in vivo. The subject can be any mammal, e.g., a human patient. A particular example of direct interaction is binding. A particular example of an indirect interaction is where one entity acts upon an intermediary molecule, which in turn acts upon the second referenced entity. Contacting as used herein includes in solution, in solid phase, in vitro, ex vivo, in a cell and in vivo. Contacting in vivo can be referred to as administering, or administration. Expression can be reduced or increased by at least about 2 or more, or about 3, or about 4, or about 5, or about 6, or about 7, or about 8, or about 9, or about 10, or about 11, or about 12, or about 13, or about 14, or about 15 fold as compared to a comparative wild-type cell. One of skill in the art can monitor expression of the genes using methods such as RNA-sequencing, DNA microarrays, Real-time PCR, or Chromatin immunoprecipitation (ChIP) etc. Protein expression can be monitored using methods such as flow cytometry, Western blotting, 2-D gel electrophoresis or immunoassays etc.

Additionally, disclosed herein is a method of determining the density of tissue-resident memory cells (TRMs) in a subject or sample isolated from the subject, e.g., a cancer, tumor, or sample thereof, the method comprising, or consisting essentially of, or yet further consisting of measuring expression of one or more gene selected from the group of 4-1BB, PD-1, CD103 or TIM3 or genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 in the sample, (e.g., cancer, tumor, or sample thereof), wherein higher or lower than baseline expression indicates higher density of TRMs in the sample (e.g., cancer, tumor, or sample thereof). Expression can be reduced or increased by at least about 2 or more, or about 3, or about 4, or about 5, or about 6, or about 7, or about 8, or about 9, or about 10, or about 11, or about 12, or about 13, or about 14, or about 15 fold as compared to a comparative wild-type cell. One of skill in the art can monitor expression of the genes using methods such as RNA-sequencing, DNA microarrays, Real-time PCR, or Chromatin immunoprecipitation (ChIP) etc. Protein expression can be monitored using methods such as flow cytometry, Western blotting, 2-D gel electrophoresis or immunoassays etc.

Further provided herein is a method of determining prognosis of a subject having cancer comprising, or consisting essentially of, or yet further consisting of measuring the density of tissue-resident memory cells (T_(RM)) in a sample isolated from the subject, (e.g., the cancer, tumor or a sample thereof), wherein a high density of T_(RM) indicates a more positive prognosis, e.g., an increased probability and/or duration of survival. In one aspect, the method of prognosis of a subject having cancer comprises, or consists essentially of, or yet further consists of contacting tissue-resident memory cells (TRMs) isolated from the subject with an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAG3, an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds CD28H (TMIGD2), and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of CD8⁺PD1⁺, CD8⁺TIM3⁺, CD8⁺LAG3⁺, CD8⁺AMICA1⁺, CD8⁺CD28H⁺, CD8⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺, CD8⁺PD1⁺LAG3⁺, CD8⁺PD1⁺AMICA1⁺, CD8⁺PD1⁺CD28H⁺, CD8⁺PD1⁺CTLA4⁺, CD8⁺TIM3⁺LAG3⁺, CD8⁺TIM3⁺AMICA1⁺, CD8⁺TIM3⁺CD28H⁺, CD8⁺TIM3⁺CTLA4⁺, CD8⁺LAG3⁺CTLA4⁺, CD8⁺LAG3⁺AMICA1⁺, CD8⁺LAG3⁻⁺CD28H⁺, CD8⁺PD1⁺TIM3⁺LAG3⁺, CD8⁺LAG3⁺PD1⁺AMICA1⁺, CD8⁺LAG3⁺PD1⁺CD28H⁺, CD8^(+PD)1⁺LAG3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺AMICA1⁺′, CD8⁺PD1^(.+)TIM3⁺CTLA4⁺CD28H⁺′ or CD8⁺PD1⁺TIM3⁺CTLA4⁺AMICA⁺CD28H⁺′ TRMs, wherein a high frequency of one or more of these TRMs indicates a more positive prognosis, e.g., an increased probability and/or duration of survival. In another aspect, the method of prognosis of a subject having cancer comprises, or consists essentially of, or yet further consists of contacting tissue-resident memory cells (TRMs) isolated from the subject, (e.g., of the cancer or a sample thereof) with an antibody or agent that recognizes and binds one or more proteins encoded by a gene set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 and, optionally, an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAG3, an antibody or agent that recognizes and binds CD28H (TMIGD2), an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds KLF3, an antibody or agent that recognizes and binds S1PR5, an antibody or agent that recognizes and binds S1PR1, an antibody or agent that recognizes and binds KLF2 and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of TRMs expressing these proteins, wherein a high frequency of TRMs expressing these proteins indicates a more positive prognosis, e.g., an increased probability and/or duration of survival.

In yet a further aspect, the method of determining prognosis of a subject having cancer comprises, or consists essentially of, or yet further consists of contacting tissue-resident memory cells (TRMs) isolated from the subject, e.g., of the cancer or a sample thereof; with an antibody or agent that recognizes and binds CD103 to determine the frequency of CD103+ TRMs or an antibody that recognizes and binds a protein encoded by a gene set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 to determine the frequency of TRMs expressing the protein, wherein a high or low frequency of TRMs expressing the protein indicates a more positive prognosis, e.g., an increased probability and/or duration of survival. The contacting can be performed in vitro, or alternatively in vivo. The subject can be a mammal, e.g., a human patient. A particular example of direct interaction is binding. A particular example of an indirect interaction is where one entity acts upon an intermediary molecule, which in turn acts upon the second referenced entity. Contacting as used herein includes in solution, in solid phase, in vitro, ex vivo, in a cell and in vivo. Contacting in vivo can be referred to as administering, or administration. In a separate aspect, the method of determining prognosis of a subject having cancer comprises, or consists essentially of, or yet further consists of measuring the density of CD103 or proteins encoded by one or more gene set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 in the sample, (e.g., a cancer or a sample thereof), wherein a high or low density of proteins indicates a more positive prognosis, and an increased probability and/or duration of survival.

For the above methods, an effective amount is administered, and administration of the cell or population serves to attenuate any symptom or prevent additional symptoms from arising. When administration is for the purposes of preventing, delaying or reducing the likelihood of cancer recurrence or metastasis or pathogen infection, the cell or compositions can be administered in advance of any visible or detectable symptom. Routes of administration include, but are not limited to, oral (such as a tablet, capsule or suspension), topical, transdermal, intranasal, vaginal, rectal, subcutaneous intravenous, intraarterial, intramuscular, intraosseous, intraperitoneal, epidural and intrathecal. In some embodiments, an effective amount may be delivered or administered into a cavity formed by the resection of tumor tissue (i.e. intracavity delivery) or directly into a tumor prior to resection (i.e. intratumoral delivery). In some embodiments, administration can be intravenously, intrathecally, intraperitoneally, intramuscularly, subcutaneously, or by other suitable means of administration.

Pharmaceutical compositions of the present disclosure may be administered in a manner appropriate to the disease to be treated or prevented. The quantity and frequency of administration will be determined by such factors as the condition of the patient, and the type and severity of the patient's disease, although appropriate dosages may be determined by clinical trials.

For the above methods, an effective amount is administered, and administration of the cell or population serves to attenuate any symptom or prevent additional symptoms from arising. When administration is for the purposes of preventing or reducing the likelihood of cancer recurrence or metastasis, the cell or compositions can be administered in advance of any visible or detectable symptom. Routes of administration include, but are not limited to, oral (such as a tablet, capsule or suspension), topical, transdermal, intranasal, vaginal, rectal, subcutaneous intravenous, intraarterial, intramuscular, intraosseous, intraperitoneal, epidural and intrathecal.

The methods provide one or more of: (1) preventing the symptoms or disease from occurring in a subject that is predisposed or does not yet display symptoms of the disease; (2) inhibiting the disease or arresting its development; or (3) ameliorating or causing regression or relapse of the disease or the symptoms of the disease. As understood in the art, “treatment” is an approach for obtaining beneficial or desired results, including clinical results. For the purposes of the present technology, beneficial or desired results can include one or more, but are not limited to, alleviation or amelioration of one or more symptoms, diminishment of extent of a condition (including a disease), stabilized (i.e., not worsening) state of a condition (including disease), delay or slowing of condition (including disease), progression, amelioration or palliation of the condition (including disease), states and remission (whether partial or total), whether detectable or undetectable. Treatments containing the disclosed compositions and methods can be first line, second line, third line, fourth line, fifth line therapy and are intended to be used as a sole therapy or in combination with other appropriate therapies e.g., surgical recession, chemotherapy, radiation. In one aspect, treatment excludes prophylaxis.

Also described herein is a method of determining the responsiveness of a subject having cancer to immunotherapy comprising, or consisting essentially of, or yet further consisting of contacting tissue-resident memory cells (TRMs) isolated from the subject, e.g., of the cancer or a sample thereof, with an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAG3, an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds CD28H (TMIGD2), and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of CD8⁺PD1⁺, CD8⁺TIM3⁺, CD8⁺LAG3⁺, CD8⁺AMICA1⁺, CD8⁺CD28H⁺, CD8⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺, CD8⁺PD1⁺LAG3⁺, CD8⁺PD1⁺AMICA1⁺, CD8⁺PD1⁺CD28H⁺, CD8⁺PD1⁺CTLA4⁺, CD8⁺TIM3⁺LAG3⁺, CD8⁺TIM3⁺AMICA1⁺, CD8⁺TIM3⁺CD28H⁺, CD8⁺TIM3⁺CTLA4⁺, CD8⁺LAG3⁺CTLA4⁺, CD8⁺LAG3⁺AMICA1⁺, CD8⁺LAG3⁺CD28H⁺, CD8^(+PD)1⁺TIM3⁺LAG3⁺, CD8⁺LAG3⁺PD1⁺AMICA1⁺, CD8⁺LAG3⁺PD1⁺CD28H⁺, CD8⁺PD1⁺LAG3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺AMICA1⁺′, CD8⁺PD1⁺TIM3⁺CTLA4⁺CD28H⁺′ or CD8⁺PD1⁺TIM3⁻⁺CTLA4⁺AMICA⁺CD28H⁺′TRMs, wherein a high frequency of one or more of these TRMs indicates responsiveness to immunotherapy. In one aspect, the method of determining the responsiveness of a subject having cancer to immunotherapy comprises, or consists essentially of, or yet further consists of contacting tissue-resident memory cells (TRMs) isolated from the subject, e.g., of the cancer or a sample thereof, with an antibody or agent that recognizes and binds one or more proteins encoded by a gene set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 and, optionally, an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAG3, an antibody or agent that recognizes and binds CD28H (TMIGD2), an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds KLF3, an antibody or agent that recognizes and binds S1PR5, an

antibody or agent that recognizes and binds S1PR1, an antibody or agent that recognizes and binds KLF2 and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of TRMs expressing these proteins, wherein a high frequency of TRMs expressing these proteins indicates responsiveness to immunotherapy. For any of the methods disclosed herein, the TRMs may comprise, or consist essentially of, or yet further consist of CD19−CD20−CD14−CD56−CD4−CD45+CD3+CD8+ T-cells.

Further disclosed are methods of identifying a subject that will or is likely to respond to a cancer therapy, comprising, or consisting essentially of, or yet further consisting of contacting a sample isolated from the subject with an agent that detects the presence of one or more genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 in the sample, (e.g., cancer or a sample thereof), wherein the presence of the one or more genes at higher or lower than baseline expression levels indicates that the subject is likely to respond to cancer therapy. In one aspect, the baseline expression is normalized mean gene expression. In another aspect, the higher than baseline expression is at least about a 2-fold increase in expression relative to baseline expression and/or lower than baseline expression is at least about a 2-fold decrease in expression relative to baseline expression. Expression can be reduced or increased by at least about 2 or more, or about 3, or about 4, or about 5, or about 6, or about 7, or about 8, or about 9, or about 10, or about 11, or about 12, or about 13, or about 14, or about 15 fold as compared to a comparative wild-type cell. One of skill in the art can monitor expression of the genes using methods such as RNA-sequencing, DNA microarrays, Real-time PCR, or Chromatin immunoprecipitation (ChIP) etc. Protein expression can be monitored using methods such as flow cytometry, Western blotting, 2-D gel electrophoresis or immunoassays etc. The method may further comprise, or consist essentially of, or yet further consist of administering a cancer therapy to the subject. The cancer therapy or cytoreductive therapy can be chemotherapy, immunotherapy, radiation therapy, and/or administering to the subject or contacting the tumor with an effective amount of the modified T-cells and/or the composition of this disclosure.

The cancer, tumor, or sample can be contacted with an agent, optionally including a detectable label or tag. In one aspect, the detectable label or tag can comprise, or consist essentially of, or yet further consist of a radioisotope, a metal, horseradish peroxidase, alkaline phosphatase, avidin or biotin. In another aspect, the agent can comprise, or consist essentially of, or yet further consist of a polypeptide that binds to an expression product encoded by the gene, or a polynucleotide that hybridizes to a nucleic acid sequence encoding all or a portion of the gene. The polypeptide may comprise, or consist essentially of, or yet further consist of an antibody, an antigen binding fragment thereof, or a receptor that binds to the gene. In one aspect, the antibody is an IgG, IgA, IgM, IgE or IgD, or a subclass thereof. In another aspect, the IgG antibody is an IgG1, IgG2, IgG3 or IgG4. The antigen binding fragment can be a Fab, Fab′, F(ab′)2, Fv, Fd, single-chain Fvs (scFv), disulfide-linked Fvs (sdFv) or VL or VH. In one aspect, the agent is contacted with the cancer, tumor, or sample in conditions under which it can bind to the gene it targets. The contacting can be performed in vitro, or alternatively in vivo. A particular example of direct interaction is binding. A particular example of an indirect interaction is where one entity acts upon an intermediary molecule, which in turn acts upon the second referenced entity. Contacting as used herein includes in solution, in solid phase, in vitro, ex vivo, in a cell and in vivo. Contacting in vivo can be referred to as administering, or administration.

The methods of this disclosure the method comprise, or consist essentially of, or yet further consist of detection by immunohistochemistry (IHC), in-situ hybridization (ISH), ELISA, immunoprecipitation, immunofluorescence, chemiluminescence, radioactivity, X-ray, nucleic acid hybridization, protein-protein interaction, immunoprecipitation, flow cytometry, Western blotting, polymerase chain reaction, DNA transcription, Northern blotting and/or Southern blotting. The sample may comprise, or consist essentially of, or yet further consist of cells, tissue, an organ biopsy, an epithelial tissue, a lung, respiratory or airway tissue or organ, a circulatory tissue or organ, a skin tissue, bone tissue, muscle tissue, head, neck, brain, skin, bone and/or blood sample. In another aspect, the sample comprises one or more of sputum, serum, plasma, lymph, cystic fluid, urine, stool, cerebrospinal fluid, ascite fluid, blood, or a tissue. While the cancer or tumor described herein can be an epithelial, a head, neck, lung, lung, prostate, colon, pancreas, esophagus, liver, skin, kidney, adrenal gland, brain, or comprises a lymphoma, breast, endometrium, uterus, ovary, testes, lung, prostate, colon, pancreas, esophagus, liver, skin, kidney, adrenal gland and/or brain cancer or tumor, a metastasis or recurring tumor, cancer or neoplasia, a non-small cell lung cancer (NSCLC) and/or head and neck squamous cell cancer (HNSCC). In a further aspect, the methods of this disclosure may comprise, or consist essentially of, or yet further consist of detecting in the subject, the cells or the sample the number or density of Trm cells that are CD19−CD20−CD14−CD56−CD4−CD45+CD3+CD8+ T-cells.

Kits

Finally, provided herein is a kit comprising, or consisting essentially of, or yet further consisting of one or more of the modified T-cells and/or the composition of this disclosure and instructions for use. In one particular aspect, the present disclosure provides kits for performing the methods of this disclosure as well as instructions for carrying out the methods of the present disclosure.

The kits are useful for detecting the presence of cancer such as B-cell lymphoma in a biological sample e.g., any bodily fluid including, but not limited to, e.g., sputum, serum, plasma, lymph, cystic fluid, urine, stool, cerebrospinal fluid, acitic fluid or blood and including biopsy samples of body tissue. The test samples may also be a tumor cell, a normal cell adjacent to a tumor, a normal cell corresponding to the tumor tissue type, a blood cell, a peripheral blood lymphocyte, or combinations thereof. The test sample used in the above-described method will vary based on the assay format, nature of the detection method and the tissues, cells or extracts used as the sample to be assayed. Methods for preparing protein extracts or membrane extracts of cells are known in the art and can be readily adapted in order to obtain a sample which is compatible with the system utilized.

The kit components, (e.g., reagents) can be packaged in a suitable container. The kit can also comprise, or alternatively consist essentially of, or yet further consist of, e.g., a buffering agent, a preservative or a protein-stabilizing agent. The kit can further comprise, or alternatively consist essentially of, or yet further consist of components necessary for detecting the detectable-label, e.g., an enzyme or a substrate. The kit can also contain a control sample or a series of control samples, which can be assayed and compared to the test sample. Each component of the kit can be enclosed within an individual container and all of the various containers can be within a single package, along with instructions for interpreting the results of the assays performed using the kit. The kits of the present disclosure may contain a written product on or in the kit container. The written product describes how to use the reagents contained in the kit.

As amenable, these suggested kit components may be packaged in a manner customary for use by those of skill in the art. For example, these suggested kit components may be provided in solution or as a liquid dispersion or the like.

Modes for Carrying Out the Disclosure

Using single-cell and bulk transcriptomic analysis of purified populations of TRM and non-T_(RM) cells present in tumor and normal lung tissue from patients with lung cancer, Applicants identified a distinct population of highly functional TRM cells present exclusively in the tumors. These TRM cells proliferate, display clonal expansion and express high levels of TIM3, CXCL13 and CD39. They also expressed high levels of PD-1 but show no features of exhaustion. Rather, these ‘highly functional’ TRM cells are the key cell types contributing to the robust anti-tumor responses induced by PD-1 inhibitors in some cancer patients. Because PD-1 expression was also observed in TRM cells in the normal lung, without being bound by theory, Applicant believes that PD1 inhibitors may have the potential to non-specifically reactivate quiescent TRM cells present in normal lung and presumably other tissues and cause the clinically recognised immune-related toxicities. These findings have implications for the design of therapies that preferentially activate “highly functional” TRM cells in tumors while minimizing toxicity.

In lung cancer and many other solid tumors, the presence of an adaptive anti-tumor immune response is positively correlated with patient survival.′ This response is mediated primarily by CD8⁺cytotoxic T lymphocytes (CTLs). Because CTLs in tumors are chronically activated, they can become “exhausted,” a hyporesponsive state, that prevents inflammatory damage to healthy tissue in the setting of infection.² Exhaustion involves up-regulation of surface inhibitory molecules, such as PD-1 and TIM3.³ PD-1 inhibitors have revolutionized cancer treatment by inducing durable responses in some patients.⁴ Given the association of PD-1 with exhaustion and the description of CTLs expressing PD-1 in human cancers, exhausted CTLs are generally assumed to be the cells reactivated by anti-PD-1 therapy, though definitive evidence for this is lacking in humans.⁵

Though PD-1 inhibitors can eradicate tumors in some cancer patients, they also lead to serious adverse immune-mediated reactions,⁶ calling for research to identify features unique to tumor-reactive CTLs. One subset of CTLs that may harbor such distinctive properties are tissue-resident memory T cells (T_(RM)), which mediate the response to anti-tumor vaccines' and facilitate rejection of tumors in animal models.⁸ TRM responses have also recently been shown by Applicant⁹ and others¹⁰ to associate with better survival in human solid tumors. The molecular features of TRM cells' response has been characterized in the setting of infection and involves rapid clonal expansion and upregulation of molecules aiding recruitment and activation of additional immune cells, alongside the traditional effector functions of CTL.¹¹ However, the molecular features that drive the anti-tumor functions of human TRM cells was previously unknown. To address this question, the Applicants compared the transcriptome of TRM and non-T_(RM) CTLs present in tumor and normal lung tissue samples.

CD103 Expressing CTLs in Human Lungs are Enriched for Core Tissue Residency Features

CTLs were isolated from lung tumor and adjacent uninvolved lung tissue samples provided by patients (n=30) with treatment-naïve early-stage non-small cell lung cancer (NSCLC), then sorted according to CD103 expression to separate TRM from non-T_(RM) cells (FIG. 7). The transcriptomes of each population were determined by RNA sequencing (RNA-Seq). Unbiased visualization of RNA-seq data of CTLs from normal lung using 2D t-stochastic neighbor embedding (tSNE) revealed the distinct nature of CD103⁺and CD103⁻ CTLs (FIG. 1A); nearly 700 transcripts were differentially expressed between the two populations (FIG. 1B and Table 3). Transcripts expressed at higher levels in CD103⁺ CTLs included several previously linked to TRM phenotype, such as S1PR1, S1PR5, ITGA1, RBPJ^(12,13). Gene set enrichment analysis (GSEA) of lung CD103⁺ CTLs showed that the pattern of these transcripts' expression correlated with a core tissue residency signature¹⁴, previously defined by integration of transcriptomic datasets generated from murine CD8⁺ TRM cells isolated from several organs (FIG. 1C). The Applicants confirmed that lung CD103⁺ CTLs express CD49A¹³, an established T_(RM) molecule, and do not express KLRG1, linked to effector cells¹³, at the protein level (FIG. 1D and FIG. 8). Together, these data confirm that CD103⁺ CTLs in human lungs are highly enriched for T_(RM) cells; for simplicity, hereafter CD103⁺ CTLs are referred to as TRM cells and CD103⁻ CTLs as non-T_(RM) cells.

T_(RM) Cells in Human Lungs are Transcriptionally Distinct from Previously Characterized TRM Cells

Differentially expressed transcripts between lung CD103⁺and CD103⁻ CTLs were compared with those reported for other TRM cells. The comparison with human skin TRM cells¹⁵ revealed limited overlap; the majority of transcripts differentially expressed in skin TRM cells relative to other CTLs were not differentially expressed between lung TRM and non-TRM cells (FIG. 1E). Similarly, comparisons with gene signatures of murine TRM cells isolated from multiple organs¹⁴ revealed limited overlap (FIG. 1E, FIG. 1F), although core tissue-residency features were well preserved. However, those differentially expressed transcripts that were not preserved across organs, or species, were not significantly enriched (FIG. 55). Thus, the transcriptional program, outside of a core tissue residency program of human lung TRM cells is quite distinct from that of human skin TRM cells and murine TRM cells present in several organs, and importantly, many of the features observed in human lung TRM cells have not been previously reported (Table 3)¹³.

T_(RM) Cells in Normal Lung and Lung Tumors Share Tissue Residency Features, but are Otherwise Distinct

The Applicants analyzed whether TRM cells in lung tumors share tissue residency features with TRM cells in adjacent normal lung tissue. Gene set enrichment analysis (GSEA) of lung tumor-infiltrating CD103⁺ CTLs showed that their transcript expression correlated with the core murine tissue residency signature¹⁴, implying that even in tumors, CD103 expression defines TRM cells (FIG. 2A). Furthermore, over 300 transcripts were differentially expressed between CD103⁺and CD103⁻ CTLs present in lung tumors, and these included several transcripts previously linked to TRM cells (Table 4). However, CD103⁺and CD103⁻ CTLs from normal lung and tumor clustered separately (as 4 subpopulations) on tSNE plots (FIG. 2B). Nearly two-thirds of the T_(RM) properties, i.e., transcripts differentially expressed between CD103⁺and CD103⁻ CTLs, in tumors were different from those of normal lung TRM cells (FIG. 2C and Table 4).

Standard and weighted co-expression analysis (Methods) of the 89 ‘shared tissue residency’ transcripts (FIG. 2D) revealed a number of novel genes whose expression was highly correlated with known tissue residency (T_(RM)) genes, showing that their products play important roles in the development, trafficking or function of lung tumor-infiltrating TRM cells (FIG. 2E, FIG. 2F). Notable examples encoding products functioning in tumor TRM migration or retention include GPR25, SRGAP3, AMICA1, CAPG, ADAM19, and NUAK2 (FIG. 2E-2F).

Another ‘shared tissue residency’ transcript was PDCD1, encoding PD-1 (FIG. 2E-2F). The Applicants confirmed at the protein level that PD-1 is expressed at higher levels in both tumor and lung TRM cells compared to non-T_(RM) cells (FIG. 2G and FIG. 9). Although PD-1 expression is considered typical of exhausted T cells³, recent reports have suggested that high PD-1 expression is a tissue residency feature of brain TRM cells independent of antigen stimulation^(16,17), and of murine TRM cells from multiple organ systems¹⁴. In support of the conclusion that high expression of PD-1 reflects tissue residency rather than exhaustion, ex vivo stimulation of TRM and non-T_(RM) cells isolated from both lung and tumor tissue resulted in robust up-regulation of TCR-activation-induced genes (NR4A1, CD69, TNFRSF9 (4-1BB), EGR2) and cytokines (TNF, IFNG) (FIG. 2H). In addition to PDCD1, ‘shared tissue-residency’ transcripts included several (SPRY1¹⁸, CD226¹⁹, TMIGD2²⁰, CLNK²¹, KLRC1²²) that encode products reported to play a regulatory role in other immune cell types (FIG. 2F lower panel). The expression of these inhibitory molecules restrains the functional activity of tumor TRM cells.

Tumor TRM Cells Proliferate, Express the Inhibitory Checkpoint TIM3 and Markers of Enhanced Function

To identify features unique to tumor TRM cells, the Applicants compared their transcriptome to those of lung TRM cells and non-T_(RM) cells in both normal lung and tumors and detected 93 differentially expressed transcripts (FIG. 3A and Table 5). Reactome pathway analysis of ‘tumor T_(RM)-enriched’ transcripts showed significant enrichment for transcripts encoding components of the canonical cell cycle, mitosis and DNA replication machinery (FIG. 3B). The tumor TRM subset thus appears to be highly enriched for proliferating CTLs, presumably responding to tumor-associated antigens (TAA). Unique molecular identifier (UMI)-based T cell receptor (TCR) sequencing assays revealed a more restricted TCR repertoire in TRM cells compared to non-T_(RM) cells in tumors, as shown by significantly lower Shannon-Wiener and Inverse Simpson diversity indices (FIG. 3C and Table 6). Furthermore, the tumor TRM population contained a higher percentage of expanded clonotypes (73% vs. 52% in tumor TRM vs. non-T_(RM) populations) (FIG. 3D). The top expanded clonotype in each patient comprised, on average, 19% of all the clonotypes detected in TRM cells (FIG. 3D and Table 6), showing marked expansion of a single TAA-specific T cell clone in the tumor TRM population. In most patients, some expanded TCR clonotypes detected in the tumor TRM population were shared with cells in the non-T_(RM) population present in same tumor samples (Table 6), reflecting either derivation from common precursors or conversion of tumor TRM cells to effector non-T_(RM) cells.

‘Tumor T_(RM)-enriched’ transcripts that were highly correlated with cell cycle genes encode products with important functions and reflect the molecular features of TRM cells that are actively expanding in response to TAA. HAVCR2, encoding the co-inhibitory checkpoint molecule TIM3, was most correlated and connected with cell cycle genes (FIG. 3E-3F). TIM3 expression is a unique feature of lung tumor TRM cells that is not necessarily linked to exhaustion, as the other transcripts that correlated with expression of TIM3 and cell cycle genes encode molecules that could confer superior functionality such as CD39 (encoded by ENTPD1)²³, LAYN²⁴, CXCL13²⁵, CCL3²⁶, TNFSF4²⁷ (OX-40 ligand), as well as a marker of antigen-specific engagement (4-1BB, encoded by TNFRSF9) (FIG. 3E-3F)²⁸. Robust expression of this set of molecules was observed in neither human lung TRM cells nor in the mouse TRM signature, indicating that the tumor TRM population contains novel cell subsets.

Single-Cell Transcriptomic Analysis Reveals Previously Uncharacterized TRM Subsets

To determine whether ‘tumor T_(RM)-enriched’ transcripts are expressed in all or only a subset of the tumor TRM population, the Applicants performed single-cell RNA-Seq assays in CD103⁺ and CD103⁻ CTLs isolated from tumor and adjacent normal lung tissue from 12 patients with early-stage lung cancer. Analysis of the ˜12,000 single-cell transcriptomes revealed 5 clusters of TRM cells and 4 clusters of non-T_(RM) cells (FIG. 4A, FIG. 4B). Among the 5 TRM clusters, a greater proportion of cells in the tumor TRM population compared with the lung TRM population was observed in clusters 1-3, while clusters 4 and 5 contained more lung TRM cells (FIG. 4B-4C). Most strikingly, clusters 1-3 contained very few lung TRM cells (FIG. 4C). The ‘tumor T_(RM)-enriched’ transcripts detected in Applicants' analysis of bulk populations (FIG. 3A) were contributed by cells in these subsets.

In agreement with that conclusion, cells in cluster 1 expressed high levels of the 25 cell cycle-related ‘tumor T_(RM)-enriched’ transcripts (FIG. 4D)²⁹, indicating that the enrichment of cell cycle transcripts in the bulk tumor T_(RM) population was contributed by this relatively small subset. Because these cells are actively proliferating, they represent TAA-specific cells. The majority of cells in this cycling cluster were from the tumor TRM population (FIG. 4E). These cells, along with those in the larger cluster 2, were highly enriched for other prominent ‘tumor T_(RM)-enriched’ transcripts like HAVCR2 (TIM3), including those encoding products that could confer superior functionality (e.g., CD39, LAYN, CXCL13, CCL3; FIG. 4F). This shared expression pattern shows that the cycling cluster simply represent cells in cluster 2 that are entering the cell cycle. Confirming this idea, cell-state hierarchy maps of all tumor TRM cells, constructed using Monocle2³⁰, revealed that cells in cluster 2 were most similar to the cycling TRM cells (cluster 1) (FIG. 4G and FIG. 10). Additionally, the Applicants found that when performing hierarchical clustering of these cells, the proliferating cluster 1 clustered more with cells assigned to cluster 2 than the other TRM clusters (FIG. 4F). This finding was corroborated when Applicants calculated the average distance in principle component space between each cell in cluster 1 to the other TRM clusters (FIG. 10D). Overall, the single-cell transcriptome analysis uncovered additional distinct subsets of tumor TRM cells that have not previously been described and play an important role in anti-tumor immune responses.

A Subset of Tumor TRM Cells has a Transcriptional Program Indicative of Superior Functional Properties

To dissect the molecular properties unique to tumor-infiltrating TRM cells in each of the 4 larger clusters, the Applicants performed multiple pair-wise single-cell differential gene expression analyses (Methods). Over 250 differentially expressed genes showed higher expression in any one of the Applicants' clusters (FIG. 5A and Table 7), indicating that cells in different clusters had divergent gene expression programs. For example, cells in cluster 3 were highly enriched for transcripts encoding heat shock proteins (e.g., HSPA1A, HSPA1B and HSP90AA1), whereas cells in cluster 5, comprising TRM cells from normal lung and tumor tissue, expressed high levels of IL7R, which encodes the IL-7 receptor, a marker of memory precursor cells³¹, and transcripts such as GPR183³², MYADM³³, VIM³⁴ and ANKRD28³⁵, which encode proteins involved in cell migration and tissue homing (FIG. 5A, FIG. 5B).

Because of their close relationship with cycling TRM cells (FIG. 4D, FIG. 4G), the Applicants' analysis focused on TRM cells in cluster 2. The 91 transcripts expressed more highly by these cells than other TRM clusters (FIG. 5A) included several with encoded products linked to cytotoxic activity such as PRF1, GZMB, GZMA, CTSW³¹, RAB27A³⁶, ITGAE³⁷ and CRTAM³¹ (FIG. 5C and FIG. 11), as well as a number encoding effector cytokines and chemokines, such as IFN-γ, CCL3, CXCL13, IL17A and IL26. Cluster 2 also expressed high levels of transcripts encoding transcription factors known to promote the survival of memory or effector CTLs (ID2³⁸, STAT3³⁹, ZEB2⁴° and ETS-1⁴¹) or that are involved in establishing and maintaining tissue residency (RBPJ, a key player in Notch signaling¹³, and BLIMP1⁴², encoded by PRDM1) (FIG. 5C and FIG. 11). TRM cells in cluster 2 also highly expressed ENTPD1 (FIG. 5B, FIG. 5C), which encodes CD39, an ectonucleotidase that cleaves ATP, which may protect this TRM subset from ATP-induced cell death in the ATP-rich tumor microenvironment²³. This expression pattern confers highly effective and sustained anti-tumor immune function; in combination with earlier results, it was determined that this ‘highly functional’ TRM subset represents TAA-specific cells that proliferate in tumors.

T_(RM) cells in cluster 2 expressed the highest levels of PDCD1 transcripts (FIG. 5A) and were enriched for transcripts encoding other molecules linked to exhaustion such as TIM3, TIGIT¹⁹, and CTLA4³, and inhibitors of TCR-induced signaling and activation like CBLB, SLAP, DUSP4, PTPN22 and NR3C1 (glucocorticoid receptor) (FIG. 5A-5C) and FIG. 11)⁴³⁻⁴⁶. Nonetheless, these TRM cells exhibited a transcriptional program suggestive of superior effector properties and cell proliferation, and expressed high transcript levels for several co-stimulatory molecules such as 4-1BB, ICOS and GITR (TNFRSF18) (FIG. 5C and FIG. 11)³. More specifically, PDCD1-expressing TRM cells in cluster 2 expressed relatively higher levels of IFNG, CCL3, and CXCL13 transcripts compared with cells not expressing PDCD1 in that cluster and other tumor-infiltrating TRM and non-T_(RM) cells (FIG. 5D). This co-expression program appeared to be specific to the tumor TRM compartment, given it was also reflected in a SAVER-imputed co-expression profiled being identified specifically in the TRM subsets, but not the non-TRM subsets (FIG. 11B). Overall, these findings agree with the bulk RNA-Seq analysis, indicating that inside this specific subset of CTLs expression of inhibitory molecules, like PD-1, does not reflect exhaustion. Instead, it prevents TCR-activation-induced cell death to sustain robust anti-tumor CTL responses^(23,47).

PD-1- and TIM3-Expressing Tumor-Infiltrating TRM Cells are not Exhausted

To further address whether PDCD1-expressing TRM cells in cluster 2 (highly functional ‘TRM cells’) were exhausted or functionally active, the Applicants performed single-cell RNA-seq in tumor-infiltrating TRM and non-T_(RM) cells, using SMART-seq2 for paired transcriptomic and TCR clonotype analysis³¹. The TCRβ chains (Methods) in 81% of single cells, the TCRα chain in 77%, and both chains in 70% of cells were reconstructed. As expected, clonally expanded tumor-infiltrating TRM cells, which are reactive to TAA, were significantly enriched for genes specific to ‘highly functional’ TRM cells (FIG. 6A). Among tumor-infiltrating CTLs, a greater proportion of TIM3-expressing (Methods) TRM cells were clonally expanded compared with other TRM and non-T_(RM) cells (FIG. 6B). As expected, TIM3-expressing TRM cells were significantly enriched for key effector cytokines and cytotoxicity transcripts, despite expressing higher levels of PDCD1 (FIG. 6C and FIG. 12). Importantly, it was discovered that a greater proportion of IFNG-expressing cells co-expressed PDCD1 among TIM3-expressing TRM cells compared with non-T_(RM) cells (FIG. 6D).

The higher sensitivity of the SMART-seq2 assay compared to the high-throughput 10× genomics platform also allowed better co-expression analysis due to lower dropout rates³¹. Co-expression analysis showed that expression of PDCD1 and HAVCR2 (TIM3) correlated with that of activation markers (TNFRSF9 and CD74), IFNG and cytotoxicity-related transcripts more strongly in TRM cells compared with non-T_(RM) cells (FIG. 6E). Specifically, IFNG and PDCD1 expression levels were better correlated in TIM3-expressing TRM cells compared with non-T_(RM) cells (FIG. 6D-FIG. 6E), and the proportion of cells strongly co-expressing these transcripts was notably higher (30% vs. 1%). Overall, these results strongly support that PD1 and TIM3 expressing tumor-infiltrating TRM are not exhausted, but instead are highly functional and are enriched for transcripts (IFNG, PRF1, GZMA) encoding for molecules linked to effector functions.

In keeping with the transcriptomic assays performed by Applicants, it was found that tumor-infiltrating TRM cells that co-expressed PD-1, when stimulated ex-vivo, had significantly higher percentage of cells expressing effector cytokines when compared to the non-TRM CTLs that co-expressed PD-1 (FIG. 50, FIG. 56A). Analysis directly ex-vivo demonstrated there was also greater expression of cytotoxic-associated proteins, granzyme A and granzyme B, in the PD-1+ TRM cells when compared to the PD-1+non-TRM CTLs in the tumor (FIG. 50, FIG. 56B). These data verify that PD-1 expression in the TRM subset of tumor-infiltrating CTLs does not reflect dysfunctional properties.

The Applicants evaluated the protein expression of selected molecules to better discern the tumor-infiltrating TRM subsets. Multi-parameter protein analysis of CTLs present in tumors and adjacent normal lung revealed a subset of TRM (CD103⁺) cells localized distinctly when the data was visualized in 2D space (FIG. 6F, left). This subset consisted of cells only from tumor tissue (circle, FIG. 6F), and uniquely expressed high levels of TIM3 and lacked IL-7R, indicating that this cluster is the same as the ‘highly functional’ TIM3-expressing TRM cluster (cluster 2) identified by single-cell RNA analysis (FIG. 6F, FIG. 6G and FIG. 13A). Consistent with the single-cell transcriptome analysis, the TIM3-expressing TRM cluster expressed higher levels of CD39, PD-1 and 4-1BB (FIG. 6F, FIG. 6H and FIG. 13B). PD-1 and TIM3 expression levels were also positively correlated with expression of 4-1BB, which is expressed following TCR engagement by antigen (FIG. 6I), indicating that these cells are highly enriched for TAA-specific cells. TIM3-expressing CTLs were detected among tumor-infiltrating TRM cells isolated from both lung cancer and head and neck squamous cell carcinoma (HNSCC) samples (FIG. 6G, right and FIG. 13B, FIG. 13C), but not among non-T_(RM) cells in these treatment naïve tumors or TRM cells in lung. Multi-color immunohistochemistry was used to confirm the presence of TIM-3-expressing TRM cells in lung tumor samples, which also showed enrichment of this subset in TIL^(hi)T_(RM) ^(hi) “immune hot” tumors (FIG. 53 and Table 7). These findings confirm, at the protein level, the specificity of this ‘highly functional’ T_(RM) subset to tumors.

Given the highly specific expression of TIM3 in the subset of ‘highly functional’ tumor-infiltrating TRM cells, the TIM3 expression levels in the Applicants previous bulk CD8⁺ TIL transcriptome data⁹ was used as a surrogate to assess the relative magnitude of this ‘highly functional’ TRM subset in tumors, and thus relate this variable to features linked to better survival outcomes such as TRM density in tumors. The Applicants found a strong positive correlation between transcript levels of TIM3 and CD103 (ITGAE) in tumor-infiltrating CTLs (FIG. 6K), showing that tumors with high TRM density (high ITGAE levels) harbor more ‘highly functional’ TIM3-expressing TRM cells.

Discussion

The disclosed bulk and single-cell transcriptomic analysis of lung and tumor-infiltrating TRM cells reveal that human TRM cells include at least 4 distinct subsets. Although human tumor-infiltrating TRM cells shared some core tissue residency features with those previously described from mouse models of infection and tumors, the vast majority of their molecular features were quite distinct. The most striking discovery was the identification of a ‘highly functional’ TIM3-expressing TRM subset present exclusively in tumors. This subset, although expressing high levels of PD-1 and other molecules previously thought to reflect exhaustion, exhibited a transcriptional program indicative of superior effector, survival and tissue residency properties and proliferated in the tumor milieu.

The Applicants defined a core set of genes commonly expressed in both lung and tumor TRM cells, including a number of novel genes whose expression was highly correlated with known tissue residency (TRM) genes. Any one of these genes may also be important for the development, trafficking or function of lung or lung tumor-infiltrating TRM cells. Some notable examples known or likely to have such functions are GPR25, whose closest homolog, GPR15⁴⁸, enables homing of T cell subsets to and retention in the colon; AMICA⁴⁹, encoding JAML (junctional adhesion molecule-like), which contributes to the proliferation and cytokine release of skin-resident γδT cells; and SRGAP, whose product functions in neuronal migration⁵⁰.

PDCD1 was a prominent hit in the ‘shared lung tissue residency’ gene list, and its expression was confirmed at the protein level in both lung and tumor TRM cells. The fact that PD-1 was expressed in TRM cells isolated from normal lung tissue of subjects with no active infection shows that PD-1 is constitutively expressed by human lung TRM cells, as has been recently described for brain TRM cells¹⁶. As PD-1 is expressed most highly by ‘highly functional’ TIM3-expressing tumor-infiltrating TRM cells, they may be the major cellular targets of anti-PD-1 therapy. Differences in the magnitude of this population of TRMs could thus be an explanation for the variation in the clinical response to PD-1 inhibitors, and non-responders may have defects in the de-novo generation of highly functional TIM3-expressing TRM cells. The constitutive expression of PD-1 by TRM cells in the normal lung and presumably other organs (skin, gut and pituitary gland) raises the possibility that anti-PD-1 therapy may non-specifically activate potentially self-reactive TRM cells to cause adverse immune reactions such as pneumonitis, dermatitis, colitis and hypophysitis⁶.

These findings raise the question of which molecular players are essential for the generation and maintenance of this novel ‘highly functional’ TIM3-expressing subset of TRM cells. This analysis identified a number of potential transcription factors (e.g., STAT3, ID2, ZEB2, ETS-1) and other molecules (e.g., PTPN22, DUSP4, LAYN, KRT86, CD39) that are uniquely expressed in this subset and could thus be key players in their development.

The results herein also provide a rationale for assessing tumor TRM subsets in both early and late phase studies of novel immunotherapies and cancer vaccines to provide early proof for efficacy as well as potential response biomarkers. The ‘highly functional’ TIM3-expressing TRM subset can be readily isolated from tumor samples using the surface markers identified herein and expanded in vitro to screen and test Tom-targeted adoptive T cell therapies. The highly functional TIM3-expressing TRM subset can be enriched for TAA-specific cells, and specifically expanding this TRM subset will improve the efficacy of adoptive T cell therapies.

It is to be understood that the present disclosure is not limited to particular aspects described, as such may, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting, since the scope of the present disclosure will be limited only by the appended claims.

A number of embodiments of the disclosure have been described. Nevertheless, it will be understood that various modifications may be made without departing from the spirit and scope of the disclosure. Accordingly, the following examples are intended to illustrate but not limit the scope of disclosure described in the claims.

It is to be inferred without explicit recitation and unless otherwise intended, that when the present technology relates to a polypeptide, protein, polynucleotide or antibody, an equivalent or a biologically equivalent of such is intended within the scope of the present technology.

Throughout this disclosure, various publications, patents and published patent specifications are referenced by an identifying citation. All publications, patent applications, patents, and other references mentioned herein are expressly incorporated by reference in their entirety, to the same extent as if each were incorporated by reference individually. In case of conflict, the present specification, including definitions, will control.

The entirety of each patent, patent application, publication or any other reference or document cited herein hereby is incorporated by reference. In case of conflict, the specification, including definitions, will control.

Citation of any patent, patent application, publication or any other document is not an admission that any of the foregoing is pertinent prior art, nor does it constitute any admission as to the contents or date of these publications or documents.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present disclosure, suitable methods and materials are described herein.

All of the features disclosed herein may be combined in any combination. Each feature disclosed in the specification may be replaced by an alternative feature serving a same, equivalent, or similar purpose. Thus, unless expressly stated otherwise, disclosed features (e.g., antibodies) are an example of a genus of equivalent or similar features.

As used herein, all numerical values or numerical ranges include integers within such ranges and fractions of the values or the integers within ranges unless the context clearly indicates otherwise. Further, when a listing of values is described herein (e.g., about 50%, 60%, 70%, 80%, 85% or 86%) the listing includes all intermediate and fractional values thereof (e.g., 54%, 85.4%). Thus, to illustrate, reference to 80% or more identity, includes 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94% etc., as well as 81.1%, 81.2%, 81.3%, 81.4%, 81.5%, etc., 82.1%, 82.2%, 82.3%, 82.4%, 82.5%, etc., and so forth.

Reference to an integer with more (greater) or less than includes any number greater or less than the reference number, respectively. Thus, for example, a reference to less than 100, includes 99, 98, 97, etc. all the way down to the number one (1); and less than 10, includes 9, 8, 7, etc. all the way down to the number one (1).

As used herein, all numerical values or ranges include fractions of the values and integers within such ranges and fractions of the integers within such ranges unless the context clearly indicates otherwise. Thus, to illustrate, reference to a numerical range, such as 1-10 includes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, as well as 1.1, 1.2, 1.3, 1.4, 1.5, etc., and so forth. Reference to a range of 1-50 therefore includes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, etc., up to and including 50, as well as 1.1, 1.2, 1.3, 1.4, 1.5, etc., 2.1, 2.2, 2.3, 2.4, 2.5, etc., and so forth.

Reference to a series of ranges includes ranges which combine the values of the boundaries of different ranges within the series. Thus, to illustrate reference to a series of ranges, for example, of 1-10, 10-20, 20-30, 30-40, 40-50, 50-60, 60-75, 75-100, 100-150, 150-200, 200-250, 250-300, 300-400, 400-500, 500-750, 750-1,000, 1,000-1,500, 1,500-2,000, 2,000-2,500, 2,500-3,000, 3,000-3,500, 3,500-4,000, 4,000-4,500, 4,500-5,000, 5,500-6,000, 6,000-7,000, 7,000-8,000, or 8,000-9,000, includes ranges of 10-50, 50-100, 100-1,000, 1,000-3,000, 2,000-4,000, etc.

Modifications can be made to the foregoing without departing from the basic aspects of the technology. Although the technology has been described in substantial detail with reference to one or more specific embodiments, those of ordinary skill in the art will recognize that changes can be made to the embodiments specifically disclosed in this application, yet these modifications and improvements are within the scope and spirit of the technology.

The disclosure is generally disclosed herein using affirmative language to describe the numerous embodiments and aspects. The disclosure also specifically includes embodiments in which particular subject matter is excluded, in full or in part, such as substances or materials, method steps and conditions, protocols, or procedures. For example, in certain embodiments or aspects of the disclosure, materials and/or method steps are excluded. Thus, even though the disclosure is generally not expressed herein in terms of what the disclosure does not include aspects that are not expressly excluded in the disclosure are nevertheless disclosed herein.

The technology illustratively described herein suitably can be practiced in the absence of any element(s) not specifically disclosed herein. Thus, for example, in each instance herein any of the terms “comprising,” “consisting essentially of,” and “consisting of” can be replaced with either of the other two terms. The terms and expressions which have been employed are used as terms of description and not of limitation and use of such terms and expressions do not exclude any equivalents of the features shown and described or segments thereof, and various modifications are possible within the scope of the technology claimed. The term “a” or “an” can refer to one of or a plurality of the elements it modifies (e.g., “a reagent” can mean one or more reagents) unless it is contextually clear either one of the elements or more than one of the elements is described. The term “about” as used herein refers to a value within 10% of the underlying parameter (i.e., plus or minus 10%), and use of the term “about” at the beginning of a string of values modifies each of the values (i.e., “about 1, 2 and 3” refers to about 1, about 2 and about 3). For example, a weight of “about 100 grams” can include weights between 90 grams and 110 grams. The term “substantially” as used herein refers to a value modifier meaning “at least 95%”, “at least 96%”, “at least 97%”, “at least 98%”, or “at least 99%” and may include 100%. For example, a composition that is substantially free of X, may include less than 5%, less than 4%, less than 3%, less than 2%, or less than 1% of X, and/or X may be absent or undetectable in the composition.

Thus, it should be understood that although the present technology has been specifically disclosed by representative embodiments and optional features, modification and variation of the concepts herein disclosed can be resorted to by those skilled in the art, and such modifications and variations are considered within the scope of this technology.

Methods Ethics, Sample Processing and Flow Cytometry

The Southampton and South West Hampshire Research Ethics approved the study, and written informed consent was obtained from all subjects. Newly diagnosed, untreated patients with respiratory malignancies or HNSCC were prospectively recruited once referred. Freshly resected tumor tissue and, where available, matched adjacent non-tumor tissue was obtained from lung cancer patients following surgical resection. Samples were processed as described previously^(70,72). For sorting of CTLs, cells were first incubated with 4° C. FcR block (Miltenyi Biotec) for 10 min, then stained with a mixture of the following antibodies: anti-CD45-FITC (HI30; BioLegend), anti-CD4-PE (RPA-T4; BD Biosciences), anti-CD3-APC-Cy7 (SK7; BioLegend), anti-CD8A-PerCP-Cy5.5 (cSK1; BD Biosciences), and anti-CD103-APC (Ber-ACT8; Biolegend) for 30 min at 4° C. Live/dead discrimination was by DAPI staining CTLS were sorted based on CD103 expression using a BD FACSAria (BD Biosciences) into ice-cold TRIzol LS reagent (Ambion). HNSCC tumors were macroscopically dissected and slowly frozen in 90% FBS and 10% DMSO (Sigma) for storage until samples could be prepared.

For single-cell transcriptomic, stimulation assays, and phenotypic characterization, tumor and lung samples were first dispersed and cryopreserved in freezing media (50% complete RMPI (Gibco), 40% human decomplemented AB serum, 10% DMSO (both Sigma). Cryopreserved samples were thawed prior to staining with a combination of anti-CD45-AlexaFluor700 (HI30; BioLegend); anti-CD3-APC-Cy7 (SK7; Biolegend); anti-CD8A-PerCP-Cy5.5 (SK1; Biolegend); anti-CD103-Pe-Cy7 (Ber-ACT8; Biolegend); CD19/20 (HIB19/2H7; Biolegend); CD14 (HCD14; Biolegend); CD56 (HCD56; Biolegend) and CD4 (OKT4; Biolegend) for flow cytometric analysis and sorting. Live and dead cells were discriminated using propidium iodide (PI). For 10× single-cell transcriptomic analysis (10× Genomics), 1500 cells each of CD103⁺and CD103⁻ CTLs from tumor and lung samples were sorted and mixed into 50% ice cold PBS, 50% FBS (Sigma) on a BD Aria III or Fusion cell sorter. CTLs for assessments of the bulk transcriptome following stimulation, was collected by sorting 200 cells into 8 μL lysis buffer on an Aria Fusion (BD); for Smart-seq2-based single-cell analysis, CTLs were sorted as above using single cell purity into 4 μL lysis buffer on a BD Aria III as described.

For tumor TRM phenotyping, samples were analyzed on a FACS fusion (BD) following staining with anti-CD45-AlexaFluor700 (HI30; BioLegend); anti-CD3-APC-Cy7 (SK7; Biolegend); anti-CD8A-PerCP-Cy5.5 (SK1; Biolegend); anti-CD103-Pe-Cy7 (Ber-ACT8; Biolegend); CD127-APC (eBioRDR5; eBioscience); anti-CD39-BB515 (TU66; BD); anti-41BB-PE (4B4-1; Biolegend), anti-PD1-BV421 (EH12.1; BD); anti-TIM3-BV605 (F38-2E2; Biolegend). Cells were counter stained with CD19/20 (HIB19/2H7; Biolegend), CD14 (HCD14; Biolegend), CD56 (HCD56; Biolegend) and CD4 (OKT4; Biolegend). Dead cells were discriminated using PI. Phenotypic characterization of lung TRM was completed using the antibodies above with anti-CD49A-PE (SR84; BD) and anti-KLRG1-APC (2F1/KLRG; Biolegend) on a BD LSRII. Data was analyzed in Flowjo 10.4.1, and geometric-mean florescence intensity and population percentage data were exported and visualized in Graphpad Prism (7.0a; Treestar). For tSNE and co-expression analysis of flow cytometry data, each sample was down-sampled to exactly 3,000 randomly selected live and singlet-gated, CD19⁻CD20⁻CD14⁻CD4⁻CD56⁻CD45⁺CD3⁺CD8⁺ CTLs using the gating strategy described above, and 24,000 cells each from the lung and tumor samples were merged to yield 48,000 total cells. A tSNE plot was constructed using 1,000 permutations and default settings in Flowjo 10.4.1, z-score expression was mean centered. Flow cytometry data was exported from FlowJo (using the channel values) and these data were imported into R for co-expression analysis (described below).

Bulk-RNA Sequencing and TCR-Seq

Total RNA was purified using a miRNAeasy kit (Qiagen) from CD103⁺and CD103⁻ CTLs and was quantified as described previously^(70,72). For assessment of the stimulated transcriptome, RNA from ˜100 sorted cells was used. Total RNA was amplified according to the Smart-seq2 protocol. cDNA was purified using AMPure XP beads (0.9:1 ratio, Beckman Coulter). From this step, 1 ng of cDNA was used to prepare a standard Nextera XT sequencing library (Nextera XT DNA sample preparation and index kits, Illumina). Samples were sequenced using an Illumina HiSeq2500 to obtain 50-bp single-end reads. For quality control, steps were included to determine total RNA quality and quantity, the optimal number of PCR pre-amplification cycles, and cDNA fragment size. Samples that failed quality control or had a low number of starting cells were eliminated from further sequencing and analysis. TCR-seq was performed as previously described³¹, using Tru-seq single indexes (Illumina). Sequencing data was mapped and analyzed using MIGEC software with default settings, followed by V(D)J tools with default settings. Mapping QC matrices are included in (Table 6).

10× Single-Cell RNA Sequencing

Samples were processed using 10×v2 chemistry as per manufacturer's recommendations; 11 and 12 cycles were used for cDNA amplification and library preparation respectively. Barcoded RNA was collected and processed following manufacturer recommendations, as described previously. Libraries were sequenced on a HiSeq4000 (Illumina) to obtain 100- and 32-bp paired-end reads using the following read length: read 1, 26 cycles; read 2, 98 cycles; and i7 index, 8 cycles. Samples were pooled together DNA samples from whole blood were extracted using a High salt method and were quantified using the Qubit 2.0 (Thermo). Genotyping was completed through the Infinium Multi-Ethnic Global-8 Kit (Illumina), following the manufacturer's instructions. Raw data from the genotyping analysis was exported using Genotyping module and Plug-in PLINK Input Report Plug-in (v2.1.4) from GenomeStudio v2.0.4 (Illumina). The data quality was assessed using the snpQC package with R and low-quality SNPs were detected: SNPs failing in more than 5% of the samples and SNPs with Illumina's GC scores less than 0.2 in more than 10% of the samples were flagged. Subjects' sex was matched with the genotype data and flagged SNPs were removed for downstream analysis using PLINK (v1.90b3w). Genetic multiplexing of barcoded single-cell RNA-seq was completed using Demuxlet and matched with the Seurat output. Cells with ambiguous or doublet identification were removed from analysis of cluster and/or donor proportions.

Bulk-RNA-Seq Analysis

Bulk RNA-Seq data were mapped against the hg19 reference using TopHat (v2.0.9 (--library-type fr-unstranded --no-coverage-search) and htseq-count -m union -s no -t exon gene_name (part of the HTSeq framework, version 0.7.1)). Trimmomatic (0.36) was used to remove adapters. Values throughout are displayed as log₂ TPM (transcripts per million); a value of 1 was added prior to log transformation. To identify genes expressed differentially by various cell types, negative binomial tests for paired comparisons by employing the Bioconductor package DESeq2 (1.14.1) were performed, disabling the default options for independent filtering and Cooks cutoff. The Applicants considered genes to be expressed differentially by any comparison when the DESeq2 analysis resulted in a Benjamini-Hochberg-adjusted P value of <0.05 and a fold change of at least 2. Union gene signatures were calculated using the online tool jVenn, of which genes must have common directionality. GSEA, correlations, and heatmaps were generated as previously described^(31,72) For the preservation of complementary signatures, data from Cheuk, et al 2017 was downloaded from code GSE83637 and differential expressed was completed as above, for the murine composite signature, orthologues between human and murine signatures were compared using Biomart. Reactome pathways were generated using the online tool for tumor T_(RM)-specific genes, a pathway was considered significantly different if the FDR (q) values was <0.05 (Table 5). Visualizations were generated in ggplot2 using custom scripts, while expression values were calculated using Graphpad Prism? (7.0a). For tSNE analysis, the data frame was filtered to genes with >1 TPM expression in at least one condition and visualizations created using the top 2000 most variable genes, as calculated in DESeq2 (1.18.1); this allowed for unbiased visualization of the Log₂ (TPM+1) data, using package Rtsne (0.13). Co-expression networks were generated in gplots (3.0.1) using the heatmap2 function, while weighted correlation analysis was completed using WGCNA (1.61) from the Log₂ (TPM+1) data matrix and the function exportNetworkToCytoscape at Beta=5, weighted=true, threshold=0.05. Networks were generated in Gephi (0.92) using Fruchterman Reingold and Noverlap functions. The size and color were scaled according to the Average Degree as calculated in Gephi, while the edge width was scaled according to the WGCNA edge weight value. The statistical analysis of the overlap between gene sets was calculated in R (v3.5.0) using the fisher.test function (Stats—v3.5.0) using the number of total quantified genes used for DESeq2, as the total value, with alternative=“greater”.

Single-cell RNA-Seq analysis Raw 10× data was processed as previously described³¹, merging multiple sequencing runs using cellranger count function in cell ranger, then merging multiple cell types with cell ranger aggr. The merged data was transferred to the R statistical environment for analysis using the package Seurat (v2.2.1). Only cells expressing more than 200 genes and genes expressed in at least 3 cells were included in the analysis. The data was then log-normalized and scaled per cell and variable genes were detected. Transcriptomic data from each cell was then further normalized by the number of UMI-detected and mitochondrial genes. A principal component analysis was then run on variable genes, and the first 8 principal components (PCs) were selected for further analyses based on the standard deviation of PCs, as determined by an elbow plot in Seurat. Cells were clustered using the FindClusters function in Seurat with default settings, resolution=0.6 and 8 PCs. Differential expression between clusters was determined by converting the data to CPM and analyzing cluster specific differences using MAST (q<0.01). A gene was considered significantly different, only if the gene was commonly positively enriched in every comparison for a singular cluster³¹. Further visualizations of exported normalized data were generated using the Seurat package and custom R scripts. Cell-state hierarchy maps were generated using Monocle version 2.6.1³⁰ and default settings, including the most variable genes identified in Seurat for consistency. Average expression across a cell cluster was calculated using the AverageExpression function, and downsampling was achieved using the SubsetData function (both in Seurat). Distance between clusters was calculated by calculating a particular cells location in PCA space (Principle component 1:3) using the function GetCellembeddings (in Seurat), the values for each cell were then scaled per column (Scale function, core R) where described, and finally a distance matrix was calculated (dist function, core R, method=euclidean). This matrix was filtered to the cells assigned to cluster 1, and the mean distance of each cell in cluster 1 to all cells in each of the remaining TRM clusters (2,3,4,5) was calculated. The clustering analysis was completed using the hclust function in R (stats, R v3.5.0) with average linkage and generated from the spearman correlation analysis of each cell's location in PCA space (as above). SAVER co-expression analysis was completed on the raw-UMI counts of the TRM cells (clusters 1-5) and the non-TRM cells (remaining cells) using the function saver (v1.1.1) with pred.genes.only=TRUE, estimates.only=FALSE on transcripts assigned as uniquely enriched in cluster 2, removing genes not expressed in any cells in the non-TRM compartment. Correlation values were isolated using the cor.genes function in SAVER and co-expression plots generated as described above. Smart-seq2 single cell analysis was completed as previously described using TraCer and custom scripts to identify αβ chains and to remove cells with low QC values as previously described. Here, cells with fewer than 200,000 reads and lesser than 30% of sequenced bases assigned to mRNA were removed. Samples were mapped as described for the bulk population analysis, and the data was log transformed and displayed as normalized TPM counts; a value of 1 was added to low or zero values prior to log transformation. Visualizations were completed in ggplot2, Prism v7 (as above) and custom scripts in TraCer. A cell was considered expanded when both the most highly expressed α and β TCR chain sequences matched other cells with the same criteria. Cells were considered not expanded when neither a or 13 TCR chain sequences matched those of any other cells. A cell was considered TIM3⁺when the expression of HAVCR2 was greater than 10 TPM, while a cell was considered cycling if expression of cell cycle genes TOP2A and/or MKI67 was greater than 10 TPM. Differential expression profiling was completed using MAST (q<0.05) as previously described³¹.

Matched flow cytometry data was analyzed using FlowJo v10.4.1, values and gates were exported into ggplot and “in-silico gates” were applied using custom scripts in R. Given ˜85% of the CD103+ cells were TIM-3+ from the flow cytometry data, cells were broadly classified into TRM or non-TRM based on an individual cell's protein expression (FACS gating). Where there was no available cell-specific associated protein data, CD3+ T cells were classified based on the lack of expression of CD4 and FOXP3, to remove CD4+ cells. Next, the single cell transcriptomes were stratified into TRM or non-TRM cells when expression of TRM associated genes, ITGAE (CD103), RBPJ and/or ZNF683 (HOBIT) were greater than 10 TRM counts. Differential gene expression analysis was completed as above.

Multiplex Immunohistochemistry

Patients included in this cohort had a known diagnosis of lung cancer. 23 patients were selected in total, categorizing the donors using criteria previously reported9. A multiplexed IHC method was utilized for repeated staining of a single paraffin-embedded tissue slide. Deparaffinisation, rehydration, antigen retrieval and IHC staining was carried out using a Dako PT Link Autostainer. Antigen retrieval was performed using the EnVision FLEX Target Retrieval Solution, High pH (Agilent Dako) for all antibodies. The slide was first stained with a standard primary antibody followed by an appropriate biotin-linked secondary antibody and horseradish peroxidase (HRP)-conjugated streptavidin to amplify the signal. Peroxidase labelled compounds were revealed using 3-amino-9-ethylcarbazole (AEC), an aqueous substrate that results in red staining, or DAB that results in brown staining, and counter stained using hematoxylin (blue).

The slides were stained initially with Cytokeratin (pre-diluted, Clone AE1/AE3; Agilent Dako) then sequentially with anti-CD8a (pre-diluted Kit IR62361-2; clone C8/144B; Agilent Dako), anti-CD103 (1:500; EPR4166(2); abcam) and anti-TIM-3 (1:50; D5D5R; Cell Signaling Technology). The slides were scanned at high resolution using a Zeiss Axio Scan.Z1 with a 20× air immersion objective. Between each staining iteration, antigen retrieval was performed along with removal of the labile AEC staining and denaturation of the preceding antibodies using a set of organic solvent based de-staining buffers; 50% ethanol for 2 minutes; 100% ethanol for 2 minutes; 100% xylene for 2 minutes; 100% ethanol for 2 minutes; 50% ethanol for 2 minutes. This process did not affect DAB staining. The process was repeated for each of the antibodies.

Bright field images were separated into color channels in imaging processing software ImageJ FIJI81 (ImageJ Windows 64-bit final version). For the TIL^(high)TRM^(high) and TIL^(low)TRM^(low) tumors the number of CD8+CD103+TIM3+ cells were quantified manually. Two samples with ≤3 CD8+CD103+ CTLs quantified were removed, to prevent calculating percentages of single events, resulting in a final number of 21 samples. These images were processed and combined to create pseudo-color multiplexed images. The raw counts for each protein, individually and together are presented in Table 7, as the number of cells per 0.15 mm2.

OMNI-ATAC-Seq

CTLs were FACS sorted from cryopreserved lung cancer samples as described above, using the following antibody cocktail: anti-CD45-AlexaFluor700 (HI30; BioLegend); anti-CD3-APC-Cy7 (SK7; BioLegend); anti-CD8A-PerCP-Cy5.5 (SK1; BioLegend); anti-CD103-Pe-Cy7 (Ber-ACT8; BioLegend); anti-CD127-APC (eBioRDR5; ThermoFisher); anti-TIM-3-BV605 (F38-2E2; BioLegend). Cells were counter stained with anti-CD19/20-PEDazzle (HIB19/2H7; BioLegend); anti-CD14-PE-Dazzle (HCD14; BioLegend); and anti-CD4-BV510 (OKT4; BioLegend). Dead cells were discriminated using PI. Samples were sorted into low retention 1.5 ml eppendorfs containing 250 μL FBS and 250 μL PBS. Three to six donors were pooled together to guarantee sufficient cell numbers. For each pool of cells, two or three technical replicates of 15,000-25,000 CTLs were generated for each library.

OMNI-ATAC-seq was performed as described in Corces, et al., with minor modifications. Isolated nuclei were incubated with tagmentation mix (2×TD buffer, 2.5 μL transposase enzyme from Nextera kit, Illuminia) at 37° C. for 30 minutes in a thermomixer, shaking at 1000 RPM. Following tagmentation, the product was eluted in 0.1× Tris-EDTA buffer using DNA Clean and Concentrator-5 kit (Zymo). The Purified product was preamplified for 5 cycles using Kappa 2× enzyme along with Nextera indexes (Illumina) and based on qPCR amplification, an additional 7 cycles of amplification was performed for 20,000 cells. The PCR amplified product was purified using DNA Clean and Concentrator-5 kit (Zymo), and size selection was done using AMPure XP beads (Beckman Coulter). Finally, concentration and quality of libraries were determined by picogreen and bioAnalyzer assays. Equimolar libraries were sequenced as above, or on a NovaSeq 6000 for sequencing.

Next, technical replicates were randomly down sampled to between 25,000,000 to 40,000,000 total reads and merged using Bash scripts, resulting in two TIM-3+IL-7R-TRM pools and two non-TRM pools. These reads were mapped to hg19 with bowtie2 (v2.3.3.1). Chromosomes 1-22, and X were retained, chrY, chrM, and other arbitrary chromosome information based reads were removed. Samtools (v1.9) was used to get the uniquely mappable reads, only reads MAPQ≥30 were considered. Duplicate reads are removed by “MarkDuplicates” utility of Picard tool (v 2.18.14). Before peak calling, tag align files were created, by shifting forward strands by 4 bases, and reverse strands by 5 bases (TN5 shift). Peaks were identified with MACS2 (v 2.1.1.20160309) using the function. -f BED -g ‘hs’-q 0.01 --nomodel --nolambda --keep-dup all --shift -100 --extsize 200. BamCoverage (v2.4.2) was used for converting bam files into bigwig, and further UCSC track generation (same normalization across all ATACseq and RNAseq samples), as per the following example: bamCoverage -b TIL_103 pos.bam -o TIL_103 pos_NormCov.bw -of bigwig -bs 10 --normalizeTo1x 2864785220 --normalizeUsingRPKM -e 200. The R package DiffBind (v2.2.12) was used to highlight differentially accessible peaks (based on DEseq2). R packages of org.Hs.eg.db (v3.4.0 and TxDb.Hsapiens.UCSC.hg19.knownGene (v3.2.2) were used to annotate peaks. Following differential expression peaks were filtered to those within 5 kb of a transcription start site to focus directly on promoter accessibility. The correlation plot (spearman) was completed as described above, using all identified peaks. The plot was clustered according to complete linkage.

Statistical Analysis

The significance of differences among matched samples were determined by Wilcoxon matched-pairs signed rank test unless otherwise stated. Statistical analyses were performed using Graphpad Prism? (7.0a). Spearman correlation coefficient (r value) was used to access significance of correlations between the levels of any two components of interest.

Data Availability

Sequencing data was deposited into the Gene Expression Omnibus.

Immunotherapy is rapidly becoming a mainstream treatment of solid cancers^([51,52]); nonetheless, less than 30% of patients benefit from this approach^([53]). Thus, there is an urgent need to develop novel immunotherapeutic agents for the patients who do not respond to currently available immunotherapies. Applicants' goal is to identify such novel targets by systematically investigating the molecular mechanisms that drive the development and function of a novel class of cytotoxic T lymphocytes (CTLs) in the tumor immune microenvironment (TIME)—tissue-resident memory cells (T_(RM)), which Applicants have recently shown to be key players in driving effective anti-tumor immune responses in lung cancer^([54]). This breakthrough finding (Nature Immunology 2017) was possible because of the ongoing collaboration between the Applicants.

Tissue-resident memory (Tam) CTLs in cancer: Applicants were the first to conclusively show that higher density of T_(RM) cells in tumor tissue (defined here as ‘immune hot’ tumors) predicted better survival outcomes in human cancers, and that this effect was independent of that conferred by the density of the global CD8⁺ T cell population in tumors^([101]) (FIG. 49). To understand the molecular features that drive efficient T_(RM) immune responses in the TIME, Applicants performed single-cell and bulk RNA-Seq analysis of purified populations of TRM and non-T_(RM) cells present in tumor and normal lung tissue from lung cancer patients. The key results were: (i) The identification of a novel TIM-3 expressing TRM subset present exclusively in tumors. This subset also expressed high levels of PD-1. Surprisingly, however, they proliferated in the tumor milieu, released effector cytokines when stimulated ex-vivo and exhibited a transcriptional program indicative of superior effector, survival and tissue residency properties (FIG. 2-FIG. 4). This ‘highly functional’ PD-1+TIM-3+T_(RM) subset was validated by functional assays ex-vivo and reflected in the chromatin accessibility profile of this subset. This TIM-3+IL-7R-TRM subset was enriched in responders to PD-1 inhibitors and in tumors with a greater magnitude of CTL responses. These data highlights that not all CTLs expressing PD-1+ are dysfunctional, in particular, TRM cells with the highest PD-1 expression are enriched for features of superior functionality. (ii) Definition of a core set of genes that were enriched in the ‘highly functional’ PD-1⁺TIM-3⁺ T_(RM) subset in tumors, which included a number of novel genes (e.g., AMICA, SIPRG, KIR2DL4) whose expression was highly correlated with known tissue residency (T_(RM)) genes. Any of these genes are likely to be critically important for the development, trafficking or function of tumor-infiltrating TRM cells. (iii) M1^(hot) myeloid cells in the TIME were associated with robust TRM anti-tumor responses. This finding was revealed by Applicants' novel integrated weighted gene correlation network analysis (iWGCNA) analysis of matched CTLs and myeloid cells.

Applicants hypothesize, without being limited to a particular theory: (i) ‘Highly functional’ PD-1⁺TIM-3⁺ T_(RM) subset are increased in numbers and qualitatively superior in patients with ‘immune hot’ tumors and in ‘responders’ to anti-PD1 therapy. (ii) Expansion of this TRM subset in ‘immune hot’ tumors is positively correlated with expansion of myeloid subsets (M1^(hot)) that promote anti-tumor immunity. (iii) ‘Candidate molecules’ (AMICA, SIRPG, CD38 etc.,) whose expression is enriched in ‘highly functional’ TRM cells are promising immunotherapy targets to boost anti-tumor TRM responses.

Results

The identification of molecular players and pathways that lead to the generation of effective anti-tumor TRM immune responses will inform the discovery of new drug targets for treating cancer. Current knowledge of these players is vastly incomplete, as investigative studies are mainly focused on genes and molecules identified based on a priori concepts in immunology and cell biology and have thus far neglected the study of tumor-infiltrating TRM cells. Applicants' team performed the first and largest unbiased survey of bulk and single-cell transcriptomes from purified TRM CTLs isolated from tumors of patients with cancer.

T_(RM) CTL responses have also recently been shown by Applicants⁹ and others¹⁰ to be associated with better survival in patients with solid tumors. The molecular features of TRM cells' responses have been characterized in infection models, and involve rapid clonal expansion and upregulation of molecules aiding recruitment and activation of additional immune cells alongside the conventional effector functions of CTLs¹¹. To date, the properties of TRM cells found in the background lung, compared to those in the tumor are not fully elucidated. Furthermore, the properties of these cell subsets in the context of immunotherapy are still poorly understood. To address this question, Applicants compared the transcriptome of TRM and non-T_(RM) CTLs present in tumor and normal lung tissue samples from treatment naïve patients with lung cancer. Furthermore, Applicants investigated the same tissue resident populations in head and neck squamous cell carcinoma and during immunotherapy regimes. Key results are summarized below:

Shared Features of TRM Cells in Human Lungs and Tumor.

Applicants compared the transcriptome of CTLs isolated from lung tumor and adjacent uninvolved lung tissue samples obtained from patients (n=30) with treatment-naïve lung cancer, sorted according to CD103 expression to separate TRM from non-T_(RM) cells. Lung CD103⁺ and CD103⁻ CTLs clustered separately and showed differential expression of nearly 700 transcripts including several previously linked to TRM phenotypes (FIG. 2). These data confirm that CD103⁺ CTLs in human lungs and tumors are highly enriched for TRM cells; hereafter Applicants refer to CD103⁺ CTLs as TRM cells and CD103⁻ CTLs as non-T_(RM) cells. Applicants next asked if TRM cells in lung tumors share tissue residency features with TRM cells in adjacent normal lung tissue. Nearly one-third (89/306) of the TRM properties, i.e., transcripts differentially expressed between CD103⁺and CD103⁻ CTLs in tumors that were shared with those of normal lung TRM cells (FIG. 2C, venn diagram). Weighted gene co-expression network analysis (WGCNA) of the 89 ‘shared tissue residency’ transcripts revealed a number of novel genes whose expression was highly correlated with known tissue residency genes (S1PR1, S1PR5, ITGA1, HOBIT, RBPJ^(12,13)), suggesting that their products may also play important roles in the development, trafficking or function of T_(RM) cells (FIG. 2E). Notable examples encoding products likely to be involved in T_(RM) functionality, migration or retention include SRGAP3¹⁷, AMICA1¹⁸, CAPG¹⁹, ADAM19²⁰, and NUAK2²¹ (FIG. 2E).

PD-1 Expression is a Feature of Tumor and Lung TRM Cells.

Another important ‘shared tissue residency’ transcript was PDCD1, encoding PD-1 (FIG. 2E). Although PD-1 expression is considered typical of exhausted T cells as well as activated cells³, recent reports have suggested that high PD-1 expression is a tissue residency feature of brain TRM cells independent of antigen stimulation^(22,23), and of murine T_(RM) cells from multiple organ systems¹⁴. In support of the conclusion that high expression of PD-1 reflects tissue residency, rather than exhaustion, Applicants found that when T_(RM) and non-T_(RM) cells isolated from both lung and tumor tissue were stimulated ex vivo, they showed robust up-regulation of TCR-activation-induced genes and cytokines (TNF, IFNG) (data not shown). In addition to PDCD1, ‘shared tissue-residency’ transcripts included several (SPRY1²⁴, TMIGD2²⁵, CLNK²⁶) that encode products reported to play a regulatory role in other immune cell types (FIG. 2E). Applicants speculate that the expression of these inhibitory molecules may restrain the functional activity of tumor TRM cells and may represent targets for future immunotherapies.

Tumor TRM Cells were Clonally Expanded, Proliferate and Express Markers of Enhanced Function.

To identify features unique to tumor TRM cells, Applicants compared the transcriptome of TRM cells and non-T_(RM) cells from both normal lung and tumors and detected 93 differentially expressed transcripts (FIG. 3A) specifically in this subset, hence termed ‘tumor T_(RM)-enriched’ transcripts. Reactome pathway analysis of these ‘tumor T_(RM)-enriched’ transcripts showed significant enrichment for transcripts encoding components of the canonical cell cycle, mitosis and DNA replication machinery (FIG. 3B). The tumor TRM subset thus appears to be highly enriched for proliferating CTLs, presumably responding to tumor-associated antigens (TAA), despite PD-1 expression. Unique molecular identifier (UMI)-based T cell receptor (TCR) sequencing assays revealed that TRM cells in tumors expressed a significantly more restricted TCR repertoire than non-TRM cells in tumors. Furthermore, the tumor TRM population contained a higher mean percentage of expanded clonotypes (73% versus. 52%, in tumor TRM versus. non-T_(RM) populations, data not shown).

‘Tumor T_(RM)-enriched’ transcripts that were highly correlated with cell cycle genes may encode products with important functions, as they are likely to reflect the molecular features of TRM cells that are actively expanding in response to TAA. HAVCR2, encoding the co-inhibitory checkpoint molecule TIM-3, was most correlated and connected with cell cycle genes (FIG. 3E). Thus, TIM-3 expression may be a feature of lung tumor TRM cells that is not linked to exhaustion, but rather reflects a state of ‘high functionality, as the other transcripts that correlated with expression of TIM-3 and cell cycle genes encode molecules that likely confer superior functionality, such as CD39 (encoded by ENTPD1)³⁰, CXCL13³¹, CCL3³², TNFSF4³³ (OX-40 ligand), as well as a marker of antigen-specific engagement (4-1BB)³⁴ (FIG. 3E). Robust expression of this set of molecules was not observed in either human lung TRM cells or in the mouse TRM signatures^(13,14,35), indicating that the tumor TRM population contains novel cell subsets.

Single-Cell Transcriptomic Analysis Reveals Previously Uncharacterized TRM Subsets.

To determine whether ‘tumor T_(RM)-enriched’ transcripts are expressed in all or only a subset of the tumor TRM population, Applicants performed low resolution (10× platform) single-cell RNA-seq assays in CD103⁺and CD103⁻ CTLs isolated from tumor and matched adjacent normal lung tissue from 12 patients with early-stage lung cancer. Analysis of the ˜12,000 single-cell transcriptomes revealed 5 clusters of TRM cells and 4 clusters of non-T_(RM) cells (FIG. 4A, FIG. 4B). Among the 5 TRM clusters, clusters 1-3 contained a greater proportion of the tumor TRM population while clusters 4 and 5 contained more lung TRM cells (FIG. 4C). Most strikingly, clusters 1-3 contained very few lung TRM cells (FIG. 4C). Applicants infer that the ‘tumor T_(RM)-enriched’ transcripts detected in Applicants' analysis of bulk populations were likely to be contributed by cells in these subsets. In agreement with that conclusion, cells in cluster 1 expressed high levels of the 25 cell cycle-related ‘tumor T_(RM)-enriched’ transcripts³⁶, indicating that the enrichment of cell cycle transcripts in the bulk tumor TRM population was contributed by this relatively small subset. Because these cells are actively proliferating, they likely represent TAA-specific cells. The majority of cells in this cycling cluster were from the tumor TRM population. These cells, as well as those in the larger cluster 2, were highly enriched for other prominent ‘tumor Tom-enriched’ transcripts like HAVCR2 (TIM-3), including those encoding products that could confer superior functionality (e.g., CD39⁹, CXCL13³¹, CCL3³²), consistent with recent reports^(28,29). This shared expression pattern suggests that the cycling cluster (cluster 1) may simply represent cells in cluster 2 that are entering the cell cycle. Confirming this idea, cell-state hierarchy maps of all TRM cells, constructed using Monocle2³⁷, revealed that cells in cluster 2 were most similar to the cycling TRM cells (cluster 1, data not shown). Overall, Applicants' single-cell transcriptome uncovered additional phenotypically distinct subsets of tumor TRM cells that have not previously been described and are likely to play an important role in anti-tumor immune responses.

TIM-3⁺IL7R⁻ TRM Subset has a Transcriptional Program Indicative of Superior Functional Properties.

Because of their close relationship with cycling TRM cells, Applicants focused Applicants' analysis on the TRM cells in cluster 2. The 91 transcripts enriched in cluster 2 compared to the other TRM clusters included several which encoded products linked to cytotoxic activity such as PRF1, GZMB, GZMA, CTSW³⁸, and CRTAM³⁸, as well as transcripts encoding effector cytokines and chemokines such as IFNG, CCL3, CXCL13, IL17A and IL26 (FIG. 5C and data not shown). Cluster 2 also expressed high levels of transcripts encoding transcription factors known to promote the survival of memory or effector CTLs (ID2⁴⁵, STAT3⁴⁶, ZEB2⁴⁷) or those that are involved in establishing and maintaining tissue residency (RBPJ, a key player in Notch signaling¹³, and BLIMP1³⁵, encoded by PRDM1). TRM cells in cluster 2 also strongly expressed ENTPD1, which encodes CD39, an ectonucleotidase that cleaves ATP, which may protect this TRM subset from ATP-induced cell death in the ATP-rich tumor microenvironment³⁰ and has recently been shown to be enriched for tumor neo-antigen specific CTLs^(49,50). This expression pattern likely confers highly effective anti-tumor immune function; in combination with earlier results, Applicants conclude that this ‘highly functional TIM-3⁺IL7R⁻ ^(TRM) subset’ likely represents TAA-specific cells that were enriched for transcripts linked to cytotoxicity.

Intriguingly, TRM cells in cluster 2 (TIM-3⁺IL7R⁻ subset) expressed the highest levels of PDCD1 transcripts and were enriched for transcripts encoding other molecules linked to inhibitory functions such as TIM-3, TIGIT⁵¹, and CTLA4⁵²⁻⁵⁴. Nonetheless, these TRM cells exhibited a transcriptional program suggestive of superior effector properties and cell proliferation expressed high transcript levels for cytotoxicity molecules (Perforin, Granzyme A and Granzyme B) and several co-stimulatory molecules such as 4-1BB, ICOS and GITR (TNFRSF18) (FIG. 5C and data not shown).³ More specifically, PDCD1-expressing TRM cells in cluster 2 expressed relatively higher levels of IFNG, CCL3, and CXCL13 transcripts compared with cells not expressing PDCD1 in that cluster and other tumor-infiltrating TRM and non-T_(RM) cells (FIG. 5D. Overall, these findings agree with the bulk RNA-seq analysis, indicating that expression of inhibitory molecules, like PD-1, does not reflect exhaustion. Instead, it may prevent TCR-activation-induced cell death to sustain robust anti-tumor CTL responses.

PD-1- and TIM-3-Expressing Tumor-Infiltrating TRM Cells are not Exhausted.

To further support the case that PDCD1-expressing TRM cells in cluster 2 (TIM-3⁺IL7R⁻ ‘highly functional’ TRM cells) are not exhausted, but instead highly functional, Applicants performed single-cell RNA-seq in tumor-infiltrating TRM and non-T_(RM) cells, using the more sensitive Smart-seq2 assay for paired transcriptomic and TCR clonotype analysis³⁸. As expected, clonally expanded tumor-infiltrating TRM cells, which are likely to be reactive to TAA, were significantly enriched for genes specific to ‘highly functional’ TIM-3⁺IL7R⁻ TRM cells. Among tumor-infiltrating CTLs, a greater proportion of TIM-3-expressing TRM cells were clonally expanded compared with other TRM and non-T_(RM) cells (FIG. 6A, FIG. 6B). Furthermore, TIM-3-expressing TRM cells were significantly enriched for key effector cytokines and cytotoxicity transcripts, despite expressing significantly higher levels of PDCD1 (data not shown). The higher sensitivity of the SMART-seq2 assay compared to the 10× genomics platform also allowed better co-expression analysis³⁸. Specifically, IFNG and PDCD1 expression levels were better correlated in TIM-3-expressing TRM cells compared with non-T_(RM) cells (FIG. 6D), and the proportion of cells strongly co-expressing these transcripts was notably higher (30% versus. 1%). Overall, these results strongly support that PD-1 and TIM-3 expressing tumor-infiltrating TRM cells were not exhausted, but instead were enriched for transcripts (IFNG, PRF1, GZMA) encoding for molecules linked to effector functions are “highly functional.”

Functional and Protein Validations.

In keeping with Applicants' transcriptomic assays, when stimulated ex-vivo, tumor-infiltrating TRM cells that co-expressed PD-1 (stained before stimulation) had significantly higher percentage of cells expressing effector cytokines, when compared to the non-T_(RM) CTLs that co-expressed PD-1 (FIG. 50). Analysis directly ex-vivo demonstrated there was also greater expression of cytotoxic-associated proteins, granzyme A and granzyme B, in the PD-1⁺ TRM cells when compared to the PD-1⁺non-T_(RM) CTLs in the tumor (FIG. 50). These data verify that PD-1 expression in the TRM subset of tumor-infiltrating CTLs does not reflect dysfunctional properties.

Tumor Specificity the Highly Functional Tumor TRM Cells.

TIM-3-expressing CTLs were also detected among tumor-infiltrating TRM cells isolated from both treatment naïve lung cancer and head and neck squamous cell carcinoma (HNSCC) samples, but not among non-T_(RM) cells in these treatment naïve tumors or TRM cells in lung. These finding confirmed, at the protein level, the specificity of the TIM-3⁺IL-7R⁻ TRM subset to tumors from two cancer types studied.

Applicants' bulk and single-cell transcriptomic analysis of purified population of TRM cells showed that the molecular program of tumor-infiltrating TRM cells is substantially distinct from that observed in the human background lung tissue or in murine models. The most striking discovery was the identification of a ‘highly functional’ TIM-3-expressing TRM subset present exclusively in tumors. This subset expressed high levels of PD-1 and other molecules previously thought to reflect exhaustion. Surprisingly however, they proliferated in the tumor milieu, were capable of robust up-regulation of TCR-activation-induced genes and protein expression of cytokines when stimulated ex vivo and exhibited a transcriptional program indicative of superior effector, survival and tissue residency properties. T_(RM) subsets and their molecular properties that associate with response to anti-PD1 therapy.

Analysis of CTLs from anti-PD-1 responders Applicants analysed tumor-infiltrating T cells from 19 biopsies (FIG. 54) with known divergent responses to anti-PD-1 therapy. Flow cytometry analysis of tumor TRM cells isolated from responding patients pre-, during-, and post-treatment samples showed an increased proportion of TIM-3⁺IL-7R⁻ ^(TRM) cells when compared to the tumor TRM cells from Applicants' cohort of treatment naïve lung cancer patients and those not responding to anti-PD-1 (˜70% versus. ˜24% and ˜9%, respectively; (FIG. 54, FIG. 56B, FIG. 57). Pre-anti-PD-1 therapy that was diminished post-treatment is likely reflective of the clinical antibody blocking flow cytometric analysis (FIG. 54B) Since this population also expressed high levels of PD-1 (FIG. 6F, FIG. 54B) Applicants show that these TRM cells may be the key responder cells to anti-PD-1 therapy. To comprehensively evaluate the molecular features and clonality of the CTLs (FIG. 58A, FIG. 58B) responding to anti-PD-1 therapy, Applicants performed paired single-cell transcriptomic and TCR analysis of CTLs isolated from biopsies both pre- and post-therapy from two donors. Differential expression analysis of all CD8+ tumor-infiltrating CTLs revealed a significant enrichment of markers linked to cytotoxic function (PRF1, GZMB and GZMH) and activation (CD38) in post-treatment compared to pre-treatment samples (FIG. 54C, FIG. 54D). Furthermore, Applicants found sharing of TCR clonotypes (FIG. 58C, FIG. 58D) between CTLs from post and pre-treatment samples (not shown), which suggested that tumor-infiltrating CTLs with the same specificity displayed enhanced cytotoxic properties following anti-PD-1 treatment. Notably, Applicants found increased expression of ITGAE, a marker of TRM cells, in CTLs from post-treatment samples (FIG. 54C, FIG. 54D). GSEA analysis also showed that tumor-infiltrating T cells from post-treatment samples were enriched for TRM features as well as those linked to TIM3⁺IL7R⁻ TRM subset (FIG. 54E, Table 4). Unbiased co-expression analysis of transcripts from post-treatment CTLs demonstrated that that transcripts linked to cytotoxicity (GZMH) and activation (CD38) clustered together with the TRM marker gene (ITGAE; FIG. 54F). Together, these results indicated that anti-PD-1 treatment enhanced the cytotoxic properties of tumor-infiltrating CTLs and that TRM cells largely contributed to this feature.

To provide a further line of evidence for the functional potential of TIM-3+IL-7R-TRM cells and to further characterize their epigenetic profile, Applicants performed OMNI-ATAC-seq on purified populations of tumor-infiltrating TIM3+IL7R-TRM and non-TRM subsets pooled from lung cancer patients (n=9, FIG. 7, FIG. 13). These subsets clustered separately, highlighting the distinct chromatin accessibility profiles of these populations (FIG. 54G). In keeping with transcriptomic analyses (FIG. 2D), Applicants identified greater chromatin accessibility within 5 kb of the transcriptional start site of the CD103 (ITGAE) and KLF3 loci, in the TRM and non-TRM compartment, respectively. Furthermore, consistent with single-cell transcriptional data, the TIM3+IL7R-TRM cells when compared to non-TRM cells showed increased chromatin accessibility of genes encoding effector molecules such as granzyme B and IFN-γ, despite showing increased accessibility at the PDCD1 (PD-1) and TIM-3 (HAVCR2) loci (FIG. 54H). Taken together, these epigenetic and transcriptomic data, combined with protein validation highlighted the potential functionality of the TIM-3+IL-7R-TRM cells, which positively correlated with expression of PD-1 specifically in this subset.

Based on the above findings, Applicants hypothesize, without being limited to a particular theory, that the highly functional ‘PD-1⁺TIM-3⁺ TRM subset is one of the key responder cell types to anti-PD1 therapy.

Functional Analysis of Novel Molecules Linked to TRM CTL Development and/or Function.

New molecules linked to TRM immune response: In Applicants' transcriptomic study of total CD8⁺ TILs, transcripts for molecules that have been shown to be effective immunotherapy targets such as PD-1 and TIM-3 were among the most enriched in tumors with CD8^(high) and CD103^(high) TIL status, which were both independently linked to better anti-tumor immunity and survival outcome. Therefore, Applicants reasoned that other molecules in the list of genes upregulated in tumors with CD8^(high) and CD103^(high) TIL status might also play an important functional role in modulating the magnitude and specificity of anti-tumor immune responses (FIG. 50), such as:

(i) CD38, an ectonucleotidase with various functions including regulation of adenosine signaling, adhesion, and transduction of activation and proliferation signals^([162, 163)]. Given that purinergic receptors can be therapeutically targeted, it will be pertinent to test how CD39 and CD38 modulate ATP and purinergic signaling to influence the development and function of anti-tumor TRM cells (CD103⁺CD8⁺ TILs). Applicants will test functions of these targets.

(ii) KIR2DL4, upregulated in T_(RM)-high tumors, encodes the killer cell immunoglobulin-like receptor KIR2DL4, which has activating and inhibitory functions^([164]) HLA-G, a non-classical MHC class I molecule, has been shown to engage KIR2DL4 and increase cytokine production by NK cells^([165]). Though the expression of HLA-G is highly restricted, several reports have shown its increased expression in tumor tissue, especially in lung cancer^([166]), so Applicants speculate that HLA-G in tumors may activate CTLs via the KIR2DL4 receptor to enhance their anti-tumor activities.

(iii) SIRPG encodes a member of the immunoglobulin superfamily of signal-regulatory proteins (SIRPs) that interact with the ubiquitously expressed CD47 molecule^([167]). Interestingly, SIRPG is the only member of the SIRP family that is expressed on T cells, and its interaction with CD47 expressed on APCs was shown to enhance T cell proliferation and IFN-γ production^([168]). Based on the increased expression of SIRPG transcripts in CD103^(high)CD8⁺ TILs, Applicants speculate that SIRPG may also serve as an important co-stimulatory molecule and its function could be exploited to enhance the anti-tumor function of CTLs.

More recently, Applicants performed additional studies in purified populations of TRM cells in lung and tumor tissue (FIG. 2-FIG. 5). These analyses defined a core set of genes commonly expressed in both lung and tumor TRM cells, including a number of novel genes whose expression was highly correlated with known tissue residency (T_(RM)) genes. Any of these genes may also be critically important for the development, trafficking or function of lung or lung tumor-infiltrating TRM cells. Some notable examples known or likely to have such functions are AMICA1⁶⁸, encoding JAML (junctional adhesion molecule-like), which contributes to the proliferation and cytokine release of skin-resident γδT cells; and SRGAP3, whose product functions in neuronal migration⁶⁷. Additional TRM candidate molecules that are associated with ‘immune hot’ tumors, M1^(hot) myeloid program, interferon-response signature in tumor cells, and finally responsiveness to anti-PD1 therapy will be tested.

Additionally, Applicants have validated high protein expression of AMICA1 and found heightened expression in tumor infiltrating CD8 T cells, not only substantiating the RNA-seq data, but also highlighting CD8+ TILs as cellular targets of potential immunotherapy intervention. (FIG. 52) Applicants have also validated a knockout system specifically depleting AMICA1 in tumor antigen-specific CD8 T cells. By adoptively transferring these cells into tumor-bearing recipient mice, the Applicants discovered that although AMICA-1−/−CD8 cells efficiently migrate into the tumor micro environment, they fail to facilitate efficient anti-tumor effects compared with control CD8 T cells. These data indicate that a lack of AMICA1 expression specifically in CD8+ TILs ensues loss of functionality. Additionally, B16F10-OVA tumor-bearing mice were treated with either anti-PD-1, anti-AMICA-1 or isotype control antibodies. These data, shown in FIG. 52K further corroborate the previous results by illustrating that treatment with an agonistic anti-AMICA1 antibody significantly impedes tumor growth. The combination of this finding and previous data discussed herein suggests that this effect is mediated via stimulation of tumor infiltrating CD8+ T cells.

EQUIVALENTS

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this technology belongs.

The present technology illustratively described herein may suitably be practiced in the absence of any element or elements, limitation or limitations, not specifically disclosed herein. Thus, for example, the terms “comprising,” “including,” “containing,” etc. shall be read expansively and without limitation. Additionally, the terms and expressions employed herein have been used as terms of description and not of limitation, and there is no intention in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, but it is recognized that various modifications are possible within the scope of the present technology claimed.

Thus, it should be understood that the materials, methods, and examples provided here are representative of preferred aspects, are exemplary, and are not intended as limitations on the scope of the present technology.

The present technology has been described broadly and generically herein. Each of the narrower species and sub-generic groupings falling within the generic disclosure also form part of the present technology. This includes the generic description of the present technology with a proviso or negative limitation removing any subject matter from the genus, regardless of whether or not the excised material is specifically recited herein.

In addition, where features or aspects of the present technology are described in terms of Markush groups, those skilled in the art will recognize that the present technology is also thereby described in terms of any individual member or subgroup of members of the Markush group.

All publications, patent applications, patents, and other references mentioned herein are expressly incorporated by reference in their entirety, to the same extent as if each were incorporated by reference individually. In case of conflict, the present specification, including definitions, will control.

Other aspects are set forth within the following claims.

TABLE 1 Gene AMICA1 CD28H CHN1 SPRY1 CD226 PTPN22 DUSP4 CLEC2D KRT86 CD101 CD200R1

TABLE 2 Gene AMICA (JAML) SPRY1 CHN1 PAG1 PTPN22 DUSP4 ICOS TNFRSF18 (GITR) CD28H (TMIGD2) CD226 TIGIT KLRC1 (NKG2A) KLRC2 (NKG2C) CAPG MYO1E CLEC2B (AICL - Activation-Induced C-Type Lectin) CLECL1 TNFRSF9 (4-1BB/CD137) TNFSF4 (OX-40L) NR3C1 CD7 KLRD1 (CD94) CLEC2D ITM2A VCAM1 (CD106) KRT81 KRT86 CXCL13 CBLB KLRC3 (NKG2-E) KLRB1 (CD161) CD101 CD109 CD200R1 SLA (SLAP)

TABLE 3 List of genes differentially expressed between Lung TRM compared to Lung non-TRM log2FoldChange pvalue padj Mean TPM Lung CD103neg MeanTPM Lung CD103pos ITGAE 3.688320634 1.70E−59 1.97E−55 21.46150476 265.10205 S1PR5 −6.687936244 1.10E−42 6.36E−39 134.1027238 3.67714065 CX3CR1 −6.991370416 1.82E−36 7.04E−33 175.2148667 1.804877645 S1PR1 −3.641962229 1.06E−26 3.07E−23 239.1302906 19.1754866 PRSS23 −4.083087713 3.19E−26 7.40E−23 22.8481059 1.4572142 FCRL3 −5.450452146 3.69E−24 7.13E−21 56.6788171 8.42927178 FGFBP2 −5.23625676 2.83E−20 4.68E−17 287.5229123 7.5970008 LINC00987 −4.335283635 6.93E−20 1.00E−16 11.51608357 0.60433175 ADGRG1 −3.690227626 3.02E−19 3.90E−16 146.5809238 15.91066901 ZNF683 2.840701441 2.39E−18 2.77E−15 91.602989 777.8943737 LDLRAD4 2.720609868 4.83E−17 5.09E−14 4.93274 24.7280175 PLEK −3.953518263 5.97E−17 5.77E−14 325.5233143 31.67810321 LIMD2 −1.161393759 1.27E−16 1.13E−13 182.1111619 85.68926 MIR4461 1.308404092 4.29E−16 3.55E−13 2173.675619 6586.9165 KLRAP1 −4.875420416 4.06E−15 3.14E−12 32.02053743 3.0425634 PDGFD −4.717712955 6.21E−15 4.50E−12 29.30154943 1.53936536 C1orf21 −4.088843226 1.51E−14 1.03E−11 13.63393756 0.99581828 FCGR3A −2.490323421 1.64E−14 1.06E−11 357.0966381 74.9510268 SPRY1 4.365932648 3.96E−14 2.42E−11 3.749139257 82.8237395 ABCC9 1.306250208 6.60E−14 3.83E−11 2.177677476 5.7461988 OPHN1 1.698830765 8.04E−14 4.44E−11 7.505019524 22.160505 LGR6 −4.134216295 3.75E−13 1.98E−10 27.19662095 2.625316415 SNTB2 2.065569735 4.19E−13 2.11E−10 3.784128857 13.9412381 PLAC8 −2.522266407 4.44E−13 2.15E−10 114.6815 26.4785965 LOC102724699 1.780016048 6.86E−13 3.18E−10 8.024455238 27.9874615 FEZ1 −2.934653107 1.47E−12 6.55E−10 22.35391219 3.54457915 KLRG1 −2.873819675 1.88E−12 8.09E−10 469.6674938 74.7741715 GPR155 2.047989691 3.24E−12 1.21E−09 7.462555429 24.60980145 RNF130 −2.989955573 3.08E−12 1.21E−09 110.1369376 19.8595257 SELL −3.329331038 3.22E−12 1.21E−09 507.2940325 55.0757787 KLF3 −3.67725202 3.02E−12 1.21E−09 62.23310952 8.94888858 KLRC1 3.279902899 3.76E−12 1.36E−09 36.97543338 428.0637866 MTRNR2L2 1.563127479 4.80E−12 1.64E−09 1391.110667 5314.522 ZNF365 −3.395971331 4.80E−12 1.64E−09 6.917136971 1.11013625 PREX1 2.029428202 6.66E−12 2.21E−09 17.72572143 72.19956 MTRNR2L8 1.466995835 6.86E−12 2.21E−09 544.3467143 1855.34295 ARHGEF26-AS1 1.490358206 8.70E−12 2.66E−09 4.932213333 13.310475 JUNB −1.252749633 8.64E−12 2.66E−09 1745.32781 638.31095 ITGA1 4.051013491 9.38E−12 2.78E−09 7.034438786 137.3742612 PHLDA1 1.983873635 9.82E−12 2.78E−09 25.87213667 89.10818075 RAP1GAP2 −4.254360181 9.60E−12 2.78E−09 12.79643925 0.769949395 A2M −2.434435477 1.61E−11 4.44E−09 27.18622429 5.6312523 LGALS3 1.474559911 1.91E−11 5.17E−09 541.8120429 685.16675 PLP2 1.585554018 3.40E−11 8.96E−09 133.4817429 328.37947 CLDN11 1.49888062 3.90E−11 1.01E−08 7.071935714 20.91596 KIAA1551 1.53336279 5.04E−11 1.27E−08 136.1961962 372.187995 PLEKHG3 −3.801670968 5.17E−11 1.28E−08 16.75150993 1.15220756 ITGB2 −1.178163171 6.65E−11 1.61E−08 680.3715238 321.599435 ADAM19 2.136052341 7.96E−11 1.89E−08 21.82406658 82.6178222 MIR6723 1.364171561 1.27E−10 2.89E−08 5411.630952 15427.5075 FGR −3.427375695 1.25E−10 2.89E−08 203.5495476 27.5633367 FAM169A −3.933059942 3.05E−10 6.67E−08 9.554643095 1.556730675 FMNL3 1.886848299 3.25E−10 6.99E−08 4.030502919 14.83709891 MYO7A 4.503614597 4.04E−10 8.53E−08 0.038636476 13.82020979 LOC643406 1.080015874 4.92E−10 1.02E−07 8.94110381 21.4070425 LAIR2 −3.648996225 7.50E−10 1.53E−07 80.42070281 22.79628975 LINC00536 −2.30294596 1.18E−09 2.36E−07 4.489862762 1.01907325 AMICA1 1.778550701 1.75E−09 3.45E−07 119.9607238 344.4711 VIM 1.494086613 1.96E−09 3.80E−07 491.6159762 1106.35245 PRKDC 1.514072515 2.07E−09 3.93E−07 25.9920519 71.954705 PGK1 1.086986991 2.84E−09 5.22E−07 568.5674429 1142.7099 GNLY −2.081880512 2.82E−09 5.22E−07 3093.530095 635.69869 VPS13C 1.567869275 3.37E−09 6.11E−07 8.968918914 25.817943 CLNK 4.288010598 5.51E−09 9.83E−07 0.025489571 37.81930265 IL12RB2 2.207617883 6.35E−09 1.12E−06 5.973771714 19.9212583 MTRNR2L10 1.288450447 6.52E−09 1.13E−06 92.07810952 274.15068 C5orf28 −2.365273529 6.65E−09 1.13E−06 28.15868324 10.8994104 KLRF1 −3.513812967 7.27E−09 1.22E−06 193.5823511 14.4933614 MTRNR2L6 1.362905237 8.10E−09 1.34E−06 9.190159048 29.3221775 TMEM200A 3.469977659 8.52E−09 1.39E−06 3.816969743 49.1354955 MTRNR2L1 1.212300682 9.07E−09 1.46E−06 226.1222238 634.7212 SH3BP5 −2.89346707 9.37E−09 1.49E−06 13.15606714 2.10172946 GSG2 3.128523686 1.02E−08 1.61E−06 0.161439895 8.00016295 ANKRD28 2.439836494 1.20E−08 1.83E−06 14.10839 73.65266634 DUSP2 −1.186515607 1.20E−08 1.83E−06 1253.61149 578.04335 MTRNR2L9 1.337497628 1.39E−08 2.10E−06 319.5508095 1052.6489 CADM1 −3.798923884 1.73E−08 2.58E−06 14.14056571 0.541758555 USP28 −2.800231732 1.87E−08 2.74E−06 46.590065 8.1924564 CXCR6 2.074928075 1.95E−08 2.83E−06 215.3580623 799.8171578 OXNAD1 1.165357916 2.04E−08 2.92E−06 51.84040952 126.460545 LOC100129434 1.186595285 2.20E−08 3.08E−06 20.46000667 45.882185 ICAM2 −2.222167739 2.20E−08 3.08E−06 76.36366667 18.5619424 PGLYRP2 3.318268163 2.67E−08 3.69E−06 1.056929062 7.5602649 DNAJB1 −1.413412514 2.70E−08 3.69E−06 802.5950476 348.781017 EZH2 2.541688868 2.83E−08 3.82E−06 7.69050929 28.55026275 CXCR2 −2.872882644 3.15E−08 4.19E−06 25.0598669 3.1510207 CMC1 −2.098618623 3.54E−08 4.67E−06 334.9550048 76.791481 KIR3DL2 −3.16784706 4.66E−08 6.07E−06 44.4269131 5.5029878 PELO 2.512227676 4.82E−08 6.19E−06 14.90672521 80.21213 RIPK2 −2.592150871 4.85E−08 6.19E−06 51.12927 12.00812765 LITAF −1.186041133 5.17E−08 6.53E−06 489.3827143 224.55413 SRSF2 1.073461158 5.71E−08 7.05E−06 280.4715619 643.1785 ITGAM −3.288179636 5.69E−08 7.05E−06 20.78114599 9.55108064 SPON2 −3.08944003 5.92E−08 7.23E−06 68.93419057 8.0418225 EOMES −2.350328769 5.99E−08 7.24E−06 96.09277629 25.55344615 ELOVL5 1.194140558 6.30E−08 7.53E−06 77.90133333 188.919585 ABHD11 −2.246196046 8.68E−08 1.03E−05 28.72630952 8.1834175 RAB12 −1.056317965 8.94E−08 1.05E−05 315.4402381 159.223475 SH2D1B −3.653555023 9.20E−08 1.07E−05 22.65793566 1.67038849 EGR1 1.328069038 1.11E−07 1.25E−05 90.65356333 180.64925 RCBTB2 −2.761362657 1.26E−07 1.40E−05 65.87080476 19.41631984 ATP8B4 3.615872085 1.31E−07 1.43E−05 0.344809071 10.5492769 RGS1 1.374030196 1.31E−07 1.43E−05 756.459619 1916.0517 ASCL2 −3.713952755 1.33E−07 1.44E−05 10.87647238 0.357964 GPR25 3.450094942 1.71E−07 1.84E−05 3.260915429 49.20251905 SMURF2 1.900071442 2.11E−07 2.22E−05 13.87433392 39.638273 LOC101927374 1.11281412 2.13E−07 2.22E−05 12.26230476 27.5276295 KIAA0825 1.793395112 2.26E−07 2.34E−05 9.062423619 28.9025909 LARP1 1.334004962 2.41E−07 2.45E−05 9.35280619 28.1623345 SNAP23 −1.228426954 2.47E−07 2.49E−05 137.3034905 72.05703075 CISH 1.627753438 2.82E−07 2.79E−05 80.86795833 285.2522457 ZNF559 −3.025142135 2.84E−07 2.79E−05 25.17488419 6.09491092 SLC35D2 2.110097143 2.94E−07 2.84E−05 5.033894524 21.18080975 FAM65B −1.600964159 2.94E−07 2.84E−05 97.72626048 42.184457 LOC100130093 2.384781043 3.43E−07 3.23E−05 4.471636 23.500032 FAM129A 1.202394244 3.47E−07 3.24E−05 33.137718 72.1486845 ITM2C 1.911897049 3.58E−07 3.32E−05 67.57806086 250.0731207 ARHGAP25 −1.817060321 3.65E−07 3.36E−05 152.4628095 69.0699945 LOC100130872 −1.991189374 3.75E−07 3.43E−05 16.75408481 6.593254745 ZFAS1 1.199358326 3.85E−07 3.49E−05 83.68128333 177.09171 CYBB −1.793527414 3.93E−07 3.53E−05 64.10834148 11.9938415 PZP −2.888358053 4.16E−07 3.71E−05 8.12034131 3.009308975 HIST1H2BK 2.05603388 4.22E−07 3.73E−05 39.28850138 123.0758285 MALT1 2.349361739 4.71E−07 4.11E−05 12.3671099 42.27466225 RAPGEF6 1.241140351 4.89E−07 4.20E−05 28.62101667 67.59247 PRR5L −2.720570835 4.88E−07 4.20E−05 28.4360878 8.383760905 TM9SF3 1.719606775 6.14E−07 5.24E−05 22.233558 74.4089565 LINC00612 −2.822238193 6.87E−07 5.82E−05 13.02071243 2.1673256 RASGRP2 −1.960339855 8.11E−07 6.82E−05 104.0838905 30.4704815 MCF2L2 1.77037345 8.17E−07 6.82E−05 1.075314152 3.41337215 ADD3 −1.398797561 8.31E−07 6.89E−05 159.9318571 75.43510185 XCL1 2.033544975 9.53E−07 7.79E−05 30.51550386 141.3891085 MIR155HG 2.718579249 1.20E−06 9.74E−05 8.770128333 68.19258875 TTC39B −1.95836329 1.21E−06 9.74E−05 28.75757238 6.09487136 ZNF44 −2.534590645 1.29E−06 0.00010334 39.33668429 12.8156669 HNRNPD 1.22436894 1.34E−06 0.00010555 42.86327286 106.433995 TTC38 −1.605359191 1.34E−06 0.00010555 80.5307819 28.116408 FCMR −1.777542843 1.36E−06 0.00010696 200.2598944 80.13699245 LINC00504 1.081582043 1.55E−06 0.00012107 26.64122286 62.159235 SHOX 1.380820768 1.67E−06 0.00012931 0.463633286 1.33043075 PDE4D 1.630857417 1.69E−06 0.00012979 10.41079262 30.78342656 MORC2-AS1 −1.720968756 1.89E−06 0.00014451 63.34037095 20.5457955 CCR7 −2.464404119 1.95E−06 0.00014814 239.9860878 49.3558936 DOK2 −1.470162395 2.02E−06 0.0001519 324.6862381 127.6544189 ORMDL2 1.069442343 2.06E−06 0.00015351 113.456781 226.26465 RIN2 −2.966123994 2.09E−06 0.00015443 9.440358186 0.199756825 LDHA 1.049322824 2.11E−06 0.00015527 570.599381 1162.148 SFMBT2 1.948897349 2.13E−06 0.00015533 2.63146119 9.8511154 UGDH-AS1 1.248838088 2.28E−06 0.00016539 19.08877381 48.18311 IVNS1ABP 1.275070942 2.36E−06 0.00017027 148.0731952 400.824054 RASA3 −1.915627975 2.40E−06 0.00017163 66.32533333 21.9665584 MPI −2.353326054 2.43E−06 0.00017318 28.3699084 6.88767175 SIRT2 −1.499552785 2.62E−06 0.00018564 115.2741905 55.65131365 LILRB1 −2.073507487 2.81E−06 0.00019727 22.42856014 6.9053577 CDK6 1.800012015 2.84E−06 0.00019876 17.0048809 62.511125 CEP350 1.716329718 2.89E−06 0.00020049 10.43966743 38.48460117 CXCR3 1.429112179 3.21E−06 0.00022162 82.86109976 221.8926819 GPM6A 1.873719438 3.39E−06 0.00023287 0.336675162 2.202836 SWSAP1 −1.54571358 3.51E−06 0.00023827 14.06452133 4.7382231 NUPR1 −2.102624894 3.61E−06 0.00024356 110.0524586 23.0499858 SKIL 1.356725934 3.82E−06 0.00025447 36.2539719 73.784065 NUAK2 −1.977103288 3.84E−06 0.00025447 13.48667505 3.09541505 TANC2 3.089882387 4.04E−06 0.00026327 0.553212024 5.12779167 KLF7 −3.106136118 4.04E−06 0.00026327 6.978648795 0.610988815 CPNE7 2.987748945 4.14E−06 0.00026804 3.079236524 27.6457933 PCNX 1.679911819 4.23E−06 0.00027251 10.39656719 32.2845755 ACTN1 −2.74953546 4.27E−06 0.00027343 42.51342762 8.75996527 CUTC −1.221976687 4.67E−06 0.00029475 129.3552048 69.6624427 GIMAP4 −1.414835705 4.65E−06 0.00029475 445.6157337 190.8786594 PHF14 1.553582494 4.75E−06 0.00029802 5.411563848 12.4822209 LPAR6 −1.548072876 4.78E−06 0.00029823 71.46590388 27.4985452 COTL1 1.219750696 5.12E−06 0.00031774 444.2532048 838.82325 LDLRAP1 −1.734939687 5.18E−06 0.00031968 81.61549524 28.7617736 RNF44 1.68910313 5.26E−06 0.00032294 16.61935648 59.77938 OTUD5 1.846177309 5.54E−06 0.00033832 19.55586529 71.178468 FAM49A −2.526705269 5.63E−06 0.00034185 29.38951762 6.759340495 KIF5C 2.442095274 5.76E−06 0.00034817 1.484135657 9.82658695 PCBP1 1.474990375 6.32E−06 0.00037993 449.108119 964.3716 B4GALT5 1.766414298 6.54E−06 0.00039088 3.2958552 7.8889765 PERP 1.746555154 6.89E−06 0.00040982 20.26485792 93.237233 ILF3-AS1 −1.401809132 6.97E−06 0.00041245 48.10910714 23.90241965 CCDC144B 1.520739947 7.58E−06 0.0004459 4.185302905 12.8182025 RAC1 1.105059877 7.61E−06 0.0004459 139.2755238 233.57116 RPS6KA3 1.595115819 8.30E−06 0.0004838 12.37155557 34.417014 GIMAP7 −1.4037857 8.41E−06 0.00048791 764.0689048 367.0225432 DUSP4 2.262161797 8.48E−06 0.00048941 12.86056076 46.99192715 RPL5 1.176379227 9.00E−06 0.00051411 1708.037571 3358.533 DEGS1 1.103804675 9.31E−06 0.00052667 146.0186857 277.532665 LRP1 2.494030823 9.43E−06 0.00053103 1.215511952 4.593950775 FRMD4B 1.967481113 9.81E−06 0.00054713 17.27852171 39.86186793 DDX60 1.740019415 1.02E−05 0.00056735 14.20610748 59.79492642 ARHGEF1 −1.291779535 1.05E−05 0.00058041 212.2836 109.3370274 TMPRSS3 2.104017045 1.09E−05 0.0005971 0.990603448 7.09934565 ARHGAP35 2.020567437 1.13E−05 0.00061628 7.980023814 32.97274923 SPATS2L 1.568699983 1.15E−05 0.00061923 7.175204857 18.4375802 ZNF100 1.727488104 1.16E−05 0.00062335 1.838728252 14.04667465 CTSO −2.394862763 1.18E−05 0.00063276 87.34779524 28.05486479 SRGAP3 3.083987351 1.21E−05 0.00064522 0.327200095 6.54159999 CHN1 3.016328106 1.29E−05 0.00068228 3.889638771 26.56366172 ASB6 −1.409994218 1.29E−05 0.00068228 24.44135762 10.9425055 NT5E −2.612624338 1.49E−05 0.00077849 14.35977676 3.21892007 PUS3 −3.022511352 1.49E−05 0.00077849 14.78100877 0.55856775 CHMP6 −1.65237349 1.58E−05 0.0008245 90.8889381 32.4718011 LOC102723824 2.220309075 1.61E−05 0.00083527 8.376731619 17.913781 SRSF8 −1.272937322 1.64E−05 0.00084665 71.34348429 57.41924175 BIN2 −1.259196899 1.67E−05 0.00085116 491.3041429 219.0422681 ZFYVE28 2.300538964 1.70E−05 0.00086536 4.428132524 19.4064535 EHBP1L1 1.996718023 1.73E−05 0.00087228 7.180443862 25.8848635 PTRH1 −2.291611353 1.72E−05 0.00087228 71.97044286 18.2559359 ASCC3 1.525897011 1.80E−05 0.00090279 8.081438562 24.3278745 VDAC1 1.019841564 1.81E−05 0.00090279 141.0238476 235.935545 ZNF18 −2.631248872 1.81E−05 0.00090279 28.62923619 7.869980955 KIF21B 2.166012602 1.83E−05 0.00090745 4.438138 20.382474 LAG3 1.22701912 1.88E−05 0.00092214 87.38349743 217.4806655 ARHGAP11A 1.444394843 1.89E−05 0.00092638 2.611797762 6.46693175 PARP3 −2.89694382 2.07E−05 0.00100857 19.20667543 2.15505298 C16orf89 −2.402216829 2.23E−05 0.00107835 16.13956348 0.8268643 MTPAP −1.257160683 2.24E−05 0.00107991 28.74861643 13.57233135 TSPAN32 −1.711545059 2.28E−05 0.0010934 99.66786667 46.714372 RASSF4 −2.712424641 2.36E−05 0.00112716 38.27995619 7.67994325 HUWE1 1.478732178 2.38E−05 0.00113113 8.3189656 27.50419295 DOCK5 2.625035961 2.45E−05 0.00115108 1.4117016 6.88680025 TNF 1.537622653 2.43E−05 0.00115108 8.510453143 19.906433 ATP2B1 1.232541924 2.45E−05 0.00115108 53.28257048 120.203785 LZTS1 1.89060308 2.48E−05 0.00116116 1.530348495 6.11112885 CCL28 1.515771195 2.53E−05 0.00117224 6.587727714 13.8866925 POLH 1.362294764 2.52E−05 0.00117224 4.764934857 10.2461875 PLXDC1 1.910398886 2.56E−05 0.00117916 7.218845452 22.21014939 SLC38A2 1.119051382 2.56E−05 0.00117916 53.13309048 117.44348 ADCY3 2.560946699 2.64E−05 0.00120959 0.627494476 5.456133665 MTX3 1.669116918 2.69E−05 0.0012236 3.042661571 14.24616695 ZNRF1 2.828733845 3.09E−05 0.00139642 0.810231405 6.82966755 AKR1C3 −2.587366759 3.30E−05 0.00147422 35.08770348 11.6727318 KIR3DL1 −2.774117252 3.30E−05 0.00147422 39.17617897 4.93104205 KMT2D 2.0488088 3.44E−05 0.00152285 1.4962252 7.7576795 IFNG 1.783908689 3.48E−05 0.00153289 82.55949667 362.1978226 UBE2F −1.718368644 3.49E−05 0.00153289 55.71082381 21.82958125 BLOC1S3 −1.737490695 3.69E−05 0.0016159 37.0366489 12.4104362 ZNF350 −2.20269071 3.77E−05 0.00164559 39.55514852 17.20023995 KIR2DL3 −2.735915385 3.88E−05 0.0016848 29.8276564 2.69000165 CD14 −2.112206031 3.91E−05 0.00169426 74.05469481 16.6984343 SLC1A5 1.534783918 3.98E−05 0.0017162 33.13000886 96.9581249 RBMS1 1.704885397 4.26E−05 0.00180177 42.58026438 148.25084 NKTR 1.080979938 4.25E−05 0.00180177 34.011989 82.13539 PEX16 −1.01884714 4.27E−05 0.00180177 61.71854743 36.40784535 RAP2B −1.273692809 4.25E−05 0.00180177 80.36729524 34.5275585 TCOF1 −1.940096541 4.24E−05 0.00180177 20.13614476 8.973999545 HIVEP3 −2.001507989 4.39E−05 0.00184472 7.941141333 3.032539515 KEAP1 −1.22615691 4.45E−05 0.00186341 64.71328551 31.5632675 ARRDC3 −1.17054195 4.54E−05 0.00189564 211.8614238 112.5465397 MXRA7 1.658912627 4.91E−05 0.00204173 3.266608619 15.1301335 ESYT2 1.514083794 5.16E−05 0.00212136 12.05781967 47.540445 NMUR1 −1.773761089 5.15E−05 0.00212136 16.83415457 5.56423905 LINC00892 2.424521999 5.18E−05 0.0021239 24.90726819 170.14904 EIF2B3 −1.937296892 5.22E−05 0.00213377 62.28575714 26.8829665 UBA7 −1.274777668 5.25E−05 0.00213647 89.77450238 52.6846935 OGFRL1 2.379697737 5.32E−05 0.00215872 3.896613762 30.0878519 NOSIP −1.206988658 5.58E−05 0.00224595 172.3891771 90.073862 APOL1 −1.660848056 5.69E−05 0.00228344 14.78162748 5.5118258 NKX3-1 −2.804697893 5.79E−05 0.00231464 5.651472695 0.701179405 ZNF441 −2.454868826 5.97E−05 0.00236312 8.393922424 2.879041465 YBX3 1.716666696 6.04E−05 0.00238334 24.78591295 61.299884 PARD6G 1.312295036 6.07E−05 0.00238688 4.132179238 12.0712125 LYSMD1 −2.924027783 6.10E−05 0.00239096 6.796088933 0.4503092 KPNA4 1.45711023 6.13E−05 0.00239269 11.01992676 29.1450691 TMEM212 1.058248599 6.45E−05 0.00250388 13.60060286 32.280461 CTLA4 1.435048574 6.53E−05 0.00252477 19.54678733 68.7486734 MYADM 1.060424765 6.59E−05 0.00253937 53.35683857 123.7034305 KLHL5 1.772588118 6.64E−05 0.00254182 2.376725143 9.9981583 PIP4K2A −1.288193495 6.63E−05 0.00254182 967.4229524 424.5596301 KLHL6 1.598790907 6.70E−05 0.00255588 5.091936467 12.3455271 C15orf53 2.610180897 6.95E−05 0.00262468 2.301744476 24.90123835 DAPK2 1.912434282 6.92E−05 0.00262468 9.456853214 36.7584622 TPST2 −1.273052932 7.03E−05 0.00264731 263.7426048 125.121244 TMEM41A −1.720382667 7.10E−05 0.00265702 28.74279305 15.40182055 PLAUR −1.417464169 7.38E−05 0.002748 271.6738353 50.50656925 RBPJ 1.574107492 7.44E−05 0.00275597 65.66283667 211.5952325 LRRC37A3 2.074130192 7.55E−05 0.00279009 0.480966952 2.98394201 EPAS1 2.318444592 7.60E−05 0.00279574 6.038517781 26.05039855 YIPF6 −1.816114456 7.61E−05 0.00279574 12.21552449 5.799607305 TMED1 −1.817944234 7.80E−05 0.00285387 44.66887619 12.76184885 NCOA7 1.293829014 7.85E−05 0.00286513 11.85079605 33.3728845 TRNT1 −1.56957745 8.10E−05 0.00294561 41.74942476 21.60956825 PDCD1 1.960356621 8.23E−05 0.00298567 41.06805581 134.177045 KRT86 2.801705688 8.31E−05 0.00300334 0.77714719 21.05319575 CAPG 1.160396095 8.39E−05 0.0030223 604.9114381 570.63815 CYP51A1 1.217984916 8.92E−05 0.00318443 52.71879524 115.478695 SQLE 1.651331611 9.30E−05 0.00330959 23.50773952 69.026012 EPB41 −1.085276175 9.69E−05 0.00343927 35.59453905 24.4307317 SNRNP35 −1.380326304 9.96E−05 0.00352319 32.07545762 15.3377564 PLEKHO1 2.101609708 0.00010076 0.00355319 10.75456533 34.77226 TFDP2 −1.074732513 0.00010209 0.00358918 13.8775519 7.722759 SLC34A2 −2.614315405 0.00010472 0.00365947 12.70014439 1.766653745 MYC −1.570814137 0.00010637 0.00370606 58.92450929 22.44566625 TMED5 1.08124454 0.00010738 0.00372154 29.16007667 50.03997411 NTPCR −2.395567938 0.00010746 0.00372154 64.32159367 21.7462204 WNK1 1.318502857 0.00010895 0.00376211 9.170893619 27.9849355 XIST 1.767227681 0.00010955 0.00377155 1.196562429 6.024847915 SPC25 1.170654762 0.00011181 0.00383787 9.154391429 23.645568 DENR −1.22922827 0.00011379 0.00389424 73.8563619 47.0460279 KIF11 1.821662005 0.00011551 0.00390267 2.443237967 9.257986 GOLIM4 1.777868817 0.00011534 0.00390267 30.93483438 62.605315 EPHA1-AS1 1.589736701 0.00011571 0.00390267 2.328378471 8.8394592 SORL1 −1.124650308 0.00011568 0.00390267 32.53818476 15.6531715 INIP −1.222448299 0.00011929 0.00401168 91.57427238 52.308057 FAM228B −2.041900144 0.00011983 0.00401813 13.19441343 5.38700615 UAP1L1 1.889609098 0.00012291 0.00410963 2.936810938 12.80484475 SMG9 −2.185540007 0.00012737 0.00423412 25.12799343 8.71863 TUBA1C 1.655052435 0.0001324 0.0043889 25.54587348 56.705964 LINC00341 1.789049608 0.00013297 0.00439536 12.578937 45.24209 CBFB 1.571033412 0.00013589 0.00445864 68.46266429 222.2403375 POLR2E −1.2088594 0.00013715 0.00448244 101.8120714 47.35464315 ATL2 1.645098431 0.00013893 0.00452778 6.926690762 19.8609125 CD27 −1.282713519 0.00014204 0.00461599 149.9836976 69.1602188 KCNC4 2.525809224 0.00014404 0.00462919 2.09133519 9.068993435 KLF13 1.405276951 0.00014394 0.00462919 3.886962667 12.13907515 GUSB −1.087842847 0.00014291 0.00462919 127.3000857 59.46871275 LPCAT1 −1.887466956 0.00014327 0.00462919 50.49580452 12.65491646 TBC1D25 −1.991487663 0.00014464 0.00463568 22.69922333 7.652385165 EFNA5 −1.780188118 0.00014796 0.00472898 8.80737419 2.9897214 LDB1 2.211714353 0.0001558 0.0049523 12.27335562 46.658046 GSN −1.856153002 0.00015695 0.00497514 155.9601187 24.92560408 AGTPBP1 −1.190748054 0.00015767 0.0049843 28.04230333 17.07175222 CST7 −1.279211634 0.0001628 0.00510307 1822.877929 814.3378435 GZMK −1.465391434 0.00016271 0.00510307 1090.510599 482.9047815 RAB3D −1.646487305 0.00016243 0.00510307 12.54113167 5.76880282 FAM65A −2.15466771 0.00016318 0.00510307 12.80896311 2.763608925 DENND6A −1.156167299 0.00016556 0.00516338 20.3185 12.27502635 THOC3 −1.44634318 0.00016754 0.00519729 9.395982857 5.46055835 SLC7A5P2 1.461890782 0.00016864 0.00521747 14.76301557 41.072381 GNB1 1.595767092 0.00017132 0.00528383 23.07147667 81.62131885 CCDC65 −2.34098191 0.00017364 0.00532954 29.07540152 6.60117545 ZNF786 −1.977944939 0.00017677 0.00541118 14.74838324 4.00298737 TRIM24 2.423827168 0.00018028 0.00547555 3.644654986 26.3156509 CARS2 1.693420689 0.00018023 0.00547555 20.01825719 53.228753 CD226 2.11664127 0.00018211 0.0055145 20.47994643 94.98832335 PLEKHJ1 −1.42449896 0.00018252 0.0055145 111.3896667 45.58957835 ADRB2 −1.990923947 0.00018579 0.00559893 133.6191638 49.61041635 MINA −1.917614858 0.00018639 0.00560232 10.84250714 3.72207806 IL11RA −2.083504821 0.00018875 0.0056502 22.64916552 7.2149125 DPEP2 −2.205657439 0.00019126 0.00570442 43.10033762 17.71765105 LOC440434 1.839111218 0.00019544 0.00581394 2.232405714 8.04353135 C1RL-AS1 1.925490425 0.00020441 0.00604984 0.764437424 2.0629908 SLAMF1 1.97751179 0.00020791 0.00613785 34.87498443 131.8489772 PDE4A 1.514702226 0.00021021 0.0061774 8.788954519 22.24409349 TRAF3IP3 −1.023757033 0.00021031 0.0061774 175.9603465 110.8125055 SEL1L3 1.447604044 0.00022308 0.00651921 34.67522048 105.587455 DOCK10 1.025366504 0.00022731 0.00662638 22.75661862 55.525593 AGO2 1.913492388 0.0002291 0.00665622 6.69428181 29.835073 APOOL −1.4599624 0.00022994 0.00665622 9.866991905 4.9035758 SLC4A1AP −1.500967401 0.00023006 0.00665622 60.90765484 30.57019365 GLYR1 −1.493452199 0.00023127 0.00665804 50.69578571 23.97702565 TESK1 2.335172957 0.00023342 0.00667018 3.665402619 25.04709 SLC7A5 1.396734709 0.00023283 0.00667018 26.18238476 61.6948623 HMHA1 −1.095094418 0.00023328 0.00667018 153.4711905 82.52380625 NR2C2 1.98376907 0.00023918 0.00681814 2.334334629 8.99618895 AP4B1-AS1 1.303155865 0.00024286 0.00690603 3.367507905 10.55503925 UNKL −1.80143226 0.00024644 0.00697362 3.032874143 1.82100178 ING3 −1.406744774 0.00024721 0.00697847 56.85451143 28.07718918 ANKRD32 1.443730347 0.00025762 0.00723698 33.46615714 104.99121 NPIPB5 1.526741654 0.00026067 0.00728736 2.765647476 6.606986 DOK6 2.513396625 0.00026462 0.00737965 0.146552833 2.537527625 SNX27 −1.40073971 0.00026524 0.00737965 37.33825619 15.8946053 BBS2 −1.950926604 0.00026753 0.0074255 74.32054286 26.4182932 RASA1 1.354757826 0.00027325 0.00756612 13.71942938 31.58191005 SLC39A11 −1.598648182 0.00029385 0.00809794 35.99148971 14.80168625 PITPNA 2.312434612 0.0002998 0.0082228 3.488508 21.7398403 RAD52 −1.50567048 0.00030556 0.00836103 9.932621324 4.26503812 HRSP12 −2.383100619 0.00032349 0.00881022 27.23581476 9.8728184 SMG1P3 1.200042025 0.00032556 0.00881529 1.363507952 2.71159545 RRP7A −1.165768141 0.00032596 0.00881529 39.24837762 20.3423858 POLR3H −1.67593178 0.00032576 0.00881529 13.8710933 6.146357295 DHFRL1 −2.359797868 0.00032712 0.00882622 5.099411576 0.47705205 TYMP 1.176092072 0.00033212 0.00894032 87.24913762 104.307335 LPCAT3 −1.27735195 0.00034009 0.0091335 79.39185714 48.14817045 C5AR1 −1.851391779 0.00034576 0.00926439 27.82386229 11.66871945 ATF3 2.056842456 0.00034687 0.00927285 10.44020133 37.6365183 CEP97 1.50396083 0.00035088 0.0093585 3.274631229 9.616456 KIAA0513 1.368258293 0.00035513 0.00940686 3.681573657 5.3122714 KIAA0020 −1.159965895 0.00035504 0.00940686 73.4876049 41.18979315 CCR6 1.511032115 0.00035676 0.0094153 14.34848795 23.36445165 CPSF2 1.241995889 0.00035947 0.0094423 10.72023305 27.40330205 METTL21A 1.094244219 0.00035972 0.0094423 14.63200143 35.4652285 NUPL2 −1.832195681 0.00036147 0.00946668 51.23907567 20.4746829 ASTN2 1.069590281 0.00036295 0.0094842 5.929357857 12.6587515 TBCK −1.802592091 0.00036698 0.00956796 10.19409643 3.7038319 ARFGAP2 −1.05571895 0.00036939 0.00960914 99.27014286 55.69149165 VMA21 1.296350724 0.00038667 0.00999153 14.90407952 47.51696 BEX2 −1.953762446 0.0003889 0.01002659 42.92123476 16.633287 C20orf194 1.79920571 0.0003994 0.01027462 3.980905681 14.94492252 RAB29 −1.369925477 0.00040951 0.01048821 88.32204762 50.2578652 NASP 1.310206027 0.00041112 0.01050624 30.34420248 71.02776 C1QTNF6 1.673867441 0.00041528 0.01058907 5.089702905 18.4102024 PDE7A 1.250028815 0.00041853 0.01062531 13.26747514 34.9504633 SLC31A2 −1.775058927 0.00042405 0.01071861 216.1743924 43.5336665 KLHL2 2.248561156 0.00042573 0.01073759 4.456770238 16.28283281 FERMT3 −1.204568177 0.00042945 0.01080807 223.1386952 105.6600795 WRNIP1 2.312410653 0.00043286 0.01082584 4.016170619 36.14411837 PPP1R15B 1.531191906 0.00043296 0.01082584 13.55361243 41.80690205 DENND2D −1.230933468 0.00043207 0.01082584 177.521881 107.0518394 PDS5A 1.494586311 0.00044007 0.01097996 14.22353581 43.45307149 SIGLEC9 −2.390836951 0.00044441 0.01106438 15.99866633 4.49902325 LOC101927482 2.304003046 0.00045298 0.0111772 8.79705 70.4761062 MBOAT2 1.345839205 0.00045237 0.0111772 2.96217119 9.6344485 ATF6B −1.566818376 0.00045322 0.0111772 17.59367905 7.97181365 PATL2 −1.642281469 0.00045186 0.0111772 104.1426341 31.97329445 GRN −1.063273506 0.00045971 0.01129978 791.2872381 264.725843 ERI1 −1.242477967 0.00046365 0.01134862 19.76817686 16.5382545 NCALD −1.68205333 0.0004633 0.01134862 33.27944905 17.4302691 FCGR2B −2.254062836 0.00046724 0.0114124 12.38491424 1.09008495 KIR2DS4 −2.340829735 0.00047309 0.01153119 26.8382171 3.872648 HAUS2 1.244208464 0.00048082 0.01169486 16.20686571 35.1828865 RALGAPB 1.085068861 0.00048941 0.01187895 8.581074286 19.9329055 MAP4 1.567107719 0.00049617 0.01201785 23.17886282 75.32267281 NPIPB4 1.612741663 0.00050141 0.01206927 1.314467681 3.3028621 ZBTB9 −2.327534581 0.00049936 0.01206927 9.2692554 4.62615606 CASS4 1.232728611 0.00050479 0.0121004 10.33679177 23.91917485 MED19 −1.434707891 0.00050469 0.0121004 44.44179571 28.0865765 IRF4 1.655714921 0.00050764 0.01212763 8.881926324 37.019321 MYO1F −1.066903485 0.00051053 0.01216259 124.5430429 64.6513293 RRN3P2 −1.714465278 0.00051396 0.01221926 9.252999857 5.3255443 FAM134B 1.638213865 0.00052287 0.01238031 15.5642671 40.45138445 POLR2C −1.501187058 0.00052867 0.01249202 101.9659667 54.6541517 CYTH1 −1.0767005 0.0005339 0.01258999 220.3660476 123.9829496 NFYC-AS1 −1.747707738 0.00053774 0.01265488 7.052072429 4.10523765 FAM101B 1.746294114 0.00054483 0.01279574 11.1097819 46.8396593 NFIA −1.895142906 0.00054804 0.01282844 6.960684619 3.316372105 DTX3 −1.965784578 0.00054843 0.01282844 11.36799743 4.4052707 NCBP2-AS2 −1.27324026 0.0005636 0.01310398 125.8263524 62.2609764 C2orf74 −1.605791569 0.0005628 0.01310398 47.69721333 15.21258005 EXOC8 1.393473812 0.00057299 0.01323641 5.879713952 14.19818165 HIPK1 1.259329438 0.00057386 0.01323641 10.37371019 29.54598748 PTRHD1 −1.524952775 0.00059245 0.01361117 112.4339619 51.295813 SATB1 −1.450814725 0.00059441 0.01362909 29.0269319 14.87791935 PIGW −1.70243522 0.00059856 0.01367036 22.70485962 9.56260825 ARMT1 −2.138001294 0.00059855 0.01367036 30.45736219 12.96236292 CD96 1.082135365 0.00060674 0.01382991 190.6091374 445.927872 FAM102A 1.645022975 0.00061089 0.01384277 15.8821847 44.713496 TESPA1 −1.224535174 0.0006107 0.01384277 34.09646505 15.9437695 HHLA3 −1.783287692 0.00061015 0.01384277 15.31946967 19.470709 PHACTR2 −1.249051197 0.00061944 0.01400934 20.96662857 9.260568 UBE2Q2P1 1.068947583 0.0006375 0.0143339 8.930663857 20.2732585 MDFIC 1.551874807 0.00064418 0.014445 14.88952865 55.02251445 EAF1 −1.299453137 0.00064493 0.014445 43.09910952 24.90341265 FAM102B 2.109586468 0.00064845 0.0144927 2.63702719 27.78515825 CDIPT −1.217251575 0.00064956 0.0144927 91.82493667 48.5642925 PPP3R1 1.876147494 0.00065664 0.01462255 6.446798633 21.37249 SACS 2.076350394 0.00067552 0.01498534 2.101448033 12.0727165 TSC1 −1.54806369 0.00067936 0.0150419 12.64261554 5.67724477 PPME1 −1.827792346 0.00069454 0.01534862 39.13166571 21.647448 MAPKAPK2 1.96713612 0.00071533 0.01565905 0.816921471 4.38154145 PCMTD2 −1.425377237 0.00071735 0.01567368 54.46934619 27.86172965 LINC00116 −1.556841189 0.00072237 0.01575373 27.26614214 11.7182429 ACSL4 1.827216948 0.00072841 0.01585224 12.69324183 30.4276168 PI4KA 1.676670157 0.00073035 0.01585224 4.408701 18.24665 HOPX 1.210396178 0.00073396 0.01587589 204.3492705 494.9027762 LYAR −1.051479502 0.00073619 0.01587589 162.0467619 92.5870975 ELMO2 −1.107488358 0.00073552 0.01587589 106.4386476 49.46063745 DENND1A 1.80462059 0.0007407 0.01594357 6.1231801 23.68573804 CYB561 −1.489223981 0.0007513 0.01611206 17.6988591 8.5005074 TMIGD2 1.252962855 0.00076016 0.01623275 14.96517143 31.062332 NQO2 −1.352493557 0.00076113 0.01623275 60.58205 25.7614949 SKI 1.947359558 0.00077423 0.01645171 0.392191043 3.10283384 RAB21 1.304362685 0.00079042 0.01673444 23.18509605 58.62239915 CSF3R −1.944066722 0.00078996 0.01673444 23.72629945 5.661690165 PCNXL3 1.820209186 0.00079838 0.01687221 3.427240362 13.71444475 CARD8-AS1 −1.224524067 0.00080413 0.01696266 19.04523843 11.228074 MAT2A 1.005887405 0.0008242 0.01735462 89.89590476 154.450275 TADA2B 1.222867145 0.00083479 0.0175458 4.002363048 9.47839305 STX3 1.499915799 0.00084387 0.01767262 11.63656086 22.42890895 JMY 1.914684894 0.0008624 0.0178058 2.233548633 9.213031 BTBD7 1.723887579 0.00085229 0.0178058 3.343328867 11.70509428 RSBN1L −1.459929578 0.00085752 0.0178058 8.030019667 3.9945429 CIAPIN1 −1.565209613 0.00085533 0.0178058 77.74407619 41.0017459 DOK1 −1.768374315 0.00085583 0.0178058 37.27138333 13.1786472 DCP1B −2.073917448 0.00086078 0.0178058 37.24625476 12.0182602 MANBA −1.808919441 0.00089272 0.01836417 18.8245423 7.538196335 MTSS1 −1.761070691 0.0008946 0.01837015 18.5287793 6.52629872 RPS2 1.110924704 0.00090508 0.01855263 1951.231333 3025.4425 ITGA5 −1.332979911 0.00091178 0.01865696 62.5509 26.01677665 ARID3B 1.475041207 0.00091604 0.01867827 13.15811477 46.9491545 CD300A −1.832905746 0.00092368 0.01880092 113.4101663 39.52995785 POLR3F −2.271732688 0.00093671 0.01899954 17.56640771 5.199307 MXD4 1.476173375 0.00095074 0.01918342 9.359247619 27.3796455 CPNE8 −1.877032265 0.00097453 0.01962939 15.75364538 5.43907834 NCF4 1.357098972 0.00097951 0.01966147 56.53410076 86.755054 DUS3L −1.484396682 0.00097858 0.01966147 36.38678795 14.53410265 CDC26 −1.173174999 0.00100179 0.02007394 238.7674286 119.7321551 TGFBR3 −1.435187571 0.00100384 0.02008029 67.24751205 29.94398345 HDDC2 −1.268862316 0.00101088 0.02018626 123.8036667 67.9808733 SARDH 2.294096038 0.00102414 0.02034607 3.728724762 20.16373173 NMRAL1 −1.475564497 0.00102413 0.02034607 101.2016619 42.4074745 DDX49 −1.679761061 0.00102414 0.02034607 32.13191195 11.88200645 GOLGA8B 1.128453104 0.00103932 0.02061237 7.629956238 17.656008 APMAP −1.026634309 0.00106369 0.02105967 533.4089048 258.1887565 SPSB3 −1.105088531 0.00106718 0.02109281 217.7387857 123.0655227 ETV1 2.434846563 0.00107798 0.02123713 0.310453333 9.36143927 TNFSF14 1.050001711 0.00108212 0.02123713 22.60123595 60.4481919 RANBP3 −1.327147463 0.00108128 0.02123713 38.6904619 14.96831215 ATP10D 1.855742812 0.00109212 0.02133783 4.340767567 19.78619264 LOC102606465 −2.447368854 0.00109246 0.02133783 14.16471976 0.27695965 TTN 1.460996679 0.00110171 0.0214824 0.51753301 1.68269195 TRAF3IP2 1.097128158 0.00111682 0.02170402 4.3813 10.520044 HEXA −1.214689315 0.00112365 0.02176397 190.0591476 100.2384131 FRY −2.380982116 0.00112832 0.02180454 1.7706065 0.08596388 BID −1.335634005 0.00115289 0.02218219 80.79564286 33.02446055 AGAP2 1.25933984 0.00116449 0.02236824 12.47762385 25.56242397 SLAMF7 −1.097981708 0.00118377 0.02270106 154.3703411 97.756458 GPX3 −2.009859501 0.0011912 0.0228058 68.71822389 10.9501879 INPP5F 2.094388653 0.00119747 0.02285048 0.683434986 6.52550963 PTMS 1.527260067 0.00119677 0.02285048 14.55434505 39.8358905 ATF4 1.13820403 0.00126634 0.02400673 66.5021559 132.054105 RSU1 −1.034060739 0.00128444 0.02427042 113.3402333 76.4465641 ICAM1 1.307628761 0.00128715 0.02428207 45.27852671 106.998965 GNAI2 1.431878899 0.00129323 0.02431769 77.9547819 225.6534442 RFX7 1.493182438 0.00130679 0.02453303 9.145289048 19.7635245 AFAP1L2 2.217430641 0.00131729 0.02461261 0.917768462 5.888400985 STX11 −1.074261581 0.0013174 0.02461261 45.60824286 24.79838315 CEP78 −1.731280746 0.00131509 0.02461261 62.41426857 25.59219025 SERGEF −1.628390874 0.00132889 0.02474767 51.05715719 27.04479875 KLHDC3 −1.485905935 0.00135796 0.02516789 105.7995905 44.6288617 MCU −1.781678671 0.00135587 0.02516789 21.059248 7.2983622 ZNF43 1.365362673 0.00136523 0.02526219 5.320740857 19.68480875 STT3B 1.445687084 0.00137371 0.02528219 50.40380312 108.1294734 ZGPAT −1.2667066 0.00136929 0.02528219 63.04500952 31.116643 BINI −1.286241264 0.00137503 0.02528219 115.9730212 47.48353135 PRAF2 1.088429664 0.00139187 0.02547835 52.76120524 130.40493 FBXL15 −1.245548804 0.00139228 0.02547835 34.4780081 14.03420705 CMKLR1 −2.393657255 0.0013904 0.02547835 7.379469857 0.01679331 PROS1 −2.381361313 0.00140406 0.02561766 14.30501987 0.271954765 UBAP2L −1.107731449 0.00142117 0.02584397 60.0890381 32.03060748 ILF2 −1.322111404 0.00144866 0.02626157 268.6788571 134.0617756 SOS1 1.916563335 0.00146636 0.02649961 3.472780429 14.67357545 CLU 1.445639436 0.00147363 0.02658954 33.64088286 70.5663326 DENND1B 1.31053697 0.00148785 0.02680441 8.868308262 27.4410549 CCDC137 −1.424536409 0.00149865 0.02695706 45.84479614 26.23133495 COMMD1 −1.163651607 0.00151914 0.02728341 155.913578 80.0766678 ECT2 1.625708892 0.00153602 0.02754382 1.838423486 6.2113756 TNFRSF4 −1.818299294 0.00154166 0.02760233 21.45005543 5.2371664 ERBB2 −2.074275535 0.00154754 0.02766499 4.287361633 0.655102315 PTGDS −2.288741647 0.00155432 0.02770077 28.53224938 4.8148675 GZMM −1.082018373 0.00155738 0.02771274 283.5499905 139.63146 UBE2E2 −2.05240565 0.00158064 0.02804073 20.36653043 15.0806748 RMDN1 −1.415147467 0.0015921 0.02820087 36.57705833 15.18240745 CTDNEP1 1.714000117 0.00160704 0.02842206 16.60610719 63.6246042 CDC42SE1 −1.188507354 0.00161548 0.0285278 224.792919 115.0242707 IPP −1.320206248 0.00164409 0.02894493 18.17084054 11.66533725 CDKN2D −1.36879016 0.00166031 0.02909798 96.05093448 58.9614505 ATP1B1 1.283027758 0.00167718 0.02914164 52.0284119 75.36316685 UBR4 1.08473922 0.00167246 0.02914164 4.653459695 11.783862 KIR3DX1 −1.049750649 0.00166595 0.02914164 4.466350167 1.73610435 OXLD1 −1.420013153 0.00167581 0.02914164 60.42109286 34.3730691 WDR37 −1.670567502 0.00167227 0.02914164 23.3282819 9.586654835 PPOX −1.109942601 0.00169085 0.02932322 56.83536667 22.04271505 TGFB1 1.171557058 0.00169425 0.02933837 34.42022667 69.536372 ZNF557 −1.236244688 0.00170752 0.02952401 20.62738043 9.55651765 ULK2 1.325400012 0.00171981 0.02969224 1.888480762 4.9796416 CYP2U1 −2.049943251 0.00174364 0.03005893 11.44327268 5.70043324 NR3C1 1.031848789 0.00175779 0.0302579 38.35911238 94.7195966 SLC27A2 1.98883201 0.00181163 0.03090959 7.761453905 26.6987376 TFPT −1.502632807 0.00182073 0.03101928 30.40861905 12.54043605 MKI67 2.213790722 0.00182667 0.03107475 0.396551648 2.29143949 CST3 −1.160291651 0.00184011 0.03125756 137.9140714 67.30464325 LSR 1.60329948 0.00187247 0.0316448 21.59478005 55.67163075 DPP4 1.299281837 0.00187217 0.0316448 30.0033817 77.8169133 CASP1 −1.156469494 0.00187381 0.0316448 205.0463841 99.98735945 AHSA2 1.382667831 0.00187723 0.03165638 14.3165809 35.795771 TARS2 −1.214923276 0.00191337 0.0322191 45.92252857 26.3175739 ZDHHC11 2.168394746 0.00192446 0.0323588 2.041777762 22.7649697 ZNF10 1.777188919 0.00192914 0.03239061 3.195804 10.83783919 IGSF6 −1.844095235 0.00195275 0.03269235 106.8541425 21.85815275 FAM208A 1.049249105 0.00195718 0.03271935 14.16893262 34.97957965 MTMR9 1.315310954 0.00196674 0.03281718 5.720141533 16.17714945 CSRP2BP −1.82037327 0.00197683 0.0329056 16.6018126 8.3935547 PASK −1.686985134 0.00198289 0.03295917 14.93246762 7.15466456 MKKS −1.437049717 0.00200493 0.03327787 38.0899919 17.0888044 FAM46A −1.81198712 0.00202033 0.03348561 11.81714486 5.579773015 ITFG3 −1.702008908 0.00202437 0.03350458 20.83019762 11.13164403 DLG5 −1.953834863 0.00203104 0.03356717 3.435019381 0.903906625 KIAA0101 1.444676814 0.00204015 0.03365834 6.675146857 25.12704715 SAAL1 −1.583018596 0.00204236 0.03365834 51.56066124 17.6734762 MMP25 1.459299708 0.00206447 0.03383054 2.632463762 7.9999775 TATDN3 −1.074764039 0.0020589 0.03383054 28.73702095 12.906969 LOC100996447 −1.156694083 0.00206329 0.03383054 42.42375095 23.50972975 NIFK-AS1 −2.019056074 0.00205963 0.03383054 11.83977338 3.4095151 XPR1 1.392572867 0.00207024 0.03387718 3.614010729 9.8424576 C11orf21 −1.545286707 0.00208323 0.03404167 43.01835476 16.9673255 ICOS 1.37194015 0.00213471 0.03468756 52.45500238 122.4600494 TULP4 −1.370180377 0.00213448 0.03468756 11.6970605 6.69757389 INPP4A 1.32469001 0.00214188 0.03475543 11.57118199 28.38812305 DFNB31 1.739227223 0.00216261 0.03499379 3.190885052 11.18772015 ANKDD1A 1.666867831 0.00216252 0.03499379 2.474719238 7.0630617 PTPRJ 1.045068081 0.00216996 0.03506395 7.210654619 22.43940385 DST 1.781307402 0.00218105 0.03519407 0.740807522 2.481083109 SMIM3 1.701657926 0.00219219 0.03522757 22.12527529 101.0869653 MARK2 1.627042544 0.00219035 0.03522757 3.95061229 13.006327 LPAR5 −1.432358065 0.00219223 0.03522757 37.58498552 22.93229028 PRMT5 −1.713226626 0.00220091 0.03531809 23.97953357 9.213471305 DMKN −2.116833951 0.00220939 0.03540511 7.914099052 1.584939 PIGL 1.533178933 0.00222549 0.03561394 17.87966267 48.98544735 CXorf23 1.379998376 0.00224041 0.03580339 2.371735467 14.63350675 ERLIN1 −1.898071983 0.00225202 0.0358865 20.30472526 6.76962096 ZNF689 −2.141277674 0.00225575 0.0358865 9.676069048 2.30918672 UQCC3 −1.448663386 0.00227194 0.0360096 12.19188619 5.86697545 ZNF587B −1.482821582 0.00228038 0.03609416 14.89556113 7.10224025 ENPP5 −1.963798982 0.00229414 0.03621307 19.36512957 9.023781 MRC1 −1.322294944 0.00233316 0.03662963 52.31267884 15.67313203 HDAC6 −1.449599247 0.00233315 0.03662963 28.30981905 11.9235349 CEP63 −1.646464036 0.00233294 0.03662963 12.22384563 5.27964766 CREM 1.063360811 0.00234737 0.03670373 42.30688857 71.516919 SYNE1 −1.009975398 0.00234287 0.03670373 16.10141843 10.3669361 SVIL −1.361066127 0.00234573 0.03670373 9.580544286 4.22403719 NME4 −1.694305479 0.00235394 0.03675689 15.39367581 6.5277521 ZNF48 2.001817403 0.00235989 0.03680028 0.95379711 2.93424308 HIATL2 1.639605506 0.00237954 0.03691362 11.04763529 30.088932 TNFRSF10A 1.233874024 0.00237704 0.03691362 39.42923329 87.286662 PAG1 1.142151432 0.00237849 0.03691362 13.7221171 36.40958485 MELK 1.140922968 0.00237988 0.03691362 2.926656905 9.4487255 AGAP6 −1.615980385 0.0023937 0.03702892 4.65957 4.15214054 DDA1 −1.010469768 0.00241255 0.03727088 18.96847857 13.5441625 ARFRP1 −1.283380491 0.00243869 0.03762465 46.60115286 22.098322 RCOR1 1.944709033 0.00244246 0.03763275 0.534440586 4.51260244 PDK2 −1.664734422 0.00246036 0.03770812 29.69540175 11.07580269 FAM216A −1.983848294 0.0024698 0.03780297 13.79144952 6.5779684 VAMP5 −1.136383361 0.00247878 0.03789039 158.8593286 84.24594405 HERC1 1.091641582 0.00253247 0.03866021 9.368462695 26.80007538 ZNF555 −1.181944735 0.00253582 0.03866035 3.489925914 1.97726846 UBE2S 1.605973396 0.00254412 0.03873612 52.33753024 129.0238405 CCDC28B −2.032747097 0.00257249 0.03901441 13.39630476 2.8255758 TRA2B 1.071850101 0.00258234 0.03906167 34.72536619 75.910455 DERA −1.577728539 0.00257957 0.03906167 43.97195548 20.5084549 TSPYL2 −1.053848144 0.00260025 0.03923037 91.85230476 58.63886275 PKIG −2.077214187 0.002643 0.03977177 10.41545852 1.8342887 CCAT1 1.445071665 0.0026754 0.04006706 0.528602548 16.95488595 C1orf35 −1.378296383 0.0026703 0.04006706 39.94078238 24.80257585 CDKN1B 1.282793989 0.00272467 0.04068418 37.4884511 115.6148839 CISD3 −1.10729904 0.00274379 0.04091698 43.8215181 21.41737 CKS2 1.378226136 0.00275569 0.04092812 44.10446333 217.9010409 IL4R 1.173407546 0.00275832 0.04092812 20.8707369 44.223466 ANGPT2 1.090592385 0.0027657 0.04092812 1.214743286 4.68820725 SEC24C −1.065187919 0.00275712 0.04092812 78.9841681 45.24677965 VIPR1 −2.245159841 0.00276548 0.04092812 7.878956714 0.078206365 MED14 1.56112954 0.00281212 0.04150917 4.157886333 12.40530445 AHI1 1.317518043 0.00281763 0.04153763 8.087297457 23.78564948 DPH6 −2.003375054 0.00282831 0.04164227 9.295661143 3.259904245 EHD1 −1.001672515 0.00283359 0.04166709 59.14083405 36.4075224 PSPH −1.152155764 0.00284639 0.04180226 10.84557571 8.5721058 SH3GLB2 1.512043303 0.00285527 0.04187964 6.430948286 19.3427 ARHGAP26 1.202808995 0.00290391 0.04237881 4.392610524 14.80365015 HIF1A 1.052731671 0.00290373 0.04237881 38.03787619 90.85415895 MYO9B 1.008632072 0.00290113 0.04237881 8.362505238 25.050393 MUS81 −1.134364426 0.00289302 0.04237881 28.52751952 18.2275496 HKR1 −1.831633144 0.00292061 0.04250344 22.39177522 8.01066489 PTAR1 1.699146857 0.00294713 0.04268765 2.743866857 8.368398865 TMEM187 −1.825720333 0.00294715 0.04268765 22.12027881 7.65957825 CLK3 −1.001182295 0.00298754 0.04316491 116.5983771 64.7280973 EXOC6 1.462440669 0.00301764 0.0434915 18.62345299 52.863598 ETFA −1.033853587 0.00308391 0.04439151 211.4412429 133.8260265 RDH13 −1.827515919 0.00310699 0.04466828 13.62577429 4.675346 MTERF1 −1.58817958 0.00311175 0.04468131 27.75623086 14.80039655 LOC101927027 1.573304325 0.00312364 0.04474127 4.211369714 26.13527023 CNP −1.097403536 0.00313197 0.04480529 24.98781305 14.29346375 ARFGEF2 1.241620144 0.00313644 0.04481405 5.907125638 15.1121541 MCM3AP 1.160487177 0.00316806 0.04509921 18.98804667 42.63157293 SFPQ 1.206824864 0.00318158 0.04523452 47.61741619 88.602034 RAD9A −1.374057685 0.00318536 0.04523452 8.711004286 4.91682275 DAXX −1.06083514 0.00319593 0.04527372 30.36822905 19.98259299 LOC100996286 −1.83607122 0.00319235 0.04527372 60.76818519 17.25592475 TMEM237 −1.951065251 0.00328224 0.04630322 4.673383062 4.04188854 ARID1A 1.387569106 0.00331076 0.0463145 9.368136124 34.768239 CPT2 −1.139351315 0.00329723 0.0463145 30.83033438 21.19235005 ABT1 −1.19573184 0.00331435 0.0463145 132.1675667 74.13886765 TXK −1.569311232 0.00331402 0.0463145 43.40230785 18.92011706 LRRC45 −1.891079088 0.00329804 0.0463145 8.30033181 3.13344467 ZNF280C 1.36949936 0.00332362 0.04634687 2.081992714 10.13766255 MAP2K4 1.31117468 0.00333665 0.04636151 26.25060724 72.47427745 TUBB 1.097554669 0.0033353 0.04636151 17.42295328 33.04663 PLEKHA1 −1.045263518 0.003329 0.04636151 54.94448481 32.1948511 BBS5 1.096834176 0.0033706 0.04666548 3.122230367 9.2493915 GDAP2 1.290313972 0.0034067 0.04705306 6.706050762 13.2542057 AHR 1.102241377 0.00342225 0.04715557 7.888413095 13.6936725 TNFRSF9 −1.198960983 0.00342212 0.04715557 13.75290395 7.85170705 MCOLN2 1.245703069 0.00346294 0.04760314 21.82523003 47.5998025 XYLT1 1.99585507 0.00348152 0.04780194 0.782486952 3.604668105 PLCG2 −1.227346251 0.00350186 0.04797513 11.47080835 4.67658275 PRKAB1 −1.348576658 0.00350273 0.04797513 58.58896667 38.1610866 TGFBR1 1.130381688 0.00353755 0.04825283 17.15331511 33.0276235 U2AF1L4 −1.053266078 0.00353573 0.04825283 72.834 42.5640561 SNHG7 1.243507546 0.00355025 0.04834502 4.981000238 18.98669895 PPIL4 1.13138956 0.00361378 0.04915252 53.22107343 109.675935 NEK8 −1.427122553 0.00362368 0.04917181 5.678052524 2.17769965 PIK3R2 −1.504052614 0.0036227 0.04917181 4.701518429 1.342299575 MRPS18B −1.160658918 0.00364157 0.04934871 19.64079799 9.98160735 ABHD17B −1.180701066 0.00365373 0.04934871 12.83117357 8.93513765 ZFAND1 −1.35854761 0.00365182 0.04934871 50.26811962 28.80094995 TNFAIP8L2 −1.853922479 0.00369231 0.049754 57.96041905 14.49336125

TABLE 4 List of genes differentially expressed between Tumor TRM compared to Tumor non-TRM Mean TPM Mean TPM Common genes Gene ID log2FoldChange pvalue padj Tumor non-TRM Tumor TRM with Lung ITGAE 3.740189693 3.12E−72 3.85E−68 33.0593668 407.095 Yes S1PR5 −4.523578131 7.84E−34 4.84E−30 61.60472972 1.679153684 Yes GSG2 3.635833487 2.36E−28 7.27E−25 0.43646014 10.79137868 Yes GZMB 1.997567017 5.45E−28 1.34E−24 2123.78152 6599.232632 S1PR1 −3.027979011 9.21E−25 1.89E−21 191.05454 26.11258247 Yes MYO7A 3.513567966 5.58E−18 8.61E−15 2.571285816 44.23771526 Yes FOS −1.067494599 5.56E−18 8.61E−15 2389.31976 1583.288684 GPR25 3.483981326 7.12E−17 9.38E−14 4.76494824 85.23201053 Yes PLAC8 −2.474542579 7.61E−17 9.38E−14 55.3151272 10.19808989 Yes LAYN 3.621535741 1.31E−16 1.47E−13 1.848024708 52.53625789 KRT86 3.381632722 4.46E−16 4.58E−13 3.71512388 90.02109042 Yes STMN1 1.366049689 7.63E−16 6.72E−13 85.79118 171.0376579 PDCD1 1.57324167 1.08E−15 8.87E−13 86.55487464 225.5345789 Yes RBPJ 1.633587013 1.51E−15 1.16E−12 68.462808 235.5415263 Yes TCF7 −1.919812967 7.00E−15 4.80E−12 54.29527692 16.48907895 KLRG1 −2.072161115 6.62E−15 4.80E−12 487.1466948 115.8208 Yes ENTPD1 2.51222904 1.22E−14 7.94E−12 9.068538 51.02960526 ZNF683 2.168329213 1.38E−14 8.50E−12 146.606496 728.6827368 Yes SPRY1 3.136164239 2.23E−14 1.31E−11 4.932731568 55.74590526 Yes CCR7 −2.22354341 2.48E−14 1.39E−11 227.195484 56.20075605 Yes KLRC2 2.347036307 7.93E−14 4.08E−11 42.90052344 170.3632789 FCGR3A −2.930611162 1.81E−13 8.59E−11 119.760642 14.86318363 Yes ALOX5AP 1.211931191 3.98E−13 1.82E−10 709.35508 1504.629105 TOX 1.04690617 2.72E−12 1.20E−09 52.981492 111.2167158 TNS3 2.871610018 1.26E−11 5.17E−09 1.578477292 18.24837105 SRGAP3 2.957823113 1.36E−11 5.42E−09 1.467354292 21.03388737 Yes CCDC109B −1.149921845 1.41E−11 5.44E−09 373.868636 168.8402895 CLNK 2.981297463 1.56E−11 5.82E−09 3.97429732 48.79801947 Yes AFAP1L2 2.730768148 2.03E−11 7.35E−09 10.19192002 48.02176947 Yes SELL −2.397423991 2.35E−11 8.05E−09 442.230628 72.96528086 Yes GZMK −1.337577522 2.50E−11 8.33E−09 3386.79804 1582.416847 Yes IL7R −1.39682464 2.89E−11 9.39E−09 377.3089102 176.2078421 KLRC1 2.528735 4.60E−11 1.42E−08 32.23271408 201.5704368 Yes GOLIM4 2.325048002 5.07E−11 1.53E−08 22.3738512 81.66201579 Yes CXCL13 2.786215532 7.81E−11 2.15E−08 200.4329206 1830.654842 AKAP5 2.119777167 7.69E−11 2.15E−08 3.498434 12.78739474 HAVCR2 2.115630958 8.47E−11 2.22E−08 71.16694344 334.6920737 RASSF3 −1.294121912 1.08E−10 2.77E−08 88.174812 37.24806632 KIR2DL4 2.739112566 1.92E−10 4.82E−08 6.891746048 90.73159421 CD63 1.060494213 2.91E−10 7.19E−08 305.35808 549.2277895 PHLDA1 1.92802647 3.62E−10 8.76E−08 20.7194988 72.30838947 Yes CHRM3-AS2 2.773184838 4.89E−10 1.16E−07 2.12001284 45.31885842 ATP8B4 2.747605956 5.80E−10 1.30E−07 1.198385096 13.04629837 Yes TNFRSF9 2.018972638 6.12E−10 1.35E−07 17.28012864 75.16161053 CSF1 2.169258026 1.45E−09 3.04E−07 18.68793199 83.02554737 FGR −2.174444347 1.89E−09 3.88E−07 81.8702808 11.86666842 Yes RAB27A 1.270864982 2.44E−09 4.85E−07 72.8336226 165.1855579 FLOT1 −1.198609437 2.42E−09 4.85E−07 19.8708108 9.655719 PLEK −1.920067488 2.66E−09 5.21E−07 189.283604 41.99313458 Yes KLF3 −2.373844864 2.87E−09 5.54E−07 25.2746158 4.824050537 Yes DAPK2 2.020364656 3.05E−09 5.79E−07 15.41775706 64.87444211 Yes FAM3C 1.645072966 3.50E−09 6.55E−07 33.074066 118.3906789 LINC00861 −1.200761735 3.95E−09 7.27E−07 131.4451524 53.62909526 ARHGAP11A 1.788648298 4.28E−09 7.77E−07 3.261430284 10.31301842 Yes RRM2 2.032567257 4.49E−09 8.03E−07 24.39809328 51.55875105 ETV1 2.431245366 7.54E−09 1.31E−06 1.990125728 17.19050579 Yes CD109 2.209733753 8.57E−09 1.47E−06 1.058001288 4.155530368 CD7 1.243952111 9.59E−09 1.62E−06 152.706784 335.0916316 DBH-AS1 2.446850699 1.11E−08 1.84E−06 1.18816808 9.190445 TMIGD2 1.755365521 1.23E−08 2.02E−06 12.8405268 46.83354474 Yes SIRPG 1.173047291 1.38E−08 2.24E−06 185.4196008 477.9031579 SORL1 −1.620313508 1.63E−08 2.54E−06 29.9296156 11.53147181 Yes DOCK5 2.249256949 1.83E−08 2.81E−06 1.279708636 3.712515426 Yes CXCR6 1.209548172 2.19E−08 3.30E−06 639.847916 1379.978 Yes CCL3 1.308047955 2.27E−08 3.37E−06 448.815904 879.4891053 FAM65B −1.285468642 2.86E−08 4.21E−06 67.4624064 25.83885526 Yes KIF2C 2.340084386 3.24E−08 4.65E−06 2.716626244 15.13782305 CD101 2.149991882 4.52E−08 6.27E−06 10.88423144 46.95427195 PZP −1.943336483 5.16E−08 6.99E−06 11.83152352 3.274193368 Yes PAQR4 2.35562189 6.71E−08 9.00E−06 1.689015624 10.24887293 KLRF1 −2.393123248 1.07E−07 1.41E−05 69.15205336 3.035728737 Yes XCL1 1.366128861 1.27E−07 1.64E−05 39.7461872 96.09117895 Yes CAPG 1.119966807 1.59E−07 2.03E−05 231.99522 437.7557895 Yes WBP4 1.209997096 1.74E−07 2.17E−05 29.181684 35.85908947 IVNS1ABP 1.261438848 1.82E−07 2.25E−05 128.6844636 275.7747368 Yes ADAM19 1.007948314 2.23E−07 2.70E−05 23.6940676 53.16917895 Yes DHRS3 −1.538676923 2.94E−07 3.52E−05 150.522892 53.74266979 CTLA4 2.049857964 3.17E−07 3.76E−05 50.97031992 243.8928526 Yes CLIC3 1.098704683 4.25E−07 4.77E−05 58.91024892 122.7453368 FCGR3B −2.089580696 4.25E−07 4.77E−05 15.66373078 1.389735158 CX3CR1 −2.269155106 4.32E−07 4.80E−05 62.33696111 1.737185411 Yes RASA3 −1.805687466 5.15E−07 5.62E−05 54.1342792 11.73182377 Yes IFITM10 2.259689528 5.26E−07 5.64E−05 1.272724276 11.72409947 C1orf21 −1.891368092 7.09E−07 7.41E−05 11.4573743 2.148275695 Yes ATM −1.005296267 7.86E−07 8.15E−05 19.96884244 11.57904211 A2M −1.606407971 9.37E−07 9.64E−05 46.2580028 18.43226479 Yes GEM 2.209029285 1.15E−06 0.000114883 2.765744876 25.92595789 RASGRP2 −1.596008125 1.16E−06 0.000115288 51.92487504 14.19080942 Yes RAD51AP1 2.184111701 1.17E−06 0.00011594 2.22739396 17.47792795 KIFC1 2.048040416 1.19E−06 0.000116485 1.139757476 2.437878232 PTMS 1.556394667 1.24E−06 0.000120768 19.1542684 50.72354737 Yes UBASH3B 1.896874413 1.37E−06 0.000131202 3.828418912 14.88145069 NUSAP1 1.471883402 1.54E−06 0.000146458 50.15568704 89.48275263 CD300A −1.793079022 1.74E−06 0.000162542 90.8459596 25.90228042 Yes TPX2 1.99097788 1.92E−06 0.000178166 4.851452292 24.50750737 AURKA 1.660078116 2.03E−06 0.00018727 17.55234268 26.20345458 KIF5C 1.774252318 2.20E−06 0.000197891 2.414951648 7.161932421 Yes VDR 1.916130686 2.32E−06 0.000204746 3.135480952 10.44611358 SYNJ2 1.952272272 2.36E−06 0.000206522 1.49190332 4.358019632 ATP10A 1.719330489 2.48E−06 0.000213579 1.708745156 6.061125495 ANKRD35 1.989093325 2.82E−06 0.00024034 2.90909116 12.88178263 KLRC3 1.732307354 3.39E−06 0.000282668 38.1205408 104.6819184 SCCPDH 1.267108566 4.37E−06 0.000357373 42.22486096 81.38209368 KIAA0101 1.705284926 5.92E−06 0.000467234 34.92798628 57.27524105 Yes CHN1 1.976034237 5.99E−06 0.000467565 8.04594322 65.83489663 Yes GTSE1 1.845443965 6.08E−06 0.000471895 2.67869814 5.922737205 ICAM2 −1.574882829 6.48E−06 0.000499591 51.860032 16.69807895 Yes TTC24 1.676924085 7.49E−06 0.000570348 15.26772828 48.94895789 ZC3H12C 2.032896561 7.69E−06 0.000578224 0.060009268 1.357966053 DKK3 −1.679421385 8.50E−06 0.000631421 85.16482245 33.83001479 SARDH 1.907804454 1.06E−05 0.000768356 10.17270494 48.39059842 Yes ZBED2 1.958522644 1.07E−05 0.000775184 8.41407728 44.29435947 DPF3 1.994370435 1.10E−05 0.00078556 0.155604456 4.017322021 ARHGEF12 1.884195097 1.15E−05 0.000815589 1.720978192 6.359689242 CHEK1 1.633053468 1.15E−05 0.000815589 1.72233128 6.573517947 SLC2A8 1.898875877 1.19E−05 0.000828911 5.26762768 32.67995121 PLAGL1 1.977412408 1.25E−05 0.00086325 0.678131668 8.558824895 IL15 −1.776336936 1.39E−05 0.000953245 8.955241728 1.929823932 CXCL16 −1.465177681 1.42E−05 0.000968842 33.54755604 13.30089653 LILRP2 1.929179014 1.72E−05 0.00115957 0.24319804 8.555959895 UAP1L1 1.635659609 2.06E−05 0.001350032 4.842522948 10.79838142 Yes EOMES −1.082992213 2.14E−05 0.001392357 216.4456344 112.0899995 Yes XCL2 1.022838013 2.27E−05 0.001461407 117.1022852 217.8741316 FANCI 1.767956945 2.29E−05 0.001462592 5.771166696 18.30659889 CDT1 1.563740306 2.48E−05 0.00157686 2.84855772 6.826375684 CACNA2D2 −1.806533258 2.50E−05 0.001578903 1.939408892 0.800522095 TNFSF4 1.864855811 2.57E−05 0.001607152 8.7365456 41.49277777 ABAT 1.714711942 2.59E−05 0.001607152 1.998721984 5.892387211 AHI1 1.38245204 2.59E−05 0.001607152 12.14165304 27.49229789 Yes ASB2 1.517671152 2.61E−05 0.00161221 13.9961398 43.76039474 DBN1 1.423942324 2.66E−05 0.001630949 8.21897794 19.68978158 LGMN −1.534420984 2.72E−05 0.001663415 51.97189676 13.32886032 PTGIS 1.360710073 3.22E−05 0.001920191 1.0126632 4.015272263 DFNB31 1.751477613 3.52E−05 0.00204918 2.68782576 11.29693105 Yes ABCB1 1.289757669 3.51E−05 0.00204918 17.53565533 46.09189158 ATP10D 1.408338045 3.94E−05 0.002261703 8.2785482 18.62304158 Yes FCRL3 −1.634974976 3.94E−05 0.002261703 57.74915068 22.82451037 Yes SPINT2 −1.670867169 4.03E−05 0.002304224 81.86661636 19.88341084 LYZ −1.206783578 4.12E−05 0.0023407 533.8098288 197.5742774 GCNT1 1.590291886 4.33E−05 0.002452984 8.729126624 25.69184137 NUAK2 −1.164676934 4.57E−05 0.002563473 15.3906328 5.075766368 Yes SPNS3 1.583706166 4.60E−05 0.002564261 9.59597132 25.19064737 PTGDR −1.106484699 4.61E−05 0.002564261 47.7035032 22.43134053 SOCS3 −1.176968482 4.94E−05 0.00271839 130.4092708 54.63775147 KLRAP1 −1.668786132 4.93E−05 0.00271839 31.6027852 8.668786158 Yes RUNX2 1.004705254 5.13E−05 0.002789748 21.72283 46.77662105 SVIL −1.730549769 5.18E−05 0.002803752 6.2219778 0.869931489 Yes KLRB1 1.243451233 5.22E−05 0.002809517 249.3302182 490.05 LINC00963 1.231289717 5.46E−05 0.002923658 15.691546 35.08958263 AMICA1 1.140277208 5.47E−05 0.002923658 158.6033022 366.6787895 Yes CAMK1 1.691816551 5.89E−05 0.00313345 12.76232984 54.144063 ANKS1B 1.817118618 5.94E−05 0.003144996 0.373814604 2.183310789 TMEM200A 1.542695194 6.20E−05 0.003235193 10.90489994 25.15744737 Yes PGLYRP2 1.516783203 6.17E−05 0.003235193 2.53230324 5.937835789 Yes TTN-AS1 −1.265199771 6.26E−05 0.003235193 3.374815664 1.286795842 PRSS23 −1.409768113 6.38E−05 0.003281671 9.47118448 1.732799947 Yes SUOX 1.791011507 6.48E−05 0.003317961 2.148762428 16.12955742 KCNK5 1.782686156 6.88E−05 0.003491967 3.473615356 20.51417421 DIXDC1 1.621869122 7.38E−05 0.003717195 0.632839116 3.195448053 CD28 −1.118957638 7.48E−05 0.003749953 77.15902102 30.25053684 KIF14 1.61077793 7.88E−05 0.003902254 0.70509062 3.265626621 SNAP47 1.087540861 8.59E−05 0.004188653 45.1029988 73.56212632 ABCA1 −1.727318199 9.08E−05 0.004411705 4.982207032 0.730471247 ITGA5 −1.378404403 9.24E−05 0.004468571 36.65399692 11.21532078 Yes SYNGR3 1.663443022 0.000110901 0.005290731 10.13336744 42.42283158 RBBP9 1.35864834 0.000116888 0.0054847 3.0743766 7.297156368 SATB1 −1.222543971 0.000116923 0.0054847 21.52242938 10.02693033 Yes C1orf106 1.654127927 0.000117558 0.005493609 0.429621136 2.195005284 DENND4A −1.058190967 0.000120038 0.005546478 22.5608188 12.16038737 FASLG 1.059205283 0.000127409 0.00586509 149.7843665 263.5392211 RGS16 1.695730781 0.000132589 0.006058347 9.466770612 44.55431789 CCNA2 1.63474981 0.000137262 0.006225719 16.76836842 30.29312263 SLC16A6 1.705711712 0.000139293 0.006263427 1.215146864 7.487247474 BAZ2B −1.004936545 0.000139515 0.006263427 22.017383 11.41999521 CCRL2 1.61789572 0.000142927 0.006388745 18.98001112 41.17150789 COL6A2 −1.68505588 0.000143773 0.006403357 5.798380532 0.906108884 PXN −1.349346038 0.000144295 0.006403473 60.2272196 19.01642169 GINS1 1.561048491 0.000151389 0.006694195 2.35536462 9.327755947 ACP5 1.360154914 0.000160861 0.007023499 68.29268804 166.9910632 BCL2L11 1.353352837 0.000161682 0.007023499 6.783699552 20.68451776 USP14 1.141451467 0.000161566 0.007023499 37.82807232 60.20166842 MIR155HG 1.568103062 0.000171693 0.007354789 17.03474912 61.13262474 Yes TK1 1.556990809 0.000172831 0.007377908 34.81022376 62.27577368 BIRC5 1.52789537 0.000175728 0.007475706 17.88640092 21.82443158 CRIP2 −1.682549856 0.000176976 0.007502923 10.84884325 1.230886568 TTYH3 1.550941892 0.000194697 0.00814229 1.855617216 6.575156084 CASC5 1.683852608 0.000202408 0.008379571 0.7152126 6.408608889 HELLS 1.194430839 0.000203562 0.008399136 6.51769844 11.66888526 NHS 1.677210619 0.000219388 0.008962238 0.385406112 3.465654453 CENPU 1.383262737 0.000221289 0.008983776 8.1349832 21.60639474 AMY2B −1.142210726 0.000221372 0.008983776 10.3340532 4.278311474 BRCA1 1.317781531 0.000223203 0.009028397 1.784135444 4.474544053 PDLIM7 1.491977557 0.00022575 0.009101574 10.8833996 31.69171263 FAM84B −1.321001087 0.000243726 0.009762507 9.7799954 3.669650684 C15orf53 1.602472447 0.000248983 0.009940784 3.78734548 11.796093 Yes HHLA3 −1.453124988 0.000260044 0.010282577 26.8391892 7.859869105 Yes INPP5F 1.557089828 0.00026203 0.010295127 4.56110998 14.65299721 Yes APEX2 1.150826136 0.000317738 0.012326832 26.07538084 42.41617368 ADRB2 −1.371983994 0.000317481 0.012326832 116.3136846 40.39521814 Yes THEM4 −1.136585144 0.000331488 0.012779916 22.07544464 9.964039 MCM2 1.417554569 0.000357578 0.013573646 12.7239374 34.84616211 PDLIM1 −1.20806686 0.000356537 0.013573646 60.56773384 26.39405689 HJURP 1.512082809 0.000361013 0.013662027 3.0432999 10.34542453 RDH10 1.392983915 0.000374495 0.014000421 4.7784252 13.67609332 FUT8 1.270544325 0.000388012 0.014375073 16.45712298 39.12060211 MKI67 1.482800913 0.000403173 0.01475575 3.740788684 9.95709 Yes CDHR3 −1.220422843 0.000409235 0.014893018 4.32965516 1.594811011 RYBP 1.530157948 0.000426073 0.015369766 1.76679696 6.359807421 MYO1E 1.582425887 0.000450856 0.016029436 3.620583316 15.26436368 TOP2A 1.352846874 0.000459462 0.016288455 14.67442033 32.63963384 NDFIP2 1.116525313 0.000467494 0.016478506 40.7288212 91.40315263 MAD2L2 1.121501594 0.00048711 0.017121003 80.19018184 176.2801105 BRCA2 1.417780448 0.00049781 0.017436904 1.666127432 4.232477479 WDTC1 −1.383667482 0.000498958 0.017436904 7.652969292 3.021814247 ITGA1 1.132457545 0.000525477 0.018037457 53.19740808 109.6885368 Yes PLEKHG3 −1.568835408 0.0005408 0.018481593 7.94662748 0.826099621 Yes MAP3K6 1.405480363 0.000557245 0.018886615 2.83304806 7.054325263 AASS −1.263208166 0.000575905 0.019306886 11.31210144 2.225013795 IL18BP −1.194180888 0.000582721 0.019482467 54.67915012 21.38025753 RGS12 −1.099836473 0.000595214 0.019792869 3.29526236 0.982810789 CD14 −1.424675215 0.000606273 0.02010642 67.70359144 14.33756879 Yes KRT81 1.555202248 0.000612805 0.020127568 2.24269256 18.08521053 GALNT2 1.207810735 0.000613436 0.020127568 21.1598464 43.74474211 MPST 1.049224887 0.000613323 0.020127568 22.35174136 37.2394 PATL2 −1.117057136 0.000612364 0.020127568 85.39035448 37.18099205 Yes CEP41 1.008732155 0.000633389 0.020727119 3.8788086 8.499325737 ELK1 1.299109563 0.000643899 0.021015287 10.29477778 20.22793895 PPP1R21 1.01536727 0.000714978 0.022910882 21.55712479 36.39565316 DHFR 1.364150744 0.000717621 0.022935974 6.949739276 16.97895126 CCDC50 1.32032169 0.000721961 0.023015076 4.642402768 8.387248079 PDE7B 1.258502263 0.0007291 0.023019759 1.530637224 4.094892895 BMPR1B −1.192565663 0.000729572 0.023019759 2.2447436 0.246532374 CCL18 −1.43447936 0.00074364 0.023344239 120.7602916 32.81444384 KIAA1524 1.421479171 0.000758263 0.023742862 4.381154288 13.40530519 ATL2 1.147738802 0.00079133 0.024345745 7.3282878 13.66836579 Yes CARD6 1.29906251 0.000813151 0.024892909 1.37840932 6.053855789 ZNF514 −1.43484038 0.000832933 0.025372588 5.027664092 0.490173568 AGPS 1.217710921 0.000852721 0.025776769 7.239435836 16.10707737 C1QC −1.289800192 0.000850368 0.025776769 122.636079 59.72089795 CDKN2C 1.303116031 0.000901495 0.026984604 11.7002326 27.63487263 CD200R1 1.029994841 0.000903351 0.026984604 45.1439298 88.27228947 SLC4A2 1.214325475 0.000956432 0.027894817 7.793165208 15.40505526 ACSL4 1.044437072 0.000960786 0.027955697 19.3627672 34.15788474 Yes LOC101928988 1.400426941 0.000984987 0.028458522 5.24736404 13.66175105 CDC6 1.282112236 0.000983324 0.028458522 4.7555254 6.325555684 LOC100996286 −1.376533152 0.001018637 0.029157611 60.23214176 18.27346626 Yes C1orf162 −1.321986663 0.001023341 0.029224437 149.498455 55.57553158 LRRN3 1.465688528 0.001065437 0.030286388 2.271649976 29.283488 TET2 1.119528008 0.001084458 0.030756213 6.91857828 11.33880421 ZWINT 1.400223352 0.001092088 0.030901593 19.685767 40.97230947 TNFRSF18 1.436215773 0.001123689 0.031578469 20.86098484 73.40014211 APOE −1.254820747 0.001173695 0.032612345 125.2921753 55.44808074 RHOC 1.010556359 0.001187599 0.032850683 64.81549196 137.4523789 PTP4A3 −1.470184527 0.001202261 0.033181871 10.16620279 1.326568737 PALB2 1.441779748 0.001209152 0.033258812 3.711650348 11.35856868 RAP1GAP2 −1.46757526 0.001210441 0.033258812 3.335396492 0.336865295 Yes PMM2 1.057768261 0.001233653 0.033789307 20.37359668 30.27954895 EPSTI1 1.148188972 0.001238521 0.033804499 20.8874532 45.41434368 WIPF3 1.394904839 0.001249282 0.033969958 4.911925736 18.05181664 PLAUR −1.41499774 0.00125009 0.033969958 88.264021 20.07343137 Yes LINC00539 1.398270111 0.00126082 0.034111277 12.49131944 34.69323995 LIMK1 1.300030117 0.00127323 0.034296578 9.1342948 23.865563 MAN1A1 1.085325998 0.00130679 0.034847713 10.56250701 21.17127137 SAC3D1 1.302130097 0.0013855 0.036523325 5.48930056 16.32300211 CKAP2L 1.321005047 0.001420909 0.036776193 3.42151756 7.193064868 NAIF1 1.139889907 0.001417708 0.036776193 1.77297092 4.622296579 AMZ1 1.401084929 0.001430853 0.036835065 0.757580336 4.697318947 ZDHHC18 1.322634784 0.001433155 0.036835065 3.679985244 8.532325263 CST3 −1.206589704 0.001485587 0.038024247 54.298094 30.77291768 Yes SDHAP1 1.169568814 0.001490256 0.038064774 4.97298268 9.654161053 SSH1 1.136181425 0.001535372 0.03886269 4.366832536 8.201638421 GINS3 1.431414228 0.001595634 0.039929684 0.8409542 6.411405879 NAV1 −1.182098287 0.001601388 0.039940807 1.522865816 0.5266027 CASP9 1.19556286 0.001614154 0.040148821 9.795832856 11.05799316 SGK1 −1.003855752 0.001621156 0.040241856 59.85603994 29.82423195 ITGA2 1.216143898 0.001632734 0.04036681 3.19618754 5.027070905 MZB1 1.309894553 0.001693951 0.041547254 24.83474744 56.75498526 KLF2 −1.23514898 0.001707128 0.041626658 56.41764416 25.34566311 VCAM1 1.142380045 0.001725703 0.041909455 44.44741996 77.7627 TIAM2 1.421865316 0.001741213 0.042203047 0.52895268 3.874846842 SLC27A2 1.267279008 0.001756777 0.042334312 31.71734129 74.47079316 Yes ST8SIA1 −1.004763422 0.001756924 0.042334312 6.43101508 3.163632211 PIAS2 −1.272095252 0.001754315 0.042334312 10.40526076 3.268512858 XYLT1 1.365509161 0.001779998 0.042806697 1.1606454 2.969577158 Yes ADAMTS17 1.146044925 0.001798084 0.043073725 0.970106168 2.848200895 PLEKHA5 −1.028909852 0.001810506 0.043287231 8.6564934 3.563287947 IL18RAP 1.246342812 0.001856197 0.043892409 30.24285953 66.06705263 ACOT7 1.351789221 0.001882395 0.044403647 13.74920072 39.01015632 TYROBP −1.130633801 0.001971061 0.046142286 286.6825624 111.0874333 SNX9 1.194321551 0.001980146 0.046267155 18.41411002 40.61670526 GPA33 1.400729896 0.001984058 0.046270937 4.352248768 17.61862789 UHRF1 1.171771134 0.002007933 0.046651354 4.08198472 9.493753579 GMNN 1.30781058 0.002014754 0.046721835 40.01412236 62.66041737 CDCA3 1.390821103 0.002025511 0.046883172 2.010761628 9.040348842 RMND1 1.120686463 0.002045247 0.047251337 14.60276136 22.08568263 PDE4A 1.129148503 0.002055599 0.047401735 3.8111694 7.638657368 Yes TRIM16 −1.221863915 0.00210131 0.048185618 2.71523592 0.879359011 RIC1 1.123345605 0.002115833 0.048428633 4.893008408 9.940590942 CDKN3 1.30287349 0.002126804 0.048499774 33.00784028 71.41053842 KCTD9 1.333185286 0.002141187 0.048558511 6.9347676 29.03811263 EMC9 1.213045927 0.00213996 0.048558511 29.22870432 55.71777368 NUDT14 1.068661519 0.00215483 0.048778243 46.7673308 83.1857 SLC18A2 −1.346962778 0.002163723 0.048800448 16.25625348 1.404258258 CD226 1.147445623 0.00217143 0.048810471 23.20268566 55.71804068 Yes AZIN2 1.373051169 0.002208751 0.049097951 3.109285076 13.45753084 CDK1 1.312177938 0.002227322 0.049332993 15.2390608 34.75512211

TABLE 5 List of genes uniquely expressed in Tumor TRM Gene ID log2FoldChange-vs- log2FoldChange-vs- log2FoldChange-vs- Lung-Non-TRM Lung-TRM Tumor-Non-TRM GSG2 5.217896122 1.727403278 3.635833487 MYO7A 11.04811002 2.259998486 3.513567966 LAYN 7.695794138 4.310357629 3.621535741 KRT86 6.065996193 2.036854575 3.381632722 STMN1 1.440509864 1.315548427 1.366049689 ENTPD1 3.816873481 3.015149779 2.51222904 KLRC2 2.854407583 1.140028446 2.347036307 TOX 1.63476055 1.232637535 1.04690617 TNS3 3.760885198 3.497445411 2.871610018 SRGAP3 6.94255386 2.092844412 2.957823113 CLNK 8.145947295 1.421442605 2.981297463 AFAP1L2 6.701024419 3.220993336 2.730768148 AKAP5 3.365061373 2.50622053 2.119777167 CXCL13 5.905835106 6.008906489 2.786215532 HAVCR2 2.830747425 2.50361094 2.115630958 KIR2DL4 5.015048432 2.424076288 2.739112566 CHRM3-AS2 2.363451642 2.414451417 2.773184838 TNFRSF9 2.408977601 3.457216272 2.018972638 RRM2 3.354352762 2.459896628 2.032567257 CD109 2.569939564 1.712328303 2.209733753 DBH-AS1 3.363084813 2.112220802 2.446850699 SIRPG 1.906534643 2.9228103 1.173047291 CCL3 1.367188475 1.746678602 1.308047955 KIF2C 4.589213359 3.985938676 2.340084386 CTLA4 3.667516869 2.138134252 2.049857964 IFITM10 3.604262638 2.307516511 2.259689528 GEM 3.079089548 4.530445048 2.209029285 RAD51AP1 2.090366569 3.053062158 2.184111701 KIFC1 2.481368317 2.80215291 2.048040416 NUSAP1 2.851989004 1.698825624 1.471883402 AURKA 3.109026783 2.744353884 1.660078116 VDR 1.95356489 2.683453148 1.916130686 KIAA0101 3.295801712 1.951171749 1.705284926 ZC3H12C 3.7603296 3.563030404 2.032896561 ZBED2 5.239224614 4.760873221 1.958522644 LILRP2 4.430404492 3.045769331 1.929179014 FANCI 1.852185073 2.161761079 1.767956945 TNFSF4 3.517317663 2.849440698 1.864855811 ASB2 3.153886441 2.744017787 1.517671152 CAMK1 2.833585603 2.144045067 1.691816551 ANKS1B 2.773441919 3.802723742 1.817118618 SUOX 2.133564156 2.455137719 1.791011507 KCNK5 4.46330601 2.53092669 1.782686156 KIF14 2.380994181 2.894778174 1.61077793 SYNGR3 7.183233568 2.03673754 1.663443022 C1orf106 1.817676184 2.317507122 1.654127927 CCNA2 2.582220087 4.786563234 1.63474981 CCRL2 1.868021117 1.993576214 1.61789572 GINS1 2.672407184 2.308923834 1.561048491 TK1 2.003932836 2.116056331 1.556990809 BIRC5 2.035908601 2.086973635 1.52789537 CASC5 4.535303428 2.610124315 1.683852608 INPP5F 4.725026767 2.536080077 1.557089828 MCM2 2.195434017 1.931278407 1.417554569 HJURP 4.247955681 2.482548456 1.512082809 RDH10 1.465733095 1.575939474 1.392983915 FUT8 2.205316587 1.36327465 1.270544325 MKI67 5.540218705 2.619692627 1.482800913 MYO1E 5.860674228 3.123013282 1.582425887 TOP2A 2.592103178 2.29539434 1.352846874 NDFIP2 2.713458817 1.680758608 1.116525313 PDE7B 2.536531597 2.200650041 1.258502263 CDC6 2.141006594 1.532873841 1.282112236 LOC1019289 3.758323048 1.797657182 1.400426941 TNFRSF18 1.978937742 1.965403844 1.436215773 WIPF3 4.099033144 3.849918125 1.394904839 CKAP2L 2.704714791 1.999536687 1.321005047 AMZ1 4.135576107 2.360849448 1.401084929 ITGA2 3.554125945 2.137378038 1.216143898 MZB1 3.47569117 2.399099383 1.309894553 VCAM1 3.276880266 2.615175589 1.142380045 TIAM2 2.708362955 2.731877282 1.421865316 SLC27A2 3.492322458 1.81432632 1.267279008 UHRF1 2.369255423 2.106619252 1.171771134 RIC1 1.504751576 2.018930511 1.123345605 EMC9 1.162931851 2.837975396 1.213045927 CDK1 2.597647929 1.787691839 1.312177938 PLAC8 −3.317514759 −1.464685708 −2.474542579 FCGR3A −5.118125315 −3.03735392 −2.930611162 KLRF1 −7.586330442 −2.531583712 −2.393123248 DHRS3 −1.984899007 −1.576886916 −1.538676923 FCGR3B −4.595506208 −3.242872284 −2.089580696 CXCL16 −3.57727385 −2.899486306 −1.465177681 LYZ −3.456823139 −2.949409285 −1.206783578 SVIL −3.938867287 −2.316019064 −1.730549769 PXN −2.85790707 −2.148037695 −1.349346038 CRIP2 −3.769688421 −4.197779904 −1.682549856 CCL18 −3.20866263 −2.603917856 −1.43447936 C1QC −3.265559876 −2.572827616 −1.289800192 C1orf162 −2.648782815 −2.037386914 −1.321986663 PLAUR −4.779211289 −2.670399998 −1.41499774 TYROBP −2.281337445 −2.008092934 −1.130633801 pvalue-vs- pvalue-vs- pvalue-vs- Lung-Non-TRM Lung-TRM Tumor-Non-TRM GSG2 5.02E−26 0.000758938 2.36E−28 MYO7A  3.47E−107 1.34E−05 5.58E−18 LAYN 8.56E−39 5.03E−13 1.31E−16 KRT86 7.30E−21 0.000900314 4.46E−16 STMN1 2.16E−08 0.000695078 7.63E−16 ENTPD1 2.22E−27 1.36E−18 1.22E−14 KLRC2 5.53E−10 0.002517694 7.93E−14 TOX 2.55E−10 1.09E−06 2.72E−12 TNS3 5.89E−09 2.77E−09 1.26E−11 SRGAP3 5.11E−32 0.000112977 1.36E−11 CLNK 7.74E−35 0.001945149 1.56E−11 AFAP1L2 5.37E−33 4.88E−08 2.03E−11 AKAP5 2.16E−22 1.17E−13 7.69E−11 CXCL13 1.94E−16 6.94E−23 7.81E−11 HAVCR2 2.47E−13 2.37E−10 8.47E−11 KIR2DL4 1.17E−17 9.34E−05 1.92E−10 CHRM3-AS2 0.000321447 3.49E−06 4.89E−10 TNFRSF9 4.92E−10 1.38E−17 6.12E−10 RRM2 2.59E−12 2.37E−08 4.49E−09 CD109 5.35E−09 0.000244747 8.57E−09 DBH-AS1 9.85E−05 0.002055901 1.11E−08 SIRPG 2.69E−07 6.57E−13 1.38E−08 CCL3 1.14E−05 1.34E−07 2.27E−08 KIF2C 1.15E−10 2.19E−10 3.24E−08 CTLA4 7.06E−17 1.34E−07 3.17E−07 IFITM10 2.56E−08 8.36E−05 5.26E−07 GEM 0.000154922 3.22E−10 1.15E−06 RAD51AP1 0.001952484 3.23E−05 1.17E−06 KIFC1 9.82E−05 6.16E−06 1.19E−06 NUSAP1 3.66E−07 5.28E−08 1.54E−06 AURKA 5.48E−14 1.75E−09 2.03E−06 VDR 0.000531623 5.48E−07 2.32E−06 KIAA0101 1.04E−16 2.33E−05 5.92E−06 ZC3H12C 6.05E−06 1.52E−06 7.69E−06 ZBED2 6.59E−13 1.64E−13 1.07E−05 LILRP2 4.75E−11 1.91E−06 1.72E−05 FANCI 1.65E−05 3.98E−05 2.29E−05 TNFSF4 6.96E−07 2.30E−07 2.57E−05 ASB2 1.09E−06 1.21E−08 2.61E−05 CAMK1 7.88E−07 0.000110133 5.89E−05 ANKS1B 0.00126077 1.57E−06 5.94E−05 SUOX 0.001029801 0.000762109 6.48E−05 KCNK5 4.16E−11 0.000251743 6.88E−05 KIF14 7.48E−07 2.19E−07 7.88E−05 SYNGR3 5.69E−33 0.001004625 0.000110901 C1orf106 0.001986359 0.000131969 0.000117558 CCNA2 7.12E−05 1.25E−13 0.000137262 CCRL2 0.005883769 0.002902786 0.000142927 GINS1 2.42E−10 1.62E−06 0.000151389 TK1 0.001539824 0.000225416 0.000172831 BIRC5 0.000671717 0.000761326 0.000175728 CASC5 7.91E−11 0.000117916 0.000202408 INPP5F 3.54E−14 7.06E−06 0.00026203 MCM2 8.88E−05 0.000448689 0.000357578 HJURP 2.82E−23 9.34E−07 0.000361013 RDH10 0.00736061 0.001570757 0.000374495 FUT8 4.52E−06 0.001561597 0.000388012 MKI67 5.85E−17 3.30E−05 0.000403173 MYO1E 1.73E−21 1.47E−06 0.000450856 TOP2A 9.06E−06 4.98E−05 0.000459462 NDFIP2 1.26E−08 0.000537766 0.000467494 PDE7B 1.36E−10 9.45E−06 0.0007291 CDC6 1.27E−06 0.000175457 0.000983324 LOC1019289 1.57E−08 0.001917135 0.000984987 TNFRSF18 0.001847341 0.000382211 0.001123689 WIPF3 2.68E−14 4.03E−14 0.001249282 CKAP2L 7.33E−06 0.000608346 0.001420909 AMZ1 2.88E−10 0.000875684 0.001430853 ITGA2 2.23E−16 0.000112489 0.001632734 MZB1 9.58E−10 3.77E−05 0.001693951 VCAM1 2.92E−08 2.67E−05 0.001725703 TIAM2 0.00031317 0.000187503 0.001741213 SLC27A2 1.53E−07 0.001644883 0.001756777 UHRF1 5.16E−09 3.57E−07 0.002007933 RIC1 0.008526297 0.001650318 0.002115833 EMC9 0.001576823 3.51E−07 0.00213996 CDK1 0.00030535 0.002941176 0.002227322 PLAC8 7.58E−15 0.002185141 7.61E−17 FCGR3A 1.41E−19 1.95E−07 1.81E−13 KLRF1 2.28E−44 0.001366298 1.07E−07 DHRS3 5.09E−12 0.000251292 2.94E−07 FCGR3B 1.28E−16 4.90E−08 4.25E−07 CXCL16 2.76E−21 2.41E−10 1.42E−05 LYZ 5.86E−25 3.71E−19 4.12E−05 SVIL 1.70E−17 1.93E−05 5.18E−05 PXN 5.41E−13 9.50E−07 0.000144295 CRIP2 6.48E−10 1.44E−15 0.000176976 CCL18 1.17E−07 0.000146058 0.00074364 C1QC 3.39E−13 1.62E−06 0.000850368 C1orf162 1.11E−09 3.06E−06 0.001023341 PLAUR 4.56E−19 2.63E−05 0.00125009 TYROBP 1.04E−06 1.71E−05 0.001971061 padj-vs- padj-vs- padj-vs- Lung-Non-TRM Lung-TRM Tumor-Non-TRM GSG2 3.35E−23 0.017682536 7.27E−25 MYO7A  4.39E−103 0.000734598 8.61E−15 LAYN 1.36E−35 2.07E−10 1.47E−13 KRT86 2.25E−18 0.020077613 4.58E−13 STMN1 8.43E−07 0.016592582 6.72E−13 ENTPD1 1.65E−24 1.99E−15 7.94E−12 KLRC2 3.14E−08 0.040436251 4.08E−11 TOX 1.51E−08 9.50E−05 1.20E−09 TNS3 2.72E−07 5.22E−07 5.17E−09 SRGAP3 5.39E−29 0.004201487 5.42E−09 CLNK 1.09E−31 0.034357992 5.82E−09 AFAP1L2 6.55E−30 6.81E−06 7.35E−09 AKAP5 9.43E−20 5.93E−11 2.15E−08 CXCL13 3.19E−14 4.58E−19 2.15E−08 HAVCR2 2.58E−11 5.90E−08 2.22E−08 KIR2DL4 2.47E−15 0.003617679 4.82E−08 CHRM3-AS2 0.003597258 0.000248846 1.16E−07 TNFRSF9 2.83E−08 1.83E−14 1.35E−07 RRM2 2.22E−10 3.64E−06 8.03E−07 CD109 2.51E−07 0.00737706 1.47E−06 DBH-AS1 0.001337201 0.035666244 1.84E−06 SIRPG 8.23E−06 2.63E−10 2.24E−06 CCL3 0.000214864 1.59E−05 3.37E−06 KIF2C 7.30E−09 5.68E−08 4.65E−06 CTLA4 1.28E−14 1.59E−05 3.76E−05 IFITM10 9.82E−07 0.003365354 5.64E−05 GEM 0.001972409 7.73E−08 0.000114883 RAD51AP1 0.015324702 0.00153112 0.00011594 KIFC1 0.001334843 0.000383892 0.000116485 NUSAP1 1.09E−05 7.26E−06 0.000146458 AURKA 6.25E−12 3.45E−07 0.00018727 VDR 0.005413661 5.18E−05 0.000204746 KIAA0101 1.85E−14 0.001161096 0.000467234 ZC3H12C 0.000126191 0.000125603 0.000578224 ZBED2 6.28E−11 7.45E−11 0.000775184 LILRP2 3.27E−09 0.0001461 0.00115957 FANCI 0.000295897 0.001812139 0.001462592 TNFSF4 1.95E−05 2.48E−05 0.001607152 ASB2 2.88E−05 1.90E−06 0.00161221 CAMK1 2.16E−05 0.004142382 0.00313345 ANKS1B 0.010841195 0.000127578 0.003144996 SUOX 0.00923904 0.017682536 0.003317961 KCNK5 2.93E−09 0.007520596 0.003491967 KIF14 2.07E−05 2.41E−05 0.003902254 SYNGR3 6.55E−30 0.021651416 0.005290731 C1orf106 0.015542433 0.004671714 0.005493609 CCNA2 0.001016184 6.01E−11 0.006225719 CCRL2 0.035886174 0.044354845 0.006388745 GINS1 1.44E−08 0.000129199 0.006694195 TK1 0.012782761 0.00696941 0.007377908 BIRC5 0.006540595 0.017682536 0.007475706 CASC5 5.14E−09 0.004265019 0.008379571 INPP5F 4.15E−12 0.000431737 0.010295127 MCM2 0.001228037 0.011894768 0.013573646 HJURP 1.32E−20 8.50E−05 0.013662027 RDH10 0.042395964 0.029400015 0.014000421 FUT8 9.81E−05 0.029400015 0.014375073 MKI67 1.07E−14 0.001551665 0.01475575 MYO1E 6.64E−19 0.000121737 0.016029436 TOP2A 0.000178288 0.002190691 0.016288455 NDFIP2 5.27E−07 0.013620929 0.016478506 PDE7B 8.43E−09 0.000551192 0.023019759 CDC6 3.27E−05 0.005879161 0.028458522 LOC1019289 6.32E−07 0.033973184 0.028458522 TNFRSF18 0.014728033 0.01044711 0.031578469 WIPF3 3.17E−12 2.29E−11 0.033969958 CKAP2L 0.000147773 0.015040042 0.036776193 AMZ1 1.69E−08 0.019661184 0.036835065 ITGA2 3.62E−14 0.004195126 0.04036681 MZB1 5.14E−08 0.001740835 0.041547254 VCAM1 1.11E−06 0.001304561 0.041909455 TIAM2 0.003517055 0.006107399 0.042203047 SLC27A2 4.94E−06 0.030514706 0.042334312 UHRF1 2.43E−07 3.57E−05 0.046651354 RIC1 0.04745656 0.030514706 0.048428633 EMC9 0.01302164 3.57E−05 0.048558511 CDK1 0.003453732 0.044785941 0.049332993 PLAC8 9.79E−13 0.037207354 9.38E−14 FCGR3A 3.65E−17 2.20E−05 8.59E−11 KLRF1 4.80E−41 0.026882065 1.41E−05 DHRS3 4.10E−10 0.007520596 3.52E−05 FCGR3B 2.23E−14 6.81E−06 4.77E−05 CXCL16 9.77E−19 5.90E−08 0.000968842 LYZ 3.71E−22 6.99E−16 0.0023407 SVIL 3.54E−15 0.001007621 0.002803752 PXN 5.35E−11 8.53E−05 0.006403473 CRIP2 3.63E−08 1.18E−12 0.007502923 CCL18 3.93E−06 0.005101201 0.023344239 C1QC 3.49E−11 0.000129199 0.025776769 C1orf162 5.83E−08 0.000223456 0.029224437 PLAUR 1.13E−16 0.001290023 0.033969958 TYROBP 2.77E−05 0.000915616 0.046142286 MeanTPM- MeanTPM_ MeanTPM_ Tumor-TRM NIL_CD103neg NIL_CD103pos GSG2 10.79137868 0.161439895 8.00016295 MYO7A 44.23771526 0.038636476 13.82020979 LAYN 52.53625789 1.268013443 5.637401785 KRT86 90.02109042 0.77714719 21.05319575 STMN1 171.0376579 79.23568095 93.6313875 ENTPD1 51.02960526 4.813320905 8.00948545 KLRC2 170.3632789 27.43117567 71.7629798 TOX 111.2167158 40.17317381 48.47420821 TNS3 18.24837105 2.614606619 1.99195922 SRGAP3 21.03388737 0.327200095 6.54159999 CLNK 48.79801947 0.025489571 37.81930265 AFAP1L2 48.02176947 0.917768462 5.888400985 AKAP5 12.78739474 0.936769381 2.15521775 CXCL13 1830.654842 11.14535171 33.24851965 HAVCR2 334.6920737 43.65628381 59.708044 KIR2DL4 90.73159421 3.772644619 20.33869545 CHRM3-AS2 45.31885842 12.42002929 16.6765635 TNFRSF9 75.16161053 13.75290395 7.85170705 RRM2 51.55875105 5.512094643 12.54809355 CD109 4.155530368 1.260725819 2.672376735 DBH-AS1 9.190445 0 2.82368685 SIRPG 477.9031579 133.5109523 86.54938765 CCL3 879.4891053 379.4398524 308.7706215 KIF2C 15.13782305 0.463829524 1.61216448 CTLA4 243.8928526 19.54678733 68.7486734 IFITM10 11.72409947 2.392565238 3.45431005 GEM 25.92595789 2.124119605 1.49090195 RAD51AP1 17.47792795 6.90577151 4.517111 KIFC1 2.437878232 1.01071879 1.177614 NUSAP1 89.48275263 20.39847528 33.052021 AURKA 26.20345458 5.976808429 25.7213712 VDR 10.44611358 5.016252971 2.20061547 KIAA0101 57.27524105 6.675146857 25.12704715 ZC3H12C 1.357966053 0.011761848 0.03016842 ZBED2 44.29435947 0.845607048 3.18071175 LILRP2 8.555959895 0.179393571 2.0487929 FANCI 18.30659889 7.114525952 7.359242145 TNFSF4 41.49277777 2.741550938 6.68748513 ASB2 43.76039474 4.745831905 9.542647915 CAMK1 54.144063 7.5266972 21.17401165 ANKS1B 2.183310789 0.070079129 0.13357678 SUOX 16.12955742 4.120592919 2.128606905 KCNK5 20.51417421 1.4114012 5.010913685 KIF14 3.265626621 1.26698571 0.191578765 SYNGR3 42.42283158 0.91940919 13.9666646 C1orf106 2.195005284 1.078111543 0.415545935 CCNA2 30.29312263 5.956798548 3.153623335 CCRL2 41.17150789 17.92357785 15.53568475 GINS1 9.327755947 1.102519233 1.842860195 TK1 62.27577368 26.89102266 37.6692809 BIRC5 21.82443158 9.183895781 17.58911704 CASC5 6.408608889 0.954572352 1.822563205 INPP5F 14.65299721 0.683434986 6.52550963 MCM2 34.84616211 9.475945462 13.86870412 HJURP 10.34542453 1.195678052 2.683584425 RDH10 13.67609332 6.140745857 4.51163387 FUT8 39.12060211 14.37280957 18.70667616 MKI67 9.95709 0.396551648 2.29143949 MYO1E 15.26436368 1.932618633 2.655199115 TOP2A 32.63963384 6.18349561 16.73250851 NDFIP2 91.40315263 16.69332039 39.17467275 PDE7B 4.094892895 0.369573876 0.69439915 CDC6 6.325555684 0.878381595 1.80925055 LOC1019289 13.66175105 1.286569143 5.42855735 TNFRSF18 73.40014211 16.56000143 18.60834935 WIPF3 18.05181664 1.792744448 1.021894 CKAP2L 7.193064868 0.582763748 2.08007515 AMZ1 4.697318947 0.071634724 1.16409654 ITGA2 5.027070905 0.309187614 1.389590445 MZB1 56.75498526 9.430024524 16.1069718 VCAM1 77.7627 12.47772077 20.12920756 TIAM2 3.874846842 0.695292919 1.160521885 SLC27A2 74.47079316 7.761453905 26.6987376 UHRF1 9.493753579 2.803213095 2.78529736 RIC1 9.940590942 4.073464 2.943654765 EMC9 55.71777368 30.20844119 20.39791095 CDK1 34.75512211 7.678148262 40.57957299 PLAC8 10.19808989 114.6815 26.4785965 FCGR3A 14.86318363 357.0966381 74.9510268 KLRF1 3.035728737 193.5823511 14.4933614 DHRS3 53.74266979 222.4045952 143.5919088 FCGR3B 1.389735158 28.66079204 24.70543001 CXCL16 13.30089653 175.1718914 85.036884 LYZ 197.5742774 2101.34619 1035.50759 SVIL 0.869931489 9.580544286 4.22403719 PXN 19.01642169 126.245899 71.63926645 CRIP2 1.230886568 22.21384857 23.8480978 CCL18 32.81444384 265.5379826 200.3426554 C1QC 59.72089795 365.8164711 164.5710445 C1orf162 55.57553158 321.1258619 216.6321875 PLAUR 20.07343137 271.6738353 50.50656925 TYROBP 111.0874333 449.8064571 378.0819685 MeanTPM_ TIL_CD103neg Min.log2FC padj_Min GSG2 0.43646014 1.727403278 0.017682536 MYO7A 2.571285816 2.259998486 0.000734598 LAYN 1.848024708 3.621535741 1.47E−13 KRT86 3.71512388 2.036854575 0.020077613 STMN1 85.79118 1.315548427 0.016592582 ENTPD1 9.068538 2.51222904 7.94E−12 KLRC2 42.90052344 1.140028446 0.040436251 TOX 52.981492 1.04690617 1.20E−09 TNS3 1.578477292 2.871610018 5.17E−09 SRGAP3 1.467354292 2.092844412 0.004201487 CLNK 3.97429732 1.421442605 0.034357992 AFAP1L2 10.19192002 2.730768148 7.35E−09 AKAP5 3.498434 2.119777167 2.15E−08 CXCL13 200.4329206 2.786215532 2.15E−08 HAVCR2 71.16694344 2.115630958 2.22E−08 KIR2DL4 6.891746048 2.424076288 0.003617679 CHRM3-AS2 2.12001284 2.363451642 0.003597258 TNFRSF9 17.28012864 2.018972638 1.35E−07 RRM2 24.39809328 2.032567257 8.03E−07 CD109 1.058001288 1.712328303 0.00737706 DBH-AS1 1.18816808 2.112220802 0.035666244 SIRPG 185.4196008 1.173047291 2.24E−06 CCL3 448.815904 1.308047955 3.37E−06 KIF2C 2.716626244 2.340084386 4.65E−06 CTLA4 50.97031992 2.049857964 3.76E−05 IFITM10 1.272724276 2.259689528 5.64E−05 GEM 2.765744876 2.209029285 0.000114883 RAD51AP1 2.22739396 2.090366569 0.015324702 KIFC1 1.139757476 2.048040416 0.000116485 NUSAP1 50.15568704 1.471883402 0.000146458 AURKA 17.55234268 1.660078116 0.00018727 VDR 3.135480952 1.916130686 0.000204746 KIAA0101 34.92798628 1.705284926 0.000467234 ZC3H12C 0.060009268 2.032896561 0.000578224 ZBED2 8.41407728 1.958522644 0.000775184 LILRP2 0.24319804 1.929179014 0.00115957 FANCI 5.771166696 1.767956945 0.001462592 TNFSF4 8.7365456 1.864855811 0.001607152 ASB2 13.9961398 1.517671152 0.00161221 CAMK1 12.76232984 1.691816551 0.00313345 ANKS1B 0.373814604 1.817118618 0.003144996 SUOX 2.148762428 1.791011507 0.003317961 KCNK5 3.473615356 1.782686156 0.003491967 KIF14 0.70509062 1.61077793 0.003902254 SYNGR3 10.13336744 1.663443022 0.005290731 C1orf106 0.429621136 1.654127927 0.005493609 CCNA2 16.76836842 1.63474981 0.006225719 CCRL2 18.98001112 1.61789572 0.006388745 GINS1 2.35536462 1.561048491 0.006694195 TK1 34.81022376 1.556990809 0.007377908 BIRC5 17.88640092 1.52789537 0.007475706 CASC5 0.7152126 1.683852608 0.008379571 INPP5F 4.56110998 1.557089828 0.010295127 MCM2 12.7239374 1.417554569 0.013573646 HJURP 3.0432999 1.512082809 0.013662027 RDH10 4.7784252 1.392983915 0.014000421 FUT8 16.45712298 1.270544325 0.014375073 MKI67 3.740788684 1.482800913 0.01475575 MYO1E 3.620583316 1.582425887 0.016029436 TOP2A 14.67442033 1.352846874 0.016288455 NDFIP2 40.7288212 1.116525313 0.016478506 PDE7B 1.530637224 1.258502263 0.023019759 CDC6 4.7555254 1.282112236 0.028458522 LOC1019289 5.24736404 1.400426941 0.028458522 TNFRSF18 20.86098484 1.436215773 0.031578469 WIPF3 4.911925736 1.394904839 0.033969958 CKAP2L 3.42151756 1.321005047 0.036776193 AMZ1 0.757580336 1.401084929 0.036835065 ITGA2 3.19618754 1.216143898 0.04036681 MZB1 24.83474744 1.309894553 0.041547254 VCAM1 44.44741996 1.142380045 0.041909455 TIAM2 0.52895268 1.421865316 0.042203047 SLC27A2 31.71734129 1.267279008 0.042334312 UHRF1 4.08198472 1.171771134 0.046651354 RIC1 4.893008408 1.123345605 0.048428633 EMC9 29.22870432 1.162931851 0.01302164 CDK1 15.2390608 1.312177938 0.049332993 PLAC8 55.3151272 −1.464685708 0.037207354 FCGR3A 119.760642 −2.930611162 8.59E−11 KLRF1 69.15205336 −2.393123248 1.41E−05 DHRS3 150.522892 −1.538676923 3.52E−05 FCGR3B 15.66373078 −2.089580696 4.77E−05 CXCL16 33.54755604 −1.465177681 0.000968842 LYZ 533.8098288 −1.206783578 0.0023407 SVIL 6.2219778 −1.730549769 0.002803752 PXN 60.2272196 −1.349346038 0.006403473 CRIP2 10.84884325 −1.682549856 0.007502923 CCL18 120.7602916 −1.43447936 0.023344239 C1QC 122.636079 −1.289800192 0.025776769 C1orf162 149.498455 −1.321986663 0.029224437 PLAUR 88.264021 −1.41499774 0.033969958 TYROBP 286.6825624 −1.130633801 0.046142286

TABLE 6 Mapping metics obtained from MIGIC analysis Estimate SAMPLE Name Number Sample Class Marker TYPE 1 12-TL647-TIL-CD8+_CD103+ 12 TL647 TIL CD8+_CD103+ paired 2 13-TL647-TIL-CD8+_CD103− 13 TL647 TIL CD8+_CD103− paired 3 139-TL706-TIL-CD8+_CD103+ 139 TL706 TIL CD8+_CD103+ paired 4 140-TL706-TIL-CD8+_CD103− 140 TL706 TIL CD8+_CD103− paired 5 151-TL722-TIL-CD8+_CD103+ 151 TL722 TIL CD8+_CD103+ paired 6 152-TL722-TIL-CD8+_CD103− 152 TL722 TIL CD8+_CD103− paired 7 157-TL704-TIL-CD8+_CD103+ 157 TL704 TIL CD8+_CD103+ paired 8 158-TL704-TIL-CD8+_CD103− 158 TL704 TIL CD8+_CD103− paired 9 172-TL720-TIL-CD8+_CD103+ 172 TL720 TIL CD8+_CD103+ paired 10 173-TL720-TIL-CD8+_CD103− 173 TL720 TIL CD8+_CD103− paired 11 18-TL615-TIL-CD8+_CD103+ 18 TL615 TIL CD8+_CD103+ paired 12 19-TL615-TIL-CD8+_CD103− 19 TL615 TIL CD8+_CD103− paired 13 55-TL661-TIL-CD8+_CD103+ 55 TL661 TIL CD8+_CD103+ paired 14 56-TL661-TIL-CD8+_CD103− 56 TL661 TIL CD8+_CD103− paired 15 63-TL663-TIL-CD8+_CD103+ 63 TL663 TIL CD8+_CD103+ paired 16 64-TL663-TIL-CD8+_CD103− 64 TL663 TIL CD8+_CD103− paired 17 90-TL101-TIL-CD8+_CD103+ 90 TL101 TIL CD8+_CD103+ paired 18 91-TL101-TIL-CD8+_CD103− 91 TL101 TIL CD8+_CD103− paired 19 95-TL684-TIL-CD8+_CD103+ 95 TL684 TIL CD8+_CD103+ paired 20 96-TL684-TIL-CD8+_CD103− 96 TL684 TIL CD8+_CD103− paired Estimate Estimate Estimate Estimate Estimate TOTAL TOTAL OVERSEQ COLLISION UMI QUAL Name READS MIGS THRESHOLD THRESHOLD THRESHOLD 1 12-TL647-TIL-CD8+_CD103+ 256397 3483 16 16 15 2 13-TL647-TIL-CD8+_CD103− 105892 1787 16 16 15 3 139-TL706-TIL-CD8+_CD103+ 157923 3661 11 11 15 4 140-TL706-TIL-CD8+_CD103− 414797 4264 23 23 15 5 151-TL722-TIL-CD8+_CD103+ 144130 3141 11 11 15 6 152-TL722-TIL-CD8+_CD103− 193457 1743 32 32 15 7 157-TL704-TIL-CD8+_CD103+ 242088 3713 11 11 15 8 158-TL704-TIL-CD8+_CD103− 228663 3040 16 16 15 9 172-TL720-TIL-CD8+_CD103+ 185525 1773 32 32 15 10 173-TL720-TIL-CD8+_CD103− 158541 1973 16 16 15 11 18-TL615-TIL-CD8+_CD103+ 230107 4147 11 11 15 12 19-TL615-TIL-CD8+_CD103− 294826 3764 16 16 15 13 55-TL661-TIL-CD8+_CD103+ 179352 2788 16 16 15 14 56-TL661-TIL-CD8+_CD103− 62968 1385 11 11 15 15 63-TL663-TIL-CD8+_CD103+ 262129 4085 16 16 15 16 64-TL663-TIL-CD8+_CD103− 261288 3438 23 23 15 17 90-TL101-TIL-CD8+_CD103+ 125051 1037 32 32 15 18 91-TL101-TIL-CD8+_CD103− 65514 2602 6 6 15 19 95-TL684-TIL-CD8+_CD103+ 290295 2234 32 32 15 20 96-TL684-TIL-CD8+_CD103− 167628 1297 32 32 15 Assemble Assemble Assemble Assemble Estimate MIG COUNT MIGS GOOD MIGS GOOD MIGS GOOD Name UMI LEN THRESHOLD FASTQ1 FASTQ2 TOTAL 1 12-TL647-TIL-CD8+_CD103+ 12 16 881 886 874 2 13-TL647-TIL-CD8+_CD103− 12 16 317 317 313 3 139-TL706-TIL-CD8+_CD103+ 12 11 1548 1555 1540 4 140-TL706-TIL-CD8+_CD103− 12 23 743 745 741 5 151-TL722-TIL-CD8+_CD103+ 12 11 1346 1344 1329 6 152-TL722-TIL-CD8+_CD103− 12 32 152 155 150 7 157-TL704-TIL-CD8+_CD103+ 12 11 1489 1491 1483 8 158-TL704-TIL-CD8+_CD103− 12 16 728 727 716 9 172-TL720-TIL-CD8+_CD103+ 12 32 219 215 214 10 173-TL720-TIL-CD8+_CD103− 12 16 524 526 523 11 18-TL615-TIL-CD8+_CD103+ 12 11 1488 1470 1458 12 19-TL615-TIL-CD8+_CD103− 12 16 906 905 895 13 55-TL661-TIL-CD8+_CD103+ 12 16 578 581 573 14 56-TL661-TIL-CD8+_CD103− 12 11 310 310 308 15 63-TL663-TIL-CD8+_CD103+ 12 16 720 724 717 16 64-TL663-TIL-CD8+_CD103− 12 23 511 518 510 17 90-TL101-TIL-CD8+_CD103+ 12 32 187 188 186 18 91-TL101-TIL-CD8+_CD103− 12 6 1704 1705 1682 19 95-TL684-TIL-CD8+_CD103+ 12 32 276 275 275 20 96-TL684-TIL-CD8+_CD103− 12 32 180 180 180 Assemble Assemble Assemble Assemble READS READS READS Assemble MIGS GOOD GOOD GOOD READS Name TOTAL FASTQ1 FASTQ2 TOTAL TOTAL 1 12-TL647-TIL-CD8+_CD103+ 3483 215978 224093 235916 242518 2 13-TL647-TIL-CD8+_CD103− 1787 90486 93526 94556 99609 3 139-TL706-TIL-CD8+_CD103+ 3661 137863 140542 144314 148820 4 140-TL706-TIL-CD8+_CD103− 4264 361751 373821 382965 389088 5 151-TL722-TIL-CD8+_CD103+ 3141 125468 129630 131603 136224 6 152-TL722-TIL-CD8+_CD103− 1743 170625 174451 176010 181048 7 157-TL704-TIL-CD8+_CD103+ 3713 213284 216903 224884 229289 8 158-TL704-TIL-CD8+_CD103− 3040 196480 205688 209293 215321 9 172-TL720-TIL-CD8+_CD103+ 1773 163398 169622 171133 175191 10 173-TL720-TIL-CD8+_CD103− 1973 141013 145563 146520 148966 11 18-TL615-TIL-CD8+_CD103+ 4147 200782 205033 210574 216662 12 19-TL615-TIL-CD8+_CD103− 3764 256428 265348 269313 277718 13 55-TL661-TIL-CD8+_CD103+ 2788 154888 158301 161188 167841 14 56-TL661-TIL-CD8+_CD103− 1385 53932 55422 55888 58920 15 63-TL663-TIL-CD8+_CD103+ 4085 225440 234365 236938 246198 16 64-TL663-TIL-CD8+_CD103− 3438 221547 233768 235752 245422 17 90-TL101-TIL-CD8+_CD103+ 1037 108119 114592 115402 117838 18 91-TL101-TIL-CD8+_CD103− 2602 56311 57744 59538 61447 19 95-TL684-TIL-CD8+_CD103+ 2234 255442 265674 268247 271670 20 96-TL684-TIL-CD8+_CD103− 1297 145259 154277 156186 158039 Assemble READS DROPPED CDRBlast CDRBlast CDRBlast CDRBlast WITHIN DATA EVENTS EVENTS EVENTS Name MIG TYPE GOOD MAPPED TOTAL 1 12-TL647-TIL-CD8+_CD103+ 32002 asm 699 741 1748 2 13-TL647-TIL-CD8+_CD103− 6573 asm 259 263 626 3 139-TL706-TIL-CD8+_CD103+ 10968 asm 1068 1183 3080 4 140-TL706-TIL-CD8+_CD103− 30862 asm 494 519 1482 5 151-TL722-TIL-CD8+_CD103+ 9686 asm 929 1054 2658 6 152-TL722-TIL-CD8+_CD103− 6825 asm 90 95 300 7 157-TL704-TIL-CD8+_CD103+ 20961 asm 1054 1161 2966 8 158-TL704-TIL-CD8+_CD103− 17696 asm 592 652 1432 9 172-TL720-TIL-CD8+_CD103+ 8793 asm 150 164 428 10 173-TL720-TIL-CD8+_CD103− 6904 asm 407 429 1046 11 18-TL615-TIL-CD8+_CD103+ 16414 asm 1139 1277 2916 12 19-TL615-TIL-CD8+_CD103− 19363 asm 731 780 1790 13 55-TL661-TIL-CD8+_CD103+ 10405 asm 412 422 1146 14 56-TL661-TIL-CD8+_CD103− 2553 asm 236 244 616 15 63-TL663-TIL-CD8+_CD103+ 15508 asm 572 582 1434 16 64-TL663-TIL-CD8+_CD103− 17675 asm 374 377 1020 17 90-TL101-TIL-CD8+_CD103+ 9472 asm 123 123 372 18 91-TL101-TIL-CD8+_CD103− 5835 asm 1167 1455 3364 19 95-TL684-TIL-CD8+_CD103+ 14558 asm 202 203 550 20 96-TL684-TIL-CD8+_CD103− 10653 asm 136 136 360 CDRBlast CDRBlast CDRBlast Number Number READS READS READS TCR Clonotypes Name GOOD MAPPED TOTAL molecules Found 1 12-TL647-TIL-CD8+_CD103+ 175402 177879 439744 654 99 2 13-TL647-TIL-CD8+_CD103− 77762 77873 183383 237 67 3 139-TL706-TIL-CD8+_CD103+ 87825 93692 278009 974 157 4 140-TL706-TIL-CD8+_CD103− 212346 231002 734848 472 175 5 151-TL722-TIL-CD8+_CD103+ 83161 92665 254326 878 117 6 152-TL722-TIL-CD8+_CD103− 104254 104440 344846 85 60 7 157-TL704-TIL-CD8+_CD103+ 119636 128407 428990 998 68 8 158-TL704-TIL-CD8+_CD103− 154956 162679 401324 558 224 9 172-TL720-TIL-CD8+_CD103+ 119858 120386 332822 143 85 10 173-TL720-TIL-CD8+_CD103− 114808 115925 286238 380 146 11 18-TL615-TIL-CD8+_CD103+ 160660 168339 405084 1053 137 12 19-TL615-TIL-CD8+_CD103− 226581 228568 520271 674 192 13 55-TL661-TIL-CD8+_CD103+ 122895 124004 312583 387 108 14 56-TL661-TIL-CD8+_CD103− 43713 43813 109221 216 111 15 63-TL663-TIL-CD8+_CD103+ 178541 182139 458777 550 163 16 64-TL663-TIL-CD8+_CD103− 166911 168724 453958 350 171 17 90-TL101-TIL-CD8+_CD103+ 53875 53875 222323 118 47 18 91-TL101-TIL-CD8+_CD103− 40252 46213 113574 1047 653 19 95-TL684-TIL-CD8+_CD103+ 202918 202966 520997 190 103 20 96-TL684-TIL-CD8+_CD103− 104038 104038 299536 129 92 Sample ID Sample Class Marker Filter 12-TL647-TIL-CD8+_CD103+ TL647 TIL CD8+_CD103+ conv:MiGec 13-TL647-TIL-CD8+_CD103− TL647 TIL CD8+_CD103− conv:MiGec 139-TL706-TIL-CD8+_CD103+ TL706 TIL CD8+_CD103+ conv:MiGec 140-TL706-TIL-CD8+_CD103− TL706 TIL CD8+_CD103− conv:MiGec 151-TL722-TIL-CD8+_CD103+ TL722 TIL CD8+_CD103+ conv:MiGec 152-TL722-TIL-CD8+_CD103− TL722 TIL CD8+_CD103− conv:MiGec 157-TL704-TIL-CD8+_CD103+ TL704 TIL CD8+_CD103+ conv:MiGec 158-TL704-TIL-CD8+_CD103− TL704 TIL CD8+_CD103− conv:MiGec 172-TL720-TIL-CD8+_CD103+ TL720 TIL CD8+_CD103+ conv:MiGec 173-TL720-TIL-CD8+_CD103− TL720 TIL CD8+_CD103− conv:MiGec 18-TL615-TIL-CD8+_CD103+ TL615 TIL CD8+_CD103+ conv:MiGec 19-TL615-TIL-CD8+_CD103− TL615 TIL CD8+_CD103− conv:MiGec 55-TL661-TIL-CD8+_CD103+ TL661 TIL CD8+_CD103+ conv:MiGec 56-TL661-TIL-CD8+_CD103− TL661 TIL CD8+_CD103− conv:MiGec 63-TL663-TIL-CD8+_CD103+ TL663 TIL CD8+_CD103+ conv:MiGec 64-TL663-TIL-CD8+_CD103− TL663 TIL CD8+_CD103− conv:MiGec 90-TL101-TIL-CD8+_CD103+ TL101 TIL CD8+_CD103+ conv:MiGec 91-TL101-TIL-CD8+_CD103− TL101 TIL CD8+_CD103− conv:MiGec 95-TL684-TIL-CD8+_CD103+ TL684 TIL CD8+_CD103+ conv:MiGec 96-TL684-TIL-CD8+_CD103− TL684 TIL CD8+_CD103− conv:MiGec Extrapolate Chao1 Sample ID Read Diversity reads mean 12-TL647-TIL-CD8+_CD103+ 654 99 1053 235 13-TL647-TIL-CD8+_CD103− 237 67 1053 166 139-TL706-TIL-CD8+_CD103+ 974 157 1053 265 140-TL706-TIL-CD8+_CD103− 472 175 1053 451 151-TL722-TIL-CD8+_CD103+ 878 117 1053 203 152-TL722-TIL-CD8+ CD103− 85 60 1053 163 157-TL704-TIL-CD8+_CD103+ 998 68 1053 103 158-TL704-TIL-CD8+_CD103− 558 224 1053 750 172-TL720-TIL-CD8+_CD103+ 143 85 1053 269 173-TL720-TIL-CD8+_CD103− 380 146 1053 344 18-TL615-TIL-CD8+_CD103+ 1053 137 1053 252 19-TL615-TIL-CD8+_CD103− 674 192 1053 446 55-TL661-TIL-CD8+_CD103+ 387 108 1053 165 56-TL661-TIL-CD8+_CD103− 216 111 1053 274 63-TL663-TIL-CD8+_CD103+ 550 163 1053 326 64-TL663-TIL-CD8+_CD103− 350 171 1053 473 90-TL101-TIL-CD8+_CD103+ 118 47 1053 152 91-TL101-TIL-CD8+_CD103− 1047 653 1053 1848 95-TL684-TIL-CD8+_CD103+ 190 103 1053 259 96-TL684-TIL-CD8+_CD103− 129 92 1053 262 ObservedDiversity ChaoE ChaoE EfronThisted EfronThisted Sample ID mean mean std mean std 12-TL647-TIL-CD8+_CD103+ 99 131 8 207 15 13-TL647-TIL-CD8+_CD103− 67 145 23 112 6 139-TL706-TIL-CD8+_CD103+ 157 163 8 295 19 140-TL706-TIL-CD8+_CD103− 175 287 16 387 22 151-TL722-TIL-CD8+_CD103+ 117 129 7 224 16 152-TL722-TIL-CD8+_CD103− 60 162 41 106 6 157-TL704-TIL-CD8+_CD103+ 68 69 4 95 5 158-TL704-TIL-CD8+_CD103− 224 352 16 654 41 172-TL720-TIL-CD8+_CD103+ 85 251 48 152 8 173-TL720-TIL-CD8+_CD103− 146 261 19 319 20 18-TL615-TIL-CD8+_CD103+ 137 137 7 268 18 19-TL615-TIL-CD8+_CD103− 192 252 11 408 22 55-TL661-TIL-CD8+_CD103+ 108 154 12 194 15 56-TL661-TIL-CD8+_CD103− 111 246 32 245 17 63-TL663-TIL-CD8+_CD103+ 163 232 12 331 20 64-TL663-TIL-CD8+_CD103− 171 338 24 390 22 90-TL101-TIL-CD8+_CD103+ 47 145 41 83 6 91-TL101-TIL-CD8+_CD103− 653 655 20 2075 108 95-TL684-TIL-CD8+_CD103+ 103 238 35 227 17 96-TL684-TIL-CD8+_CD103− 92 254 48 164 8 normalized Chao1 d50Index shannonWienerIndex ShannonWienerIndex inverseSimpsonIndex Sample ID std mean mean mean mean 12-TL647-TIL-CD8+_CD103+ 52 0.95959596 20.53006238 0.6576303 10.35732274 13-TL647-TIL-CD8+_CD103− 43 0.955223881 17.98794136 0.6872563 5.595079191 139-TL706-TIL-CD8+_CD103+ 31 0.961783439 36.38352009 0.710827 16.06999356 140-TL706-TIL-CD8+_CD103− 76 0.92 68.48994371 0.8183663 19.89142857 151-TL722-TIL-CD8+_CD103+ 29 0.965811966 24.32970049 0.6702187 10.55629502 152-TL722-TIL-CD8+_CD103− 44 0.9 51.00535901 0.9603321 40.81920904 157-TL704-TIL-CD8+_CD103+ 18 0.911764706 21.09133591 0.7225635 11.21575605 158-TL704-TIL-CD8+_CD103− 132 0.9375 93.37867751 0.8383148 33.0675446 172-TL720-TIL-CD8+_CD103+ 69 0.929411765 53.57509948 0.8961055 25.72201258 173-TL720-TIL-CD8+_CD103− 58 0.904109589 70.32517191 0.8534241 31.58355206 18-TL615-TIL-CD8+_CD103+ 36 0.97080292 24.05847811 0.646443 9.060895786 19-TL615-TIL-CD8+_CD103− 67 0.942708333 68.829179 0.8048752 29.23645257 55-TL661-TIL-CD8+_CD103+ 20 0.944444444 37.173325 0.7722106 13.21296868 56-TL661-TIL-CD8+_CD103− 55 0.90990991 77.24672227 0.923023 50.06008584 63-TL663-TIL-CD8+_CD103+ 46 0.895705521 64.85979256 0.8190877 24.16520211 64-TL663-TIL-CD8+_CD103− 83 0.941520468 98.46364678 0.8926464 43.01264045 90-TL101-TIL-CD8+_CD103+ 53 0.936170213 19.01294967 0.764937 8.307875895 91-TL101-TIL-CD8+_CD103− 165 0.977029096 485.3991287 0.9542387 299.920383 95-TL684-TIL-CD8+_CD103+ 55 0.912621359 77.11035818 0.9375387 55.53846154 96-TL684-TIL-CD8+_CD103− 60 0.923913043 76.75366646 0.9599301 57.9825784 Name Number Sample Class Marker 12-TL647-TIL-CD8+_CD103+ 12 TL647 TIL CD8+_CD103+ 13-TL647-TIL-CD8+_CD103− 13 TL647 TIL CD8+_CD103− 139-TL706-TIL-CD8+_CD103+ 139 TL706 TIL CD8+_CD103+ 140-TL706-TIL-CD8+_CD103− 140 TL706 TIL CD8+_CD103− 151-TL722-TIL-CD8+_CD103+ 151 TL722 TIL CD8+_CD103+ 152-TL722-TIL-CD8+_CD103− 152 TL722 TIL CD8+_CD103− 157-TL704-TIL-CD8+_CD103+ 157 TL704 TIL CD8+_CD103+ 158-TL704-TIL-CD8+_CD103− 158 TL704 TIL CD8+_CD103− 172-TL720-TIL-CD8+_CD103+ 172 TL720 TIL CD8+_CD103+ 173-TL720-TIL-CD8+_CD103− 173 TL720 TIL CD8+_CD103− 18-TL615-TIL-CD8+_CD103+ 18 TL615 TIL CD8+_CD103+ 19-TL615-TIL-CD8+_CD103− 19 TL615 TIL CD8+_CD103− 55-TL661-TIL-CD8+_CD103+ 55 TL661 TIL CD8+_CD103+ 56-TL661-TIL-CD8+_CD103− 56 TL661 TIL CD8+_CD103− 63-TL663-TIL-CD8+_CD103+ 63 TL663 TIL CD8+_CD103+ 64-TL663-TIL-CD8+_CD103− 64 TL663 TIL CD8+_CD103− 90-TL101-TIL-CD8+_CD103+ 90 TL101 TIL CD8+_CD103+ 91-TL101-TIL-CD8+_CD103− 91 TL101 TIL CD8+_CD103− 95-TL684-TIL-CD8+_CD103+ 95 TL684 TIL CD8+_CD103+ 96-TL684-TIL-CD8+_CD103− 96 TL684 TIL CD8+_CD103− Percent top Percent sec Percent rem Percent all Percent non Name exp clone exp clones exp clones exp clones exp clones 12-TL647-TIL-CD8+_CD103+ 17 15 55 87 13 13-TL647-TIL-CD8+_CD103− 41 8 25 73 27 139-TL706-TIL-CD8+_CD103+ 16 10 59 85 15 140-TL706-TIL-CD8+_CD103− 19 7 39 65 35 151-TL722-TIL-CD8+_CD103+ 22 15 51 87 13 152-TL722-TIL-CD8+ CD103− 7 6 12 25 75 157-TL704-TIL-CD8+_CD103+ 24 9 63 95 5 158-TL704-TIL-CD8+_CD103− 13 5 43 61 39 172-TL720-TIL-CD8+_CD103+ 15 8 15 38 62 173-TL720-TIL-CD8+_CD103− 11 8 43 62 38 18-TL615-TIL-CD8+_CD103+ 27 11 49 88 12 19-TL615-TIL-CD8+_CD103− 9 9 55 74 26 55-TL661-TIL-CD8+_CD103+ 22 12 39 72 28 56-TL661-TIL-CD8+_CD103− 7 6 38 50 50 63-TL663-TIL-CD8+_CD103+ 16 6 49 72 28 64-TL663-TIL-CD8+_CD103− 11 7 35 52 48 90-TL101-TIL-CD8+_CD103+ 26 20 14 61 39 91-TL101-TIL-CD8+_CD103− 2 2 31 35 65 95-TL684-TIL-CD8+_CD103+ 5 8 35 49 51 96-TL684-TIL-CD8+_CD103− 6 5 12 22 78

TCRβ chain reconstruction in subjects from TCR-seq analysis Number Name Class Marker CDR3 nucleotide sequence 1 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGAAGGAGGGCGACTAGAGGCAGATACGCAGTATTTT 2 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCCTCATCCCTTGGACAGGACAATCAGCCCCAGCATTTT 3 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGGTCAGGGGAGTACATTCAGTACTTC 4 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCAGGGACTAGCTACATTCAGTTCTTC 5 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTAGCTAGCGGGACAGATACGCAGTATTTT 6 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTATCAAGGAGCCAGTCCTCTAAAGCTTTCTTT 7 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCGACAGGGTACTATGGCTACACCTTC 8 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTCCGGACAGGGGGCCACTGAAGCTTTCTTT 9 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGGATAGCAATCAGCCCCAGCATTTT 10 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGAGGGGGCTTATACGAGCAGTACTTC 11 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGACAGGGGGTGATGGCTACACCTTC 12 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCCATAGCGGGGAGCTCCTACAATGAGCAGTTCTTC 13 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATAGGGCAGCAAGAGCAGTTCTTC 14 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGTAGGACAGGCAATGAGCAGTTCTTC 15 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCCAGACTGGTTCCAGGTCTACGAGCAGTACTTC 16 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGGGGACTACATGGACGCAGTATTTT 17 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGAAAGAGGACTCACTGAAGCTTTCTTT 18 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGGAACCAGTAGGACCTTACAATGAGCAGTTCTTC 19 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGGCGGAGGGAGGTTAACGCAGTATTTT 20 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCTCTTGGGGACCCTAGCTCCGGGGAGCTGTTTTTT 21 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACCCCCGATGGGGCGAATCAGTACTTC 22 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCATTACCGGGACAGGGAAACCCTACGAGCAGTACTTC 23 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTCATGCGGCCGGGACAGGGGGCGGTGGGGGATTCA CCCCTCCACTTT 24 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTTCGTGGGCTTGGAGCTTTCTTT 25 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCCGACTAGCGGGGCTCTACGAGCAGTACTTC 26 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGCGGCTAGCGGGCGCCTCCCTTTACAATGAG CAGTTCTTC 27 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCTACAGGCCAAGAGACCCAGTACTTC 28 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGAAGGGGGTCGGGGGCGGGGGGATACGCAGTATTTT 29 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGATGACCTTAAACCTGCCGAGCAGTACTTC 30 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGGGCTTTACTCAGATACGCAGTATTTT 31 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGCCGGGACGTCCCATCAGCCCCAGCATTTT 32 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTACTACTCGACAGGGGGGTGTAAGAAATCAGCCCCAGCATTTT 33 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGAGGGAACTAGCGCGACCTACGAGCAGTACTTC 34 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGTGGTTGGGACAGTAAATTCAATGAGCAGTTCTTC 35 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGTAGCAGGATCGGGGAGCTGTTTTTT 36 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGGGACAGTCTGTGGACACCGGGGAGCTGTTTTTT 37 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCGGAGGCAGGGGGAACTACGAGCAGTACTTC 38 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGCCCCGGACAAAGCTAACTATGGCTACACCTTC 39 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTAGATCCCGGGGTCTATGGCTACACCTTC 40 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGGAGGTGACAGCCACCTCAGATACGCAGTATTTT 41 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCTCTAGCGGGAGATGGCGAGCAGTACTTC 42 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTACTTAAGACAACCTGGAACACTGAAGCTTTCTTT 43 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGTCCCAAGACCGGACTACGAGCAGTACTTC 44 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCGCCGGGACAGGAAAAAAAGACCCAGTACTTC 45 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAAGCAAGGGACGGAAGCTCCTACGAGCAGTACTTC 46 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTAGGAAATAGAGGGGGCACAGATACGCAGTATTTT 47 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGCGGTAGCGGGAGTGGGAGAGACCCAGTACTTC 48 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCAACGGGTTATCCTACGAGCAGTACTTC 49 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGTGGGGACGGTATGAACACTGAAGCTTTCTTT 50 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGAAAACACTCACTACGAGCAGTACTTC 51 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGTGGAGGCTCCCACTGAAGCTTTCTTT 52 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCGCCGGGACAGGGAAAAAAGACCCAGTACTTC 53 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCATCTGGACGGAGGTCTCAATCAGCCCCAGCATTTT 54 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGAGTCAGGGCCAGGGAACATTCAGTACTTC 55 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGTTCGTAGGTTCGGGGAGCTGTTTTTT 56 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCGGGCGGGTGGGGGGAGACCCAGTACTTC 57 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGGGCGGGTATGAAACAGATACGCAGTATTTT 58 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGCAACTATGGCTGGCTCCTACAATGAGCAGTTCTTC 59 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCCTCATCCCTTGGACAGGACAATCAGCCCCAGCATTTT 60 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATTAACAGGGGGATGAACACTGAAGCTTTCTTT 61 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGGTCAGGGGAGTACATTCAGTACTTC 62 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGATGCGACAGGGATCTACGAGCAGTACTTC 63 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGATGCTAGCGGGACCACAGATACGCAGTATTTT 64 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCTCCGGGACTAGGTACAGATACGCAGTATTTT 65 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGACAGCGGGAGACTGAACACCGGGGAGCTGTTTTTT 66 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAAACCGGGACAGGGGCGCACATGGCTACACCTTC 67 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGAAGGCAGGGAGGGGGAGACCCAGTACTTC 68 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCTCCTGGAGGCGGGTCAGCCCCAGCATTTT 69 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTACTAGCGGGAGGGTTATACAATGAGCAGTTCTTC 70 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACCGGACTAGCGGACTCAATGAGCAGTTCTTC 71 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTTTCGACACGAACTGGGGCCAACGTCCTGACTTTC 72 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGCCGGCGGACACCACTCCTACGAGCAGTACTTC 73 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCAACGGGGGGTCGGGACGAGCAGTACTTC 74 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGCTAGCGGGGGGTCCACAGATACGCAGTATTTT 75 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCAGGAATAGACAACTATGGCTACACCTTC 76 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTGGGGAGTAACTACAATGAGCAGTTCTTC 77 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTATCCAGAAGCTTTCTTT 78 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACAGGGACAGGGGGGCAGATACGCAGTATTTT 79 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGAACTTATGGGGACATGAACACTGAAGCTTTCTTT 80 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGGGACAGGGTGGTAATTCACCCCTCCACTTT 81 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAAGGAGGGGGGGCACAGATACGCAGTATTTT 82 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTATTTCCCGGGGAGCTGTTTTTT 83 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGAGTCGTACAATGAGCAGTTCTTC 84 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCCGGACAGAACACCGGGGAGCTGTTTTTT 85 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGAGGAGGACAGGGTGGACGAGCAGTACTTC 86 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGAGTGGACAGTGAACGGGGAGCTGTTTTTT 87 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGGGTGGTGGACAGACTATGGCTACACCTTC 88 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCTAGCAGCTTGTGGGGGAGGCCTTCCGATGAGCAGTTCTTC 89 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTATCAAATTCGGGCACTGAAGCTTTCTTT 90 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTGGGCCCGGACTGACGAGCAGTACTTC 91 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAATCTACCCAGGGGTATTCACCCCTCCACTTT 92 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCGGGGGGGGGCACTGAAGCTTTCTTT 93 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGACGGGGGGTACACTGAAGCTTTCTTT 94 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGATCCCCTAGGCCCCTACTCTGGGGCC AACGTCCTGACTTTC 95 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTCGCCCCGAATAACTATGGCTACACCTTC 96 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCCTAGCGGGAGGGCCAGGCGAGCAGTACTTC 97 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCGGACAGGGAGGAAATTCACCCCTCCACTTT 98 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTGCCGGGACCACAAAAGAGGACGAGCAGTACTTC 99 12-TL647-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGCCGGCGGCGCGGGGGTGGAGGAAAAACTGTTTTTT 100 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGAGTCAGGGCCAGGGAACATTCAGTACTTC 101 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTAGACTAGCCCAAGAGACCCAGTACTTC 102 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGAGAGCGGCGGCCCTTACAATGAGCAGTTCTTC 103 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGCGGCCTAGCGGGAGACGACGAGCAGTACTTC 104 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAGTCGTGGGGAGTCACTATGGCTACACCTTC 105 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATGGCAGGGGTCTAATGAAAAACTGTTTTTT 106 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTATCGGAGGGTAATGAGCAGTTCTTC 107 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCAACAATGAGCAGTTCTTC 108 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTTCAGGGGGCCGGACAGATACGCAGTATTTT 109 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTACCCCGGCTTACTTGAACACCGGGGAGCTGTTTTTT 110 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACCAAACCGGGACAGGGGTCTATGGCTACACCTTC 111 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGGTCAAGGGGGGGCTTGGGGCTACACCTTC 112 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCGGGGCAGCTCTCGACCMGAACACTGAAGCTTTCTTT 113 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAACGGGGGGTCGGGACGAGCAGTACTTC 114 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTTACGGGCAGGGAGCCCCTCAATGGAGACCCAGTACTTC 115 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTTCCGGGACAGGGGTATACAATGAGCAGTTCTTC 116 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCAAGGGGGCGCCCTAGGCTACACCTTC 117 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCAACCTGGAGGGGACGGGGAGACTAGCCAAAAACATT CAGTACTTC 118 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGGCGACGGAGGCACAGATACGCAGTATTTT 119 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCCGGGACAGGGGGCGGGAGCAGTACTTC 120 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGTAGGACAGGCAATGAGCAGTTCTTC 121 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGCACCTAAGCGGGGACTACAATGAGCAGTTCTTC 122 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTGCAGGGGCCTGACCCTGAACACTGAAGCTTTCTTT 123 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGGCCAGCCAGGGTGGGGGAAGAGACCCAGTACTTC 124 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTGGGGGCTACAATGAGCAGTTCTTC 125 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGAGTCCAAGAGACCCAGTACTTC 126 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTACGGCAAAGCAGGCAGAACACTGAAGCTTTCTTT 127 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGGAGGACAGCCCTATGGCTACACCTTC 128 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCCTACGTGCGGGCGGCGGACCAGATACGCAGTATTTT 129 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGGGGTAGCCGTGGTGGACGAGCAGTACTTC 130 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGTGGGGATTTGGGGGGACCTACGAGCAGTACTTC 131 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATAGGGCAGCAAGAGCAGTTCTTC 132 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAGGGGGGGGCTCTTGGCTACACCTTC 133 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTCCGGACAGGGGGCCACTGAAGCTTTCTTT 134 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAGTTGGTGTTTACGAGCAGTACTTC 135 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGTTGCCCAACTACGTGCACTGAAGCTTTCTTT 136 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGACCAGGACAGGTTAAACTATGGCTACACCTTC 137 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTGGGGGGGCGATTCACCCCTCCACTTT 138 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTCCTGGACAGCTTGAACACTGAAGCTTTCTTT 139 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATCCAACGGACTCCTACGAGCAGTACTTC 140 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAATTTGGTTACGAGCAGTACTTC 141 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCACTTATAACACCGGGGAGCTGTTTTTT 142 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGGGGAGGGAGGACAGCTAGACGGCTACACCTTC 143 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCACCTAGGGGTGAGCAGTTCTTC 144 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTCGAGCCTCCCAACACCGGGGAGCTGTTTTTT 145 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGCACAAATGAGCAGTTCTTC 146 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGTCGCTAGCGGGGGGCGCGAGCAGTACTTC 147 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGATTCCCCGTTGAACACTGAAGCTTTCTTT 148 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGAGGGGGCTTATACGAGCAGTACTTC 149 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAACGGAGGATAGCAGGTCAAGAGACCCAGTACTTC 150 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCGTCAGGGGGCTCGGGCACTGAAGCTTTCTTT 151 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTTCAATGGACAGGGGTGCAGGAGCAGTTCTTC 152 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAATACCGGGTTGGGGTCACTGAAGCTTTCTTT 153 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCCTTCACAGGGTACACCGGGGAGCTGTTTTTT 154 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCTGGGCGCGGGAGTAGGTGAGCAGTTCTTC 155 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGAGGACAGGGGACGTGAGCAGTTCTTC 156 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTGAGTTAGACCGGGGACGGGACTATGGCTACACCTTC 157 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAGCGCTTCTAGCGGAGACCACAGATACGCAGTATTTT 158 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGGTCAGGGGAGTACATTCAGTACTTC 159 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGAACTAGCGGACCCTACGAGCAGTACTTC 160 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCGCCCGCTTCAGGGGGCACTGAAGATACGCAGTATTTT 161 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCATGAGCGGTTAGGGAATGAGCAGTTCTTC 162 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTGCCGGGACCACAAAAGAGGACGAGCAGTACTTC 163 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGAACCCTGGGGACCGGGGGCCGCTCCTACAATGAGCAGTTCTTC 164 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGAGGGACAGGGCCCATATGGCTACACCTTC 165 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGAAATACAGGGGCCTACCGTTCCTACAATGAGCAGTTCTTC 166 13-TL647-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAAATAGCGGTGAGCAGTTCTTC 167 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGATGGGACAGGGGCCTACGAGCAGTACTTC 168 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTGAGCAGGACCTACGAGCAGTACTTC 169 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTCGAGGTGGGACTTCCAAGAGACCCAGTACTTC 170 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGACCGGGACAGGGCCACAGATACGCAGTATTTT 171 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTATTCAGGGTTTGGGCACAGATACGCAGTATTTT 172 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTGGGGGACGACCTACGAGCAGTACTTC 173 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTCAGGGGGTACGAGCAGTACTTC 174 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGGGCGGGGGGTAATGAGCAGTTCTTC 175 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACACCCCCTACGGGGGGGCCGCGACCCAGTACTTC 176 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGGACCCACGACTACCTGGATAGCGGGGGGGCCGCAGATACG CAGTATTTT 177 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCCGGGACTAGCGGGGGGGCCGTCGGGGAGCTG TTTTTT 178 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCACCCCGGACTAGCGGGGGGCCGGTACCAAGAGACC CAGTACTTC 179 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTAGGACTAGGCAGCCAAGAGACCCAGTACTTC 180 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCTGAGACTGAAGCTTTCTTT 181 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGACCAAGACTCAAAGGCGACGGGACAGATACGCAGTATTTT 182 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCTAGTCGCCTTATAACCAAGGCGAACACCGGGGAGCTGTTTTTT 183 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGAGAATGAGGGTAATGAGCAGTTCTTC 184 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACGGACAGGGAGCCTACGAGCAGTACTTC 185 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCTCGACTAGCGGGGGGCCGTCAAAGCACA GATACGCAGTATTTT 186 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTATTGCAGGGCACAGATACGCAGTATTTT 187 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGGGCGGGTGGCAGCCCGATACTACAATGAGCAGTTCTTC 188 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGAGACCCGACAGGGAGGCGTGAGGACTGAAGCTTTCTTT 189 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCGAGTTAGAGGGGGGTACAATGAGCAGTTCTTC 190 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTTGGAGGGCAGGAGTACTCTGGAAACACCATATATTTT 191 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGACCTAAGGTGGGGACAGTACCAAGAGACCCAGTACTTC 192 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCACGTTGGACCTTACTAGCGGGGGGGAGGATACGCAGTATTTT 193 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGATGGGACGAACACCGGGGAGCTGTTTTTT 194 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCCCTGGGGTAGCGGGGGGCAGGAGACCCAGTACTTC 195 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACCGAGGCGACAGGAACCTCCTACAATGAGCAGTTCTTC 196 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGAAATGGGGCTTATAATTCACCCCTCCACTTT 197 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGACACAGGAGCCCGCCACTATGGCTACACCTTC 198 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGACCAGCATTACAAGAGACCCAGTACTTC 199 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGTTCGGCTACAATGAGCAGTTCTTC 200 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTGGGGACTAGCGGAGACCAATGAGCAGTTCTTC 201 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTACCGGGACAGATGAACACTGAAGCTTTCTTT 202 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTACGAGGAAGGGTACCCAAAAACATTCAGTACTTC 203 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCGGAGGGGGCGCTTGGAAACACCATATATTTT 204 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAGGGGACAGGGACTATATACGAGCAGTACTTC 205 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCGGCAGGGGACCCTTCTGGGGCCAACGTC CTGACTTTC 206 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCGGCTTTCGCGGCGAGCTATGGCTACACCTTC 207 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCGTACAGGACAAATGAAAAACTGTTTTTT 208 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGCCGGGGAGCTGTTTTTT 209 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCGCGCCTCGCCTGACAGGGGGTTTTTGTAC ACCGGGGAGCTGTTTTTT 210 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAGGAGGGTTGGATTCGTATCTTC 211 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACTCGGGGACAGAGCCTCCAAGAGACCCAGTACTTC 212 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCTCCCCCGGGACTAGCGGGGGGGCCTGGG GATACGCAGTATTTT 213 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTATCTAAGGGAAGCAGGGCTAACTATGGCTACACCTTC 214 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGGTGGCGAGCAGTACTTC 215 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCGGACGTGGCCAAAAACATTCAGTACTTC 216 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATACGCTCGGCTTACGAGCAGTACTTC 217 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCCCCCTATAACTCCTACGAGCAGTACTTC 218 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGACAGGGGCTCCCGGGGAGCTGTTTTTT 219 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGCTAGCGGGGGATTTCTCGGAGATACGCAGTATTTT 220 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGTTCGCAGGGGGCGATCACCCCTCCACTTT 221 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAATCACGGACTAGCGGGGGGCGGGGAGAGCAGTTCTTC 222 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACCCACCGGGACCGTAACTACGAGCAGTACTTC 223 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGGGAGCATCAGGGACGAGAGAACACCGGGGAGCTGTTTTTT 224 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTACGGGCCTACGAGCAGTACTTC 225 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACGGGTCAGCTTTACACCGGGGAGCTGTTTTTT 226 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTGGAGGCCAGCTGGAAACACCATATATTTT 227 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGACAGGGGATTCTTCGATGAGCAGTTCTTC 228 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCCCCGGACAGGGGCTACAATGAGCAGTTCTTC 229 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTCCCCCAAGAGACCATGAACACTGAAGCTTTCTTT 230 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCGGCACTTACGGGGCAAACTACGAGCAGTACTTC 231 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCGTGGGACAGTTCTACGAGCAGTACTTC 232 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCCGGGGGGACAGAGGCATGAACACTGAAGCTTTCTTT 233 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTATGGGGCGGACAGACTCATCTACAATGAGCAGTTCTTC 234 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCGCCGGACAGGGGTGCCACTGAAGCTTTCTTT 235 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGATCGAAGGCCTCGATCTGGAAACACCATATATTTT 236 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCAGAAATCAGCCCCAGCATTTT 237 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGAAGACGGGACTAGCGATAGAGAGACCCAGTACTTC 238 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGTGAGTCCGGAGCAGGTTACGTTCCCTACAATGA GCAGTTCTTC 239 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTCCCGGACTAGCGGGGGGGCAGGAGAGCAGTACTTC 240 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTATCGCTCCTCGTAGGAGGGGGAGTCAAAAA CATTCAGTACTTC 241 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGATGGGCCTCTGGATACGCAGTATTTT 242 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTATACGGCCAACTACGAGCAGTACTTC 243 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACCGGACTAGCGGGGGTTTAAACACC GGGGAGCTGTTTTTT 244 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCCGCATTATCTGGTGGGTCTCTCTC TCTGGGGCCAACGTCCTGACTTTC 245 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACGGGCGGGGGGGCCACAATGAGCAGTTCTTC 246 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGTTGGGCCGGGGGCGCGCGGCTACACCTTC 247 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGATCTTAACAGCGTCGCCCCAAGCGGCGAGCAGTACTTC 248 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTACCGGGACAGACTCAATGAGCAGTTCTTC 249 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACGGGTCAGCTTTACACCGGGGAGCTGTTTTTT 250 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGATAGGGGGTGGTGGGACAATGAGCAGTTCTTC 251 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGAGGACAGCGACCGGGGCGAGCAGTACTTC 252 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGATGATGGCTCCGCTTCTTATCTTAGCAAT CAGCCCCAGCATTTT 253 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACGCAACCGACAGGGGGCTTCTACGAGCAGTACTTC 254 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCTCTCGCCCGGAGCCAGTACTTC 255 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGTCGACAGGGCGCTCTGGAAACACCATATATTTT 256 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGACAGGGATTGAAGCTTTCTTT 257 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCATTTATAGAGGCCTTTACGCAGTATTTT 258 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGCATGACAGGGGGCTGGGGTCAGCCCCAGCATTTT 259 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGTAGGGGGCCAAGGCTACGAGCAGTACTTC 260 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGACGACCCGGGGCTTGGCACAGATACGCAGTATTTT 261 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGAAATCAATGAGCAGTTCTTC 262 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAAGGGACAGGGGATTTATGGCTACACCTTC 263 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCACATCTGGGACAGATACCTTACAATGAGCAGTTCTTC 264 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCCCCTTGGCGGGGGCATGAGCAGTTCTTC 265 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCTCTTTGGTGGGCTCCTACGAGCAGTACTTC 266 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCTCGCCTCCCACGGCGGAGGGATACTACGAGCAGTACTTC 267 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCACCCGGGACACGAGTCCTACGAGCAGTACTTC 268 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCTAGGACAGACGGCGCAAAAACTGTTTTTT 269 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCAACGGCGATGAGCAGTTCTTC 270 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGAATTGACGGAAGCTTTCTTT 271 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCTAGGAGCAATCAGCCCCAGCATTTT 272 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCAGAGGGGCGACCTTCTACGAGCAGTACTTC 273 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCGGCAGGGGGCCCTTCTGGGGCCAAC GTCCTGACTTTC 274 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGAGTGGGCAGGTAGCAATCAGCCCCAGCATTTT 275 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCTCACCATTCCCCCCCCAGAGCTCCTACAATGAGCAGTTCTTC 276 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGGGGCCCTCAGGGGTACTACGAGCAGTACTTC 277 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTAGGGGTCCCGACAGGGGGAGACTCACCCCTCCACTTT 278 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACACGCCTTGGGCAGGACCCTACGAGCAGTACTTC 279 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCATCCTACGGGGGGACTACAATGAGCAGTTCTTC 280 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATCTTAGGACTCACCGGGGAGCTGTTTTTT 281 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGGACTAAATTCACCCCTCCACTTT 282 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGCGCGGGTCGCGGTAGCGGGGGGAC TAAGCTCCTACAATGAGCAGTTCTTC 283 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGTTAGGCCCCGTCGGCAGGGGTGATGACGAG CAGTACTTC 284 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGTGAAATGGGGGGGGGCCAAGAGACCCAGTACTTC 285 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTCGCGACAGGGGGCCCAGAGACCCAGTACTTC 286 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACTCCTTGGACAGGGGGCTCCAACTCCTATAATTCAC CCCTCCACTTT 287 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCCGTCGCGGGGGGGGACAATGAGCAGTTCTTC 288 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCTAGCGGGGGGCCCTACAATGAGCAGTTCTTC 289 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGTGCCTAGCGGGGGAGAGACCCAGTACTTC 290 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGCCGTGGACAGGGACGACGAGCAGTACTTC 291 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCATTAGTGCGGGGGGCGCATGGTCAGCCCCAGCATTTT 292 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGACATGGGGAGGGGTGGCGAGCAGTACTTC 293 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGGGACTAGCAATGAGCAGTTCTTC 294 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGACTTGTCTTCACCCCTCCACTTT 295 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTTACGGACAGGAATCGAGACTACGAGCAGTACTTC 296 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACCGGACAGCCTTTGGTAGCACAGATACGCAGTATTTT 297 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCTTTGGGGTCCGGGACTGTAGCGAGGGCTAGAGA CGAGCAGTACTTC 298 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCCAAACGGCGGCAACTAATGAAAAACTGTTTTTT 299 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCGGAAACGGGAACACCGGGGAGCTGTTTTTT 300 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCATCCCGTCCCGACCTGGCACAGATACGCAGTATTTT 301 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTTTCCGGGCGGGGGGAACACTGAAGCTTTCTTT 302 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGAGTTGCAGGGTCATAATGAAAAACTGTTTTTT 303 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCCCCCGTGGTGGAGACCCAGTACTTC 304 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGAACATGCGAACACTGAAGCTTTCTTT 305 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACCCAAGGGGGGGCCGGGACCCAGTACTTC 306 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCATCGAGACAGGGGGGACACTGAAGCTTTCTTT 307 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACGGGAGCCTGGGCGGGGAGCTGTTTTTT 308 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAACTTCCGGGACAGGCCGTACAATGAGCAGTTCTTC 309 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAATCTAGCGGGGGGGCAGATACGCAGTATTTT 310 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTAGTCGGAGCTCCTACGAGCAGTACTTC 311 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCACAGGGGCCCTCCTACGAGCAGTACTTC 312 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGGCTGGGACTACAAGGATCTAGCACAGATACGCAGTATTTT 313 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGTACTGCGGGGTACACCGGGGAGCTGTTTTTT 314 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGTGAAGACGGTATGAACACTGAAGCTTTCTTT 315 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAACAGGGAGGATGCAGTTAGCACTGAAGCTTTCTTT 316 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTATGCGTCCCCCACTGAAGCTTTCTTT 317 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCGAGTTGGAACCGGGGAGCTGTTTTTT 318 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCCAAAAGGAACCGATCACCCCTCCACTTT 319 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCCACACCGGACCTCTACAATGAGCAGTTCTTC 320 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGACCCCCCAGGGACAACATATATCGATAAT TCACCCCTCCACTTT 321 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCAGGGGTCGCTGGCTACACCTTC 322 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAACCTAGGACAGGGGGAAACAATGAGCAGTTCTTC 323 139-TL706-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTATTCGCAATAGAGCAGGGGAACACCGGGGAGCTG TTTTTT 324 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGACCGGGACAGGGCCACAGATACGCAGTATTTT 325 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCACCCCGGACTAGCGGGGGGCCGGTACCAAGAGAC CCAGTACTTC 326 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTGAGACTGAAGCTTTCTTT 327 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAGAAACAGTCTCTAATGAAAAACTGTTTTTT 328 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCACATCTGGGACAGATACCTTACAATGAGCAGTTCTTC 329 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGAGACCCGACAGGGAGGCGTGAGGACTGAAGCTTTCTTT 330 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGAGGCCCTAGCTCAGAACAATGAGCAGTTCTTC 331 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGGGCGGGTGGCAGCCCGATACTACAATGAGCAGTTCTTC 332 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCATTTATAGAGGCCTTTACGCAGTATTTT 333 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCAAATTTGATGACAGAAGCAAAAGCTAACTATGG CTACACCTTC 334 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGGCACTTACGGGGCAAACTACGAGCAGTACTTC 335 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTCGACTAGCGGGGGGCCGTCAAAGCACA GATACGCAGTATTTT 336 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTACCCCCCCAGGGATGGGGGTCGCGACTAAT GAAAAACTGTTTTTT 337 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCCGGACGTCTCTCTGGGGCCAACGTCCTGACTTTC 338 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGGCCCGGGTACCAAGAGACCCAGTACTTC 339 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGGACCCACGACTACCTGGATAGCGGGGGGGCCGCAGAT ACGCAGTATTTT 340 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTTGGAGGGCAGGAGTACTCTGGAAACACCATATATTTT 341 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAGGAAATGACAGGGTTGTCCTCCACAGATAC GCAGTATTTT 342 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGATGGGACAGGGGCCTACGAGCAGTACTTC 343 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGAATTCCGCGGGGTACAGTAGAGCTGTTTTTT 344 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAGAGGCGGCGGGAACAGATACGCAGTATTTT 345 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCCCGAGGGACGGACGCAGATACGCAGTATTTT 346 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTGAAGACGGTATGAACACTGAAGCTTTCTTT 347 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTCCCCGACAGGTATGAACACTGAAGCTTTCTTT 348 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCGTACAGGACAAATGAAAAACTGTTTTTT 349 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGAACAACGCGGGGGGCTGGGACAATGAGCAGTTCTTC 350 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGCCGGCCAGCGGGGGGGCCGTAGGACAG ATACGCAGTATTTT 351 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGACAGGGATTGAAGCTTTCTTT 352 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTGGACAGGGGGCCAAGAGACCCAGTACTTC 353 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAAGGGACAGGGGATTTATGGCTACACCTTC 354 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGTCCGGGGCCTCCTATGGCTACACCTTC 355 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTAAACAGGATTACTATGGCTACACCTTC 356 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCCAAAGGACAGGGGGTATCGCTGAAGCTTTCTTT 357 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCGACTTAGATGAGGGCTTGAACACTGAAGCTTTCTTT 358 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTCGGACAGGGGCCGATGCGGAGCAGTTCTTC 359 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTACGACAGGTTCGACGAGCAGTACTTC 360 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCGGACGGGACAGCCTGGACTATGGCTACACCTTC 361 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGACAGGTCCCGGCAATTCACCCCTCCACTTT 362 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGTCGACAGGGCGCTCTGGAAACACCATATATTTT 363 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGGACTAAATCACGAGACCCAGTACTTC 364 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGGTGGCGAGCAGTACTTC 365 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAGGGGAGGTGGCAGATACGCAGTATTTT 366 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAATCACGGACTAGCGGGGGGCGGGGAGAGCAGTTCTTC 367 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAAACCCAAAAAGGGACAGGGGAACACCGGGGAGCTGTTTTTT 368 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCCAGCCCTATATCGGAGTTCTTC 369 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCGAGAAGACACGGCCGTGGATGGCTACACCTTC 370 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAAGCGGGGGTCTAGAAGATACGCAGTATTTT 371 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCACGCCGGGACAGGGACTCTACGAGCAGTACTTC 372 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGTCCGATTAATTAGGACTAGCGGCGACTAC GAGCAGTACTTC 373 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAATAGGGACAGGGTTGACCGGGGAGCTGTTTTTT 374 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTGCAGGGTGGTCGATATGGCTACACCTTC 375 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCACGGACAGGGGCTGGTCACTGAAGCTTTCTTT 376 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGGCACAGGGGCCGAGCAGTACTTC 377 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCCCAGTGACGCAGGGAAGGAAC ACCGGGGAGCTGTTTTTT 378 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGGGACAGGGACTGCCTACGAGCAGTACTTC 379 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGACCCCCCAGGGACAACATATATCGATAA TTCACCCCTCCACTTT 380 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGACCAGCCAAGATATAGCAATCAGCCCCAGCATTTT 381 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTCGGGACAGGGATGGCAGATACGCAGTATTTT 382 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTGGGGCGGGGGGCATACAGATACGCAGTATTTT 383 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGATTCGGCCAGCAATTCACCCCTCCACTTT 384 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAATCAAGGGGGCGGAGGAGACCCAGTACTTC 385 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTCGGCGACTAGCGGGGGCCCTAATGGATACAAT GAGCAGTTCTTC 386 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCGGGACACCCACTGAAGCTTTCTTT 387 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTGGACAGGGGGTGAAGTACGAGCAGTACTTC 388 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCTCTCTCCCCCAGGGGGATGGCTACGAGCAGTACTTC 389 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAGAGATGGGGACCCCTCTCCTACGAGCAGTACTTC 390 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATAGCGGAGAACACTGAAGCTTTCTTT 391 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTACAGGGAAACACTGAAGCTTTCTTT 392 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCCTGGGGACTAGCGGGGGCCGAAGAGACCCAGTACTTC 393 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTAGCGACTCTAGCACAGATACGCAGTATTTT 394 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAAGAACACCGGGGAGCTGTTTTTT 395 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACCGGCAGGGACTTTGGGGCGAGCAGTACTTC 396 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAGGACTAGACGGTTACGAGCAGTACTTC 397 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGCGACAGGATTGGCAACACTGAAGCTTTCTTT 398 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGGCAAGACAGGCTCGAGTCCATGAGCAGTTCTTC 399 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGACATGGACAGGGGGATAATTCACCCCTCCACTTT 400 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACGATCTAGCGGGGGGAGACGAGCAGTACTTC 401 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGGTGGGGGACTAGCCCCTTCGGTGTCCTAC AATGAGCAGTTCTTC 402 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGAGGGCAGGGGCTGGACTGAAGCTTTCTTT 403 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCCCCTCCCGTCTGGGGGCCCGGCCCCCAGATACG CAGTATTTT 404 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTACCTGGGACAGGGGGAATAGTCTCCCTGAAGCTTTCTTT 405 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGGGAAACAGGAGAACTATGGCTACACCTTC 406 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGTTTCCAGGGGGTAAAGGGGGATTTTATGAAAA ACTGTTTTTT 407 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATACGCTCGGCTTACGAGCAGTACTTC 408 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTGGGGCAGCTACGAGCAGTACTTC 409 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGGGACGGGGACGGACACTGAAGCTTTCTTT 410 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTGCCTTGGACAGGTGCTTATGGCTACACCTTC 411 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGTACCGGGCCGGAGCACCGGGGAGCTGTTTTTT 412 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGACCAGGGATCTGGGTCACCCCTCCACTTT 413 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCCCCGCCCGTGAGCAGTTCTTC 414 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGTGGGGCTAGGTTTAACTACGAGCAGTACTTC 415 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTACCGGGGCATATGGCTACACCTTC 416 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCCCGTAGGGACCTCCTACGAGCAGTACTTC 417 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAGACAGGGAGGGAAGAGACCCAGTACTTC 418 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCTCGACAGGGACCCTCCAATGAGCAGTTCTTC 419 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGCCGCAGCCAGGGTGGGACCAAGAGACCCAGTACTTC 420 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAATGGGGGGCACAGATACGCAGTATTTT 421 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCGGGGGGAGGGTGGCCTTTGAATGAGCAGTTCTTC 422 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGGCGCTCTGGCCAACACTGAAGCTTTCTTT 423 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTACAACAGGTACCCATAGCAATCAGCCCCAGCATTTT 424 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATAACAGGCGTCCGCACAGATACGCAGTATTTT 425 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGGGGGGGTCAGCACAGATACGCAGTATTTT 426 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATACGGTCCTCCTACGAGCAGTACTTC 427 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGCAGACAGGGAGAAATCAGCCCCAGCATTTT 428 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGTGACGAGACCACTGAAGCTTTCTTT 429 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTCACAGGACAGGGCTACAATGAGCAGTTCTTC 430 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGGGGGGGAGAACACCGGGGAGCTGTTTTTT 431 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCGCCGACAGGGTGGGGATATAATTCACCC CTCCACTTT 432 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGGGGACGCGGGGAATCGTACAATGAGCAGTTCTTC 433 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCCCGGGGGGAGCTCCTACAATGAGCAGTTCTTC 434 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTGGACAGGGGCGGGGAACACTGAAGCTTTCTTT 435 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACCGACGGGTTCCTGGGGCCAACGTCCTGACTTTC 436 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATGAGTATGAGCAGTTCTTC 437 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTATCGGGTGGTGGTGAAGCTTTCTTT 438 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAGGGGACAGGTTTCGAAAAACTGTTTTTT 439 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAATCTCCGGGGGTGGCACCGGGGAGCTGTTTTTT 440 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGCGGTGGGGACAGGACTCACCGGGGAGCTGTTTTTT 441 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCAGGAAACGAGCAGTACTTC 442 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCGAATCTAGCTCTCAATGAGCAGTTCTTC 443 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGGGTTTCGACCCAATAGCAATCAGCCCCAGCATTTT 444 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGTTGGGTCGCACGAGCAGTACTTC 445 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGATGCAGGGGCCGCCTACGAGCAGTACTTC 446 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAACGGGGACCAGGGGAGAGACCCAGTACTTC 447 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTTCTTGAAGAGACCCAGTACTTC 448 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCGACTGCGGGGGCCCCCCGGATCTTC 449 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGGGTCAAAGGGACAGGGGGTCATCAGCCCCAGCATTTT 450 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCACGGCAGCCAAGAGACCCAGTACTTC 451 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGTTAGCGGGGGCAGGTTGGACACCGGGGAGCTGTTTTTT 452 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCATAGCGCGGGGGCTAATGAGCAGTTCTTC 453 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCCAAACGGCGGCAACTAATGAAAAACTGTTTTTT 454 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGAGAGGAGACAGGGCCCAACTACTACGAGCAGTACTTC 455 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGGTATGAGACAGGGTGGTTTAGGCACAGATACGCAGTATTTT 456 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCGGGGGGGTCCTACGAGCAGTACTTC 457 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCTGGAGGGACAGGGGCCGCTGAAGCTTTCTTT 458 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTATGGGCGGGGGGGCCACAATGAGCAGTTCTTC 459 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTGTCGCCGGGACAGATGAGCAGTACTTC 460 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGCAGGGGCTATTACGAGCAGTACTTC 461 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCCCCCAAGAGACCATGAACACTGAAGCTTTCTTT 462 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGTCGCAGCCACAGATACGCAGTATTTT 463 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTGGACACGGAACACTGAAGCTTTCTTT 464 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTTCTGGGACAGGGGACACCGGGGAGCTGTTTTTT 465 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCAGGGGGACAGGGGACGGTTAACTATGGCTACACCTTC 466 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGGGACAGGGTGGGTAATGGCTACACCTTC 467 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTATTTGGGGCCTACGAGCAGTACTTC 468 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCACCGGGACAAGCTACGAGCAGTACTTC 469 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGATGGGCGGGAGAAACACCGGGGAGCTGTTTTTT 470 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTATACGGGGGTGAGGGAGAGACCCAGTACTTC 471 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTGGGGGAGAGCAACTAATGAAAAACTGTTTTTT 472 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCTGGACGGCTCTCTGGGGCCAACGTCCTGACTTTC 473 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATAGGGAGTCATCGAACACCGGGGAGCTGTTTTTT 474 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCGGACGACTAGCCGATAGCACAGATACGCAGTATTTT 475 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATCGCGGCGGGAAAAAGAGACCCAGTACTTC 476 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCACTATAGCTCTCGGGACAGGGTTCGGCTACACCTTC 477 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCACGACTAGCGGGGGTTGAGCAGTACTTC 478 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGTTAGGCAGGGGTATGGCTACACCTTC 479 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCCGGAGAGGCAGGCCAGCCTACGAGCAGTACTTC 480 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATAATTTGGGGGACCGGGGAGCTGTTTTTT 481 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCACGACGGGGGGCTACAATGAGCAGTTCTTC 482 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCACCCCGGACTGAGCTACGAGCAGTACTTC 483 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCAATACAGGGGCCTATGGCTACACCTTC 484 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCGCTAGCGGGGACTCCTACGAGCAGTACTTC 485 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGCCCCGGGGGAGGTGAAAAACTGTTTTTT 486 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGTGGGACGGAGCGATACACCTTC 487 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATAGCAGGGGCAGATACGCAGTATTTT 488 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTTCTCAGGCTCAATATGGCTACACCTTC 489 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATCGCAGGGGGTTCTTGAGACCCAGTACTTC 490 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGAGGCAAGCCCTGAAGCTTTCTTT 491 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTCAAGAGGGGAACATCTACGAGCAGTACTTC 492 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGGGCAGTAACACTGAAGCTTTCTTT 493 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAGCTCCTACGAGCAGTACTTC 494 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTCAGGGGACAGGGGGAATCTACGAGCAGTACTTC 495 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCGGGACAGGGGACGTGGAACTATGGCTACACCTTC 496 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCTGTACAGGGTGCGAACCTCCCGGGGGAAAAACTG TTTTTT 497 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTACTTAGTCCTAGCGGGGGCCAAGAGACCCAGTACTTC 498 140-TL706-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAAGTTCCAGGGATTATGTGGGGTACACTGAAGCTTTC TTT 499 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCTCACCACCTGACAACCCAACTAATGAAAAACTGTTTTTT 500 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGTGCCTCCCGGGGGCCCAGATACGCAGTATTTT 501 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAACTGCAGGGAGAGATACGCAGTATTTT 502 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCGGATCACCGTCTTAACTATGGCTACACCTTC 503 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACCGGGGGAGACCGCTACTATGGCTACACCTTC 504 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGATGCCAATGAAGCTTTCTTT 505 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTATCACTGGAATGAGCAGTTCTTC 506 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGTAAACAGGGGGGGAACACTGAAGCTTTCTTT 507 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGTAGCCAATGAGCAGTTCTTC 508 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGATCAGGGGCTCCACTTCAGGGAGACCCAGTACTTC 509 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCATCGCTAGCGGGGCTAGCACAGATACGCAGTATTTT 510 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGTCCGCCAGATAACTATGGCTACACCTTC 511 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCTTTCGGACGATCTTCCCGAAAAACTGTTTTTT 512 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGAGAACTAGCGGGGGGACTCACGGATACGCAGTATTTT 513 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTCCGCTCATCTCCTGGAACATTCAGTACTTC 514 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGGGACAGCCCTCTGGAAACACCATATATTTT 515 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGACCAGAACCCTAACTATGGCTACACCTTC 516 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGATTCAGGGATGAACAATGAGCAGTTCTTC 517 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGGTAACGGTCCGTAATGAAAAACTGTTTTTT 518 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTAGCCGGGACAGACAATGAGCAGTTCTTC 519 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGATTCGGACAGGCCTCACAATGAGCAGTTCTTC 520 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTGGGGAACTGAAGCTTTCTTT 521 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTAACCGGGACAGCAAATTCTAACTATGGCTACACCTTC 522 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTAGGGAGTGATGGGGTCTATGGCTACACCTTC 523 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCGGGCGAGTACGAGCAGTACTTC 524 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGATTGGAAGTTGGATCTCTACGAGCAGTACTTC 525 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCTGGGGGACAGGGGACACTGAAGCTTTCTTT 526 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTTGCCGGGCCCCCTTACTTC 527 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCAGGGACTCTGGAAACACCATATATTTT 528 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCCGTGCGGCTAGCGGGGGCTGAGCAGTTCTTC 529 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCAAAGGTTTCAGCCGCACCACTTATAATTCACCCCTC CACTTT 530 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTGGGGGGCAGGCTCCAGCTATGGCTACACCTTC 531 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCAGACCTGAACACTGAAGCTTTCTTT 532 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGAGTCTGGGGGGACAGGTTCTCCCTACGAGCAGTACTTC 533 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAACCGTCCTCCGGGCACCCACGGATGGCTACACCTTC 534 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACCAGGGGACAGGAGGATTAAGAGACGAGCAGTACTTC 535 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACGCCTCGGGTCCTGTGCATTTT 536 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCAGATCCCCGCCCCTGGGTAGCGGAGCCCAAG AGACCCAGTACTTC 537 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGACAGTTATCTGGAAACACCATATATTTT 538 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCCGGACAGCGTCGGCCCCAGCATTTT 539 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACGGGACGGAGCACCGGGGAGCTGTTTTTT 540 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGACGGGGGGGCAGACGTTCGGTACCAA GAGACCCAGTACTTC 541 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGACTAGCGGGCCCTACAATGAGCAGTTCTTC 542 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCAGTCGACTGGCGGATACGCAGTATTTT 543 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGGGGCCAGCGGGGGACTCCGAGCAGTTCTTC 544 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGCCCCCCGGATCTTTATGGCTACACCTTC 545 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGAGGGGACAGGCTATCAGCCCCAGCATTTT 546 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAACGAGTACTTTAGAAATCAGCCCCAGCATTTT 547 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACCGAGACTAGCGGGGGATACAATGAGCAGTTCTTC 548 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGATGGGGTGAGACTAAACATTCAGTACTTC 549 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGAACTAGCGGGGTTCTCCAATGAGCAGTTCTTC 550 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCTCCGGGATTGGCTACACCTTC 551 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGCCCTGTCCGAGCGAAGTACTATGGCTACACCTTC 552 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGACTAGCAACACCGGGGAGCTGTTTTTT 553 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCTGAGGGAAGAATTGAACACTGAAGCTTTCTTT 554 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCCTCGGACAGGACTGGCAATGAGCAGTTCTTC 555 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCGGGACAGGGGATGCGACCCAGTACTTC 556 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTCACCCCCAACACCGGGGAGCTGTTTTTT 557 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCTACAGCAAACAATCAGCCCCAGCATTTT 558 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAAGAATCGGGGGGTCACTTTCACCAATC CAGATCTACGAGCAGTACTTC 559 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGATGGGGGACACCGGGGAGCTGTTTTTT 560 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTGCGCCCGGGACTAGCGGGGGGCCCAGAT ACGCAGTATTTT 561 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATAGGGACGTACGCGAACACCGGGGAGCTGTTTTTT 562 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCTGATCCCCGGGGCCACAGATACGCAGTATTTT 563 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGGGACTAGCGGGGGGGCAGATACGCAGTATTTT 564 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCTGATGGCAGATACGCAGTATTTT 565 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAGCGTTGGGGGAGCACAGATACGCAGTATTTT 566 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGTTCGAGGGGGCCCAAGAGACCCAGTACTTC 567 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTGGGGGGACAGAACTATGGCTACACCTTC 568 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTTGGGGACACGAACACTGAAGCTTTCTTT 569 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCATCGGGGGGCACTGAAGCTTTCTTT 570 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAAAAACCACGGGACAGGCTTAGACGAGCAGTACTTC 571 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTACTGGACAGGCAGAAAAACTGTTTTTT 572 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTAGCCCCCCAGGGGGCGAGTTCTGGCTACACCTTC 573 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCGGGACGGGATCCTACGAGCAGTACTTC 574 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTCCTTCCCGACAGTCCCCAACGGGCCCAGTACTTC 575 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTAGCGGGCGGGGGCTCCTACGAGCAGTACTTC 576 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGCGTACCTCTGTGAACGTCCTGACTTTC 577 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAACTAGCGGGGGGGCGCGATGAGCAGTTCTTC 578 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCGAATGGGGGGTGGAACTATGGCTACACCTTC 579 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCGGGACAGGGGTATCACAGATACGCAGTATTTT 580 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGATTGGAGTGGCATCCCCGGGGAGCTGTTTTTT 581 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCCTGGGGTTCTGAAGCTTTCTTT 582 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGCTCGGGGTTCACGGCCTGAACACTGAAGCTTTCTTT 583 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTACGGCTCCCGGGACTACCTCCTACGAGCAGTACTTC 584 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCTCACCTTACCCCTTGGGAACAGATACGCAGTATTTT 585 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCTGGGACAGGGGAACACTGAAGCTTTCTTT 586 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCGCCGGATTGACCTTGGCGAAGAGACCCAGTACTTC 587 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCTCAGGGTCCCCAATTGGCCCAGCATTTT 588 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCACTCCGGGACAGGGTTCCCCTGGCCTTC 589 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTTGGCGGGGGGACCTACGAGCAGTACTTC 590 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCCCTCGGGGGTACCAATCTTGCAGATACGCAGTATTTT 591 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTTGGTCCGTTTGGCACCGGGGAGCTGTTTTTT 592 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTACAGGGGGCGCTAGAAGGCTACACCTTC 593 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCAAGACTAGCGGGGGGTATAGCACAGATACGCAGTATTTT 594 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCATTACAGGGGGGGATCAGCCCCAGCATTTT 595 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCTGGGGCTTCAAGAGACCCAGTACTTC 596 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCGGAACGACAGGGTACGGGAAGAGCAATCAGCCCCAGCATTTT 597 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTATACGGGGCGGGTGAGCAGTACTTC 598 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTCCCCGGTCAGGCCAGATACGCAGTATTTT 599 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGTAGCCAATGAGCAGTTATTC 600 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGCAGGGGGATCCTACAATGAGCAGTTCTTC 601 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATTGGACAGGGGTACGAGCAGTACTTC 602 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCACCAAGCCGATGAGCAGTTCTTC 603 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCCTTAGGTGTAAGCGGGGCAAGCTCCTAC AATGAGCAGTTCTTC 604 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGGTACAGGGGCGTCTAATGAAAAACTGTTTTTT 605 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCAGCGACTAGCGGGGGCCGGGACGAGCAGTACTTC 606 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTATTTGAGGACAGGGGGCTAAGAGAGACCCAGTACTTC 607 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCTGGACAGACAGATACGCAGTATTTT 608 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCAAAACGCCTACAGGGGGAAGCCCCAGCATTTT 609 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCTGTCCGGGGGGGGAATGGGTGAGCAGTTCTTC 610 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCCGTAGGGGCTAGAGAGCAGTACTTC 611 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCGAACTAGCGTCCGGGGAGCTGTTTTTT 612 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGGACAGGGGGGGTCAGCCCCAGCATTTT 613 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGAGATGCAGGGGCGGGAGGCTACACCTTC 614 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCCCAACGGGGGCCTATGGCTACACCTTC 615 151-TL722-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCGGACTAGCGGGGGGGCGGATGAGCAGTTCTTC 616 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCGGGGGAGACCGCTACTATGGCTACACCTTC 617 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGACGACCGGGACAGGGATGGATTCCGATACA ATCAGCCCCAGCATTTT 618 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGATTCGGACAGGCCTCACAATGAGCAGTTCTTC 619 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAAAGGTTTCAGCCGCACCACTTATAAT TCACCCCTCCACTTT 620 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCGGGTATAGTAGCAATCAGCCCCAGCATTTT 621 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGGGCGAGTACGAGCAGTACTTC 622 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGAGGGGCAGGAGACAGATACGCAGTATTTT 623 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGGGGTTGGGACAGGGGAACCTACGAGCAGTACTTC 624 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTGCCGGGCCCCCTTACTTC 625 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGGACAGGATCTAACTATGGCTACACCTTC 626 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATAGACGGCACTTCCTACGAGCAGTACTTC 627 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTGGGACAGGGGGGCACTAATGAAAAACTGTTTTTT 628 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGGACGGTTAGCGGACACCGGGGAGCTGTTTTTT 629 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGACGGGACGGCTACACCTTC 630 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCCGGGACAGCACCTACGAGCAGTACTTC 631 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAATAATCCTTGCCTACGAGCAGTACTTC 632 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAGGGGACACGCAGTACTTC 633 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTGAGGACGGTATGAACACTGAAGCTTTCTTT 634 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTATACGGCGAAGATCCTGACTTTC 635 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTAGAGCAGGGGAAACCAACTATGGCTACACCTTC 636 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGAGTGGGACAGGCGCCTACGAGCAGTACTTC 637 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGACGGCTAGCGGGCACCGGGGAGCTGTTTTTT 638 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCAGCCCCAGGAGGCCAGCCCCAGCATTTT 639 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTAACCGGGACAGCAAATTCTAACTATGGCTACACCTTC 640 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAGGAAGGGGAGTAGAGACCCAGTACTTC 641 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGCAAGAGTCGGGGCAGCCCCAGCATTTT 642 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGACAGGGGTTCCTGAAAAACTGTTTTTT 643 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTGGTCCGTTTGGCACCGGGGAGCTGTTTTTT 644 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGATCCGGGACAGGGGAATGAGCAGTTCTTC 645 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCTTGGGACAGGATAAAGGAGCAGTACTTC 646 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCGGGGGAGGGGGCTGGTACAATGAGCAGTTCTTC 647 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCGGGACAGGGGGCAGGCCCCAGCATTTT 648 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGATGGACAGGCCAACGTCCTGACTTTC 649 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTGTACAGGGGACCGATACGCAGTATTTT 650 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGAGACAGGGAGACTACGAGCAGTACTTC 651 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGGGAGCAGCGGAACTAATGAAAAACTGTTTTTT 652 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGACAGGTGGGGACAATCAGCCCCAGCATTTT 653 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAGTAGCGGGTACCAAGAGACCCAGTACTTC 654 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCTCTTCCTTTGGACGGGGAGCTCCTACAAT GAGCAGTTCTTC 655 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGGGAATGAAGCTTTCTTT 656 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCGACGACCCGTTCCGACTCCTACGAGCAGTACTTC 657 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCCCTGCCCGGGCGGGGGCGCGAGCAGTACTTC 658 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAGACAGGGGCCGAGAGACCCAGTACTTC 659 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGGGGCGGCTCCTACGAGCAGTACTTC 660 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAAGTCCGATCACCGGGGAGCTGTTTTTT 661 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATAGATAATACAGGGCGCAATCAGCCCCAGCATTTT 662 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCGGCCCGGGGGTCCCACCGTACGATACGCAGTATTTT 663 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAGCTCAAATCAATGGGCTATGGCTACACCTTC 664 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGAATCTACCCTTTCTGGCACGGACACAGA TACGCAGTATTTT 665 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGCGTCGCTTAGCACAGATACGCAGTATTTT 666 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCCGGACGGGCTCCTACGAGCAGTACTTC 667 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCGGGAACAGCCGGCTTACGCAGTATTTT 668 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCCAGACAGGGAACAATCAGCCCCAGCATTTT 669 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCACATCGACAGGGATGGCTGAAAAACTGTTTTTT 670 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTCCCGGACAGGGGGCGACAGATACGCAGTATTTT 671 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATAGATCTATATAGCAATCAGCCCCAGCATTTT 672 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGGGGTTTGCCAAAAACATTCAGTACTTC 673 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGGGACAGGGAACTACGAGCAGTACTTC 674 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTCTCCCGGGACAGGGGAACACCGGGGAGCTGTTTTTT 675 152-TL722-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAATGGGCTAGCGGGGAGACCCAGTACTTC 676 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGATCTCGGTAAGCAGCCCCAGCATTTT 677 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTATCGTTGACCTACGGTAGAGGGCAGCCCCAGCATTTT 678 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTACGACCCACCCTAATCAGCCCCAGCATTTT 679 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGAGGAACAGGGGGCGCTGAACGAGCAGTACTTC 680 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACGAGGTGGGACAGGGAGCCCGAAGGGTACGAGCAGTACTTC 681 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTCGGGACACGTCTATGGCTACACCTTC 682 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTTACAGGGGATGAACACTGAAGCTTTCTTT 683 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGCTACGAGCAGTACTTC 684 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCCCGTCCGTTGGGACAAATCTACGAGCAGTACTTC 685 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTTTCGGCCGGGCTCAATCAGCCCCAGCATTTT 686 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAATGCATATGATAATTCACCCCTCCACTTT 687 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTTGGACTAGCGGTCTACGAGCAGTACTTC 688 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGAGCCCGGGACTACCCGGGTCGATGAGCAGTTCTTC 689 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCGCCCGGGAGGACGCCAGGAAACACCATATATTTT 690 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTCCGGGGACAGGTTCTGAACACCGGGGAGCTGTTTTTT 691 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCTCACCGGTACAAACAGATACGCAGTATTTT 692 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGGCCCGCTAGCGGGAGGACAGATACGCAGTATTTT 693 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAAAGCTACGAGCAGTACTTC 694 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCCGGGACTAGCGGAGAGCTCCTACGAGCAGTACTTC 695 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTTTCGCGACACTTCTCCTACGAGCAGTACTTC 696 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGGGGCAGCTCCTCAGGGGATGGCTACACCTTC 697 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATAGGGGGAGCTAATGAGCAGTTCTTC 698 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCCCCCGGGGGGCGGACAGGACCTATAAC TATGGCTACACCTTC 699 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCGGACTGGACTACGAGCAGTACTTC 700 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCGTACGGGGACGCTTTGGGAGAGACCCAGTACTTC 701 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCTGGAGGGGACGGATACGAGCAGTACTTC 702 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGGAACAGGGCTCTATAATTCACCCCTCCACTTT 703 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCATGGTAGCGGGAGGTACCTACGAGCAGTACTTC 704 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCTGGAATTTTGGACAGGGGATATCCTACGAGCAGTACTTC 705 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGTTCTGAACACTGAAGCTTTCTTT 706 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGCGGGAGGGATCTCCTACGAGCAGTACTTC 707 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGATTGGGGGACTAGCGGGGGGTCTTGGAG TGAGCAGTTCTTC 708 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGCCTTAATGCGAGAGGCTGAAGCTTTCTTT 709 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTGGGGGACAGTTAATGAGCAGTTCTTC 710 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCAGAGAGCGGACCCCCACAGATACGCAGTATTTT 711 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCGTACGAGCAGTACTTC 712 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTATGGGGGGGCAGTGAAGCTTTCTTT 713 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGGGGGGGCACAGATACGCAGTATTTT 714 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCCCCACTAGCGGCCAAGAGACCCAGTACTTC 715 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCCCTCGGATGGACTGCCGTACCAAGAGACCCAGTACTTC 716 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTGCGGGAAACACTGAAGCTTTCTTT 717 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGTCCGGGACAGGGCTTCCGGGGGGGAGACCCAGTACTTC 718 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCAAGTGCAGGGGTTGAGCAGTTCTTC 719 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCACGAAGGGACCGATTCCACCTACTATAATTCACCC CTCCACTTT 720 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCGGACAGGGGAGAGCAGATACGCAGTATTTT 721 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGGATGTCGGGAACATTCAGTACTTC 722 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCGCCCTCAGGGGGCGGGGAGACCCAGTACTTC 723 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTACCCCGACAGGGTTAAACACTGAAGCTTTCTTT 724 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGATAGGGGGACAACCGGGATGAACACT GAAGCTTTCTTT 725 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTAGCGTCGCAGGAACAAGAGACCCAGTACTTC 726 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGCATTATCCACAGATACGCAGTATTTT 727 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGATTGGGGCGGAGGCTCCTACAATGAGCAGTTCTTC 728 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCATGGGTGGATACGAGCAGTACTTC 729 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCAGTGGGACTAGCGGGGGTTACGAGCAGTACTTC 730 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTTCAGGACTAGCTGGGATCTACGAGCAGTACTTC 731 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCTTCGGCTAGCGGGCTAAATGAGCAGTTCTTC 732 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCTCAACCCGGGACTAGCGGGAGAGACCCAGTACTTC 733 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCTCTCAGGGGAGGAACGAGCAGTACTTC 734 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGAGGAACAGGGGGCGCTGAACGAGCAGTACTTC 735 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTCTTCCGAGGGCGGGAGAAATCTACGAGCAGTACTTC 736 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGTTCACAGCCTCTCCTACGAGCAGTACTTC 737 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCCCTGTCGTCGGCGGTGGCGTACAA TGAGCAGTTCTTC 738 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGATCTCGGACAGTACACAACAAATCAGCCCCAGCATTTT 739 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGGGCTACGGGGGCGCGACTGAAGCTTTCTTT 740 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCAAAGCGGGGGGCTATCCTACAATGAGCAGTTCTTC 741 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGAGGGCGGATTGAACACTGAAGCTTTCTTT 742 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCCAGTATGAGCAGTTCTTC 743 157-TL704-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCTCACCAACAGGGGGCGAAGATACGCAGTATTTT 744 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATCGGGTTAGCAGGAACACCGGGGAGCTGTTTTTT 745 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGCGAGCGGGCCCCAAGAGACCCAGTACTTC 746 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTTCGGCCGGGCTCAATCAGCCCCAGCATTTT 747 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCATAGGCGCTAGCGGTTACAATGAGCAGTTCTTC 748 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTCGGGACACGTCTATGGCTACACCTTC 749 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATTACTGCAATCCTACAATGAGCAGTTCTTC 750 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCGACTAGCGGGACCCTACGAGCAGTACTTC 751 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTTACAGGGGATGAACACTGAAGCTTTCTTT 752 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAATGCATATGATAATTCACCCCTCCACTTT 753 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTACGACCCACCCTAATCAGCCCCAGCATTTT 754 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCGGGACTTTCGCTACGAGCAGTACTTC 755 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGGTAGGGTATGGCTACAATGAGCAGTTCTTC 756 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGGACTGGACTACGAGCAGTACTTC 757 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGACTCGGGACGGTTCTCTGGGGCCAACGTCCTGACTTTC 758 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGGGCTACCACCGGGGAGCTGTTTTTT 759 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGAGCAAGGGACTTTCATTCCCCAGCATTTT 760 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCAACAGGGGGCGAAGATACGCAGTATTTT 761 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAAGCTACGAGCAGTACTTC 762 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCGTTCGGGGTACCTTCAGGGACCCAGTACTTC 763 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGACGGGGGGGCCCTACAATGAGCAGTTCTTC 764 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACACGACTAGCGGCACCGGGGAGCTGTTTTTT 765 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCATGGTAGCGGGAGGTACCTACGAGCAGTACTTC 766 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGACTGGGCCTTCTTACGCAGTATTTT 767 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCCTGGACAGGGGCGACTACGAGCAGTACTTC 768 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCAAGTCGCTTACTTGGCAGCCCGGGTAACACTGAAGCTTTCTTT 769 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCCCCCGGGGGGCGGACAGGACCTATAA CTATGGCTACACCTTC 770 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACGAGGTGGGACAGGGAGCCCGAAGGGTACGAGCAGTACTTC 771 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTTGGGGGATTACCTCCTACGAGCAGTACTTC 772 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCGAAGTTAGCGGGGGGACCCAAGAGACCCAGTACTTC 773 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCGGGACTAGCGGGTTCACAGATACGCAGTATTTT 774 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGGGGCGCGAGTGGAAAAAGAAAAACTGTTTTTT 775 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCCCACTAGCGGCCAAGAGACCCAGTACTTC 776 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCGTACGGGGACGCTTTGGGAGAGACCCAGTACTTC 777 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGATTGGGGGACTAGCGGGGGGTCTTGGAGTGA GCAGTTCTTC 778 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGGGAAACGGACTAGTTGGCCTCGAGAGACCCAGTACTTC 779 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTGGACTAGCGGGGGGGCCAATGAGCAGTTCTTC 780 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCCGGGGACAGGTTCTGAACACCGGGGAGCTGTTTTTT 781 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTACGGCGGGGGCCATGAGCAGTTCTTC 782 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCCCCGGGAGGGAATACTATGGCTACACCTTC 783 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTACCGACAGGGGACTAGCGGGGGTAGCG GACGAGCAGTACTTC 784 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCGCCGCAAGATACGCAGTATTTT 785 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTACATGGGACCAACACAGATACGCAGTATTTT 786 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCATAGACGGGAGCGAGACCCAGTACTTC 787 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATGGGGACGTGGGAAGACAATGAGCAGTTCTTC 788 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATCGTGCGGGACAGGGGACAGAGACCCAGTACTTC 789 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCCCGTCCGTTGGGACAAATCTACGAGCAGTACTTC 790 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTGGGACAGCTCCTACGAGCAGTACTTC 791 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCGGGACAGCTTGGGAGACCCAGTACTTC 792 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTTATGGGCAGAGTACCTACGAGCAGTACTTC 793 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAGGAACCTCCGGACGATGGTCTTTACGAGCAGTACTTC 794 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCTTAGCGGGGGCCTACTACAATGAGCAGTTCTTC 795 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGAGGGCGGATTGAACACTGAAGCTTTCTTT 796 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCACGAAGGGACCGATTCCACCTACTATAATTCACC CCTCCACTTT 797 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAAATCCGGGGGCGAGTTTACGAGCAGTACTTC 798 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTGGGGACAGGGGACAATGAGCAGTTCTTC 799 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGCCAGGGCTAGCAATCAGCCCCAGCATTTT 800 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAAAGGGACGGGCAGGGACAACATTCAGTACTTC 801 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCACCAAGGTGCCCCGGCAAGTTCTTACGGCTACACCTTC 802 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTTCGGCGGGGCTCAATCAGCCCCAGCATTTT 803 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTTCCCGGACGGGACCGGGGAGCTGTTTTTT 804 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGACAAGCGGGGGTTAATGAGCAGTTCTTC 805 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCACCGTTAGGGCATTTGAGCGTCGATGAGCAGTTCTTC 806 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTCGCGGGGGGAGAGCAGTTCTTC 807 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTACGTGGGGCGAAAAACTGTTTTTT 808 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAAAGGGGGCGGCAATGAGCAGTTCTTC 809 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCCCGCCTAGCCCTGACCGGGGAGCTGTTTTTT 810 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGGGTAAGAACTGAAGCTTTCTTT 811 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTCAATCGGGACCCCGACTACAATGAGCAGTTCTTC 812 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCCCCGGACTAGCGGGAGCGTACGAGCAGTACTTC 813 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCGCGGTAACGACCGCGCAGGGGGAGACCCAGTACTTC 814 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCCCGCGCGAAAGAGCGGTGAACACCGGGGAGCTGTTTTTT 815 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGGTAGTGGCGGGAGTGAGGAATGAGCAGTTCTTC 816 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGAGTCGATATCACCGCTCAATGGCTACACCTTC 817 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATCCGGGACTGAAGCTTTCTTT 818 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGAGGACAGCTACAAGAGACCCAGTACTTC 819 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCACCCTGAGTGCGGGAGTGATGCCAGATACGCAGTATTTT 820 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGGGACTAGCGGGAGGACCGGGGAGCTGTTTTTT 821 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCACCCCGGGACAGGGGTGGGTACACCTCCTACAA TGAGCAGTTCTTC 822 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGACCGACTAGCTGGGGAGCAGTACTTC 823 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGTAGCGACTAGCGATGAGCAGTTCTTC 824 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTAGCCTGAGGGTCTCCTACGAGCAGTACTTC 825 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAGCCGGACAGGGCTTTTCATCAGATACGCAGTATTTT 826 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCAGGGAGAGGCACTGAAGCTTTCTTT 827 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCTATGGACTAGCGGAGCGATTCAGGATACGCAGTATTTT 828 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCATTTTGGGGGAGGGGTTTGGTCCTACGAGCAGTACTTC 829 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTAGGACAGGGTATTGACACCGGGGAGCTGTTTTTT 830 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTACCGGGACTGATACCTACGAGCAGTACTTC 831 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTTCGCGACACTTCTCCTACGAGCAGTACTTC 832 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGACCTGCTACTAGCGGGTTGGGGGATGAGCAGTTCTTC 833 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGGGGACAGGGTGGAGACCCAGTACTTC 834 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCTAGCGGTCCCACAGATACGCAGTATTTT 835 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATAGGGGGGGGGGATACGAGCAGTACTTC 836 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGGGGCAGCTCCTCAGGGGATGGCTACACCTTC 837 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGGAAGTACAGCAGACTACGAGCAGTACTTC 838 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCGGGACAGGGGGTAAAAATGAAAAACTGTTTTTT 839 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGAGGCGGGTCAAGCACAGATACGCAGTATTTT 840 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAGCGAGAGGATAGCGGGAGGGCGACAAGAGACCCAG TACTTC 841 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCCCTAGCGCCGAGCAGTACTTC 842 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCGGGAGGGCTTGAAGATGAGCAGTTCTTC 843 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATTTACAGGGGATGAACACTGAAGCTTTCTTT 844 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAACCGGACAGATAGCTCCTACAATGAGCAGTTCTTC 845 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAGGGGCTAGGTCGGGTGAGCAGTACTTC 846 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGATCTACCGGGACAGGGATACGAGCAGTACTTC 847 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTACCCCGACAGGGTTAAACACTGAAGCTTTCTTT 848 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCATGACGGGAGCCGGTAACTATGGCTACACCTTC 849 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGAGCGAACCTTTACAGGGAATGGGG GCCTATGGCTACACCTTC 850 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGCCAAGGGTGAACTTCATACGAACACC GGGGAGCTGTTTTTT 851 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCCTGGGACTAGCGGACACAGATACGCAGTATTTT 852 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTAACAGGGGGGGGGAATCAGCCCCAGCATTTT 853 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAACTTAAGGGACAGGGGGTTGACTATGGCTACACCTTC 854 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCCAGACAGGGCTCACAGATACGCAGTATTTT 855 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAAGAAGGCGGGCCGCCGCCAAGCTCCTACAATGAG CAGTTCTTC 856 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGAGGTCGGGGGAGGGGGACGAGCGAACACCG GGGAGCTGTTTTTT 857 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGATGGAGGGGACGCACATACCCAAGAGACCCAGTACTTC 858 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGAATTAGCGGGACCCACACAGATACGCAGTATTTT 859 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCGCCAAAGCCCATGGCTACACCTTC 860 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCACAGACGGGACTCGACGAGACCCAGTACTTC 861 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTCGACAGTAACACTGAAGCTTTCTTT 862 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGCAGGGTTACACCGGGGAGCTGTTTTTT 863 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTACAGGAGACCTCCTACGAGCAGTACTTC 864 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGGTAATGAGCAGTTCTTC 865 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGAGGGGGGGGAAGAGACCCAGTACTTC 866 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAGGTTGTAGGAGGGCCCGGGGAGCTGTTTTTT 867 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATTCGGGCAGGGGTTACGAGCAGTACTTC 868 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCCATTTTAAGGACGAGGAGCACACTGAAGCTTTCTTT 869 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGAATGGATAGATACGCAGTATTTT 870 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGATGATAGGGCCGGGGAGCTGTTTTTT 871 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAACCGGACAGTCCTACAATGAGCAGTTCTTC 872 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTTAGACTAGCCTACAATGAGCAGTTCTTC 873 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGACAGGGATACAGCCCCAGCATTTT 874 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGGGACCCTCGGGACAAGTAGTACATACTATGGCTACACCTTC 875 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCTTTTTTTGGAGTGTTAGCAGATACGCAGTATTTT 876 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTAACGGGGACACTGAAGCTTTCTTT 877 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGACAGGGATCTCAGCACCGGGGAGCTGTTTTTT 878 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAATCCACAGGGGAGTGAGCAGTTCTTC 879 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAACAGGGACAGCTACCTACGAGCAGTACTTC 880 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGTCCGGGACAGGGCTTCCGGGGGGGAGACCCAGTACTTC 881 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCGAACGACGGGATCTCTGGAAACACCATATATTTT 882 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGGACTAGCGGGATACAAGAGACCCAGTACTTC 883 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCAACCGGGACAGAAGAGACCCAGTACTTC 884 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCGGGACTAGACCGATAGAGATGTTGAAC GAGCAGTACTTC 885 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGAGTCCGGGGACTACCTGGTTGCAGTACTTC 886 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGGACTAGCGGGGGTTGAAAATGAGCAGTTCTTC 887 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCGCCACTAGGTGATGAGCAGTTCTTC 888 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGAATTCTCCTTCCAAGAGACCCAGTACTTC 889 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGGGGACTAGCGGGATCTACAATGAGCAGTTCTTC 890 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAATATTCATTAGGGCAGGGAGGCGACGAGCAGTACTTC 891 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGGCCCCGGACAGGGCAATGAGCAGTTCTTC 892 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGGGGCCAGTCCTACGAGCAGTACTTC 893 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGACCAGCGTACTATGGCTACACCTTC 894 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAACGCGCGCGGCCTTTCGGGACAGGGGCCCACCGGGG AGCTGTTTTTT 895 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCGGGACCCACGGGACTAGCGATCCACTACGAGC AGTACTTC 896 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCATCACTAGCGGGGGGCCGAGGGGAGCAGTTCTTC 897 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGAGTCGGCCCCCGGGTGGACAGGGAACACTGAAGCT TTCTTT 898 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTCGGGACAGCTCGAACACTGAAGCTTTCTTT 899 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCACAGGGTACGAGGAAGAGACCCAGTACTTC 900 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCCGGACCTGATATAGCCAAAAACATTCAGTACTTC 901 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTTTAGAGCGGACTAGCGTGGTCACAGATACGCAGTATTTT 902 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTTTCGGACGGGAGGACGGATACGCAGTATTTT 903 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGTCACCCGGGACAGGGCTCAAGAGACCCAGTACTTC 904 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGCGACTAGCGGGGGCACCGGAGAGACCCAGTACTTC 905 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGACCGTTACCGATCACCCCTCCACTTT 906 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCGTGGAGGCCACTGAAGCTTTCTTT 907 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAAGATACGGCGCACGAGCAGTACTTC 908 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTAGCGGGGAACCCTACGAGCAGTACTTC 909 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGTAGCTGGCACAGATACGCAGTATTTT 910 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGACGCCCCCGGGCCAATGAACACTGAAGCTTTCTTT 911 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAATTCCGGGTTTGGAATTCACCCCTCCACTTT 912 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCGGAGGGACTAGCGGGAGGGCCGGGGGGACCGGGGAG CTGTTTTTT 913 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCATTGGCTATCGACGAGCAGTACTTC 914 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATCGGCTAGCGGGAAAAAGGGGGGCA GATACGCAGTATTTT 915 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGCCCGGGACAGCGCCTACGAGCAGTACTTC 916 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCCCCGCTTGAGGAGCAGTACTTC 917 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATTTGTCTGGGGCCAACGTCCTGACTTTC 918 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGACAGGCCTCTCCTACGAGCAGTACTTC 919 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCATTCCACTAGCGTCAGCCTCCTACAATGAGCAGTTCTTC 920 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTACTACCCGGTCAGCCTGACAATGAGCAGTTCTTC 921 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTGGAGGGGAAACGCAGTATTTT 922 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGCTGCCGATTCCGAGCAGTACTTC 923 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGAGGGTCTGGGGTTCGAGCAGTACTTC 924 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTAGTCGGACTAGCGGACACCTCCTACGAGCAGTACTTC 925 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCACTCCCGGACCGGGACAGAGCTCCTACGAGCAGTACTTC 926 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATCGGGGGGCCCAAGAGACCCAGTACTTC 927 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGCCCCGGGACAGCCAGACAATGAGCAGTTCTTC 928 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCCGAATTGGACAGGGGTGACTATGGCTACACCTTC 929 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGATGTCGGGAACATTCAGTACTTC 930 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTAGGTGCAGGGAGGTATGTTGAGCAGTTCTTC 931 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTACGGCTGTCGGGAGATACGCAGTATTTT 932 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGATCGACAGGAGATCTCTGGAAACACCATATATTTT 933 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTCGGGGGATCGAGGACAATGAGCAGTTCTTC 934 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCAGGGATCAACGAGCAGTACTTC 935 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCATTGTCTAGTAGCCACAATGAGCAGTTCTTC 936 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATCCGGCAAGCAACTAATGAAAAACTGTTTTTT 937 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTGAGCTGGACACCAGCTCCTACGAGCAGTACTTC 938 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGGGACAGGGCCTACAATGAGCAGTTCTTC 939 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTGGGGACTAGCGGGAGGCCCGAACACCGGGGAG CTGTTTTTT 940 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGCAGGAGGACGGGAGGAGGCAATTCACCCCTCCACTTT 941 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGAGGGGGATAATCAGCCCCAGCATTTT 942 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCGACGGACTAGCGGGGCGTCCTCCAGAGACCCAGTACTTC 943 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGAGCGGGGGCCACAGATACGCAGTATTTT 944 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCGGGACAGGGGCTTTCCAATGAGCAGTTCTTC 945 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGGGTTACGGAGGTGCAGGGCTGTTTTTT 946 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGTAGGGGACGGGACTAGCGTTTACAAT GAGCAGTTCTTC 947 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCAGACAGGAAACTACGAGCAGTACTTC 948 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGAAGGGGGACTAACGTCAGATACGCAGTATTTT 949 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAACTACGGCTTGGGGAGCTGTTTTTT 950 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATAGGGCGGGAACGGAGACCCAGTACTTC 951 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCTCAGGAGGACTCCTATAATTCACCCCTCCACTTT 952 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGGGGGGGACAACTCCTACTACGAGCAGTACTTC 953 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGATTTAAGCGGGTGGAACACCGGGGAGCTGTTTTTT 954 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCGCTCAGGGATCTATAATTCACCCCTCCACTTT 955 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGGGGGGATAGCGGGAGAGAATGAGCAGTTCTTC 956 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCCACGAATGCAGGGGGCGGTCAATTGGGGGAGCAGTACTTC 957 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGATGCCCGGGACAGGGTTGAAGAGACCCAGTACTTC 958 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGCTACGGCACAGATACGCAGTATTTT 959 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGTCCGCGGGACACCTAGACGCTACGAGCAGTACTTC 960 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCGCGGGGAGCTCCTACAATGAGCAGTTCTTC 961 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGTGGATCTCAACACTGAAGCTTTCTTT 962 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATCTACGGGGGGGAAACTACGAGCAGTACTTC 963 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTACAGGGGGCTGGGAACACTGAAGCTTTCTTT 964 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATCCAGGTGGTGTGGTCTACAATGAGCAGTTCTTC 965 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAAACACAGGGATATTTACCGGGGAGCTGTTTTTT 966 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGAAATTGCCACTGAAGCTTTCTTT 967 158-TL704-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGATCGGGAGGAAGTTCTTC 968 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCAACGACATGACACCTGGGTGGATCTTC 969 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCTCTGACTCCCGGGAGGGGGAGCGAGGAACTGAAGCTTTCTTT 970 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATAGACGGCACTTCCTACGAGCAGTACTTC 971 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCTTTACTAGCGGGAACACCGGGGAGCTGTTTTTT 972 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCATCTTCTACCGGGGGGGCTAACGGGGAGCTGTTTTTT 973 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCATAGGGGGGGGGACAGATACGCAGTATTTT 974 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAAGAAAACTACTCTGGAAACACCATATATTTT 975 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTAACGGGGCCGAACACTGAAGCTTTCTTT 976 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGACGGGGACCGGAGTGGAGCAGTACTTC 977 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCAGGAGGGGGGGGAGCCTACGAGCAGTACTTC 978 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGATCTGGCAGGGTTCGCCTACGAGCAGTACTTC 979 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGCCGGGACCTACGAGCAGTACTTC 980 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTTGGGACAGCCTACAATGAGCAGTTCTTC 981 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGAACCGGGACTAGCGGGGGTCTTGAGCAGTTCTTC 982 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCCGCCGGGGGCGGGGAGCTGTTTTTT 983 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGAACAGGGGGTATACTATGGCTACACCTTC 984 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAATAGAGACACCGCCTCAAATGAGCAGTTCTTC 985 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTCCATTTGGGACCAATGAGCAGTTCTTC 986 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGACCGGGCCCCAACACCGGGGAGCTGTTTTTT 987 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGGGGAGAACTATGGCTACACCTTC 988 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGTGAGGACGGTATGAACACTGAAGCTTTCTTT 989 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGAACGGGTTATCCCAATGGCTACACCTTC 990 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGACGGAGCCCGCTACAATGAGCAGTTCTTC 991 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGACAGGGTTACAAGAGACCCAGTACTTC 992 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGGATGGACTAGCGGGGCGACGCAGTATTTT 993 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAGGGACAGGGGGACGAGCAGTACTTC 994 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCGAGTCTGGGGACTAATGAAAAACTGTTTTTT 995 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAAACGGCGCTTGGCGGGCGAACTACAATGAGCAG TTCTTC 996 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCCGATAGGGACAGGGGAAAACATTCAGTACTTC 997 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCTTTCGGGCGGGGGGGACAATGAGCAGTTCTTC 998 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTACACAGATACGCAGTATTTT 999 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCAGTGACAGGGGGCTTGACACTGAAGCTTTCTTT 1000 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCATCCGGGGGGGCAGGAGCCTACGAGCAGTACTTC 1001 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTCCCCCTCGGGCAGAACACTGAAGCTTTCTTT 1002 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGACAGCCAATTCACAGATACGCAGTATTTT 1003 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTTGGCGGACCGGGACAGGAGAGAGAAACACTGAAGCTTTCTTT 1004 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTCCGACAGCTCCTATAATTCACCCCTCCACTTT 1005 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGAAAGCGGGAGTTACTACGAGCAGTACTTC 1006 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCTGGCTCTAGCGGGGCCGACGAGCAGTACTTC 1007 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCGCACTAGCGGCCCGTACAATGAGCAGTTCTTC 1008 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCGGGGGAGGGGGCTGGTACAATGAGCAGTTCTTC 1009 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCTGGGACTAGCGGAGCCTACAATGAGCAGTTCTTC 1010 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGTGCTGGACAATGAGCAGTTCTTC 1011 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACGGGACAGCCAACAATGAGCAGTTCTTC 1012 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCCCCCGATACCTACAATGAGCAGTTCTTC 1013 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGGGACTGGGGCGATGTGGTACTTC 1014 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGAGACAGGGAGACTACGAGCAGTACTTC 1015 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCCGAGGGTAGCGGACTCTACGAGCAGTACTTC 1016 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTGGGAAGGCTCCTACGAGCAGTACTTC 1017 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCCGCCTCGCTTCGACTAGCGGGGGGTTGGAATGAGCAGTTCTTC 1018 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCAAAGCCGAAAAGCAAAGGGACAGGGTTCCCTGGGAGCAG TACTTC 1019 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGACCCTCGGGGGGGGTGGAGACCCAGTACTTC 1020 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGATCGGGGGGGGGCAAGGGAGCAGTACTTC 1021 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGACTCCTAGCACAGATACGCAGTATTTT 1022 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTTGGCGGGGGGGCCAATGAGCAGTTCTTC 1023 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAACGGGAGGGACAGGGGGCACTGAAGCTTTCTTT 1024 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGATGTGGCAGGGGAGGGGCAGGAGCAGTTCTTC 1025 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCTTTATAACGGGGAGCTGTTTTTT 1026 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCCTCAGGGTGGGAGCAGTACTTC 1027 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGGGGAATGAAGCTTTCTTT 1028 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGATTCAGGGATGAACAATGAGCAGTTCTTC 1029 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCATCTTCGGGGGACGGGGGTAAAGATGAGCAGTTCTTC 1030 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTAATTTCCAGGGGCACTACGAGCAGTACTTC 1031 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTCGGGGGGGACTACAATGAGCAGTTCTTC 1032 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTGGACAGGGAATGAGCAGTTCTTC 1033 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCGGTACTGGGGCGGCGTGGAAACACCATATATTTT 1034 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCCGGGAGTCCGTCCGGGGAGCTGTTTTTT 1035 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGATAGGAAGAGGACAGGGCCCTTGAACACTGAAGC TTTCTTT 1036 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTTTTGCAGTCCTACAATGAGCAGTTCTTC 1037 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGAATCTACCCTTTCTGGCACGGACACAGAT ACGCAGTATTTT 1038 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCTCCAGAGGGTGGGCACTGAAGCTTTCTTT 1039 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCCCGGACGGGCTCCTACGAGCAGTACTTC 1040 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCCAACCAAGATTTAACTTATTCGCTAACTATGGCTACACCTTC 1041 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCGGGACAGTATACACCGGGGAGCTGTTTTTT 1042 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCCCCGGGCGGGGGAGAGCAGTACTTC 1043 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTGGGCTATGGCTACACCTTC 1044 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGAATGGACAGGGGGCAGAGATACGCAGTATTTT 1045 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGGCCATTGGGGGAGATGGCTACACCTTC 1046 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCCAAGAGGGCTTAAGCTACGAGCAGTACTTC 1047 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGTCCCGGACAGGGGGCGACAGATACGCAGTATTTT 1048 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGGGACAGGGAACTACGAGCAGTACTTC 1049 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCTTATCTTGGCTCCTACAATGAGCAGTTCTTC 1050 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACGTGGGGGCAGGCCCGCAGTATTTT 1051 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAGTCAACGCTCCACAATGAGCAGTTCTTC 1052 172-TL720-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATAGCCGGGCTAGCGGGGGGCCTTAATGAGCAGTTCTTC 1053 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCTGACTCCCGGGAGGGGGAGCGAGGAACTGAAGCTTTCTTT 1054 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCCGATAGGGACAGGGGAAAACATTCAGTACTTC 1055 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATAGACGGCACTTCCTACGAGCAGTACTTC 1056 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAGGGCAACTTGCACCCGGGGAGCTGTTTTTT 1057 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGCCGGGACTAGCGAGTCCAATGAGCAGTTCTTC 1058 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACGTGGGGGCAGGCCCGCAGTATTTT 1059 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCGGGACAGTATACACCGGGGAGCTGTTTTTT 1060 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCTTTACTAGCGGGAACACCGGGGAGCTGTTTTTT 1061 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTTCGGGCCCCGTGGAGGACATTCAGTACTTC 1062 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTCTTGAGGCCAACGTCCTGACTTTC 1063 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTACGGGACAGCTGAACACTGAAGCTTTCTTT 1064 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAGTCAACGCTCCACAATGAGCAGTTCTTC 1065 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGACGGGGACCGGAGTGGAGCAGTACTTC 1066 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATGGGGTGGAGTCAGCCCCAGCATTTT 1067 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCGACGAGCGGGATACGCAGTATTTT 1068 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGTGCTGGACAATGAGCAGTTCTTC 1069 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCCAGTCGGGGGGTTCGAACACCGGGGAGCTGTTTTTT 1070 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCCTGGGGTTCGCGCGGCTACACCTTC 1071 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTGGGGACAGGGGACGGCTACACCTTC 1072 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACGGGACAGCCAACAATGAGCAGTTCTTC 1073 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCCAAGAGGGCTTAAGCTACGAGCAGTACTTC 1074 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAATAGAGACACCGCCTCAAATGAGCAGTTCTTC 1075 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGTCCTCGCCGGGGGCTCCCTACGAGCAGTACTTC 1076 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAACGGGAGGGACAGGGGGCACTGAAGCTTTCTTT 1077 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCACCACAGATACGCAGTATTTT 1078 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTAATAGGGGGAGCGAGCAGTACTTC 1079 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCTCCCCAGCGGGGGTCCACAATGAGCAGTTCTTC 1080 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCTCCCCTAACAGTTCTCACCGGGGAGCTGTTTTTT 1081 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTTGGGGGGCTGGCCACTGAAGCCTTCTTT 1082 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTGGCGGGGGGGCCAATGAGCAGTTCTTC 1083 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTACCGCCGAGCGCCGGCTACAATGAGCAGTTCTTC 1084 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGTTAACAGGGTCTAACAATGAGCAGTTCTTC 1085 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCGGCTTTCCGGATACGCAGTATTTT 1086 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCGCCGATGTGCCCGAAACCTCACGGGACAGGGTC CGTAATGAGCAGTTCTTC 1087 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGATGGCTCCGGGACAGCCCCCTACGAGCAGTACTTC 1088 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCAGGCGGAGAGCAGTACTTC 1089 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGATACAGGCCTCTCTGGAAACACCATATATTTT 1090 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCGGGACTCTTCCTACAATGAGCAGTTCTTC 1091 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGGCGGGGGAGCAGTTCTTC 1092 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCGGACCCCTACGGAGTGATATACGAGCAGTACTTC 1093 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGATATCAGCGGGGGCCCCTCCTCCTTC 1094 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTGACAGTTCGGAAGCTTTCTTT 1095 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGAGAGGGACAGCGGGACTAACTATGGCTACACCTTC 1096 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTCACCCCGGGTAGTACAGATACGCAGTATTTT 1097 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGAGGACAGGGTTTGTCACTGAAGCTTTCTTT 1098 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTCGCACGTGGAGCCAAGGGTGGCTACACCTTC 1099 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCCCCCTCGGAGGGCAGTCCTACGAGCAGTACTTC 1100 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATCGCGATTGGGGCGGAGCAGTACTTC 1101 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGACTAGGGACAGGGGGGAGCAGTTCTTC 1102 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGAGGATTGGCTAGCGGGGGGGCCT GCAGATACGCAGTATTTT 1103 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCCATGACAGCTACGAGCAGTACTTC 1104 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTCGACACTAACTACTATGGCTACACCTTC 1105 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTACGGGCTTAAACCACACTGAAGCTTTCTTT 1106 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCGGGGATCCAAAACACCATATATTTT 1107 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTGAGGACGGTATGAACACTGAAGCTTTCTTT 1108 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTGGACGGGTTACGGGGGAGACCCAGTACTTC 1109 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGGCCCTTTACCCTCCTTC 1110 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAGCCGGGACAGGGACCGTAATGAGCAGTTCTTC 1111 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCTCTGTACAGCGGGATAGAGAGCTCCTACAAT GAGCAGTTCTTC 1112 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGTGGGACAGGGTTCCAATGAGCAGTTCTTC 1113 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATCCCGACCCGCTGGATGGCTACACCTTC 1114 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTAGCGGGGGGGAAAATGAGCAGTTCTTC 1115 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATCCAGAAATCAACACTGAAGCTTTCTTT 1116 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTCCAGACAGGGACAATGAAAAACTGTTTTTT 1117 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAACCTCTGGGGGGGCTCCTACAATGAGCAGTTCTTC 1118 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGACAGCCAATTCACAGATACGCAGTATTTT 1119 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGAAAGCGGGAGTTACTACGAGCAGTACTTC 1120 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGGAGACTAGCGGGGACCGACAATGAGCAGTTCTTC 1121 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGACTCCCGGTACAATGAGCAGTTCTTC 1122 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTGATCCCCGGGGCCACAGATACGCAGTATTTT 1123 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAGGACAGATCCTAACCCTGGAAACACCATATATTTT 1124 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATAGCGACACCAGGGGAGCAGTACTTC 1125 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCCCCGGAGGAGGAGTCGATGAAGCTTTCTTT 1126 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGATCTCTGGACAATGAACACCGGGGAGCTGTTTTTT 1127 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTTGGGGGGCTGGCCACTGAAGCTTTCTTT 1128 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGGGGCTAGGGCGGGGGGGCTTGAACACCGGG GAGCTGTTTTTT 1129 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGACCGGACCGGCGAGACCCAGTACTTC 1130 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGCAGGGGGGACTAGCGGGGGGATTGAGCAGTTCTTC 1131 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGGTAGCGGGCAAGAGACCCAGTACTTC 1132 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGGGGGACAGAACTATGGCTACACCTTC 1133 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGGTTATCCTCCTACGAGCAGTACTTC 1134 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGACCACTAGCGAGGGTAAACTACGAGCAGTACTTC 1135 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTGGACAGTTTCGTCGGACTATGGCTACACCTTC 1136 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTATTTCTCTTCGGTAAGCCCCAGCATTTT 1137 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGACGGGCCTCTGGATGAGCAGTTCTTC 1138 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTACTGGGACAGGGCTTACTTC 1139 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCCGACAGGGGAAGACCCAGTACTTC 1140 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCCCAGGAGGTGATGGCAATCAGCCCCAGCATTTT 1141 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGAATCTACCCTTTCTGGCACGGACACAGAT ACGCAGTATTTT 1142 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCTCCAGAGGGTGGGCACTGAAGCTTTCTTT 1143 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTTCACCGACAGGGGGCCCCTACAATGAGCAGTTCTTC 1144 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGTGCCTCCCGGGGGCCCAGATACGCAGTATTTT 1145 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGAATGGACAGGGGGCAGAGATACGCAGTATTTT 1146 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGTAGCCAATGAGCAGTTCTTC 1147 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGCCGCCCGTGGACCGGGGAGAGACCCAGTACTTC 1148 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGCTCCCCGGACATATAGAAACAGATACGCAGTATTTT 1149 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCGACCTTAACTATGGCTACACCTTC 1150 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCACAAACAGGGAACACCGGGGAGCTGTTTTTT 1151 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTACCAGGGTACCCCGGAAACACCATATATTTT 1152 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTCCGCGCCGGGACCCTGGGGGTGAGCAGTTCTTC 1153 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCGATCCTTGTTGCACAGGGTCATGAACACTGAA GCTTTCTTT 1154 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATTGGAGTGGCATCCCCGGGGAGCTGTTTTTT 1155 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCCGCTGAACTGACTGGGTGGAGCGGGGGGCCC AATGAGCAGTTCTTC 1156 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCACCAAATGGTCTAACTATGGCTACACCTTC 1157 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCGACAGGGCGGAAACTATGGCTACACCTTC 1158 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGCAGCGGGGGGGGACCGGGAATGAGCAGTTCTTC 1159 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGAGGCCGGTTATAATCAGCCCCAGCATTTT 1160 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTCGGGACAGGATAATTCACCCCTCCACTTT 1161 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGCGAGACAGGGAAAGGAGACCCAGTACTTC 1162 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAACAAAACACCGGGGAGCTGTTTTTT 1163 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTGGGAACTGACAGGCCCTCCCTACGAGCAGTACTTC 1164 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGGGACAGGGGTGAATGAGCAGTTCTTC 1165 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGGGCAGTTCTATGGCTACACCTTC 1166 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAACGGGACAGGCCTCCGGGCTGGGGGCTACACCTTC 1167 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATCCCGACATCGGGGAGCTGTTTTTT 1168 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCCCCGATACCTACAATGAGCAGTTCTTC 1169 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGTTGGATCCAATGAGCAGTTCTTC 1170 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAATGGTCCACACTGAAGCTTTCTTT 1171 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCGATAGATACGCAGTATTTT 1172 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGCTCCTGAGGCTAGCGGATACAATGAGCAGTTCTTC 1173 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGGGGAGACCCTACACACTGAAGCTTTCTTT 1174 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAAACTAGCGGGGGGGGGAGATACGCAGTATTTT 1175 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTATGGACAGAACTATGGCTACACCTTC 1176 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGAGGGTGTTAGCACAGATACGCAGTATTTT 1177 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATCTAAGGGGCAATGAGCAGTTCTTC 1178 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGAGGGGGGACAGCTGAATGAAAAACTGTTTTTT 1179 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGACAGCCTACGAGCAGTACTTC 1180 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTTCAGGGGACGAGGGTCAGCCCCAGCATTTT 1181 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCCTTGGACGACCCCACCGGGGAGCTGTTTTTT 1182 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTACACCAGGGTCCCTCCTACGAGCAGTACTTC 1183 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTCCGGGTAGGGAGAACACCGGGGAGCTGTTTTTT 1184 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTACAACACCAAACTATGGCTACACCTTC 1185 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCAACATACACAGCACAGATACGCAGTATTTT 1186 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGAAAGACTGGGGTCTCCACTGAAGCTTTCTTT 1187 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGAATCMCTTTTCCCTGGACACCGGGGAGCTGTTTTTT 1188 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAGGGGGACAGAACTATGGCTACACCTTC 1189 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGAGGGGCAGGGGGCTGAGTGAGCAGTTCTTC 1190 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGTATGCAGGTCCTAACTATGGCTACACCTTC 1191 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTCTGACTAGCGGGGATGAGCAGTTCTTC 1192 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCGGGACAGGGACCCCAGATACGCAGTATTTT 1193 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCGCCCATACTCCAAAGAGACCCAGTACTTC 1194 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGTACTCCTCTGGGGCCAACGTCCTGACTTTC 1195 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGTTGGGCGGGGAGATCTACAATGAGCAGTTCTTC 1196 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCGGACTAGCGGGGGGGCGGATGAGCAGTTCTTC 1197 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCGGGACTAGCGGCGACAATGAGCAGTTCTTC 1198 173-TL720-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAACGACATGACACCTGGGTGGATCTTC 1199 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTAGGGGACTAGCGGGAGTCAATGAGCAGTTCTTC 1200 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTTCCGTCGCGGGAGGAGACCCAGTACTTC 1201 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACGGCCCCGAGCGGGCTGAAGCTTTCTTT 1202 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGATTGGGAGAGCACAGATACGCAGTATTTT 1203 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAATCCGGGTGGGGGGAAATCAGCCCCAGCATTTT 1204 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAAGAGGGATACGCCTACGAGCAGTACTTC 1205 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGATCAATGAGCAGTTCTTC 1206 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTAAGCGGGAGGGCACCGGGGAGCTGTTTTTT 1207 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTAAGACTCCCCGGGCACACTGAAGCTTTCTTT 1208 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGCCAGGGGTACCGACGAGCAGTACTTC 1209 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCTCCATAGGAGGAGACGAGCAGTACTTC 1210 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCTGACAGTATTCAACGGGTGTTC 1211 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCCCACAATGAGCAGTTCTTC 1212 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCCTTTTCGGGGTTGGGCTACGAGCAGTACTTC 1213 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGACTGACAGGGGAAAGGACCTACGAGCAGTACTTC 1214 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCGCCCTCCCCGGGTCCTCCTACGAGCAGTACTTC 1215 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCCGATTTCTTAGCGGGAGTCTTGATGAGCAGTTCTTC 1216 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGCACCTACAGGGCACCCCCTGGAAACACCATATATTTT 1217 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAATCTCTGAAGCTTTCTTT 1218 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCCCCGACAGGGGGTGGACACTGAAGCTTTCTTT 1219 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGACATTTGAAGCTTTCTTT 1220 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCCGACACGGCCCAGCATTTT 1221 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATAGGCTGGGGTAGCTCCTACAATGAGCAGTTCTTC 1222 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAGGGACAACTAGCTCCCGGGGAGCTGTTTTTT 1223 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGGAGGGGATGGCTGAAGCTTTCTTT 1224 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAAGACGGACATGAACACTGAAGCTTTCTTT 1225 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTTGAGGGGCGACTTACTGAAGCTTTCTTT 1226 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCACCCAACCGGAGACTGTTTTTT 1227 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCACCAGCCCAGGGGGCCGGGGCTACACCTTC 1228 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGAAGGCCGGACCTCCAGCTCCTACAATGAGCAGTTCTTC 1229 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGGGGGGAACACTGAAGCTTTCTTT 1230 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTTACGGGACAGGGGGCGGCTATGGCTACACCTTC 1231 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACCTTAGAGTTTTCCTACGAGCAGTACTTC 1232 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAACTAGCGGGCCATACAATGAGCAGTTCTTC 1233 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTCGTCGCGGGCACAGATACGCAGTATTTT 1234 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGTCGAAACGAGCAGTACTTC 1235 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTAACGGGGACAGGGACCAAAAACATTCAGTACTTC 1236 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGTCCAAGGGGGTTCCTACGAGCAGTACTTC 1237 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGAATCCCAAGCAGGTTCCTACAATGAGCAGTTCTTC 1238 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTAAGTCTAGCTGGGGATGAGCAGTTCTTC 1239 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGACGGACAGGGGCGCCAGCACTGAAGCTTTCTTT 1240 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTGGGTCCGGGGACTAGCGTCTACGAGCAGTACTTC 1241 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACGGGGCAGGGGACTACGAGCAGTACTTC 1242 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCCCCGGGACGGAGGGCGAGCAGTACTTC 1243 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAGCCTGTTACGGGACTAGCGGGGCGG AGCTCCTACAATGAGCAGTTCTTC 1244 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCGGACAGGATGAACACTGAAGCTTTCTTT 1245 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGAAGCCGACCAGGGGGTATACAATGAGCAGTTCTTC 1246 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGATAAGAGGGACGAGCAGTACTTC 1247 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTAATTGCACTAGGTATGGAAGATACGCAGTATTTT 1248 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCTGGAGTGGTGACAGCAGTAACACTGAAGCTTTCTTT 1249 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTTGGAGGGGACGGCTACGAGCAGTACTTC 1250 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCCCCAGCGGGACTCACAGATACGCAGTATTTT 1251 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCACTGACCCTCTTCCTAGCGGGGCCCTACAAT GAGCAGTTCTTC 1252 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCATGACAGGTCAACTAATGAAAAACTGTTTTTT 1253 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTGGGAGGGCCCCTACGAGCAGTACTTC 1254 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGGACAGGGTCGGGGAGCTGTTTTTT 1255 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATAGGACTGTCTAGTCTCAATGAGCAGTTCTTC 1256 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTTTGGGGACACTGAAGCTTTCTTT 1257 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTCAAGGGAGAGAATGAAAAACTGTTTTTT 1258 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGGGACAGGTTGGGGAGGCACTGAAGCTTTCTTT 1259 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGACCCAGGGGGGACAGGGCTTTTGGAAAAACTG TTTTTT 1260 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCTCAGGCCGGGACAGGGGTTACGAGCAGTACTTC 1261 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGCTCCGGGGGGGACAGGGGTGGTCGAGACCCAGTACTTC 1262 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAGACTGGGACAGGGAACACTGAAGCTTTCTTT 1263 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGATGTAGATACTGGAAACACCATATATTTT 1264 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGTTTAGGACCGAACACCGGGGAGCTGTTTTTT 1265 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCTCACCCTCATCTAGCGGGAATTGGGATGAGCAGTTCTTC 1266 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAAGGGGACAGGGTTTGAGGGGGGTCGCGGCTTTCTTT 1267 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGACTAGCGGGAGCCTTAAGGTTCGAGCAGTTCTTC 1268 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGGCAGGAGCTCTCTCCTACGAGCAGTACTTC 1269 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCCCACCGCTACGAGCAGTACTTC 1270 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGCCACATTCCCACAGGGGGCACCTTACAATGAGCAGTTCTTC 1271 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGGGAGCGACTAGCGGTTACCTACAATGAGCAG TTCTTC 1272 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTCCTTACGAGACGTGGGCGAAGATCGAGAACACTGAAGCT TTCTTT 1273 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTAGGGACAGATTACGAGCAGTACTTC 1274 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCACGTCGGGACTAGCGGTTACGAGCAGTACTTC 1275 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGTGACAGAGAACACTGAAGCTTTCTTT 1276 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCTCGGGGTATCAACATTCGAACACTGAAGCTTTCTTT 1277 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCATTCGGACCGGGGGTGCTGGCAATCAGCCCCAGCATTTT 1278 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGGAACTAGCCCCTACAATGAGCAGTTCTTC 1279 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCCCCCGACTAGCGGGAGGGGAGACCGGGGAGCTGTTTTTT 1280 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGCTGACAATGAGCAGTTCTTC 1281 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGGAGCGGGAGCCCCCGTTGAGCAGTTCTTC 1282 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTTCGGCCCCCTCCCTACGAGCAGTACTTC 1283 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTACAGATACGCGAGATGGCTACACCTTC 1284 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTACTCCTGCTAGCGGGAGGGAGTACAATGAGCAGTTCTTC 1285 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTACAGGGGGCGAGGGCGACCGAGCAGTACTTC 1286 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCAGAGATTGGGGGAGCTCCTACAATGAGCAGTTCTTC 1287 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCGTAGCGGGAGGGTTGTTGTATGAGCAGTTCTTC 1288 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGTGATGTAGCGGGAGGTTACGAGCAGTACTTC 1289 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAAGAGCAGGGCCGGCAGTCCCTACGAGCAGTACTTC 1290 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACGGCGGAAGGTTCGATGAGCAGTTCTTC 1291 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTACCCGGGACTAGCGGGAGCATACGAGCAGTACTTC 1292 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCAAGCGACCGCTCCTACGAGCAGTACTTC 1293 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCCGGACTGAAGCTTTCTTT 1294 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTATATGCCGCAAGTTCAGTAGCTAGCGGGGG GACAGATACGCAGTGTTTT 1295 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAGAGCTTACCCTCGACAGGGGGTCACAATGA GCAGTTCTTC 1296 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACGCCCCCGAGCGGGCTGAAGCTTTCTTT 1297 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTCAGAACTAGCAACGCGCAGTATTTT 1298 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCTAGCAGCCCCAGTGGAGTAGCGGGAGACGTGGAGACCCAGTACTTC 1299 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTCACCGGGACGGTAATGAAAAACTGTTTTTT 1300 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATGGATCGTGCTAGCACAGATACGCAGTATTTT 1301 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCCATAATTTCACAGGGGATGAGACCCAGTACTTC 1302 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTACGACTAGCGGCTAACACCGGGGAGCTGTTTTTT 1303 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAAAGGGGCTTACCTACGAGCAGTACTTC 1304 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACCGTGGCTGATTCCTACGAGCAGTACTTC 1305 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGGGGGCTTCGACAGGGGAGACCACGAGCAGTACTTC 1306 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGAACAGGGAGGTTCTAGGGGCTACACCTTC 1307 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCTCCGGGACAGGGGGCGAGGAGACCCAGTACTTC 1308 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTACGACAGGGAGAGGTCACAGATACGCAGTATTTT 1309 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGAGGAAGGGGAGCTTTTCTTT 1310 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGCCCAGGGGACACAGCCTACGAGCAGTACTTC 1311 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCACAGTCCGAACACTGAAGCTTTCTTT 1312 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCACCATACAGGAGCCGAACACTGAAGCTTTCTTT 1313 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTACCAGCGTAGGAGGGTCCTACAATGAGCAGTTCTTC 1314 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCGAGGACAGGGGATATACGAACACTGAAGCTTTCTTT 1315 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGAAGAGTTACCAGGAGGGAACACTGAAGCTTTCTTT 1316 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGTCGCCGTGAGTGGGAGACCCAGTACTTC 1317 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTGATACTAGCGGGAGGAGGGCCGGGGAGCTGTTTTTT 1318 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCTAGCGACGCCGCCCTCCTACGAGCAGTACTTC 1319 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCTCACGGACAGGGTACTACAATGAGCAGTTCTTC 1320 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAATCGGGGTTGGGAACACTGAAGCTTTCTTT 1321 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGATTGGGACGGGACTAGCGCCGCCTACGAGCAGTACTTC 1322 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGATCCTACGCGGGGTGGGAGCTCCTACGAGCAGTACTTC 1323 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCAAAGGGGAGTTGGGGACACCCCCCCGGGAGACCCAGTAC TTC 1324 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTGGGGGGCGGGAGGATTGTACGAGCAGTACTTC 1325 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGGGTTGTAGCGGGAGGCAATGAGCAGTTCTTC 1326 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGTTGGTGAAGCTTTCTTT 1327 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTATCCCCGGGACTAGCGGACACAGATACGCAGTATTTT 1328 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCACCCCTTAGCGGGGGGTTGTACAATGAGCAGTTCTTC 1329 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTTGCAGGACAGGGCCGAATGGGAGATACGCAGTATTTT 1330 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCACCAACCGGGTCCTAGGGGACTATGGCTACACCTTC 1331 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGGGAGGGGCGCACCCACTGAAGCTTTCTTT 1332 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGTGGGGTAATCAGCCCCAGCATTTT 1333 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGATGGCGGGGGCAGGGAGACCCAGTACTTC 1334 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATAGGACACGACGGCATGAACACTGAAGCTTTCTTT 1335 18-TL615-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGAGGGACTAGCGGGGGGCCCTGCCCACAATGAG CAGTTCTTC 1336 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGATCAATGAGCAGTTCTTC 1337 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCTTTTCGGGGTTGGGCTACGAGCAGTACTTC 1338 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTAAGCGGGAGGGCACCGGGGAGCTGTTTTTT 1339 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTATTTTCGAGGGGAGACATTCAGTACTTC 1340 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCATTCGGACCGGGGGTGCTGGCAATCAGCCCCAGCATTTT 1341 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGACGGACAGGGGCGCCAGCACTGAAGCTTTCTTT 1342 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTGGGGGCTGTCCGGGGCCAGGAACGAGCAGTACTTC 1343 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGTCCAAGGGGGTTCCTACGAGCAGTACTTC 1344 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCATCCCCCACGGGGCTAGCGGGCTATACGAGCAGTACTTC 1345 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGTTGGGAGGACTGAACACTGAAGCTTTCTTT 1346 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGAGGTAGCGGGAGCGAGATACGAGCAGTACTTC 1347 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGCCACATTCCCACAGGGGGCCCCTTACAATGAGCAGTTCTTC 1348 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGTAACCGGGAGAAATAGCAATCAGCCCCAGCATTTT 1349 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGACGGGTCCTACAATGAGCAGTTCTTC 1350 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCCGATTTCTTAGCGGGAGTCTTGATGAGCAGTTCTTC 1351 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGAGTGGGGTTTGGGCCAAAACGGGGAGCTGTTTTTT 1352 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATAGGCTGGGGTAGCTCCTACAATGAGCAGTTCTTC 1353 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGGAGGGGATGGCTGAAGCTTTCTTT 1354 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGGAGGGGGTCCCCAAGAGACCCAGTACTTC 1355 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCGACAAAGACCAGCCCCAGCATTTT 1356 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAACGGGGACAGGGACCAAAAACATTCAGTACTTC 1357 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATTATCCGGGAAGCCCCAGCATTTT 1358 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCCTGCACCGGGACAGGGTAGTCGAGCAGTACTTC 1359 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGTACAATGAGCAGTTCTTC 1360 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGACCTAAAACTAGCGGGAGCCTCGATGAGCAGTTCTTC 1361 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGGAACGAGCAGTACTTC 1362 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTACGGGACAGGGACTTACGAGCAGTACTTC 1363 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTATACGGGGGCTATGGCTACACCTTC 1364 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTATCAGGGAGCTGGGGGGACACTGAAGCTTTCTTT 1365 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGACTCACTAGCGGATAGCACAGATACGCAGTATTTT 1366 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTATTCCGGGACTGGCCTACAATGAGCAGTTCTTC 1367 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGAAACTCGGGACGAGCAGTACTTC 1368 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGAACAGGGGGCTGAAGCTTTCTTT 1369 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGTAAGGCAGCTGGGGGAGCAGTACTTC 1370 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGAATCGGACAGAGCTCCTACGAGCAGTACTTC 1371 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGAGAGAGACCAATGGCTGAGACCCAGTACTTC 1372 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTACCAGCTCCTACGAGCAGTACTTC 1373 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTATTCGGGGTCTAGCCGCTACGAGCAGTACTTC 1374 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTTGGTAGTTGGAGCACCGGGGAGCTGTTTTTT 1375 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTTTTTCCTCAGGACAGGGGGCATACGAGCAGTACTTC 1376 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGGACTAGCGGACTACGAGCAGTACTTC 1377 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTCAGGGACACATCCTACGAGCAGTACTTC 1378 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTGAAACGGACGGTAACACTGAAGCTTTCTTT 1379 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGGTAACAATGAGCAGTTCTTC 1380 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCCACCGCTACGAGCAGTACTTC 1381 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCTTTTCGGGGTTGGGCTACGAGCAGTACTTC 1382 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGAGGACCACCTCACCGGGGAGCTGTTTTTT 1383 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCTAACAGGGGGCCGAGGGAGACTGAAGCTTTCTTT 1384 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGACAGGGTACGAAGCGGGGAGCTGTTTTTT 1385 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGACGGACTAGCGGGAGACACCGGGGAGCTGTTTTTT 1386 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGCGACGGAATCTCTGGGGCCAACGTCCTGACTTTC 1387 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCGCCCCGGAGAAACTAGCGGGAGTCTCCTACGAG CAGTACTTC 1388 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCTTTCCCGGGACAGAGTAATGAAAAACTGTTTTTT 1389 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCCAAAGGGGGCGTCTGATCCAGCCCCAGCATTTT 1390 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGGAGGGGGAAGCTATGGCTACACCTTC 1391 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGAACAGGGAGGTTCTAGGGGCTACACCTTC 1392 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGGTTCACAGGGGCTCACAGATACGCAGTATTTT 1393 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCACCGGGACTAGCGGAGCCAGTGAGCAGTTCTTC 1394 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGAGGAGAGTTACAATGAGCAGTTCTTC 1395 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCACTCAAAATGAGCAGTTCTTC 1396 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCAGGTTACAATGAGCAGTTCTTC 1397 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCTACAGGGGTCTCCTACGAGCAGTACTTC 1398 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACGGAGCGGGAGGGTTCCGAGCAGTACTTC 1399 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATTACCTAGCGGGGGGCCGGGCTGAGCAGTTCTTC 1400 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCATCGGACAGGGCCCTTCCTACGAGCAGTACTTC 1401 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACCAGGGGGGGAACTATGGCTACACCTTC 1402 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTCCTCCGGGACAGGGGTCGAGCAGTACTTC 1403 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTGAAAGAGTTGGCGACGAGCAGTACTTC 1404 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCACCAACCGGGTCCTAGGGGACTATGGCTACACCTTC 1405 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGATGCTTTCACAGATACGCAGTATTTT 1406 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCCCGACTAGCGGGAGGCGGTGAGCAGTTCTTC 1407 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCAGGGCTTACAATGAGCAGTTCTTC 1408 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGACAGAGAACACCGGGGAGCTGTTTTTT 1409 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTACTCCACCCTTTCTACGAGCAGTACTTC 1410 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCCCAGGGGACCACTACGAGCAGTACTTC 1411 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGATCGATCGGGCCTAGGGGACTTC 1412 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGAACTACGAGCAGTACTTC 1413 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTTACCCAGGGAAGGACTACACCTTC 1414 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGCCACATTCCCACAGGGGGCACCTTACAATGAGCAGTTCTTC 1415 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGCCTTGGGGGAAACACTGAAGCTTTCTTT 1416 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCGCCGGGACAGGAGACTACGAGCAGTACTTC 1417 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGACATCGTGAGCAATCAGCCCCAGCATTTT 1418 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGGGTTTGGGAGACCCAGTACTTC 1419 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGTCCCAGGGAACACTGAAGCTTTCTTT 1420 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGACTGACTGGGGGAACACCGGGGAGCTGTTTTTT 1421 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCAAAGACAGGGTTGAATGAGCAGTTCTTC 1422 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAATCTCTGAAGCTTTCTTT 1423 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAGGGACAGGGGACGCCTTTCGCTCCTAC AATGAGCAGTTCTTC 1424 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGGAGCCAACCGCTATGGCTACACCTTC 1425 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATCCGCAGGGGGAGGTGGCAATCAGCCCCAGCATTTT 1426 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGGTGTTGCCCAGTACTTC 1427 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGCGGGACTAGCGGAAATATTCTCCTACAATGAGCAGTTC TTC 1428 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCCCGGGGGGGTCAGCCCCAGCATTTT 1429 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAGACAGAGAACACCGGGGAGCTGTTTTTT 1430 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGTGCAGGGGTTCGCCGGGGAGCTGTTTTTT 1431 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGACACCCTCGGGGGGGGTGACACCGGGGAGCTGTTTTTT 1432 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCGTAGGGAACACTGAAGCTTTCTTT 1433 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCAGCTGGGAAGCAGTATTTT 1434 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGGGACAGGAGTCCTACGAGCAGTACTTC 1435 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCTGACAGGGGGCCGTAATCAGCCCCAGCATTTT 1436 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATCCCCGGCAGGGGGACACCGGGGAGCTGTTTTTT 1437 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTCGATGGGGCCAACGTCCTGACTTTC 1438 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAACGCGTGGAATGAGCAGTTCTTC 1439 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCAGGGGCCGGGGAGCTGTTTTTT 1440 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGCTATCTCTTTCGGGGAGCAGTACTTC 1441 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGCTCCCCGGGGGCGATGAGCAGTTCTTC 1442 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGAGACCCCCTCAACAGTACCTTTTTTT 1443 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTGCTAGCGCAGCAGTTCTTC 1444 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGGGTCAGGACCCCCAAATGAGCAGTTCTTC 1445 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGAAGAGAGGGGGGGAGGAGCAGATACGCAGTATTTT 1446 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTGGGGACAGATCAATTCACCCCTCCACTTT 1447 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTTACATCGGGAGCACAGATACGCAGTATTTT 1448 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAATAGGCTACGAGCAGTACTTC 1449 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCTCCCGACAGGGGCTGAAGATACGCAGTATTTT 1450 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGAATCTAGCGGGAGCCGGGGAGCTGTTTTTT 1451 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCATGGGGGGACAGAACTATGGCTACACCTTC 1452 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGAGGGGGAACAGTAGACAAAAACATTCAGTACTTC 1453 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATTGGCGTCTATGGCTACACCTTC 1454 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGAAAAGGGTCGGGTCCCGGACGGCCCTAGC GTCCCTTACAATGAGCAGTTCTTC 1455 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGGGGGCACTGAAGCTTTCTTT 1456 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTATGGCGGTAGATACAATGAGCAGTTCTTC 1457 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGGAAGGGCCTCGGGGACTGAAGCTTTCTTT 1458 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAAAGGGACGGCGGCGACTATGGCTACACCTTC 1459 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAATCGACCAGGGACAGCCGAAGAGCAGTTCTTC 1460 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCCATGGCGGAATCATTCGGCGGACCGAGGGG AATGAGCAGTTCTTC 1461 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGTCGGCGGGAGAGCTACGAGCAGTACTTC 1462 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGACCGCGGCAGATGAAGAGACCCAGTACTTC 1463 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTCTCGACAGCTCCTACGAGCAGTACTTC 1464 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATCCAGGGGGCCCTAACTCCTACA ATGAGCAGTTCTTC 1465 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGACCCCCTTTTGGTGGGGGTGGACA CTGAAGCTTTCTTT 1466 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGGGGGTTAATATGAACACTGAAGCTTTCTTT 1467 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCTGACAGGGAGTCGCAATCAGCCCCAGCATTTT 1468 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGTCCAGATACCTACGAGCAGTACTTC 1469 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGAGGGGGAGCCAAAAACATTCAGTACTTC 1470 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTACGGACTAGCCTACAATGAGCAGTTCTTC 1471 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCGCCGACAGGGGGCTGGTATGGCTACACCTTC 1472 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAAAGGGGTCACCGGGGAGCTGTTTTTT 1473 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCCTGCAGGGGTTAGCGGGAGAAGAGCAGTTCTTC 1474 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCACCGGTGGGTACTACGAGCAGTACTTC 1475 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTAGCAAGGGAACTGAAGCTTTCTTT 1476 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTACGATTTACAGGGGAGGTGGAGCTGTTTTTT 1477 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCCGGGCACTGAAGCTTTCTTT 1478 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCACTACAGGGTTATGCTGAAGCTTTCTTT 1479 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAAGACTCCCCGGGCACACTGAAGCTTTCTTT 1480 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTGGGACAGGGGGCGAGGAGACCCAGTACTTC 1481 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCAGCGGGGCCTAGGCTATGGCTACACCTTC 1482 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAGGATCCTACACCTACCTACGAGCAGTACTTC 1483 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTGGGACTATCTTACAATGAGCAGTTCTTC 1484 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCACCAACTCCGGGACCGCGAGGTCACCCCTCCACTTT 1485 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCGACAGCTACAATCAGCCCCAGCATTTT 1486 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTCTCCGGGACTATAGCAATCAGCCCCAGCATTTT 1487 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTGGGCCGTGGCGAGCAGTACTTC 1488 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCTAGCAGCTTAGGTCCTAGCGTGAGGGAGACCCAGTACTTC 1489 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTATGACTAGCCGGACGGATGAGCAGTACTTC 1490 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCACAACCGGGACCTTTCTACGAGCAGTACTTC 1491 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTAACCCTATAGCGGGAGGACCCTACAATGAGCAGTTCTTC 1492 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGAGTCGGGGAGCACCTACGAAGAGACCCAGTACTTC 1493 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGACTTTCCAGGGTCTAATCAGCCCCAGCATTTT 1494 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCCAGCGGGAGCAAATCAAGAGACCCAGTACTTC 1495 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAAAGGGTTTGAACACTGAAGCTTTCTTT 1496 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCACTAACTATGGCTACACCTTC 1497 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGGGGTACTAGCGGGGGCGCAGATACGCAGTATTTT 1498 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCAACAGGGGATCCTTACAATGAGCAGTTCTTC 1499 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGAAACAGGCTTCAATCAGCCCCAGCATTTT 1500 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGTGGGTAGCGGGATGGGATGAGCAGTTCTTC 1501 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGAGGACCCTCCTACGAGCAGTACTTC 1502 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCACTTCGGACAGGGGGCTTGCCGGGGAGCTGTTTTTT 1503 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCGCGGGACTAGCGGTTACAATGAGCAGTTCTTC 1504 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGATGGGACTAGCGGGAGTCGAGTCCAAAAAC ATTCAGTACTTC 1505 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCCCCCGGGGCCGAGCAGTACTTC 1506 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTTACCATCGGGGGAGACCCAGTACTTC 1507 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGCCCTGGGGGTAGTTCACCCCTCCACTTT 1508 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCGGTGCGGGGAAGGACTATGGCTACACCTTC 1509 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGCCTTTACAGGGGTGGAGCAATCAGCCCCAGCATTTT 1510 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTGGGGGCTGGCCGGGGCCAGGAACGAGCAGTACTTC 1511 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGATATAGTAGCGGGAGGGGGCGAGCAGTACTTC 1512 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCCTAGGGCGGGAGGGGAGCAATGAGCAGTTCTTC 1513 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTCTTACGGGAGGTCACAGATACGCAGTATTTT 1514 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCACCCCAGGGATCAACACCGGGGAGCTGTTTTTT 1515 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAGATCGGGAAGGCGGCGCTGAAGCTTTCTTT 1516 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAAGGCAGGGGCCGTCCTACGAGCAGTACTTC 1517 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCAACGAACCACGAACACTGAAGCTTTCTTT 1518 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATAGGACTGTCTAGTCTCAATGAGCAGTTCTTC 1519 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCTTACCTGGCATAGCCCCCATCAGCTCCTACGAGCAGTACTTC 1520 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGGGTTGTAGCGGGAGGCAATGAGCAGTTCTTC 1521 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGACAGAGGACAATGAGCAGTTCTTC 1522 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTACTCAGGTGGACACTGGAAACACCATATATTTT 1523 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGGGGGGAGCAAATACGAGCAGTACTTC 1524 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGTGGGGTAATCAGCCCCAGCATTTT 1525 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCCACCTACGCAGGGAGGTTTTGGGAGCCCCAGCATTTT 1526 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGTCAGGAGACCGTGAAGCTTTCTTT 1527 19-TL615-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTCGCGGGAGATCCGCCTACGAGCAGTACTTC 1528 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTTTCGGGAGGGCCCTACAATGAGCAGTTCTTC 1529 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCAAGTGGATTTTCAACTAATGAAAAACTGTTTTTT 1530 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGATAGGAGCGGGAGAGAGTATTTT 1531 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGATACCGGCAGCACCTCTTATGGCTACACCTTC 1532 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCAGCGGGATTGGGCCACGAGCAGTACTTC 1533 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGACTAGCGGTATTTACAATGAGCAGTTCTTC 1534 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCTCGGACAGGACACCGGGGAGCTGTTTTTT 1535 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCAGGGAAGGGAACTGAAGCTTTCTTT 1536 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTATGACTGGAGACTCAAAGAGACCCAGTACTTC 1537 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCTCCGGGACAGTCCTACGAGCAGTACTTC 1538 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGGTGGAGGGGGCACTGAAGCTTTCTTT 1539 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCTACAGGCCAAGAGACCCAGTACTTC 1540 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGCCTCGTTGACAGGGCCATTGTCCGAGCAGTACTTC 1541 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCCTCTTATTGGAGCGGTAAGCTCCTACGAGCAGTACTTC 1542 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAATTCGGGGGAGACACTGAAGCTTTCTTT 1543 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCACCAGCCCAGGGGGCCGGGGCTACACCTTC 1544 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCCGTCCGGGGGCAGGAAACACTGAAGCTTTCTTT 1545 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATGTTTTCTCCGGTCTCCTACAATGAGCAGTTCTTC 1546 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCATGGGGATCCCAATGAGCAGTTCTTC 1547 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGGGGGACAGGGGGAATGGGAGCTGTTTTTT 1548 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTCCTACTAGCGAACACAGATACGCAGTATTTT 1549 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTGGGGCAGGGGCGCAAATTCACCCCTCCACTTT 1550 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGGGACATTTCCCGAACCGGGCTACACCTTC 1551 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATGGCGGCGGGGGGGCGCATTGAGCAGTTCTTC 1552 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGGGACAGTCTGTGGACACCGGGGAGCTGTTTTTT 1553 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGACAGGGATCTCTCTGGAAACACCATATATTTT 1554 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGAAGCCGGGACAGGTGACGAGCAGTACTTC 1555 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGATGACCTTAAACCTGCCGAGCAGTACTTC 1556 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGGCCGAGAGGGCAGGGGAAGAGACCCAGTACTTC 1557 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTCGGCACGGAGGCTTTCTTT 1558 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGACAGCTATGGCTACACCTTC 1559 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGGACTTGGCAGGCCTTGAAGCTTTCTTT 1560 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGCGGGACAGGTAACTGTTCGCTACGAGCAGTACTTC 1561 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCCCTCTATGAGCAGTTCTTC 1562 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCAGGGTATAGCAATCAGCCCCAGCATTTT 1563 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAACTCGTCTCTGGAAACACCATATATTTT 1564 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGCGGGGTGCGAGACTACAAGAGACCCAGTACTTC 1565 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGCGAAGCCTTTCAATGAGCAGTTCTTC 1566 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTCTCGGGACTTCACAATGAGCAGTTCTTC 1567 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTAAGTCACAGGGACCCCTATGGCTACACCTTC 1568 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCTCCGGGACTAGGTACAGATACGCAGTATTTT 1569 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTTCGTGGGCTTGGAGCTTTCTTT 1570 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGACGGGGGCCTTCACACAGATACGCAGTATTTT 1571 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTCAATCCCTACAATGAGCAGTTCTTC 1572 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGATTCGGGGAGTGGCAATCAGCCCCAGCATTTT 1573 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCGGAGGCAGGGGGAACTACGAGCAGTACTTC 1574 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTGGGGACAGAACACCGGGGAGCTGTTTTTT 1575 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACCACAGGGCCGCCTTTGATAGCTTTCTTT 1576 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTATCAAATTCGGGCACTGAAGCTTTCTTT 1577 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCTTTGGGGGTGAACACTGAAGCTTTCTTT 1578 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCACCCGGGACAGGGGATTTACGAGCAGTACTTC 1579 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGTGAGTCTACAGGGGAGGACCAGCCCCAGCATTTT 1580 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGTCGTCGGGGAAAGAGCAGTACTTC 1581 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTTGATCTGGCGGCGGGGTCATCCACA GATACGCAGTATTTT 1582 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCACTTTGCCCTCAGGGGTTTACGAGCAGTACTTC 1583 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTACGGACAGGGGTAAAAGAGACCCAGTACTTC 1584 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGTGAACGCGGGGGGCCCCATGAGCAGTTCTTC 1585 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGCTCCGGGACAGGGGTTCCCCGAGCAGTACTTC 1586 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGACGACGGGGTTGATGAGCAGTACTTC 1587 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGTGGGGGGGCAGATACGCAGTATTTT 1588 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTATCGTGGGACAGGGATTGGATA CTCAACAATGAGCAGTTCTTC 1589 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCCAAGCCGTGGGGGACGTGGCAGATACGCAGTATTTT 1590 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTATACTTCCGGCTCCTACAATGAGCAGTTCTTC 1591 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCGCCGATAGCGGGAGAGTCGCTGAGCAGTTCTTC 1592 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGGTAGCACCCAATGAGCAGTTCTTC 1593 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTTCCCACACTGAAGCTTTCTTT 1594 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCTAGCAGCCTAGCGGGCGGGGACTACCACGAGCAGTACTTC 1595 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTAAGCTAGGGACAGGGAGGGACAATGAGCAGTTCTTC 1596 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCTCACCGGAGGACAGGGTGAGGAGCACTGAAGCTTTCTTT 1597 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTATCGGGACTTTCCCGGAGGAGATACGAGCAGTACTTC 1598 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGTTCTAGCTCCTAGCACAGATACGCAGTATTTT 1599 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTATACCCCCCGGGGAGCAATCAGCCCCAGCATTTT 1600 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTCCTAGAGAACCAAGAGACCCAGTACTTC 1601 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGCGGGGGCCTCGATGGCTACACCTTC 1602 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGGTTACAATGAGCAGTTCTTC 1603 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGATCCCAGGGGGTATGGCTACTATGGCTACACCTTC 1604 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTAGCGTTACTGACAGATACGCAGTATTTT 1605 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGAGTACGGGGGGGCCCAGAATGAGCAGTTCTTC 1606 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCTGGAGCGTAGCACGGTACACTGAAGCTTTCTTT 1607 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCACACTGGGGTCCACCGGGGAGCTGTTTTTT 1608 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTGACAGGGGGGACTGAAGCTTTCTTT 1609 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAAGTTCTAACCTTCTATGGCAATCAGCCCCAGCATTTT 1610 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGATGTTTGGACAGCCTATGGCTACACCTTC 1611 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGTTTTCCTCGGGGATCTACGAGCAGTACTTC 1612 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCAGGAATAGACAACTATGGCTACACCTTC 1613 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGTAGCGGGGCCATACAATGAGCAGTTCTTC 1614 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGTGGTGCAGTACAATGAGCAGTTCTTC 1615 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGTCTCTAGCGGGAACCCTCCAAGAGACCCAGTACTTC 1616 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGGGAGGGGATTAGGGTACGAGCAGTACTTC 1617 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGGTTACGGCTACGAGCAGTACTTC 1618 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTTCCCTTGGGACTAGCGGGGCCCCATCCTACGAGCAGTACTTC 1619 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCGCAGGACCTGAGCAGTTCTTC 1620 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTGGGCCCCGGGACAGCCCGACAATGAGCAGTTCTTC 1621 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGGAGACAGGGCTATGGCTACACCTTC 1622 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGCACGGCACTAGCGGTTACAATGAGCAGTTCTTC 1623 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGATTCTACAGACTCTGGGGCCAACGTCCTGACTTTC 1624 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGATCCCGGGGTTGTACGAGCAGTACTTC 1625 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGACGAGACAGGGGACACTGAAGCTTTCTTT 1626 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTAGAGGGCGGGAGGGCTTGGGAGACCCAGTACTTC 1627 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGTTCAGGTGAACACTGAAGCTTTCTTT 1628 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGCGCTGGACAGGGCAGGATGGCTACACCTTC 1629 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCTCCTGGGACGGCACTGAAGCTTTCTTT 1630 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCCCGAGGTACAATGAACACTGAAGCTTTCTTT 1631 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAATCTACCCAGGGGTATTCACCCCTCCACTTT 1632 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGGCTCCTCTCTCAGGAGATAGACGTACAGAT ACGCAGTATTTT 1633 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTAGCGGACACAAGAACACTGAAGCTTTCTTT 1634 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGAGGACGGCTCCTACGAGCAGTACTTC 1635 55-TL661-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGTCGGGACAGTGAACACTGAAGCTTTCTTT 1636 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGTTACAATGAGCAGTTCTTC 1637 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTCCTACTAGCGAACACAGATACGCAGTATTTT 1638 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAAGTGGATTTTCAACTAATGAAAAACTGTTTTTT 1639 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATAGGAGCGGGAGAGAGTATTTT 1640 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGCCGAGAGGGCAGGGGAAGAGACCCAGTACTTC 1641 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTGGGACAAGCCCTACGAGCAGTACTTC 1642 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCAAATCCGAACACCGGGGAGCTGTTTTTT 1643 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGACGAGACAGGGGACACTGAAGCTTTCTTT 1644 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCTCCGGGACTAACTATGGCTACACCTTC 1645 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAATTCGGGGGAGACACTGAAGCTTTCTTT 1646 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCCTCTATGAGCAGTTCTTC 1647 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGATACCGGCAGCACCTCTTATGGCTACACCTTC 1648 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAGGGAAGGGAACTGAAGCTTTCTTT 1649 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGCTCCTCTCTCAGGAGATAGACGT ACAGATACGCAGTATTTT 1650 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACCGGGGGGCCTACGAGCAGTACTTC 1651 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCATGGGGATCCCAATGAGCAGTTCTTC 1652 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTAGCCCCCGGGACAGGGGGCTACGAGCAGTACTTC 1653 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCCTAGCGTCTAGCACAGATACGCAGTATTTT 1654 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGACACCCCGCAGGCAGCAGTCTATGGCTACACCTTC 1655 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTACCGGGGCGGACGGGGCCAACGTCCTGACTTTC 1656 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAAGACAGGGACAGCTCCACCGGGGAGCTGTTTTTT 1657 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGACGACGGGGTTGATGAGCAGTACTTC 1658 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCTTCCGGGAGAGGTGAGCAGTTCTTC 1659 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCACCAGCCCAGGGGGCCGGGGCTACACCTTC 1660 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCCTCGACAGGGATTCAGCCCCAGCATTTT 1661 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGCGGGGGCCTCGATGGCTACACCTTC 1662 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGGTCAAGGGGGGGCTTGGGGCTACACCTTC 1663 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGATAGAGCGGGAGGGATTTGGGAA GAGACCCAGTACTTC 1664 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCACCTCTAGGGAGGCCTCCTACAATGAGCAGTTCTTC 1665 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATCGGAAGGGGACTCTCCTACGAGCAGTACTTC 1666 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTGGGGCAGGGGCGCAAATTCACCCCTCCACTTT 1667 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGTCTAGCGGGGACCGGGGAGCTGTTTTTT 1668 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAAGTGGATTTTCAACTAATGAAAAACTGTTTTTT 1669 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCCCTCCGGGACGGCTAGCTCTGGAAACACCATATATTTT 1670 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCTCTAGCGGTCCCTGGGGTGAGCAGTTCTTC 1671 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGTTCAGGTGAACACTGAAGCTTTCTTT 1672 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACGCTGGGGTCGGGGGGAGCTGAGCAGTTCTTC 1673 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCCCCACCTCGACAGCATTACTGAAGCTTTCTTT 1674 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTCACTCGGGGGGGGACTGAAGCTTTCTTT 1675 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGCCGGACGGAATAATATAGACACTGAAGCTTTCTTT 1676 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCACAGGGTGACGCGGATCAGCCCCAGCATTTT 1677 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTTGGGGAGGCTACACCTTC 1678 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCCGGGAGGACGGTAATAGCAATCAGCCCCAGCATTTT 1679 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCTCTGGTCGACAGGGCTCAAGAGACCCAGTACTTC 1680 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTTAGTTCTAAAGTGGCAGCCTACGAGCAGTACTTC 1681 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCAACAATGAGCAGTTCTTC 1682 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTATCTGGGGGTAACTGGGGCAGATACGCAGTATTTT 1683 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGGGGGACAGGGTCTGGCTACACCTTC 1684 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTACGACTAGCGGGGGGGCCACAGATACGCAGTATTTT 1685 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGAGGAAGGGACGCTCTACGAGCAGTACTTC 1686 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTACCGGGAGGGTCGACGAGCAGTACTTC 1687 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGACTTGGCAGGCCTTGAAGCTTTCTTT 1688 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCCCGGGGGGAGGAGAGACCCAGTACTTC 1689 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGGCGACGGAGGCACAGATACGCAGTATTTT 1690 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGATCCGGAGGGATTGGAGACCCAGTACTTC 1691 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGAAAATGGCTGGAAACACCATATATTTT 1692 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAGAAGACTAGCGGAAGAGACCCAGTACTTC 1693 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCTAGCGGGAGGGGTGAGCAGTTCTTC 1694 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATAGGTGGCAGCTACAATGAGCAGTTCTTC 1695 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTTTTAAAGGTGGGGCCTACGAGCAGTACTTC 1696 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGAACGCGGGAGGGCCGCGGTTCTTC 1697 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAATCGACTCCCAACCGGCATACGCAGTATTTT 1698 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGACAGGGGGCGAAGGCACTGAAGCTTTCTTT 1699 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTACAATGAGCAGTTCTTC 1700 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGCGGGGTCACTCGAAGTATCTAACTATGGCTACACCTTC 1701 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGGGACTAGCGAGTTTCCCCCTCTT CAAGAGACCCAGTACTTC 1702 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTGACAGGGGGGACTGAAGCTTTCTTT 1703 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCTTCGGGGGGGGGACCCAGTACTTC 1704 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAGAGGAAGGGGATGGCTACACCTTC 1705 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCCCGGGGCCTACGAGCAGTACTTC 1706 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGCCTCGACAGACATGAACACTGAAGCTTTCTTT 1707 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCAACTAGCGGGCTTCACAATGAGCAGTTCTTC 1708 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCCCCAATGGACCCAGCATTTT 1709 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCCTACAGGGGGCGTATGGCTACACCTTC 1710 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGCCTCCGGGGGGCGCGAGTACCCAGCCCCAGCATTTT 1711 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAATCCGCCGGGGCACAGCCCCAGCATTTT 1712 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGGCCGAGCGGGGGGGCGTTGGATGGCTACACCTTC 1713 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGATAGTCTAGCGGGACACGAGCAGTACTTC 1714 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCTAGTGGTTTGGACCCCTTGGGCACCGGGGAGCTGTTTTTT 1715 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGGACGTCTGAATGAGCAGTTCTTC 1716 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGATGCTTCCGGAGCTAACTATGGCTACACCTTC 1717 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTGAGGATCAGGGGTTGAGTGAGCAGTTCTTC 1718 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAGAAATCACGGGACAGGCTAATCAGCCCCAGCATTTT 1719 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCACCCGGGACAGGGGTACTTCTTC 1720 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCACCTTTGCGGCGAACACCGGGGAGCTGTTTTTT 1721 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAGGCTGAGGGGGGAGAAGAGCAGTACTTC 1722 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGCAGGAGGCTCCTACAATGAGCAGTTCTTC 1723 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCGGCCCAGCGGGACCTTTCTTT 1724 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGACATAAGGGGGACTGAAGCTTTCTTT 1725 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGCCCGAGTGTCCGGGCTCACTGAAGCTTTCTTT 1726 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTGGGCCCCGGGACAGCCCGACAATGAGCAGTTCTTC 1727 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCGGGGACGGTTTCTTTCTACGAGCAGTACTTC 1728 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTTCAGGGGGCCGGACAGATACGCAGTATTTT 1729 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGGGAGGCCCTATAATTCACCCCTCCACTTT 1730 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGATCCCGGGGTTGTACGAGCAGTACTTC 1731 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGACGGCAGGGTACAGAGACCCAGTACTTC 1732 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGACAGGGATCTCTCTGGAAACACCATATATTTT 1733 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGATATGACAGGGGGCGAGACCCAGTACTTC 1734 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCGACGGAGGGAAGAGACCCAGTACTTC 1735 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATCAAATTCGGGCACTGAAGCTTTCTTT 1736 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTGAGAATCCGGGAGTGGCAGATACGCAGTATTTT 1737 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAAAGAGGGCACTGAAGCTTTCTTT 1738 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGCTACAGGTTCCGACTATGGCTACACCTTC 1739 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGGGGGCAGGGGCTCTCAAGAGACCCAGTACTTC 1740 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGTACAGAGCACAGATACGCAGTATTTT 1741 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTGAGGGAGTTGGGGAGCTGTTTTTT 1742 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGACAACTACAATGAGCAGTTCTTC 1743 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCGCCCGCTTCAGGGGGCACTGAAGATACGCAGTATTTT 1744 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCACGGTGGGCTCCGGTGGAACCGGGGAGCTGTTTTTT 1745 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCGTGAGCTCCTACGAGCAGTACTTC 1746 56-TL661-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTGAGTCGGGACAGGGATACGAGCAGTACTTC 1747 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTACCCTCGATAGCAATCAGCCCCAGCATTTT 1748 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGATCCCAGGGGAGCTGGGGCCAACGTCCTGACTTTC 1749 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCTCCTAGGGCAGCGACGCAGTACTTC 1750 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGGACTTGGACCGCTACGAGCAGTACTTC 1751 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGACAGGGTCAGGAGAGCAGTACTTC 1752 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCTGGGGGGAGGGAACTGAAGCTTTCTTT 1753 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAAGGGGTGCCGGGGAGCTGTTTTTT 1754 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCTACCAGCCGGGACAGGGGCCCTCACA GATACGCAGTATTTT 1755 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCTCCTGGGGGGCCAAGATACGCAGTATTTT 1756 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGACAAGGGGATAGCAATCAGCCCCAGCATTTT 1757 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGTCCCAGGGAGCTCCTACAATGAGCAGTTCTTC 1758 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGATGTTACAGGGTCTGGGGCCAACGTCCTGACTTTC 1759 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGCAGGGAACACCGGGGAGCTGTTTTTT 1760 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGAGGAAGGGAGTGGGGCCAACGTCCTGACTTTC 1761 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGAGGGGGGTACTGGGGCCAACGTCCTGACTTTC 1762 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTATCGGAGGGAGGGACAGATACGCAGTATTTT 1763 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAGCCTGTTACGGGACTAGCGGGGCGGAGCTC CTACAATGAGCAGTTCTTC 1764 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCGGACTAGCGGGGGCCCCAATGAGCAGTTCTTC 1765 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTTGGAGGGGACGGCTACGAGCAGTACTTC 1766 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGAGGCCCCTGGGCCCCAGCATTTT 1767 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTAAGGAGGGACAGGGACGGAAACACCATATATTTT 1768 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGACTAGAGGACAGCATAAGCTCCGAGCAGTACTTC 1769 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGGGGGGAACACTGAAGCTTTCTTT 1770 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTATCCCCGGGGGCCAGCAATCAGCCCCAGCATTTT 1771 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCCCCGGGACTAGCGTCGGAGACCCAGTACTTC 1772 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGATGGGACCCGCGACAATGGCTACACCTTC 1773 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACCCGTTGGGCCCGACAACAGTTCTTC 1774 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGAGGGACTAGCGGGGGGCCCTGCCCAC AATGAGCAGTTCTTC 1775 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCCAAGTACTAGCAATGAGCAGTTCTTC 1776 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTAGTGGCGGGGTAGCCTACAATGAGCAGTTCTTC 1777 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCTGGGGGGGGGAGCCAAAAACATTCAGTACTTC 1778 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCGCCTTGGCAAGCGGGAGAGGGGGAGCAGTACTTC 1779 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTGGACTAGCACCGGGGAGCTGTTTTTT 1780 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCGCCGGCTAGCGGGGGGGGCGCG GATGAGCAGTTCTTC 1781 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCAAAGGGGAGTTGGGGACACCCCCCCGG GAGACCCAGTACTTC 1782 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAGGGGCTGTCCTACGAGCAGTACTTC 1783 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCGTCGGGACAGGACTACGAGCAGTACTTC 1784 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCCGGGCCAAACTACGAGCAGTACTTC 1785 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTCGTCGCGGGCACAGATACGCAGTATTTT 1786 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTATATGGCGGCTACGAGCAGTACTTC 1787 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCGACAGGGTGGGAGACCCAGTACTTC 1788 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCCTGCCCTACGGGATGGGCACAGATACGCAGTATTTT 1789 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGAGACAGGCTCTGGGGCCAACGTCCTGACTTTC 1790 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTCGGGACTGACTACGAGCAGTACTTC 1791 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTTGGCACAAGGACTAGCGGGAGGTAC TCGATCCAGTTCTTC 1792 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCACTAGCGGGAGGGCCGTATGTCCCGAGTGA GTACGAGCAGTACTTC 1793 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCTGTTGGGGTTACTAACTATGGCTACACCTTC 1794 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTGGAGGGTCGGCAAGAGACCCAGTACTTC 1795 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGCGGGAGCCTACGAGCAGTACTTC 1796 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGTTGGAGGGGGGGTTAATGAGCAGTTCTTC 1797 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCAGGGGCGGGACGGCCCGATACAATGAGCAGTTCTTC 1798 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGAGAAGGAGGCAGGGGAGACCCAGTACTTC 1799 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGAAGGTGTGTCAGTGAACACTGAAGCTTTCTTT 1800 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCATGGGACAGGAGATCCTAGTCGCTACGAGCAGTACTTC 1801 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCTGGAGCCCCGGGGGACCGTACCGAAACGAGCAGTACTTC 1802 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTACTGCACAGGGATCGAACACTGAAGCTTTCTTT 1803 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCACCCCAGGGATCAACACCGGGGAGCTGTTTTTT 1804 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGGACAGGGTCGGGGAGCTGTTTTTT 1805 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAGGTTGGGAGATACGAGCAGTACTTC 1806 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGGGTGGCACTGAAGCTTTCTTT 1807 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGATCCTACGCGGGGTGGGAGCTCCTACGAGCAGTACTTC 1808 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCGAGAGACCGAACACCGGGGAGCTGTTTTTT 1809 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTTTGGGGACACTGAAGCTTTCTTT 1810 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCGGGGACAGCCTATGGCTACACCTTC 1811 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTATGGCAGGGAACGAACACCGGGGAGCTGTTTTTT 1812 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGGGAGGGACAGGGGGTCAGATACGCAGTATTTT 1813 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTACTACGAGGAAAACTGTTTTTT 1814 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCGGGACCGAACTACGAGCAGTACTTC 1815 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAGACTGGGACAGGGAACACTGAAGCTTTCTTT 1816 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACCAGGGAGGAGACTATGGCTACACCTTC 1817 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTATGGTCGCTAGCGGCCAAAGAGCCCCAGTACTTC 1818 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGATTTCACCACCGGGGGAGCTACGAGCAGTACTTC 1819 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGACTAGCGGGAGCCTTAAGGTTCGAGCAGTTCTTC 1820 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCAGCACAGACTGGGGGACTGAAGCTTTCTTT 1821 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGACCGGGACAGGGTTTTAATGAGCAGTTCTTC 1822 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTCCTTACGAGACGTGGGCGAAGATCGAGAACACT GAAGCTTTCTTT 1823 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTACCGCGACAGGGGATGGCTACACCTTC 1824 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCTGGAGGAGCCGGACAGGGTGGCACGAGCAGTACTTC 1825 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTACCCTCGATAGCAATCAGCCACAGCATTTT 1826 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGCCCGGGAAGGGGCCTACGAGCAGTACTTC 1827 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCTGGAGTACCCGGGACACCTACGAGCAGTACTTC 1828 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCGTTCGGGACAGTTGATCAGCCCCAGCATTTT 1829 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTAGGGACAGATTACGAGCAGTACTTC 1830 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGATCTCCTTCCTCCGGGACAGTAATATCTTAC AATGAGCAGTTCTTC 1831 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCACGTCGGGACTAGCGGTTACGAGCAGTACTTC 1832 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGATCCGCACGGGGCCAGGAACGAGCAGTACTTC 1833 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGTGACAGAGAACACTGAAGCTTTCTTT 1834 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGAGTCGTACGGACCAAAACAAGAGACCCAGTACTTC 1835 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTACCCCCACACAATGAGCAGTTCTTC 1836 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCACCAGCCCAGGGGGCCGGGGCTACACCTTC 1837 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAAGAGGGAACACTGAAGCTTTCTTT 1838 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCCCCGGGACTAGCGGGGTCCTACGAGCAGTACTTC 1839 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGCGGGGACGGTTGGAACTGAAGCTTTCTTT 1840 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCACCCTGTCCCCGGGACAGGGGGCCTCCGGGGAGCTGTTTTTT 1841 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGCTGACAATGAGCAGTTCTTC 1842 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCCGGGGTAGCGGGAGAATTTTACGAGCAGTACTTC 1843 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTTCGGCCCCCTCCCTACGAGCAGTACTTC 1844 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGGAGCGGGAGCCCCCGTTGAGCAGTTCTTC 1845 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTGGGGGGCTCAGCCCCAGCATTTT 1846 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCACAGACGGACAGGGTATAGACATTCAGTACTTC 1847 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAGGGACAGGCCTTGTACACCGGGGAGCTGTTTTTT 1848 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTCACAAGAGATACGCAGTATTTT 1849 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCCGATATGGGAATGAGGGAGAGCACA GATACGCAGTATTTT 1850 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGGCCGGTGGGGAACACTGAAGCTTTCTTT 1851 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTACGACCCCGGGACAGGGTACAAACTATGGCTACACCTTC 1852 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCGCTTACTAGCGGTACGAACACCGGGGAGCTGTTTTTT 1853 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGGTCGACAGGCGAAAAACTGTTTTTT 1854 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCGTAGCGGGAGGGTTGTTGTATGAGCAGTTCTTC 1855 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACGGCAGCAAGCAAGAGACCCAGTACTTC 1856 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGGAGTGGAGAATGAGCAGTTCTTC 1857 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCTTACGGATGGTCAAGAGACCCAGTACTTC 1858 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGTGATGTAGCGGGAGGTTACGAGCAGTACTTC 1859 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTCGTCAGTCCCGGCTACGAGCAGTACTTC 1860 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGTTCCGGGGTACCGGGGAGCTGTTTTTT 1861 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCAGCGGCGATGAACACTGAAGCTTTCTTT 1862 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAAGAGCAGGGCCGGCAGTCCCTACGAGCAGTACTTC 1863 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACGGGGCCTCATACGAGCAGTACTTC 1864 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGCCCCGACAGAACTTAACTATGGCTACACCTTC 1865 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATAGTGCAATTCTACGAGCAGTACTTC 1866 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGATCTAGGGATGCACAATCAGCCCCAGCATTTT 1867 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCACTAGCGGGGACCTTGTACCAAGAGACCCAGTACTTC 1868 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCATGGGACAGGGGATTGCAAGATACGCAGTATTTT 1869 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGAGTCGATAGGGTACGAGCAGTACTTC 1870 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCAGAGATCGGGTTGGACAGGCGAACGGGGAGCTGTTTTTT 1871 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTCAGAACTAGCAACGCGCAGTATTTT 1872 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTGGATAGTAAGGGCCCTCCTCGCGACGAGCAGTACTTC 1873 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATGGATCGTGCTAGCACAGATACGCAGTATTTT 1874 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGCCCAGGGGGCGGACACTGAAGCTTTCTTT 1875 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGAGCCTACAGGGAGCTGGGCACTGAAGCTTTCTTT 1876 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACCCGGCAACTAATGAAAAACTGTTTTTT 1877 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGGTCGGGGGCCGGGAGACCCAGTACTTC 1878 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCTCCGGGACAGGGGGCGAGGAGACCCAGTACTTC 1879 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACGGCAGCAAGCAAGAGACCCAGTACTTC 1880 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGCACAGGGGGCTGGTAATTCACCCCTCCACTTT 1881 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCGGGCCTACTAGTGACTCCTACAATGAGCAGTTCTTC 1882 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGACCGGACAGCGACAGATACGCAGTATTTT 1883 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCTACATCAGGGACCTTCCTACGAGCAGTACTTC 1884 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCGAGGTCAATTAACACCGGGGAGCTGTTTTTT 1885 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCACCATACAGGAGCCGAACACTGAAGCTTTCTTT 1886 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGAAGCAGAAGGTGGCTACACCTTC 1887 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAAGTTGTCGGAGGGCTCGAGCAGTACTTC 1888 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGAAGAGTTACCAGGAGGGAACACTGAAGCTTTCTTT 1889 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCTATTACAGGGGAAAAGAGACCCAGTACTTC 1890 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAAAATGGAGGGAGGGCCCTCCTACGAGCAGTACTTC 1891 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTTGGGGGGGATCAGCCCCAGCATTTT 1892 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGCGACCGCTGCAGGTAATCAGCCCCAGCATTTT 1893 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGGGGACAGGGGTGGAAGCTTTCTTT 1894 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGGGACAGGGATATTCCTACGAGCAGTACTTC 1895 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCTCGGGACTCCTACCTACGAGCAGTACTTC 1896 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAAGTATTAGCCATGAGCAGTTCTTC 1897 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATCGGTGCGGGAGCCCCGTTTGACATTCAGTACTTC 1898 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTATTCTTATAGCACAGATACGCAGTATTTT 1899 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACAGCGGGACAGGGGGCTCGTGGAAACACCATATATTTT 1900 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCACCCCTTAGCGGGGGGTTGTACAATGAGCAGTTCTTC 1901 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTCCATTCCGGCTTTTACGAGCAGTACTTC 1902 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGGGAGGGGCGCACCCACTGAAGCUTCUT 1903 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTAGCCGCCGACTGGAAGTCCTACGAGCAGTACTTC 1904 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGACTAGCGGCTGGCAATGAGCAGTTCTTC 1905 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGGGGACAGATACGCAGTATTTT 1906 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATAGGACACGACGGCATGAACACTGAAGCUTCUT 1907 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCGGCTGGACAGGGCCTGAGACCCAGTACTTC 1908 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCTGGGACAGCTCTCGAGCAGTACTTC 1909 63-TL663-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGATCCCAGGGGAGCTGGGGCCAACGTCTTGACTTTC 1910 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCACAGGGCAATAAGATCGAGCAGTACTTC 1911 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGCACAGGGGGCTGGTAATTCACCCCTCCACTTT 1912 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGTCGTGGGCTCGAGCTACGAGCAGTACTTC 1913 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCGCAGCAAGCCAAAAACATTCAGTACTTC 1914 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATCGTATGGGGGAAATTCACCCCTCCACTTT 1915 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCATGGGACAGGAGATCCTAGTCGCTACGAGCAGTACTTC 1916 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTTGGTAGTTGGAGCACCGGGGAGCTGTTTTTT 1917 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCGACCGCTGCAGGTAATCAGCCCCAGCATTTT 1918 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTTCAGGGGACTCCTACAATGAGCAGTTCTTC 1919 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCGGGCCAAACTACGAGCAGTACTTC 1920 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAAGCTGGGAGAACACTGAAGCTTTCTTT 1921 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTACCACTTTCAGGTGGACACCGGGGAGCTGTTTTTT 1922 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCTACCAGCCGGGACAGGGGCCCTCACAGATACGCAG TATTTT 1923 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGGGGGAACACTGAAGCTTTCTTT 1924 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCATGTGGGGGCCCCGGAGGGGCACTGAAGCTTTCTTT 1925 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCCGGGACAGCTTACAATCAGCCCCAGCATTTT 1926 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCATCGGACAGGGCCCTTCCTACGAGCAGTACTTC 1927 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGTGGGGGCATGGGGGAGCAGTACTTC 1928 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAAGACAGGGGGTAGCACAGATACGCAGTATTTT 1929 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTATCCCCGGGGGCCAGCAATCAGCCCCAGCATTTT 1930 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAGTCCCATCTCCTACGAGCAGTACTTC 1931 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCCCCGGACACCTACGGCGGGGAGCTGTTTTTT 1932 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTGGACTAGCACCGGGGAGCTGTTTTTT 1933 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTAAACAGGGGGCGACCACTGAAGCTTTCTTT 1934 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCACCGCAGGGGGAGGCGTAACCCAGTACTTC 1935 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAAGGCAGGGGCCGTCCTACGAGCAGTACTTC 1936 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCAGACAGGGGGCTTTGAATGAGCAGTTCTTC 1937 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTACGGGGGAACACTGAAGCTTTCTTT 1938 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTATCGGAGGGAGGGACAGATACGCAGTATTTT 1939 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCCACCGCTACGAGCAGTACTTC 1940 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAAGGGGAAATTCACCCCTCCACTTT 1941 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGACAGGGTACGAAGCGGGGAGCTGTTTTTT 1942 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGATCCGCACGGGGCCAGGAACGAGCAGTACTTC 1943 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCAGGAACCCCCGGGGCTTTCGAGCAGTACTTC 1944 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGGAGCGGGAGCCCCCGTTGAGCAGTTCTTC 1945 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCTCCGGAACGGATATAAACTGTTTTTT 1946 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATTACCTAGCGGGGGGCCGGGCTGAGCAGTTCTTC 1947 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTGGCCGACAGGGCCGTAGCAATCAGCCCCAGCATTTT 1948 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAGACGGGCCGGAGCAGTTCTTC 1949 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAATGTGGGACCAAATAATTCACCCCTCCACTTT 1950 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGTTGGGACAGGGGGACTATGGCTACACCTTC 1951 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAGTTGTCGGAGGGCTCGAGCAGTACTTC 1952 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCACCGGGACTAGCGGAGCCAGTGAGCAGTTCTTC 1953 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTTCAGATACGCAGTATTTT 1954 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGGTGGCACTGAAGCTTTCTTT 1955 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATAGGAGGTACGGACGAGCAGTACTTC 1956 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCAACGAACCACGAACACTGAAGCUTCUT 1957 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGTCCCAGGGAGCTCCTACAATGAGCAGTTCTTC 1958 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCGTCGGGACAGGACTACGAGCAGTACTTC 1959 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATCGGTCTAGCGGGAGGAUGGTGCAGATACGCAGTATTTT 1960 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTCCCTTGTTCCGGACTAGCGGGGGGGCCGATTGGGAGCAGTTC TTC 1961 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGTCCAGATACCTACGAGCAGTACTTC 1962 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTATACCGTGGCCCACACCGGGGAGCTGTTTTTT 1963 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCCACAGGGGACCTGAACACTGAAGCTTTCTTT 1964 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAAGCATAGGCACAGGCACCTTTGACGAGCAGTACTTC 1965 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTCACAGGGGCCTACGAGCAGTACTTC 1966 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAGGGACTCTTGGGCAGTTCTTC 1967 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGTTAAAGGGGACAGGGATGAACACTGAAGCTTTCTTT 1968 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCGGACTGGGGATTTACGAGCAGTACTTC 1969 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGTCTTGGCAGTACGAGCAGTACTTC 1970 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACAACCCCCGGGACAGCTTCTGAAAAACTGTTTTTT 1971 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCCGCGACTCCTTGGGCGAGCAGTACTTC 1972 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTATGGTCGCTAGCGGCCAAAGAGCCCCAGTACTTC 1973 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTGGACAGGGGTTCTACGAGCAGTACTTC 1974 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTATATGGCGGCTACGAGCAGTACTTC 1975 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCGACAGAAGGGAAAAACTGTTTTTT 1976 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGGACGCAGGGTCGGCACAGATACGCAGTATTTT 1977 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCGACATTTCTAACTATGGCTACACCTTC 1978 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCGCCGGGACAGGAGACTACGAGCAGTACTTC 1979 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCCTCGTAGGTGGCAATCAGCCCCAGCATTTT 1980 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGGTGCAACCGGGGAGCTGTTTTTT 1981 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTACCCCCGACAGGGCCGGATTACGAGCAGTACTTC 1982 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGAGGAAAACATTCAGTACTTC 1983 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTGCTGTTAGGGAGCAATCAGCCCCAGCATTTT 1984 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCTTGACAGGGGGCGCGAACACTGAAGCTTTCTTT 1985 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGTCGACAGGGGAGTACTTC 1986 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGGGCCCGGCGGGGGAGCAGTACTTC 1987 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCAGTGCGGGAGGGCCATACGATGAGCAGTTCTTC 1988 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCCCTAGCGGCCAGCTCCTACAATGAGCAGTTCTTC 1989 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGTCCCAGGGAACACTGAAGCTTTCTTT 1990 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTGGCACAAGGACTAGCGGGAGGTACTCG ATCCAGTTCTTC 1991 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGAGTTGGGGAAGCGGGGGTGAGCAGTACTTC 1992 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGAGGCTCGGTGAGCAGTTCTTC 1993 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAGGGGTAGGGGGAGCAACTAATGAAAAACTGTTTTTT 1994 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGGGGGACAGAACTATGGCTACACCTTC 1995 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAACAAGCCCAGGGGCCACTGAAGCTTTCTTT 1996 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTACGAACACTGAAGCTTTCTTT 1997 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCACAGGGCAATAAGATCGAGCAGTACTTC 1998 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGTACTCTGAGGACGGGAACTACGAGCAGTACTTC 1999 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGAGTACTTGGGACAGGGAGGCCACCGGGGAGCTGTTTTTT 2000 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACAGCGGGCTCGAACACCGGGGAGCTGTTTTTT 2001 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCCACCGAGATTCAGCCCCAGCATTTT 2002 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCGGGGCGGGGGGGACAGAGACCCAGTACTTC 2003 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGGGGGATCAGCGGACCGCTCCTACAATGAGCAGTTCTTC 2004 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGACCCCACTAGCGGGAGCTACGAGCAGTACTTC 2005 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATCTCGCACAGATACGCAGTATTTT 2006 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGCTAGCCGGTAACGAGCAGTACTTC 2007 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGCTGGCACAGATACGCAGTATTTT 2008 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGGGACAGGGGCAGGCCTAGAGGACTACACCTTC 2009 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCTAACAGGGGTGGGTATTCACCCCTCCACTTT 2010 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATTGGGGTGGAAGACGAACACCGGGGAGCTGTTTTTT 2011 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGTTGGGACAGGGGGACTATGGCTACACCTTC 2012 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCACCTGGGGCCAACGTCCTGACTTTC 2013 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGTACAGGGGGGTGGCTATGGCTACACCTTC 2014 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGTGCAGGGGTTCGCCGGGGAGCTGTTTTTT 2015 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCAGGGGCGGGACGGCCCGATACAATGAGCAGTTCTTC 2016 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGTGGACAGTTACAATGAGCAGTTCTTC 2017 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGCAACAGGGGGATATAGTCAGCCCCAGCATTTT 2018 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGACAGCTCTGGAAACACCATATATTTT 2019 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATTTCTCTTCGAGCAGTACTTC 2020 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCACCAAAGTTCTGGTCAGCCCCAGCATTTT 2021 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGTACGTTCCCTAACCTCCTACGAGCAGTACTTC 2022 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGAGTGTACAGCTGCTTCCTAAGGGTGTTGAGCAGTTCTTC 2023 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCGGGGGGGACAGGGCGGACTCTGGGGCCAACGTCCTGACTTTC 2024 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATATTGAAAGGCTACGAGCAGTACTTC 2025 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTACAGACAGTAGTGAGCAGTTCTTC 2026 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGAGCTTACGGGCACAGATACGCAGTATTTT 2027 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGTCACGAATCCTACGAGCAGTACTTC 2028 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCACTTCGGACAGGGGGCTTGCCGGGGAGCTGTTTTTT 2029 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGACTTATAGAGGGTTCCGAGCAGTACTTC 2030 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGCAAGACGGTCGAACTGAAGCTTTCTTT 2031 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCACGGATTCTCTGGAAACACCATATATTTT 2032 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTCAGCAAGAACACTGAAGCTTTCTTT 2033 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGATTTGGACGCTAGCACAAACCACAATGAGCAGTTCTTC 2034 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCGCCGGCTAGCGGGGGGGGCGCGGATGAGCAGTTC TTC 2035 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCMCGGCGCCTCTGGGGCCAACGTCCTGACTTTC 2036 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGACAGCCGGGTATGGCTACACCTTC 2037 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGATAGCTACAGATACGCAGTATTTT 2038 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGACAGGGCTACGAGCAGTACTTC 2039 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATCGGTGCTCTCCTACAATGAGCAGTTCTTC 2040 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTACATCAGGGACCTTCCTACGAGCAGTACTTC 2041 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACGACGGACAGAACACTGAAGCTTTCTTT 2042 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGAGGTCAATTAACACCGGGGAGCTGTTTTTT 2043 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGATCCGGGCCAAGAGACCCAGTACTTC 2044 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATTAGACAGGGGGATGAGCAGTTCTTC 2045 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGACATCCGGGACAGGGGCCACGAGCAGTACTTC 2046 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCCTAGGGCGGGAGGGGAGCAATGAGCAGTTCTTC 2047 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTCCCCTTTTCGAGCGGGAAGCTCCTACGAGCAGTACTTC 2048 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTTCCAGCAGCTCCGGGCCAAACTACGAGCAGTACTTC 2049 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGGGACTAGCTTTACTCACAGATACGCAGTATTTT 2050 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGGGGCCCCGGGGAGCTGTTTTTT 2051 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCTAAGTGGACCTATGGCTACACCTTC 2052 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAATTTTTCTGGCAGGGGGCTTTTTGTTCGAGCACT GAAGCTTTCTTT 2053 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAGTTGCTGGGGGAGACACAGATACGCAGTATTTT 2054 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGGGGGCACTGAAGCTTTCTTT 2055 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGACAGGGGCGAAAAACACTGAAGCTTTCTTT 2056 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCCACGCCGATGTTAGCGGCCCAAGGGAGCTCCTACAAT GAGCAGTTCTTC 2057 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCCTTAACAGGGGTCTCTATAATTCACCCCTCCACTTT 2058 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAATCGACCAGGGACAGCCGAAGAGCAGTTCTTC 2059 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAATCCACACAGATACGCAGTATTTT 2060 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCGGGGGCGGCCGGGGATTCACCCCTCCACTTT 2061 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAAGTCCAGGGGGCATTCAGTACTTC 2062 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCGGGACCGATGAGCAGTTCTTC 2063 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGACCACGGGACTAGCCCTCACAATGAGCAGTTCTTC 2064 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGGAGGCCGGGACTAGGTACGAGCAGTACTTC 2065 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCCCGCGGCGCCAACGTCCTGACTTTC 2066 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGAGAGACCGAACACCGGGGAGCTGTTTTTT 2067 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCTTCCAACAGCCGGCGCCAACGTCCTGACTTTC 2068 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCACACTAGCGGGGCGAACACCGGGGAGCTGTTTTTT 2069 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGCCCTGGGACAGGCGGGGAACACTGAAGCTTTCTTT 2070 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCACCCCCCAGGCGCCATCCTACGAGCAGTACTTC 2071 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGGCTAGCGGGAGACAATGAGCAGTTCTTC 2072 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGTACCCTACCCTCCTACGAGCAGTACTTC 2073 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGAGGGACAGGCGAGCTCCTACGAGCAGTACTTC 2074 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACCTAAAGACAGGGGAGGGCTATGGCTACACCTTC 2075 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGATCGGACCAAGAGACCCAGTACTTC 2076 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGTTTAGCGGGGATGAGCAGTTCTTC 2077 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGGGACTGAACACAGATACGCAGTATTTT 2078 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCTCGGACAACCCAAACTACGAGCAGTACTTC 2079 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTCACCGAACTAAGGACAGGGACCTTAAGGATGAG CAGTTCTTC 2080 64-TL663-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGGCTGGACAGGGCCTGAGACCCAGTACTTC 2081 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTGGGGATTCCGGCGGGACTATGGCTACACCTTC 2082 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGAGTGCGGGAGCTTCACAGCGTGCCCAGATACG CAGTATTTT 2083 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGACCCGGGGGAAGTTCGACTACTAGCACAGATACG CAGTATTTT 2084 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAGCAGGGGACCTTATGGACAGATACGCAGTATTTT 2085 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACCCTAGGACTGCAAGAGACCCAGTACTTC 2086 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAGGGGGGGGACGGCCCCTACAATGAGCAGTTCTTC 2087 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGCTGGGCCAGGGACGAACACTGAAGCTTTCTTT 2088 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTACTAAGGGCCTACGAGCAGTACTTC 2089 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCGGCAGGAGCCTCCTACGAGCAGTACTTC 2090 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTTGGTATGAACACTGAAGCTTTCTTT 2091 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTAGAGACAGGGCCGTACTTT 2092 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCCCAATATTTTTACACTGAAGCTTTCTTT 2093 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCTTCGGGTGAGCAGTTCTTC 2094 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCCCGACTAGCGGGAGCTATAGATACGCAGTATTTT 2095 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTGGGGAACGGGGTACGAGCAGTACTTC 2096 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGAGTCGACAGGGTTAAATACGCAGTATTTT 2097 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCGTACTGGGGACTAGCAACGATGAGCAGTTCTTC 2098 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCGCGAGTAGGGAGTAATTCACCCCTCCACTTT 2099 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTACGGGACAGGGGGCGGATGGCTACACCTTC 2100 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCATTGTCTAGTAGCCACAATGAGCAGTTCTTC 2101 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGCACAGACAGGGTCTTACTATGGCTACACCTTC 2102 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTTATACAGGGACTCGATGGCTACACCTTC 2103 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGGACAAGCCTACGAGCAGTACTTC 2104 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTCCGGGACATAAGACAGTATTTT 2105 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGATCCAGGGTATTACAATGAGCAGTTCTTC 2106 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTCAGGACTAGCTCCTACAATGAGCAGTTCTTC 2107 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGTCCTCATATCCAGAGCTCCTACGAGCAGTACTTC 2108 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGATATGGAGGGCAAGGTCGATGAGCAGTTCTTC 2109 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTAGGGGGGACTCCGTTCAATGAGCAGTTCTTC 2110 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCCAAAGTACTAGCGGGATATCCACCGGGGAGCTGTTTTTT 2111 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAACCAGATACGCAGTATTTT 2112 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTCGGAGGGAACACTGAAGCTTTCTTT 2113 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGGCCGAGGAAATCTATAGCAATCAGCCCCAGCATTTT 2114 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCGGGCCGGGACTAGCGGGAGGGCTTTACGAGCAGTACTTC 2115 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGGAGTGACGGAGACGGAGACCCAGTACTTC 2116 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTATCAGGACAGGGCCAAATAGCAATCAGCCCCAGCATTTT 2117 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAAACGAAGATCAGTAGCACAGATACGCAGTATTTT 2118 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCTAACCGTGGACAGGGGGCCTCTCTTC 2119 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGGGGAACAGGGCTCGACTCCTACGAGCAGTACTTC 2120 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACCTCACTCCCGGAGAGGTTGGAGACCCAGTACTTC 2121 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTCAGCGGGATGGGTTCCTACGAGCAGTACTTC 2122 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGGGTTAAGAGGGATGAGCAATCAGCCCCAGCATTTT 2123 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGCGTTGAAGAGAGGGCGTGGGGAATGAGTGAGACCCAGTACTTC 2124 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGTTACAGAAAACACCGGGGAGCTGTTTTTT 2125 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACGGGACAAGTCTCAATGAGCAGTTCTTC 2126 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTGGATCAACCCGGGACTAGCCTCGAACTAC GAGCAGTACTTC 2127 90-TL101-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCATTGTGGGGAGGTCGCCTGCCGGTGAGCAGTTCTTC 2128 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGACAAGCCTACGAGCAGTACTTC 2129 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTGGGGAACGGGGTACGAGCAGTACTTC 2130 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGACCTTCTAGACATTGAGGCCGGGGAGCTGTTTTTT 2131 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTGCAAGGACTAGCGGAAGGCTCCTACAATGAGCAGTTCTTC 2132 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGTCGGGACTAGCGGGAGGCTGGGAGCAGTTCTTC 2133 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTAACCGTGGACAGGGGGCCTCTCTTC 2134 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACACTAGCGGGGACAATGAGCAGTTCTTC 2135 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCTTTGCCCGGACTAGCGGCGGCGGTGAGCAGTTCTTC 2136 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGCCCGACCTACCTCGCAGGGGCCCCAGCATTTT 2137 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAAGCCAGGGGACCCAGCCCCAGCATTTT 2138 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGGGTTAGCGGTTAGCTCCTACGAGCAGTACTTC 2139 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCCGGACCGAGCACTGAGCAGTTCTTC 2140 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTACTAGCGGGAGTCGACACCGGGGAGCTGTTTTTT 2141 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCCTAGGACTGCAAGAGACCCAGTACTTC 2142 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGTTGGGACGAGCGGCAGCCCCTACGAGCAGTACTTC 2143 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTTTAGCGGGAGGAAACACCGGGGAGCTGTTTTTT 2144 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCCGGCCGTTCTAGGGAGCTGTTTTTT 2145 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGAGAACGAGCAGTACTTC 2146 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGAAGGGACTAGCGGGAGTAAGGACAGATACGCAGTATTTT 2147 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTTACAGCGGGGGGAACACCGGGGAGCTGTTTTTT 2148 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGGATCTAGGGAATGAGCAGTTCTTC 2149 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTATATCCGGACCTTGAAGCTTTCTTT 2150 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCCGTCAGTGGGCTGATAGCAATCAGCCCCAGCATTTT 2151 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCGGGACTAGCGACGGATGAGCAGTTCTTC 2152 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCTGGGACAGGGGAGGGCTATGAGCAGTTCTTC 2153 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAAAGGACTAGCGGGAGCTGGGACCCAGTACTTC 2154 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTTCGTCAGGGGGGAGGGCCAGGGATACGCAGTATTTT 2155 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAGGGACTAGCGGGAGGGCCGAATGAGCAGTTCTTC 2156 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCGACAGGGCATTTATTCACCCCTCCACTTT 2157 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCCCCACCCGGGCCCAGTATGAGCAGTTCTTC 2158 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGGGGGGACAGGGCCCACTGAAGCTTTCTTT 2159 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAGAGAGAGGACGGTCTTCCTACGAGCAGTACTTC 2160 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGACTGGGCCTTCTTACGCAGTATTTT 2161 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCCGGGACAGGGTGAAGGGTACGAGCAGTACTTC 2162 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTACTAAGGGCCTACGAGCAGTACTTC 2163 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGGGGGACAGTAACACCGGGGAGCTGTTTTTT 2164 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGGGCCCATTGGGACCGAATCAGCCCCAGCATTTT 2165 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCCTGGGGGCAGCACAGATACGCAGTATTTT 2166 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCAAGTCGCCCATGGTTGGGACAGGGAAACACCGGGGAGCTGTTTTTT 2167 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCTGCAGGGGAGCGGAGCTACGAGCAGTACTTC 2168 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACGACAGGGGCCTTTATGGCTACACCTTC 2169 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGTCAACAGGGGGCGGTCAGCCCCAGCATTTT 2170 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGACAGGACAGGGGGTTTTCCTACGAGCAGTACTTC 2171 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCATTGTCTAGTAGCCACAATGAGCAGTTCTTC 2172 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGGGAACAGGGAGGGGGCTACACCTTC 2173 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTGGTCTGGCGCTCGCACAGATACGCAGTATTTT 2174 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGATTTAAGCGGGTGGAACACCGGGGAGCTGTTTTTT 2175 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCCTCCACGGGAGAGACCCAGTACTTC 2176 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCTAGCGCGTTCGAGCAGTACTTC 2177 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCTCCCGGCAGGGACAGGGCACAGATACGCAGTATTTT 2178 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGTGACCCGGGCCACTGAAGCTTTCTTT 2179 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTAAGCCCGACAGGGGGCGGTACGAGCAGTACTTC 2180 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGTGTCCCTAGCGGGAGTTCAAGAGACCCAGTACTTC 2181 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCGCCCAAATTCCGGGACTAGCTTCGTGGAGACCCAGTAC TTC 2182 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAACGGAGCTGGACTACGAGCAGTACTTC 2183 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGAGAGCAGGGAGGCGAGTGAAAAACTGTTTTTT 2184 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCCCGTTCGGTGAAGCTTTCTTT 2185 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAAGCCCAGGCGGGACCCAGTACTTC 2186 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGATCCAAGACTAGCGGGACCCGCC GCAGATACGCAGTATTTT 2187 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTGGAGAAGAGGGGCGGAGACCCAGTACTTC 2188 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCGGCAACGCGAGGAGCAATCAGCCCCAGCATTTT 2189 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAGACTAGCGGGGAGCACGCTACGCAGTATTTT 2190 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGAACACAGCAAACACTGAAGCTTTCTTT 2191 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGCCCCCAGTGTGAGGTTTCAAGAGACCCAGTACTTC 2192 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTACCCAGGGGCCGGGACTGAAGCTTTCTTT 2193 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAGCTGGGACTAGGGTCATTCAGTACTTC 2194 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCAAACTCTAAGTACGAGCAGTACTTC 2195 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGGACCGGGACAGGGGGGGACTTTT 2196 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGGGCTATCCTACGAGCAGTACTTC 2197 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCCCATCAGACAGTCTCATACACAGATACGCAGTATTTT 2198 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCCGAGACGGACACAGATACGCAGTATTTT 2199 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTCGCCGGGACCCCGGGGAGCTGTTTTTT 2200 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTTTTCCAGGGGGGCGCTGAAGCTTTCTTT 2201 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGATTATTAAGCGGCAGGGGGCGGGATGGCTACACCTTC 2202 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGTCGTGGCGGGCGGGCTGAACAATGAGCAGTTCTTC 2203 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTACCTACGGGACTAGCGTCAGACTCACAGATACGCAG TATTTT 2204 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGGGACAGGGGTAAGGGTTTATAGCAATCAGCCCCAGCAT TTT 2205 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGAACAGGGGCTCTAACTATGGCTACACCTTC 2206 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTCAGGGGGGGGCAAGTAGGGAACACTGAAGCTTTCTTT 2207 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTATCACAGTGCTCGCGGATCTAGCCAAAAACATTCAG TACTTC 2208 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTTTCGAGACGGACGCATCGGAAACACTGAAGCTTTCTTT 2209 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGAATCTTCAGGGATGAGGGCCGGGGAGCTGTTTTTT 2210 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTATCCAGACAGGGCAGCTATGGCTACACCTTC 2211 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGATACTACCCCCTCAGATACGCAGTATTTT 2212 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCGAGCGGCAAGAGACCCAGTACTTC 2213 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGAGTGTACCGGGATTCGGACGGAACAATCAGCCCCAGCATTTT 2214 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGACCTAGCGGGAGGGCTGAAAGGGGTCTTC 2215 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTGGGGGAGGCGGATCGTGGCCCTCTCAAGAGACCCAGTACTTC 2216 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAATGGGGACTAGCGGGAGAGGGGATACGCAGTATTTT 2217 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGCGCGGCAAAGTGGCTACACCTTC 2218 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTACAGGGGGGCTAGCACAGATACGCAGTATTTT 2219 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATGGGCGGGAGGAGCAGATACGCAGTATTTT 2220 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGGACTAGCGGGCTTTCGAATGAGCAGTTCTTC 2221 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGACCATATGGGACACCTAATAGCAATCAGCCCCAGCATTTT 2222 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACTCAAGGGGCAGCGAACACTGAAGCTTTCTTT 2223 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGAGCCGTACTGAAGCTTTCTTT 2224 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATTCAGGGGACGCTGGGGCCAACGTCCTGACTTTC 2225 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGTCGGAGTATACAATGAGCAGTTCTTC 2226 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTATGCGGGGTTCGGGGTTCGGAGAGACCCAGTACTTC 2227 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTACCCAGGGGGCGAGACGAGCAGTACTTC 2228 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAACGGGGAGAATTTACAATGAGCAGTTCTTC 2229 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAACCGGAGCTGGCTACACCTTC 2230 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGGTCCTAGCGGGCCGCTCGGAGAGACCCAGTACTTC 2231 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTACCCGTATCCGAGCAGTACTTC 2232 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGGGCGGGAGTAAGGCAGATACGCAGTATTTT 2233 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCACCGACAGCAATAATGAAAAACTGTTTTTT 2234 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAAGACCCGGGACTAGCGGAACCTACGAGCAGTACTTC 2235 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAGGGACTAGCGGGAGAGCCGGGGAGCTGTTTTTT 2236 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCCGGGAACCAGCCTCTAACTATGGCTACACCTTC 2237 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGCGACACCGGACTAGCCGGGGAGACCCAGTACTTC 2238 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAGCTGTGGTGGCAGGCTATGGCTACACCTTC 2239 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCAACGTTTTACACTGAAGCTTTCTTT 2240 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGGCCCTTGCGGGAAATGAGCAGTTCTTC 2241 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCAGACTCCGGAGTCCCGTACGAGCAGTACTTC 2242 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTCGGGGCTCGTCCTACGAGCAGTACTTC 2243 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGCCGGGGAAGCAAGCTACGAGCAGTACTTC 2244 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTCCGGGTGGGGGGAGGCAATCAGCCCCAGCATTTT 2245 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGAGTGCTAGCGGGAGAGCGGATACGCAGTATTTT 2246 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCACCCCGGGGGCAGGGTGACACTGAAGCTTTCTTT 2247 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGACCCGGACTAGCGGATCCCAGTTCTTC 2248 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGCGGGGGAGGGCCTCTCCAATGAGCAGTTCTTC 2249 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGGTCCCAAGCGTGGAGACCCAGTACTTC 2250 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGGGTGGGCGGACGGGAGTTATGAACACTGAAG CTTTCTTT 2251 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGAACTAGCGGGAGGCGAGCAGTACTTC 2252 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGGAGGGCCGACGATGAGCAGTTCTTC 2253 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAGGGGACATCTCTGGGGCCAACGTCCTGACTTTC 2254 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAAGGGGGGCTCCAATCAGCCCCAGCATTTT 2255 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCACGGACCGCCCTTGCTCGAGCAGTACTTC 2256 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAATCTCGACCGGGACAGGGACCAATGAGCAGTTCTTC 2257 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCCGAGGACGGCCGGAAAATGAGCAGTTCTTC 2258 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCAAGTCGCTTCGGGACAGGGATTATCCAAGAGACCCAGTACTTC 2259 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCTCTAAGGGAGGGGCAGTACTTC 2260 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGACCCGATGACTAGCGGGAGTTTCTATGAGCAGTTCTTC 2261 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGTCAGGAACGCGTGGCTACACCTTC 2262 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGGGCGGGATTTAGGAAGGTCCAACGAGCAGTACTTC 2263 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAGGACGGAACGGAACACTGAAGCTTTCTTT 2264 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGTCGGAGGGACTAACTATGGCTACACCTTC 2265 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTCGCAGGGAATTCAACAATGAGCAGTTCTTC 2266 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAGATCGAGGTTCAAGCGGTGAGCAGTTCTTC 2267 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCAGGGGGCTTCATGTTCTATGGCTACACCTTC 2268 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGAACAGCTCTAGCGGGGGGAGGTGAGCAGTTCTTC 2269 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCGAGAGACAGGCATCTTTCTACGAGCAGTACTTC 2270 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCAGACAGACCAAGTAGGGTCTTC 2271 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGGCTGACAGGGGAGGACACTGAAGCTTTCTTT 2272 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTCAGCTACGAGCAGTACTTC 2273 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAACGTCTTCGGCGTTGGGGGCCGGGGAGCTGTTTTTT 2274 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCTCGTGAGGGGTCGGGCACTGAAGCTTTCTTT 2275 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGAGGGGTCTCAGCCCCAGCATTTT 2276 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCTCTTGGGGAAGCGGGCTCCTACACCGGGGAGCTGTTTTTT 2277 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCAGGGGGCGACGCCAAGAGACCCAGTACTTC 2278 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCGGGTCAGGCCAACCCATTCAGTACTTC 2279 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCACATCCGGGACAACCCTACGAGCAGTACTTC 2280 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGAAAGGGACTACGAGCAGTACTTC 2281 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTACAGGGATCGATCAGCCCCAGCATTTT 2282 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCGAAGGGTGGAAAACACCGGGGAGCTGTTTTTT 2283 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGAGTAGATACGCAGTATTTT 2284 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCGGACAGGGGCCACTGAAGCTTTCTTT 2285 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGGCACGACAGAAGAAGCTTTCTTT 2286 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCGACGAGTGAGCCCCTACGAGCAGTACTTC 2287 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTATTAGCGGGAGGGCCTTCCGGTGAGCAGTTCTTC 2288 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCCGCCCTCCGGGCGCGGGAGTTATTGTGGGGCAAGAG ACCCAGTACTTC 2289 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGGCCAGACAACAGGGCGGACTGAAGCTTTCTTT 2290 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGAGGGACCTGGAACACCGGGGAGCTGTTTTTT 2291 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCGTCTAGGGTACAATGAGCAGTTCTTC 2292 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCGTCCGGGGGAGAGGAACACTGAAGCTTTCTTT 2293 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCCGATCGGGACAGGGGAACACCGGGGAGCTGTTTTTT 2294 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCAGGAAGCGCATGGGACTCCTCTAATGAAAAA CTGTTTTTT 2295 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTAGAGCGCGCAATGAGCAGTTCTTC 2296 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGCATTCAGGGGCGAGCAGTACTTC 2297 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTACCCCCCAAGACCACGTGGAGCAGTTCTTC 2298 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGGGGACTAGCCTACGAGCAGTACTTC 2299 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGCTCGCGATTGGGGAGGGCCTATTACAATGAGCAGTTCTTC 2300 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGAGTGGGAGACTAGCGGGAGAACCACTTATCTTC 2301 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTGGGGGGGGTGGGAAAAACTGTTTTTT 2302 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTGAACAGCGGGACAGGGGCAATGAGCAGTTCTTC 2303 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAAGGGGGCACCCCGACTGGGTATGGCTACACCTTC 2304 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGATCGACAGTTTACTACGAGCAGTACTTC 2305 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGGTAAAGCGGGAGTTAATCCCGGGGAGCTGTTTTTT 2306 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGACGGGACAGGCGGGGGGAATGAAAAACTGTTTTTT 2307 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCCTACAGGGGGTTTTGGGAGAGACCCAGTACTTC 2308 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGAGAGGGGCTACGAGCAGTACTTC 2309 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTACGACAGGGGGTGCTAACTATGGCTACACCTTC 2310 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGTACAGGGGGCTGGTGGCTACACCTTC 2311 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGGGGGGGACTCTGGAAACACCATATATTTT 2312 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGAGCGCCCACGAACACTGAAGCTTTCTTT 2313 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTATTTCAGGGGAAAGGGGTGAGCAGTTCTTC 2314 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGAGTGAGGGCGGGAGTCTCTACGAGCAGTACTTC 2315 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACGGGACCGACTACGAGCAGTACTTC 2316 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCGGGGGGCTACAATGAGCAGTTCTTC 2317 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGAACCCCCGGAAGGGCTCCTACAATGAGCAGTTCTTC 2318 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTGTACGCCGTGGCAATGAGCAGTTCTTC 2319 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCAAGATACGCAGTATTTT 2320 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCCAGACAGGGACCTACGAGCAGTACTTC 2321 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAAGAGAGGCGGCTCCTACAATGAGCAGTTCTTC 2322 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGAGGGGACGCTTGGCACCGGGGAGCTGTTTTTT 2323 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAAGCGGGACAGGGGGAGAAAAACTGTTTTTT 2324 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGAGGTCTAGCGGCTTGATTGGTGAGCAGTTCTTC 2325 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGAGCTAAGGGAGCCCCCCTACAATGAGCAGTTCTTC 2326 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATGACAGAACGACGAGCTCCTATAATTCACCCCTC CACTTT 2327 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGGGGAACAGGGCTCGACTCCTACGAGCAGTACTTC 2328 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGGGGCCTTTGGACTAGCCCGGGTAGCTC CTACAATGAGCAGTTCTTC 2329 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGGTCAGGGGGGACAGACCCAGTACTTC 2330 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCACTCAAGGACAGGACTTACCCCTACGAGCAGTACTTC 2331 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCTGGACTAGCGGCACAGATACGCAGTATTTT 2332 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCGACGGTTTACGAGCAGTACTTC 2333 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGAGTCGGCAGGGAGCAACACTGAAGCTTTCTTT 2334 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCATCCCCGACAGGGCCCAGCAGTACTTC 2335 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAAGCAGGGGGCGAGGACAGATACGCAGTATTTT 2336 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCTGGACAGGGTTTCGCCTACGAGCAGTACTTC 2337 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCACGAGAGGGACTAGCGGTTTTTATCCCTCCCTCGCTGGG GCCAACGTCCTGACTTTC 2338 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCGACGGACTAGCGGAACCTACAATGAGCAGTTCTTC 2339 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGCCCAGTACGGCGGAAATCAGCCCCAGCATTTT 2340 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGGACTAGCGGGTTACAATGAGCAGTTCTTC 2341 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGACCGGGACTAGCGGCCTACAATGAGCAGTTCTTC 2342 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCCGACAGGGGAGGAAATACGCAGTATTTT 2343 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGAGGGGGCTGGAAAACTGTTTTTT 2344 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTATGGGAGCTCCTACAATGAGCAGTTCTTC 2345 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAATCCCAGGGACTCGGCAGATACGCAGTATTTT 2346 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGACGGCGAGCTGGCAGTTCCAAGAGA CCCAGTACTTC 2347 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTCGGGACAGCACCTACGAGCAGTACTTC 2348 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGGGCGGACAGGGGAGGGAATCAGCCCCAGCATTTT 2349 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCAGGGGTGTAGGGACTGAAGCTTTCTTT 2350 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTATCCGGGAATAGCAATCAGCCCCAGCATTTT 2351 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCGGACAGGACTCCTACAATGAGCAGTTCTTC 2352 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAACCCACCGGGCGGGGGTACGAGCAGTACTTC 2353 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTCACAGGGAGTGAGACCCAGTACTTC 2354 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTCCGGCTAGCGGATCGTACAAATGAGCAGTTCTTC 2355 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAGAGACAGGCAACGACCACAGATACGCAGTATTTT 2356 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGTCGGGCAGATACGCAGTATTTT 2357 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGACGGACTAGCGGGAGGGCCGATGAGCAGTTCTTC 2358 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAGACATGAATCAGCCCCAGCATTTT 2359 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACTTACGACAGGGGGTAACACTGAAGCTTTCTTT 2360 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCTCATTGGGATTACCTACAATGAGCAGTTCTTC 2361 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGACAGGGTATGGACTGAAGCTTTCTTT 2362 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAGGGGTCACTCACAGATACGCAGTATTTT 2363 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTTTCGGCCCGAACACCGGGGAGCTGTTTTTT 2364 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACGCTGTCCCGGGACTAGCGGGCTCGACCTA CAATGAGCAGTTCTTC 2365 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGGACAGTAATCAGCCCCAGCATTTT 2366 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCCCCGGGACAGGCAGGTTCACCCCTCCACTTT 2367 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAAATGGGGATACGCAGTATTTT 2368 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATTACAGAATGTTTCACCCCTCCACTTT 2369 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCTTCACCGGGACAGGGGCCCAATGAGCAGTTCTTC 2370 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGAGTCGAGAGGGCGGGACTCTACAGATACGCAGTATTTT 2371 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAGGGAGGACAGGGAGGGAACGAGCAGTACTTC 2372 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAATTGCGGGGAGCCTACGAGCAGTACTTC 2373 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGCTTCAGGGGAGGAATCAGCCCCAGCATTTT 2374 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTAAGTCCCAGCTCAATCAGCCCCAGCATTTT 2375 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGCGGGAGGGGGTTCTCGGCAATGAGCAGTTCTTC 2376 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTCGACCTAGTCACCGGGGAGCTGTTTTTT 2377 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCTAGTGGTTCCGGGTACAATGAGCAGTTCTTC 2378 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCCCCCCCAGGGAAGGCCACTGAAGCTTTCTTT 2379 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGACACGGGACTAGCAGTTACGAGCAGTACTTC 2380 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTGGGGGGGACAGGGGTTGGACGACTATGGCTACACCTTC 2381 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAGTCCCCCAGGGGCAGAGAGACCCAGTACTTC 2382 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGGGCCTACCCGATCCGGAGACCCAGTACTTC 2383 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCAGGGAATCTTAACTACTCTTACTACGAGCAGTACTTC 2384 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGACCGGGACAGGGAAAGGCTACACCTTC 2385 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTGAGATGTACTACGAGCAGTACTTC 2386 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTAGCATCGGCGCCTCGGGGTCGGATACGCAGTATTTT 2387 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCCCGTACTAGCGGAATCCCCTCCTACA CAGATACGCAGTATTTT 2388 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCAGGGCCGGGAGTCGATCAGCCCCAGCATTTT 2389 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCCAGGGTTCCTACAATGAGCAGTTCTTC 2390 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGGGTTGTGACTAGCGGGAGTAACAATGAGCAGTTCTTC 2391 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAACTCGGACGGGAGCTCCTACAATGAGCAGTTCTTC 2392 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGACCCGGGACTGCTCACCGGGGAGCTGTTTTTT 2393 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGGGTCGGCCTACGAGCAGTACTTC 2394 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACGGGACAGTAACCTACGAGCAGTACTTC 2395 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTATCTACAATGAGCAGTTCTTC 2396 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAACGAAGATCAGTAGCACAGATACGCAGTATTTT 2397 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGATGGGAGGAGAGGGTCCTACAATGAGCAGTTCTTC 2398 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCCCGGACAGAGCTACGAGCAGTACTTC 2399 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTATCTGGGGGCACTGAAGCTTTCTTT 2400 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGGGGGACCTGGTGCTGGCTACACCTTC 2401 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTCGGGGACGGGGAGATGAGCAGTTCTTC 2402 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGAGAGGGCACCGGGGAGCTGTTTTTT 2403 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGCTTACGGGACTACTACAATGAGCAGTTCTTC 2404 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCTACACGATCCACTATGGCTACACCTTC 2405 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGTTTCCACCGGGGAGCTGTTTTTT 2406 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCCCACTGCAACTAATGAAAAACTGTTTTTT 2407 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAAGCGGGAGATACAATGAGCAGTTCTTC 2408 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGAAACAGCTACGGAGACCCAGTACTTC 2409 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATGAGGACTGGGGGTACAATGAGCAGTTCTTC 2410 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCTACGGGGCCTCCTACAATGAGCAGTTCTTC 2411 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATGGCAGACCCTGCCTTTCTCTGGAAAC ACCATATATTTT 2412 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTCGGGGGAACACTGAAGCTTTCTTT 2413 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTCGGGACTAGCGGAGGCGGGGGCAATGAGCAGTTCTTC 2414 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATTTAGGGTCCAAAAACATTCAGTACTTC 2415 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGTCCCGGGACAGGGGTACGAGCAGTACTTC 2416 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAAGCGGTCAGCTCCACTACGAGCAGTACTTC 2417 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAGGGGGTGGGGAGACCCAGTACTTC 2418 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCGGACAGATCAATCAGCCCCAGCATTTT 2419 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGCCCCCAACTCTGGAAACACCATATATTTT 2420 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCGAGATCAGGCGAGAACGATTACGAGCAGTACTTC 2421 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCGAGCTTGCACGGGGCACTGAAGCTTTCTTT 2422 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGGGTACCGGGCTAGCGCCCAAGAGACCCAGTACTTC 2423 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGCCCTCCAAAATCAGCCCCAGCATTTT 2424 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACTAAATCTGGGGCCAACGTCCTGACTTTC 2425 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTGGGGCCGTCTATGGCTACACCTTC 2426 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCGATGGGCGGGACCTTGCTGGGCACTGAAGCTTTCTTT 2427 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTACCCCACAGGGGTCACAGATACGCAGTATTTT 2428 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGACCGGGAGGGCCGATCAATGAGCAGTTCTTC 2429 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGGAGACCGGGACTTCAACAATGAGCAGTTCTTC 2430 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGGGGACAGAGCTCCTACAATGAGCAGTTCTTC 2431 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTGCCTTTAGCGGAGAGAAACATTCAGTACTTC 2432 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGTTTCGCTGGGGAGTAATGAAGCTTTCTTT 2433 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTATAGCACAGGGGGCGACTATGGCTACACCTTC 2434 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGACGACTAGAGTTTGGCGAGCAGTACTTC 2435 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGATCTAGGGTATGGCTACACCTTC 2436 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATCAGGGCAGTTTAATCAGCCCCAGCATTTT 2437 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCGAACTCTCTTGGAGACCCAGTACTTC 2438 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTGAATAGCAATCAGCCCCAGCATTTT 2439 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAACGCGAGCCGCGGGAGCAAATGAGCAGTTCTTC 2440 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACAGGGGGGTTCCTGGCTACACCTTC 2441 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCAACCGGTTCGGGGACCCCCTACGAGCAGTACTTC 2442 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCGGGACCACCTAAGATCTACGAGCAGTACTTC 2443 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTGGGGGTTGAGAATTCACCCCTCCACTTT 2444 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTAGCGGGAGTGACTGGGGCCAACGTCCTGACTTTC 2445 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCACAGGGATTGATCAGCCCCAGCATTTT 2446 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGGGACTATCTACAATGAGCAGTTCTTC 2447 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCGGACAGTATAGCAATCAGCCCCAGCATTTT 2448 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCACGAGGTCCTCTAATGAAAAACTGTTTTTT 2449 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAACCTTGGTGCTATCGGGGCCAACGTCCTGACTTTC 2450 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCGGACCTTCCCGACTCTGGAAACACCATATATTTT 2451 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGTCCGGACTGAAAAACTGTTTTTT 2452 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCTTGGGAAGAGACCCAGTACTTC 2453 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTAGACAGGGGGGTTCGAATGGCTACACCTTC 2454 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATGGGGACAGGGGGCCCGGAACACTGAAGCTTTCTTT 2455 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGGAGGGTTCGGGATGTCGGGCGAGCAGTACTTC 2456 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCTCGGTTGGAGTAGGAGGAACCGGGGAGCTGTTTTTT 2457 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCGGGACAGCCTAAAAGGGTACTTC 2458 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCGGGACTAGTGAAAACCGGGGAGCTGTTTTTT 2459 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCACCACCGGGACAGGGGCGCTCGGGGCCAACGTCCTGACTTTC 2460 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCCGAAACGGACTAGCGGGAGGGCCTTCCTACGAGCAGTACTTC 2461 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTATGGCAGAGACACAGATACGCAGTATTTT 2462 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGACTCAAGGGACCCGAGCTTTCTTT 2463 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTCGGGGGGGATACAATGAGCAGTTCTTC 2464 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACGACATTAGGCTCTGGGGCCAACGTCCTGACTTTC 2465 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAGCCGGGAGCGAGCAGTACTTC 2466 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTACCGAGGGAAATCAGCCCCAGCATTTT 2467 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGAGACGCTAGCGGGCAACAATGAGCAGTTCTTC 2468 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGAACAGGCGAGGACCGGGGAGCTGTTTTTT 2469 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGGCAGAAACCTACGAGCAGTACTTC 2470 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGACGACTTCGGCGGGAGTTCCTACGAGCAGTACTTC 2471 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGTCCTAGCGGGAGGGGTCAATGAGCAGTTCTTC 2472 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGGACCGGAGCGGGAGACCCCTACGAGCAGTACTTC 2473 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCCTGGACTGTCTCGAACACCGGGGAGCTGTTTTTT 2474 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGAGTGCCCTGGCTGGGGCTTTCTTT 2475 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGGACCTGGACAGGGGGACTATGGCTACACCTTC 2476 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATGCAGACTCGAACACCGGGGAGCTGTTTTTT 2477 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCATCATTATGGGGGCTGAAGCTTTCTTT 2478 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCCTGGACTAGCGGGAGGGCCGACTTT 2479 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCTAATGATATCAGGGGGACAGCAGTTCTTC 2480 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAAGACTAGGGGTTAGAGAGCAGTTCTTC 2481 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCCAGACAGATACGCAGTATTTT 2482 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCTAGCAGCCCAGTGCCCGGGACAGGGGAAGGGACCGGGGA GCTGTTTTTT 2483 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGGTCCTGTTTCGGCAGGCCTAAATTCACCCCTCCACTTT 2484 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCCATACCGGGACAGGGGCCTACGAGCAGTACTTC 2485 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTCGGTCAGGGTTTTAGTGAGCAGTACTTC 2486 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGGATGGACAGGGGAATACGAGCAGTACTTC 2487 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCTCCCCGATAGGAGGCGGGGTTAATAACACT GAAGCTTTCTTT 2488 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAAGAGACCAAAGAGAACTATGGCTACACCTTC 2489 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCATTGGGACAGGACACTACGAGCAGTACTTC 2490 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGGTTCTAGTCTCGGTACGCAGTATTTT 2491 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTCATTGGGACTAGCGTATACAATGAGCAGTTCTTC 2492 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGTGGCTAGCCAGGGGCTCATATAATT CACCCCTCCACTTT 2493 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGGCAGGGGGCATGGTCAGCCCCAGCATTTT 2494 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTGATGTACAGAGCAATCAGCCCCAGCATTTT 2495 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGAGCCTTCCTGGAAACACCATATATTTT 2496 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTCAAGCGAGGATTAAACAAGAGACCCAGTACTTC 2497 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGTGGAGGGGAATGAGCAGTTCTTC 2498 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGAGGGACTAGCGGGAGGTGAGCAGTTCTTC 2499 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGCCGACCTCAGGGGGCGGATCACCCCTCCACTTT 2500 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGCGGCACAGGGGGCAGGGCAGCCCCAGCATTTT 2501 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCAGAAGGAGGGTTTGACCCAAATCAGCC CCAGCATTTT 2502 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGTCTACCGGGACAGGGCTCAATGAGCAGTTCTTC 2503 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCCCGCCAGGGGGACGAGCAGTACTTC 2504 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCCTCCGTGGGAGCGGGAGTTGTAGAGACCCAGTACTTC 2505 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCACGTTGCGGTTACCGGGGAGCTGTTTTTT 2506 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGTGTGGGGGTCTAGCACAGATACGCAGTATTTT 2507 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCTCGGACAGGGAAGACGGTCAATGAGCAGTTCTTC 2508 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGACCCTGGACATTACCTACGAGCAGTACTTC 2509 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCATAAACCGGAACACCGGGGAGCTGTTTTTT 2510 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCACCTCGGGAGTTTTAAAGACCCAGTACTTC 2511 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAGAGGACAGATCCTATAGCAATCAG CCCCAGCATTTT 2512 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCAGCGGGAGCTACACCGGGGAGCTGTTTTTT 2513 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCACGGATGGCCACAGATACGCAGTATTTT 2514 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCACCGAGGACTAGCGGGAGTTACACCGGG GAGCTGTTTTTT 2515 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGACATTTGAGGGGTGTCTCCTACGAGCAGTACTTC 2516 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTGAAACAGACACAGATACGCAGTATTTT 2517 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCACTCAGGGGTTCAGCACTGAAGCTTTCTTT 2518 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGAGCGGCGGAGGGGATCAGCCCCAGCATTTT 2519 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGATTAACCGGGACAAGTCTTAGCGAGCAGTACTTC 2520 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACGGGTCAGGGGGAGAGACCCAGTACTTC 2521 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAAGAGAGGGCGTGGGGAATGAGTGAGACCCAGTACTTC 2522 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCAACACTGACAGGCAACGAGCAGTACTTC 2523 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACAAGAGTGGGGGGGTCTCAAGAGACCCAGTACTTC 2524 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCGCCGGGACTTTCTAACTATGGCTACACCTTC 2525 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCGTAACCCCGGGACAGGGGTACGAGCAGTACTTC 2526 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAAGTCGTATCTACAATGAGCAGTTCTTC 2527 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAAATTTACGGTGTTGAACACCGGGGAGCTGTTTTTT 2528 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCATAACCTCTCGCCCGTACAATGAGCAGTTCTTC 2529 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGAGGACGGGGCCAACGTCCTGACTTTC 2530 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAATGCAGTGGGCACAGATACGCAGTATTTT 2531 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCACGTAGCAGATACGCAGTATTTT 2532 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCTTACAGGGCCAGGGCTACACCTTC 2533 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGATGGGACTAGCGGATACGAGCAGTACTTC 2534 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCAGGACTAGCGGGAGGGCCCAGCGGCCAACACAA TGAGCAGTTCTTC 2535 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGTGACAATGAGCAGTTCTTC 2536 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGCGCCCGGCGGGGAGCTGTTTTTT 2537 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGATGGAGGGGTGGGAGAGAGTGAGCAGTTCTTC 2538 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGAACCCACAGGGGTGGACACCGGGGAGCTGTTTTTT 2539 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAACGCCTCCAGTTCTTC 2540 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGAGACTTACCCTTGATGGAGATACGCAGTATTTT 2541 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCCGCAAAAACAGGGAGCACCGGGGAGCTGTTTTTT 2542 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAAGAAGGTAGCGGGAGGCAAGAGACCCAGTACTTC 2543 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCGAGACACCTCGGAGCAGTTCTTC 2544 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTGGGGTAGCGGGATGGACCGGGGAGCTGTTTTTT 2545 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGCAGACTAGCGGGGGGGTACAATGAGCAGTTCTTC 2546 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGAGGGGGTGGAACACCGGGGAGCTGTTTTTT 2547 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAATGGGTGGGGGGAATGAGCAGTTCTTC 2548 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATCTAGCGGGAGTAAACAATGAGCAGTTCTTC 2549 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGGTCTCGATGGACGACGGTGAAAAACTGTTTTTT 2550 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCACAGGGGCGGCAGCAAGAGACCCAGTACTTC 2551 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCAGGGACAGGGAAGGGCCGAGAGACCCAGTACTTC 2552 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGAGGGGGGCTCCTACAATGAGCAGTTCTTC 2553 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCCGGGACAGGGGACTCTACAATGAGCAGTTCTTC 2554 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTATTGCTTAATTGAAGCGGGAGAATGTGAGCAGTACTTC 2555 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTCCCTACAGAGATACGCAGTATTTT 2556 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGACAGGCGTTCCTACACCTTC 2557 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAAGACCGGACAGGGTGGCAATCAGCCCCAGCATTTT 2558 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACGGTCCGGGAGAGACCCAGTACTTC 2559 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCCGGGACAGCGGATGGGGCCCGAGCAC AGATACGCAGTATTTT 2560 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTAACGAGCTCCTACAATGAGCAGTTCTTC 2561 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCGGGAAGGGCAGTTCGAGCAGTACTTC 2562 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTGGATAGGGACAGCCAAAGCTTTCTTT 2563 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCAGGACTAGCGATGAACACCGGGGAGCTGTTTTTT 2564 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCTAGCCGGCGATACGCAGTATTTT 2565 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGGTCAGCACAGATACGCAGTATTTT 2566 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCATTACGGTACCCTGAACACTGAAGCTTTCTTT 2567 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCCCGGGTAGCGGGATATTACGAGCAGTACTTC 2568 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGGGACACCCAATCCGAGCAGTACTTC 2569 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGATTACTAGCGGGCCTTACGAGCAGTACTTC 2570 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCTGACCCAGGGAAATAATTCACCCCTCCACTTT 2571 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTGAGGTTCCGGGAGGGGTTTATGGCTACACCTTC 2572 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTAGCGGGAGGACCTACGAGCAGTACTTC 2573 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATGGGGAGCCCTGGAGCAGTACTTC 2574 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATGCCGAACACTGAAGCTTTCTTT 2575 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGGGACTAGCGGGAGATCCCTTC ACAGATACGCAGTATTTT 2576 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGTGGGGCTACAAGAGACCCAGTACTTC 2577 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCCAGGGGGCTTAGTTCCGATATG AACACTGAAGCTTTCTTT 2578 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAAGGTGGGCTAGCGGGAGGGCCTAAG TCCAAAAACATTCAGTACTTC 2579 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGTCAGGGAGAACACCGGGGAGCTGTTTTTT 2580 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAAGACTAGCCCCCCAAGAGACCCAGTACTTC 2581 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGAGTGGGGGCCCCAGCATTTT 2582 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCCCAGGGGAGCGACTCCTACGAGCAGTACTTC 2583 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTTTGGGTTCCTACAATGAGCAGTTCTTC 2584 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAATTTCAGGGCGCAGGTTATGAACACCGGGGAG CTGTTTTTT 2585 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCCAGCTCCGGGACAGGGTTTAACTATGGCTACACCTTC 2586 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGGTCGGAACAGTATAAACTATGGCTACACCTTC 2587 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTCGCGGGGAGGGCACTGAAGCTTTCTTT 2588 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGAAGCGGGAGGGCCGGCCGGGGAGCTGTTTTTT 2589 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGGGGACAGTCCTACGAGCAGTACTTC 2590 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACGAGCGGGAGGGAGCACAGATACGCAGTATTTT 2591 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTAAACAGGTTCTTAGCAATCAGCCCCAGCATTTT 2592 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTGCCTCAAACAGGGGTGAAAGTGAAGCTTTCTTT 2593 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCCGTCGCCACCGGGGAGCTGTTTTTT 2594 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCGCACGATCAGGGGGCGGCGACCTACGAGCAGTACTTC 2595 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAGCCGGGACAGACCTTTTCACAGATACG CAGTATTTT 2596 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCCTTGGGAACAGGGGTATGGGGTGAGCAGTTCTTC 2597 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTTGGACGAGGGGACCAAACTCCTACGAGCAGTACTTC 2598 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGTACTGGCCAATGAGCAGTTCTTC 2599 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTATCGGCTTAGCTCCTACAATGAGCAGTTCTTC 2600 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGACGCGGGGGAGCGAGCTTTCTTT 2601 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTCGGAGGGACAAGACTGAACACTGAAGCTTTCTTT 2602 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTGGGACTAGATACGCAGTATTTT 2603 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAAACCATGGAGTCAGGGATGGATTAACTA TGGCTACACCTTC 2604 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGGGACGAGCTCCCAAGAGACCCAGTACTTC 2605 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGCGGGACTAGCGGGCACGAACACCGGGGAGCTGTTTTTT 2606 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCATCGGACTAGCGGGAGCCATGAGCAGTTCTTC 2607 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGAAGGCGAGCAGCCCCAGCATTTT 2608 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCAGACCGGGACGGCACTGAAGCTTTCTTT 2609 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGGGGTCGGGGATACTAACTATGGCTACACCTTC 2610 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGGGACCATCCTACGAGCAGTACTTC 2611 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTAATTGGGACAGCCAAGAGACCCAGTACTTC 2612 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGAAACAGGGTGCAACTAATGAAAAACTGTTTTTT 2613 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTTACCTCCCGGGCGGGACAGGTTATGGCTACACCTTC 2614 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTATCGGGGGGCGAGCAGTACTTC 2615 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGGGGGATGGAGCGGGAGGACCAAGAGACCCAGTACTTC 2616 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCCTACTAGCGGTGAATACAATGAGCAGTTCTTC 2617 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGGAGCCAGGGATCATTATGAAAAACTGTTTTTT 2618 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCCCGGACTGAGAGCTCCTACAATGAGCAGTTCTTC 2619 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCCACCCCAGCGGGAGGGACCTACAATGAGCAGTTCTTC 2620 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGCGGGAGCAGGGCAATGAGCAGTTCTTC 2621 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGGGGGGACAGGGGGGGCAATGAGCAGTTCTTC 2622 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAGCAGGGGACCTTATGGACAGATACGCAGTATTTT 2623 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCATTGTTAACAGGGGTAAACTATGGCTACACCTTC 2624 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCATTCGGACAGGGCCTAACACAGATACGCAGTATTTT 2625 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGTTTATTTAGGCAGTCAAGAGACCCAGTACTTC 2626 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCCAGGGCCAACCCAACAATGAGCAGTTCTTC 2627 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTCAAACAGACAGACACAATGAGCAGTTCTTC 2628 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTTGCGGACTAGCGGGCCCTACAATGAGCAGTTCTTC 2629 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATCAGAAGCTCCTACGAGCAGTACTTC 2630 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAAGGGGGCGGGGGAACGCAGTATTTT 2631 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCGAGCGGGAATACAATGAGCAGTTCTTC 2632 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCATGGACTAGCGGGAGTATACGAGCAGTACTTC 2633 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGCTACGGTTAATTCACCCCTCCACTTT 2634 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCTCGGGCTATGGCTACACCTTC 2635 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATTTCCGGGGGGCAAGTACATTGGATTC ACCCCTCCACTTT 2636 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTAGAGGCCCCGGGAATTCACCCCTCCACTTT 2637 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCGAGGCACCTATGGCTACACCTTC 2638 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGACTACGGGACAATGAGCAGTTCTTC 2639 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAGCACGGGACAGGGGGTTACCATCGTTCTTC 2640 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGATGATATATACGCGGGCTACACCTTC 2641 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTCAAGTAGCGGGAGGGCGTCAAGATACGCAGTATTTT 2642 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTGACGAATCCAGGGGGCTCCTACGAGCAGTACTTC 2643 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTAACCGACAGGGTCCCGAGCAGTACTTC 2644 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGGAGGGGCCGGGACTCTATACAATGAGCAGTTCTTC 2645 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGTCCACAGGAGCCAGGAATCAGCCCCAGCATTTT 2646 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCGACTTACCGGGGAGCTGTTTTTT 2647 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTATGGGACAGGAGGAACACTGAAGCTTTCTTT 2648 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTGTCGGACTGGGCCGGGGAGCTGTTTTTT 2649 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCCTCCAGGCGGACACCGGGGAGCTGTTTTTT 2650 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGAGTAGCAACTCACAGGGCGGAGAAAAACTGTTTTTT 2651 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAACGAACGGGGGACCTATAATTCACCCCTCCACTTT 2652 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGTACTAGATCAGCCCCAGCATTTT 2653 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTTGGTATGAACACTGAAGCTTTCTTT 2654 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAGTTTATGAACACTGAAGCTTTCTTT 2655 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTCCGAACACTGAAGCTTTCTTT 2656 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGATCAGCCCCAGCATTTT 2657 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCAGGACAGGGAACCACCATATATTTT 2658 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGGACAGGCCGAACACTGAAGCTTTCTTT 2659 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGGGGCAGAGACCCAGTACTTC 2660 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCCCTGGACAGCCGGGAGCACTGAAGCTTTCTTT 2661 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTCGCTACAGCACTGAACACTGAAGCTTTCTTT 2662 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCCTTACCGGGACAGGGGGGTTAGAGGTTAG AAGCAAGCCCCAGCATTTT 2663 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGGTTCACCAGATACGCAGTATTTT 2664 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGGCACAGGGTACTACGAGCAGTACTTC 2665 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGTGGCAGGAAGCACAGATACGCAGTATTTT 2666 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTGGACAGGGGTTCCTACGAGCAGTACTTC 2667 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGCAGGGGGCAATTGGCAATCAGCCCCAGCATTTT 2668 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTGAGGGACCGAACCTACAATGAGCAGTTCTTC 2669 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAGAAACGGGGGGAACCGGGGAGCTGTTTTTT 2670 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCTCGACAGGAGATCTACTATGGCTACACCTTC 2671 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCATTCTATTCCCGGGACAGCCGAGCTACGAGCAGTACTTC 2672 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGCTTACGACAGGGTCTACGAGCAGTACTTC 2673 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAAGGGACTAGCCGAATATGAGCAGTTCTTC 2674 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCACTAGGGGGTCATCCTACAATGAGCAGTTCTTC 2675 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATACAGGGCCAAATCAGCCCCAGCATTTT 2676 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCACGGGACAGGGGTACACTGAAGCTTTCTTT 2677 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCAGACAGGGGCGGAGCACAGATACGCAGTATTTT 2678 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGACGGACAGGGCTTGTTCTATAATTCACCCCTC CACTTT 2679 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCGAACACAGGGGAATCAGCCCCAGCATTTT 2680 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGTGAGACTAGCGCTAAAGAGACCCAGTACTTC 2681 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAACTAGCGTTAGCACAGATACGCAGTATTTT 2682 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAACTCAGGCAAAGAGACCCAGTACTTC 2683 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGTAGTATCGGTTCCAGGGGATTTTCAGATACGCAGTATTTT 2684 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGAGTGTACGGGGAATCAGGAACACTGAAGCTTTCTTT 2685 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCGCGGACAGGGGAAAAAACTGAAGCTTTCTTT 2686 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCGCTTAGTGGGGACTAGCGGGAGAAGCAC AGATACGCAGTATTTT 2687 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGAGGCAGCTACGAGCAGTACTTC 2688 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCCATGCTTCTCCGGGACAGGGTCCCGCAGATACGCAGTATTTT 2689 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCTCCGCGGGGTGGAACAATGAGCAGTTCTTC 2690 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCCACACAGATACGCAGTATTTT 2691 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTACCAGAATTCACCCCTCCACTTT 2692 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCGCCTGGACAGGGGGATGGCGGAGCTCCTACAA TGAGCAGTTCTTC 2693 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTAGCGGGAGGGAGCACAGATACGCAGTATTTT 2694 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGGGGAAGTATCAGCCCCAGCATTTT 2695 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGCCGGGACAGGGGATCTACAATGAGCAGTTCTTC 2696 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTCTTCTCCGGGACACACTCCTACGAGCAGTACTTC 2697 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACTACTTGGCAGGGGGCCCCCTACAATGAGCAGTTCTTC 2698 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGTCCGACGACACCGGTACCAAGAGACCCAGTACTTC 2699 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGGAGGGCCGATAGGACAGGGCGGGATCCAACTGATACG CAGTATTTT 2700 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGACAACGGGGCGGGAGCAGCTATGAGCAGTTCTTC 2701 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCACAAGGACAGGGGGCGGGCTATGGCTACACCTTC 2702 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGCCGCCGGGACAGGGCTGACTGAAGCTTTCTTT 2703 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTGGGCAGAATCAGCCCCAGCATTTT 2704 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGATTTTAGAGGACTGAACACTGAAGCTTTCTTT 2705 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGGGGGTTGGCGAACACTGAAGCTTTCTTT 2706 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGTACGGGCCGGGGGATACGCAGTATTTT 2707 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCGGACTAACTCGGTACGAGCAGTACTTC 2708 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTGGGACAACCCCTCCTACAATGAGCAGTTCTTC 2709 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTGCGGGACAGGGAGGCAATGAGCAGTTCTTC 2710 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGGGGAAATGAAAAACTGTTTTTT 2711 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCGGGAAACAATCAGCCCCAGCATTTT 2712 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATCATACAGCGGGGACAACTTC 2713 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGTCGGTCTAAGGGGCTTTGGCTACACCTTC 2714 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCCATCGCGTCAGGGAAAGAGACCCAGTACTTC 2715 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTCAAGCATGGGCAGGTGAGCAGTTCTTC 2716 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGTTGGGCGGATCTACGAGCAGTACTTC 2717 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCTCGAGGACAGGGTGACGAGCAGTACTTC 2718 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTTGGCAGGGCGCGATGAGCAATCAGCCCCAGCATTTT 2719 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCCGGGTATTAGAGCTGAAAAACTGTTTTTT 2720 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGGGACCGGGGAGCTGTTTTTT 2721 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGTTTTTGGAGCCCCAGCATTTT 2722 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTCGCCAAGAACACCGGGGAGCTGTTTTTT 2723 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTAGTAAGCTCTGGAAACACCATATATTTT 2724 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATTAACTCCCTCCGGTGAGCAGTTCTTC 2725 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAAGCCCCTAGCGGGAGGAGGCAATGAGCAGTTCTTC 2726 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTACGGGACAGGGGGCGGATGGCTACACCTTC 2727 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCCACTGGGCTAGCGGGTTTCTCCTACGAGCAGTACTTC 2728 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGACTAGCGGGAGGCCGGCATGAGCAGTTCTTC 2729 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTACAGGGACGAAGAGGCTACACCTTC 2730 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGAGACTAGCGGGAGCGAGCAGTACTTC 2731 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTACCCGCGGCGTCGTGGCGGGAGGGACTC TACAATGAGCAGTTCTTC 2732 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCGAGGCAGGCCCTGGGGCCAACGTCCTGACTTTC 2733 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCTGTAAGCGGAGCATACAATGAGCAGTTCTTC 2734 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACGAGACAGGGATCGACCTACGAGCAGTACTTC 2735 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGGGACTACAGGAGACCCAGTACTTC 2736 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTACTTGGCAAGCCTAATGAAAAACTGTTTTTT 2737 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCAAGCAGCAGGGCGCGGGAGATCTACAATGAGCAGTTCTTC 2738 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAATGGACAGGGAAATTCTAAGCCCCAGCATTTT 2739 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAAGGGAGGACACTGAAGCTTTCTTT 2740 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCGCAGTTTCCGTGGCACAGATACGCAGTATTTT 2741 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGCAGAAACAGTGAACACTGAAGCTTTCTTT 2742 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGGGGGTTGATAATGAGCAGTTCTTC 2743 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGACTTGGGACTAGCGGGAAAGGCCGGCGCC GAGCAGTACTTC 2744 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGTAACCGGCGCCGAGCAGTACTTC 2745 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGATGGCGGGAGGCCCAATCAGCCCCAGCATTTT 2746 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGACCCCTTAGCGGGAGGGCCGAGGGCACAGAT ACGCAGTATTTT 2747 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGAGGAGGGGACATATAACACTGAAGCTTTCTTT 2748 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGTTGAAGGGATTGGAAACACCATATATTTT 2749 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAAGGACTAGCGGGAGGACTGTATACAATG AGCAGTTCTTC 2750 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCTAGTGGTTTGGTTACAGAGAATCAGCCCCAGCATTTT 2751 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGATACAGGGTTCGAGACCCAGTACTTC 2752 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCGTCCCGGGACAGGGTTTTCTACGAGCAGTACTTC 2753 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCGCCGCCGTCCGGGACGCCCTCCCCTACGAGCAGTACTTC 2754 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGATTTTACGGGCCGGCAGTTCTTC 2755 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGATGGGACTAAACCTAGCACAGATACGCAGTATTTT 2756 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGATTCAAGGGGGGCCAAAAACATTCAGTACTTC 2757 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTACTGGGCTCTGGAAACACCATATATTTT 2758 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGTAGGCGGCGCCGGGGAGCTGTTTTTT 2759 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCCCAGATTACAGACTAGCGGGAGAA AACGATGAGCAGTTCTTC 2760 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCTACCTCAGGTCGGGGGGATCAGCCCCAGCATTTT 2761 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGACGAGCTGGTCGAGATGGTGAGCAGTACTTC 2762 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGATTACACAGATACGCAGTATTTT 2763 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCAGGGCGCGGGAGATCTACAATGAGCAGTTCTTC 2764 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTCACCACTGGGACCTAACGAGCAGTACTTC 2765 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGTAAGCACCGGGGAGCTGTTTTTT 2766 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAGGGACAGGGGGCTGCGGAAGCTTTCTTT 2767 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAACAGGGCTTTCATATCAGCCCCAGCATTTT 2768 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTGGAGACAGCGGAAAACATTCAGTACTTC 2769 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGTGGTGCTGGCGAGCAGTACTTC 2770 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGCGGGGGCCAAAAACATTCAGTACTTC 2771 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGACCCGGGACTAGTCTCCTACAATGAGCAGTTCTTC 2772 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCCCAAAGTAGCGGCACCGGGGAGCTGTTTTTT 2773 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGGGCAGCGTTCGGGCGGCTCCTACAATGAGCAGTTCTTC 2774 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGATTCGGACGGGCCACAGATACGCAGTATTTT 2775 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCCCTTTAGACAGGCGGTAACTTTCTTT 2776 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGGTGGTGAGGGCGGGGGTAGCAATCAGCCCCAGCATTTT 2777 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCTGCTCTCGGAACTTACCCTACAATGAGCAGTTCTTC 2778 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGATCCTCCCCCGACTATGGCTACACCTTC 2779 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGGGGGGTACAGGAACACTGAAGCTTTCTTT 2780 91-TL101-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGCCGTGACTCACTACGAGCAGTACTTC 2781 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCTGCGGCCATGGAAGCTTTCTTT 2782 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTACGACTCAGGGGGGGCACGAGCAGTACTTC 2783 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGTGGGGGGAGAAAATCAGCCCCAGCATTTT 2784 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGGAGTAGAAGCCTACGAGCAGTACTTC 2785 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTTGGGTCTAGCGCCTATGAGCAGTTCTTC 2786 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGACCCGACCTCAATGAGCAGTTCTTC 2787 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGCCCAACCGGGACAGGGGGATGAAAAACTGTTTTTT 2788 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCAAAATGAAAAACTGTTTTTT 2789 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTCGGGACAGGGCCGTTTTGGCTACACCTTC 2790 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGACTAATTCTCCGAGGGGATCAGCCCCAGCATTTT 2791 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTCTACAGGGGATATGGCTACACCTTC 2792 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCATGGGGGATCGAACACTGAAGCTTTCTTT 2793 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGATGAGGACCAGAACACCGGGGAGCTGTTTTTT 2794 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGGAGGGGAAGAGACCCAGTACTTC 2795 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAACGAATCAGGCTTCTGCGCAGTATTTT 2796 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAGGAAGGATGGGGGCGCTCCTACAATGAGCAGTTCTTC 2797 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTACGGCCGAACACTGAAGCTTTCTTT 2798 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGATCCCCGCGAAGGGTTCTATAGCAATCAGCCCCAGCAT TTT 2799 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCCGAGGCGGGGCAGGCAATGAGCAGTTCTTC 2800 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGCACCCGGGACGTACACTGAAGCTTTCTTT 2801 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGCGCTAAGGGGGGGCCTGCCTGGCTACACCTTC 2802 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCTCCAGGGACGGGAACTCCTACAATGAGCAGTTCTTC 2803 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGGGGAGCTCCTACAATGAGCAGTTCTTC 2804 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCTAGGGGCCGAGCGGGTGGATGAGCAGTTCTTC 2805 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCGACTAGCGGGGCTACCAATGAGCAGTTCTTC 2806 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCACCCCCAGAGGGACCTTCACGTACAATGAG CAGTTCTTC 2807 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCATCCAGGGGGGAAGCAATCAGCCCCAGCATTTT 2808 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGGGTTTTTAAGTCGCCCCAGCATTTT 2809 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTCCGGGACAGGGCTGATGGCTATGGCTACACCTTC 2810 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGGACGGGCGAGACCGGGGAGCTGTTTTTT 2811 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCTTACAGCCTATCTATGGCTACACCTTC 2812 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTCCTCGGGACTCATCTAGCACAGATACGCAGTATTTT 2813 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACCCTATGGCTAGCGGGAGTTGATGAGCAGTTCTTC 2814 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTTCCCGGACTAGCGGAGTTTCCTACGAGCAGTACTTC 2815 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGATATACCGGACAGGGCCACTCTGAACACCG GGGAGCTGTTTTTT 2816 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATAGCCCTCTGGGACTTCTACAATGAGCAGTTCTTC 2817 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCCCGACGGAGGGGGACGTTACGAGCAGTACTTC 2818 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTCGACAGCTACGAGCAGTACTTC 2819 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTTTTCTTTGCCACTGAAGCTTTCTTT 2820 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGGGACTAGCGGGAGAGCAGTTCTTC 2821 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGAATGGGGGGCCCGGGCTTTCTTT 2822 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAACTTGCCGGGCACTAGCGGGTTATCCACA GATACGCAGTATTTT 2823 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCGGCCTTCTCCGAGGGGTTGAAC ACTGAAGCTTTCTTT 2824 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGAGCTACTAGCACAGATACGCAGTATTTT 2825 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGAAGAAGGAGGGACGAGTATTCACCCCTCCACTTT 2826 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGATTTAGTTGGGACTAGCGGGAGGACCTACAA TGAGCAGTTCTTC 2827 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCTGGAGTCTCGCGGACTACGAGCAGTACTTC 2828 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCCGGGGGGAACACTGAAGCTTTCTTT 2829 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCTAGAGACCGGACTAGCGGGGATTACAATGAGCAGTTCTTC 2830 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCCCCGGCAAAGACCTACGAGCAGTACTTC 2831 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGCAGAGATTTTCGCGGCGAGCAGTACTTC 2832 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCTGGAGCCCAGAAAGGGATTCCTACGAGCAGTACTTC 2833 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGATAACGGGGGTGACACTGAAGCTTTCTTT 2834 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTCCTCCAGGGGAATGAGCAGTTCTTC 2835 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTCCCGCCGTGGGTGATAGGGAAAAACTGTTTTTT 2836 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGGAGGGTCGGTGAGCAGTTCTTC 2837 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCACCTCGGGGTCCCAGGTTGAGACCCAGTACTTC 2838 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCAAAGGGACAGCTACCTACGAGCAGTACTTC 2839 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGGGTATCTGGGGGCAGATACGCAGTATTTT 2840 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTCAGGTCCGCCGGGGAGCTGTTTTTT 2841 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGAAGGGTCTAGCGGGGGGGACGAGCAGTACTTC 2842 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGTCGCTTACTCCTACAATGAGCAGTTCTTC 2843 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTACCTCAAGAGACCCAGTACTTC 2844 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTTGGGACAGGGAGCTACGAGCAGTACTTC 2845 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTTGCACCTTACAATGAGCAGTTCTTC 2846 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGGGACGGTGGGGAACACTGAAGCTTTCTTT 2847 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGGGGCGGGAATCAGCCCCAGCATTTT 2848 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCGTGGGGTGGTTCTTC 2849 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTACTCGATCGGGGAGGATCAGCCCCAGCATTTT 2850 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTATGATAGGGGGAATTCACCCCTCCACTTT 2851 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCTGGAGTCTTAGCCCAGACAGTGAAGCTTTCTTT 2852 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGGTTCTGACAGGGTGACCTACGAGCAGTACTTC 2853 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCATGACAGGGGGTCAGTCACCCCTCCACTTT 2854 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGGGGGAAGGTGGGAGCTTTCTTT 2855 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTGTCTACGCGGGACAGGGTTACGAGCAGTACTTC 2856 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTTATCGTTTGGGGACTACGAGCAGTACTTC 2857 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAAGCTATGACAGGGGGCGCCGACTATGGC TACACCTTC 2858 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGGACAGGGCCAAGAGACCCAGTACTTC 2859 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAAGGACAGGGCGAGGACTGAAGCTTTCTTT 2860 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGTAGTATAGCGGGAGGGCCGCGGAATGAGCAGTTCTTC 2861 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGTGGGGACTGGGAGCTGATGGCTACACCTTC 2862 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCACTCTCGGGAGCTGGAAGATACGCAGTATTTT 2863 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGCGTCCGGTACAAATCAGCCCCAGCATTTT 2864 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGTTCCTTAGCCGACAGAACTAGGGGCTACACCTTC 2865 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAGGGACAACGGGCCTCCTACGAGCAGTACTTC 2866 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCTGAAAAGCTCCTACAATGAGCAGTTCTTC 2867 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCGGGCGACAGGGGCACACTGAAGCTTTCTTT 2868 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAAGGGGCGGAGCTGGCTCCTCTACGAGCAGTACTTC 2869 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCCGATGGGAGGGTTGAACACTGAAGCTTTCTTT 2870 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCAGTGCACTTAACAGGGGCCGCGGATACAATCAGCCCCAGCATTTT 2871 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCTGGAGTACGATTGACAATGAGCAGTTCTTC 2872 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTGGGGGGTCGGGAGCAGTTCTTC 2873 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCTCCCTCGCGAGCCGCAATATTCAAGAGACCCAGTACTTC 2874 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCTTGGCAAGTACTGAAGCTTTCTTT 2875 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTAGGGCCGGGACAGGGGGCCTACGAGCAGTACTTC 2876 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGCCAACTAGCGGGTAAAGAGACCCAGTACTTC 2877 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCACCCGACAGAGCAAAGCGGAGACCCAGTACTTC 2878 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCACCAGTGATTTAGGAGGGACCCAGCATTTT 2879 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCATCAGTGAGGGGTACGAGCAGTACTTC 2880 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCCAGGAAGGGTGGAAGTACGAGCAGTACTTC 2881 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCAGCTTATTTGATGAGGAAGAGACCCAGTACTTC 2882 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGTGCCAGCCAGGGACAGGGGGCTGGTTCACCCCTCCACTTT 2883 95-TL684-TIL-CD8+_CD103+ TIL CD8+_CD103+ TGCGCCAGCAGCCAAGCTTCGGGACTAGTCTTGAACACCGGG GAGCTGTTTTTT 2884 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGCCGTGGGGGGATACGCAGTATTTT 2885 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTATACGGGAAGGTACGAGCAGTACTTC 2886 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGTTCCGGGAGTAGGGTACGAGCAGTACTTC 2887 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTATTTTGGGGCTCGAACACTGAAGCTTTCTTT 2888 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGCCGCCAGTTCCCGATGGAATGAGCAGTTCTTC 2889 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTACAAGGAGATACGCAGTATTTT 2890 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTGGGGGTCCATGAGCAGTTCTTC 2891 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGCAAATCTACCCACAGATACGCAGTATTTT 2892 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGACGGAGGGGACACAGATACGCAGTATTTT 2893 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAATCTGTTTCCTACGAGCAGTACTTC 2894 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCATGCCTCCTCTGGAAACACCATATATTTT 2895 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGAATTAGGGGGGCAGCCCCAGCATTTT 2896 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCGCGGCCCACAGATACGCAGTATTTT 2897 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCACCAGCCCAGGGGGCCGGGGCTACACCTTC 2898 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGCCGGAGGATTCACCCCTCCACTTT 2899 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTAGGTTTACAGGCCAATTATGGCTACACCTTC 2900 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGCGATCAGGGCCTCAGGGCTACACCTTC 2901 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAGCACTAGCACATGAGCAGTTCTTC 2902 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCCCATAGGACAGGGTCAGATCAGCCCCAGCATTTT 2903 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTACAGGAGGGGTCGAGCAGTACTTC 2904 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGATCTAGCGGGAGGGCCTAGCACAGATACG CAGTATTTT 2905 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTCTACAGGGGATATGGCTACACCTTC 2906 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATTCGGGAGCTGAAGCTTTCTTT 2907 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAATGGGGGGCCTGAACACTGAAGCTTTCTTT 2908 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAGACCGGGAGCGGTCCCTACGAGCAGTACTTC 2909 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGGCTAACGTGGACAGATACCTACGAGCAGTACTTC 2910 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGAGACTGGCAGTCACTACAATGAGCAGTTCTTC 2911 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGCTAAAAGACTAGCGGTCTACAATGAGCAGTTCTTC 2912 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACCTGGGGCAAGAGACCCAGTACTTC 2913 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAAGAAGGACATCAAGAGACCCAGTACTTC 2914 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAACCAGGGGCTAGGCACTGAAGCTTTCTTT 2915 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGAGGAGACCCAGTACTTC 2916 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCACTTTATGGGGGGGGCAGTACAATGAGCAGTTCTTC 2917 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCTTTCCAGACTGAAGCTTTCTTT 2918 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAATATGGGCAGGGGGCGGCAACTAAT GAAAAACTGTTTTTT 2919 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAAGGACAGATGGGACATGAACACTGAAGCTTTCTTT 2920 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGGGACGGTGAGCAGTTCTTC 2921 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTATTCCGGGGGGAATGAGCAGTTCTTC 2922 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACAGGGGCTCCAATCAGCCCCAGCATTTT 2923 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCACAGGGGGGAACACTGAAGCTTTCTTT 2924 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGCGACAGGGGGGTACAATGAGCAGTTCTTC 2925 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTACTAGGGGGGACTAGCGGGAGGAATGA GCAGTTCTTC 2926 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACGGTTTTAGCGATGAGCAGTTCTTC 2927 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCTTAAACAGGGCAAATGAGCAGTTCTTC 2928 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCGCACCCGGGACGTACACTGAAGCTTTCTTT 2929 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTAGGGGGGCGTGGGGGGCGAACACTGAAGCTTTCTTT 2930 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTTTGGGCTAGCGGACGAGAGACCCAGTACTTC 2931 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATCACGGGGTCACCTACGAGCAGTACTTC 2932 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCACGTCGGGGACGGCTACACCTTC 2933 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGAGATCGCACAGAGAATTCACCCCTCCACTTT 2934 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCATCAGCGCTAGCGGGGGGGCAGGGTACAATGAGCAGTTCTTC 2935 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAGATCAAAGGGGTGACCTAAATGAGCAGTTCTTC 2936 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCTGAGGACGGTCGGAATGAAAAACTGTTTTTT 2937 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAGCGCGTACAGGACTCCAAGAGACCCAGTACTTC 2938 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTCAGAAGGGGTCGGTACGAGCAGTACTTC 2939 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAGGGATGGTCGGTCAATGAGCAGTTCTTC 2940 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGTCGGGGAGAGGGTCAGCCCCAGCATTTT 2941 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGACGGAGTCTCGGAGCAGTACTTC 2942 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCACATGACAAACTTCGACTCTGGAAACACCATATATTTT 2943 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGAAGAAGGGCTAGCGGGAGGAGGAGTAGATACGCAGTATTTT 2944 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTACCCCTGGGACAGGGGGATACGAGCAGTACTTC 2945 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGTAGGCGAAGAGACCCAGTACTTC 2946 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTCTGGACCTGACAGGGCCGAGCAGTACTTC 2947 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCAGGACTAGCGGGGCCCCCCAATGAGCAGTTCTTC 2948 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGACCCGCGGGGGGCCCTTACAATGAGCAGTTCTTC 2949 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCTAGGGGCCGAGCGGGTGGATGAGCAGTTCTTC 2950 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAAGATTGGTCTTGACAGGCCCTAATGAAAAA CTGTTTTTT 2951 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCTCGGACAGGGGAGTGGCCGGGGAGCTGTTTTTT 2952 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTACTCGACAGGGGGGGTTAGTACCGGGGAGC TGTTTTTT 2953 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGCAGATCGACAGGGGGGGGCACTGAAGCTTTCTTT 2954 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCGTCCTGGGACAGAAGGGTAAAGAGACCCAGTACTTC 2955 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCGAGGGGACTCAGCTCCTACAATGAGCAGTTCTTC 2956 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTGGACAGGAGTCACCGGGGAGCTGTTTTTT 2957 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGCGGGGACCTGAGCAGTTCTTC 2958 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGGATCAGGGGTTGAAGCTTTCTTT 2959 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCAGAGACTAGCAGACTACAATGAGCAGTTCTTC 2960 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATGGTACTAGCGGGGCCCCCTATGAGCAGTTCTTC 2961 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCACCCCTGCGGGGTTCCATAATGAAAAGCTGTTTTTT 2962 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTGAAGACAGGGCCGGCCAAGAGACCCAGTACTTC 2963 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTAGCCTCGGGCGGGGACTCCCAAGAGACCCAGTACTTC 2964 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATGCCGGGGGATACTATGGCTACACCTTC 2965 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCTGCTCCACGGGCAGGACGCAAGAGACCCAGTACTTC 2966 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTTGGCTACGAGCAGTACTTC 2967 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGCGTTGGGGACAGGGGACTAAAAGATACGCAGTATTTT 2968 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCCCAGGGAGTGGGGCTGGCTATGGCTACACCTTC 2969 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTTGGGGCTAGCGGGGCGCCCCTCTGAGCAGTTCTTC 2970 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCACCAGTGATGCCGCAACAGGGCGGTGGACCGGGGAGCTGTTTTTT 2971 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGTTTACATACTGGACTTACCTCCGAAGAGCAGTACTTC 2972 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCAGTGCTAGAGATACAGGACTAGAATACAATGAGCAGTTCTTC 2973 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGCGCCAGCAGCCAAGATTCGGGCTCTGGGGCCAACGTCCTGACTTTC 2974 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTAGGGGGGTTGGGCCGGGGGAGCAGTACTTC 2975 96-TL684-TIL-CD8+_CD103− TIL CD8+_CD103− TGTGCCAGCAGCTCCCGGGACAGGGCCTACGAGCAGTACTTC

TABLE 7 Specifically enriched genes in each 10x cluster padj. value. padj. value. padj. val ue. padj. value. Min. padj_Min. vs. V vs. IV vs. III vs. II log2FC log2FC FOS NA 1.66E−82 6.63E−42 5.90E−54 2.620190055 6.63E−42 TNFAIP3 NA 1.58E−77 1.79E−131 7.53E−21 2.265213659 7.53E−21 JUNB NA 1.68E−61 1.26E−30 4.72E−23 2.21557016 1.26E−30 ZFP36 NA 7.43E−53 2.03E−91 3.48E−29 2.918441617 3.48E−29 FOSB NA 2.20E−50 5.67E−17 2.48E−29 1.769295602 5.67E−17 TSC22D3 NA 8.38E−58  3.21E−132 1.27E−13 1.269885151 1.27E−13 KLF6 NA 7.95E−52 6.29E−50 1.66E−10 1.350296963 1.66E−10 IL7R NA 1.63E−47 1.84E−43 1.50E−82 2.602331444 1.84E−43 ANXA1 NA 7.69E−32 6.33E−21 8.62E−45 2.064086901 6.33E−21 RGCC NA 1.04E−45 1.06E−42 0.000401616 1.12087222 0.000401616 VIM NA 1.42E−24 4.76E−46 1.08E−29 2.631230111 1.42E−24 NFKBIA NA 2.17E−37 6.30E−24 2.97E−14 1.996504035 6.30E−24 GPR183 NA 1.81E−45 3.88E−52 3.00E−59 2.419298421 3.88E−52 FTH1 NA 7.43E−53 9.06E−85 1.81E−43 2.200051083 1.81E−43 ZFP36L2 NA 1.14E−22 1.90E−88 2.06E−21 1.884610725 2.06E−21 CD69 NA 1.51E−35 1.49E−56 2.18E−07 1.035505091 2.18E−07 IER2 NA 8.74E−21 0.002093792 7.85E−16 0.857291392 0.002093792 CXCR4 NA 2.28E−26  1.00E−120 5.50E−10 1.193942163 5.50E−10 REL NA 2.13E−21 3.31E−19 2.91E−09 1.369002869 2.91E−09 RORA NA 3.93E−17 5.06E−36 7.78E−13 1.87840742 3.93E−17 BTG2 NA 7.14E−22 0.000122431 6.57E−11 0.873222337 0.000122431 MYADM NA 7.69E−32 2.44E−27 4.11E−24 1.578811291 2.44E−27 ANKRD28 NA 1.16E−15 2.03E−19 7.49E−05 0.263208789 7.49E−05 MCL1 NA 1.50E−13 4.28E−26 2.30E−05 1.238637333 2.30E−05 PNRC1 NA 2.82E−10 1.70E−17 0.003629103 0.945575052 0.003629103 TUBA1A NA 3.17E−17 9.60E−08 4.14E−05 0.887158592 4.14E−05 CD44 NA 6.64E−11 3.37E−27 9.28E−09 1.629746169 6.64E−11 YPEL5 NA 1.92E−15 2.53E−22 1.76E−06 1.224033473 1.76E−06 PTGER4 NA 1.50E−13 2.38E−22 6.44E−05 1.204635078 6.44E−05 IFITM2 NA 1.09E−09 2.02E−05 2.34E−10 1.08193307 2.02E−05 SELK NA 3.40E−12 9.41E−13 0.005753149 0.727586968 0.005753149 S100A10 NA 2.56E−09 1.78E−16 2.25E−57 1.521791897 2.56E−09 PABPC1 NA 1.87E−47 1.78E−30 5.02E−27 0.884586386 1.78E−30 LMNA NA 1.12E−30 1.57E−40 1.29E−28 1.437010868 1.12E−30 LEPROTL1 NA 3.23E−10 1.68E−10 5.28E−06 1.061103392 5.28E−06 SAT1 NA 4.69E−07 1.98E−05 1.11E−15 0.095828191 1.98E−05 TOBI NA 3.73E−12 5.49E−21 1.45E−15 1.307119171 3.73E−12 FUS NA 1.06E−08 4.19E−06 1.89E−05 0.327001458 1.89E−05 ODF2L NA 6.35E−09 1.12E−08 2.90E−05 1.009673232 2.90E−05 IDS NA 3.82E−08 8.49E−11 1.21E−05 1.139317416 1.21E−05 DDX3X NA 8.31E−09 4.48E−07 0.000894747 0.474753873 0.000894747 SYTL3 NA 1.84E−06 5.40E−17 0.000253146 0.747116369 0.000253146 RP11-138A9 NA 5.39E−08 2.42E−06 9.96E−09 0.863328487 2.42E−06 FAM46C NA 5.06E−13 2.80E−34 3.51E−05 0.847977473 3.51E−05 CRIP1 NA 0.001624171 2.75E−10 0.009834552 0.94600557 0.009834552 ELF1 NA 0.000138316 2.05E−07 2.46E−05 0.598563385 2.46E−05 AHNAK NA 6.01E−08 6.41E−16 2.73E−11 1.007746639 6.01E−08 HIST1H4C NA 1.49E−06 7.72E−07 3.78E−08 0.930026463 7.72E−07 SDCBP NA 4.06E−08 1.41E−06 0.000141025 0.257116448 0.000141025 RPLP0 NA 0.008779115 0.003171103 1.72E−11 0.882868721 0.003171103 ARL4A NA 6.33E−13 3.74E−12 2.22E−08 0.84659306 3.74E−12 GPR65 NA 0.000107073 3.79E−11 0.001590716 0.701424179 0.001590716 H3F3B NA 4.72E−10 1.53E−16 2.42E−09 0.826890053 2.42E−09 IRF1 NA 1.23E−06 0.000197943 2.99E−11 0.665502687 0.000197943 RSL24D1 NA 6.36E−06 4.22E−06 0.003507975 0.570895999 0.003507975 EIF1 NA 1.02E−17 2.59E−48 0.002079569 0.347194993 0.002079569 JMJD1C NA 2.15E−06 0.000384594 0.001770106 0.69361394 0.000384594 VAMP2 NA 1.04E−05 4.00E−11 4.52E−06 0.845807406 4.52E−06 YWHAZ NA 0.001437739 2.04E−10 0.000145732 0.917970678 0.001437739 HOPX NA 0.000188042 8.77E−10 6.66E−27 0.91334475 0.000188042 FOSL2 NA 2.65E−10 7.72E−18 4.66E−08 0.749791815 4.66E−08 PIK3R1 NA 0.003094919 1.94E−12 4.00E−33 0.891986772 0.003094919 EML4 NA 0.002137792 1.53E−23 3.09E−10 0.842854321 0.002137792 BTG1 NA 7.69E−32 8.31E−60 5.26E−21 0.705782569 5.26E−21 CD55 NA 2.03E−08 1.07E−10 3.39E−13 0.814799096 2.03E−08 YBX3 NA 2.26E−09 1.19E−11 1.70E−13 0.806710572 1.19E−11 PDE4B NA 3.77E−06 9.92E−08 1.43E−05 0.806626131 3.77E−06 NFE2L2 NA 1.53E−06 5.49E−09 1.66E−06 0.753039458 1.66E−06 KLRC1 NA 1.26E−05 4.75E−12 5.03E−13 0.800761769 1.26E−05 RNF125 NA 2.54E−08 2.56E−15 0.002012095 0.634122788 0.002012095 RPS20 NA 6.54E−05 6.17E−15 8.67E−26 0.778950288 6.54E−05 MT-ND1 NA 0.007093368 3.72E−08 2.58E−05 0.775046717 0.007093368 LYAR NA 1.24E−05 7.84E−05 3.42E−31 0.674717264 7.84E−05 AC016831.7 NA 1.68E−08 3.03E−12 6.50E−06 0.769830524 1.68E−08 CCNL1 NA 0.004250662 0.000149318 0.000568016 0.663748163 0.000568016 RPS5 NA 0.003560905 6.62E−08 1.93E−12 0.751571098 0.003560905 SVIP NA 0.001454861 0.002421377 7.73E−05 0.638646302 0.002421377 RPS3A NA 4.69E−07 9.96E−18 3.51E−18 0.7292654 4.69E−07 RPL9 NA 3.11E−05 1.61E−14 1.60E−21 0.713109274 3.11E−05 PDCD4 NA 0.007227659 0.001269895 3.29E−08 0.696493816 0.007227659 FAM177A1 NA 0.001073118 0.000765204 1.63E−05 0.65792054 0.000765204 SBDS NA 3.73E−05 2.70E−07 2.70E−06 0.675014647 3.73E−05 SNRPG NA 5.61E−05 0.007121432 5.02E−06 0.288220813 5.02E−06 TUBA1B NA 0.005875971 0.00159609 0.005870285 0.56870654 0.005870285 VPS37B NA 2.90E−06 6.37E−17 0.002169337 0.466707881 0.002169337 CDC42SE2 NA 0.000318843 0.009794205 9.76E−07 0.426037826 0.009794205 RPL17 NA 0.000630659 0.001422255 1.12E−08 0.536793345 0.001422255 PARP8 NA 0.000786244 6.64E−06 6.32E−08 0.630954581 0.000786244 STAT4 NA 0.002183591 1.07E−12 6.36E−06 0.629984817 0.002183591 TAGLN2 NA 0.008068302 2.55E−07 4.11E−10 0.61816233 0.008068302 KDM6B NA 7.63E−09 9.02E−07 0.003503394 0.469156481 0.003503394 CSRNP1 NA 1.15E−08 3.86E−07 0.006290294 0.347032927 0.006290294 SERPINB9 NA 6.80E−05 0.001015828 0.005581702 0.265611197 0.005581702 MT-ND2 NA 6.27E−05 2.78E−22 1.77E−11 0.561392112 6.27E−05 MT-CO3 NA 0.001084082 7.15E−23 7.60E−05 0.470003688 7.60E−05 RPL14 NA 1.33E−05 9.62E−32 1.57E−29 0.541894813 1.33E−05 HNRNPUL1 NA 0.001475459 0.000513418 0.00070165 0.531020853 0.000513418 SKIL NA 0.002695606 2.83E−06 4.92E−07 0.535590273 0.002695606 EIF1AX NA 0.002892936 0.001131648 2.73E−05 0.522532099 0.002892936 PERP NA 0.00018877 7.16E−08 9.54E−16 0.498498324 0.00018877 DNAJB9 NA 3.01E−05 1.43E−07 0.000128016 0.489115584 0.000128016 TSC22D2 NA 1.16E−06 0.005292869 0.000113539 0.33233768 0.005292869 CCND3 NA 0.001133665 0.000122431 2.23E−05 0.488870983 0.001133665 FRMD4B NA 0.000908681 0.000501564 9.85E−09 0.445221505 0.000501564 RP11-138A9 NA 0.005651856 2.16E−07 8.35E−05 0.474233683 0.005651856 ATP2B1 NA 0.001485717 1.11E−06 3.36E−07 0.46857568 0.001485717 RTN4 NA 0.000805944 0.003453303 7.79E−05 0.375509687 7.79E−05 CD48 NA 0.009150862 0.000664974 7.86E−07 0.418732891 0.009150862 DSTN NA 0.002904998 0.000186724 1.06E−15 0.417567773 0.002904998 PSME4 NA 0.008174178 0.001185436 0.006823469 0.387246929 0.006823469 FXYD2 NA 1.92E−07 8.51E−08 3.74E−17 0.404561207 8.51E−08 RPS4X NA 0.000924117 1.20E−10 3.85E−28 0.381946846 0.000924117 MAP3K8 NA 0.000175794 6.51E−05 0.000127269 0.216967299 0.000127269 AUTS2 NA 0.005686021 4.23E−06 2.46E−09 0.371972221 0.005686021 RPL3 NA 0.001912992 1.99E−08 1.11E−24 0.361285551 0.001912992 MYBL1 NA 0.004982183 1.64E−06 6.07E−17 0.353506755 0.004982183 MIR29A NA 2.01E−06 1.49E−05 0.00070165 0.312081318 1.49E−05 RPS8 NA 0.005759353 7.12E−15 2.10E−26 0.321078519 0.005759353 PTMA NA 6.68E−12 3.30E−31 3.15E−06 0.173349586 3.15E−06 EEF1A1 NA 0.001809716 1.53E−25 2.68E−37 0.28930546 0.001809716 RPS12 NA 6.76E−08 2.50E−24 4.85E−60 0.258381871 6.76E−08 CLU NA 0.002664342 9.28E−05 3.65E−12 0.258347767 0.002664342 RPS14 NA 5.25E−05 1.75E−14 3.61E−47 0.239824473 5.25E−05 RPL32 NA 3.82E−05 3.88E−13 3.93E−40 0.214370857 3.82E−05 RPS25 NA 0.000648948 1.87E−11 2.53E−26 0.206461064 0.000648948 RPL39 NA 0.000565095 1.74E−16 2.80E−43 0.195344507 0.000565095 RPL34 NA 0.000820145 7.92E−18 2.10E−32 0.18751242 0.000820145 RPL10 NA 0.000501243 1.98E−14 7.53E−35 0.184865229 0.000501243 EMB NA 0.004669363 0.000271315 9.68E−05 0.165589987 0.004669363 RPLP2 NA 0.009961665 1.87E−26 3.62E−61 0.154274807 0.009961665 RPS29 NA 0.000799702 1.51E−28 3.69E−59 0.143164341 0.000799702 RAP1B NA 0.006692062 0.002021216 2.97E−12 0.066991093 0.006692062 CREM 0.000637878 2.23E−13 7.25E−17 NA 0.16411119 0.000637878 SAMSN1 4.40E−06 3.48E−07 3.53E−13 NA 0.249849391 4.40E−06 SRGN 1.31E−06 4.57E−13 6.71E−21 NA 0.037430009 1.31E−06 SRSF5 1.90E−07 1.46E−08 3.95E−12 NA 0.035995217 1.90E−07 BIRC3 0.000244594 3.20E−09 0.002259756 NA 0.410890215 0.000244594 PHLDA1 9.04E−14 1.41E−29 3.04E−21 NA 1.460838105 9.04E−14 STAT3 2.48E−14 2.67E−12 4.05E−09 NA 1.210310952 2.48E−14 ETS1 3.31E−19 0.001454482 2.00E−15 NA 1.228327744 3.31E−19 ETV1 3.83E−21 9.49E−05 0.001411419 NA 0.477436057 0.001411419 NEAT1 2.24E−25 7.06E−13 2.70E−05 NA 1.108735317 2.70E−05 KRT86 5.53E−60 1.68E−26 3.58E−28 NA 1.991594638 3.58E−28 AKAP5 2.08E−34 8.64E−11 0.000495119 NA 0.737927495 0.000495119 HLA-DQA1 4.38E−33 1.38E−06 1.21E−07 NA 0.973184844 1.21E−07 CXCL13  1.21E−146 2.32E−69 7.62E−40 NA 3.126576592 7.62E−40 CHN1 7.22E−65 3.28E−21 2.81E−27 NA 1.8586028 3.28E−21 TNIP3 2.53E−28 1.10E−08 3.42E−05 NA 0.96911344 3.42E−05 TIGIT 4.29E−61 1.12E−22 8.59E−14 NA 1.790082066 8.59E−14 LYST 1.57E−24 1.95E−05 1.25E−08 NA 1.197928623 1.95E−05 CTSW 5.45E−23 0.000690254 3.98E−07 NA 1.430011984 3.98E−07 HAVCR2 6.29E−78 2.02E−26 4.86E−12 NA 1.56926631 4.86E−12 AMICA1 2.10E−32 2.93E−06 1.15E−12 NA 1.557097802 2.93E−06 ALOX5AP 1.73E−26 0.003772 8.85E−11 NA 1.181535785 0.003772 AC002331.1 2.87E−63 3.61E−24 6.17E−11 NA 1.763215521 6.17E−11 AC092580.4 5.63E−85 1.60E−19 1.44E−29 NA 1.490695634 1.60E−19 SIRPG 4.37E−37 0.000329935 2.45E−05 NA 0.960381084 2.45E−05 HLA-DRA 2.43E−50 2.04E−11 1.27E−10 NA 1.700525516 1.27E−10 CD74 7.78E−36 2.60E−05 2.48E−06 NA 1.493444518 2.48E−06 SRGAP3 1.82E−43 6.23E−11 4.12E−08 NA 1.377296108 4.12E−08 HLA-DPA1 1.73E−29 0.003946769 5.07E−05 NA 1.105636301 0.003946769 AC069363.1 4.52E−43 3.02E−08 1.52E−05 NA 1.0532031 1.52E−05 RBPJ 5.99E−97 2.06E−33 6.86E−37 NA 3.430066413 6.86E−37 NKG7 7.63E−23 0.00490394 0.003416563 NA 0.615222066 0.00490394 HLA-DPB1 2.31E−33 0.00399397 2.47E−07 NA 1.17285258 0.00399397 ENTPD1  7.75E−118 1.44E−25 5.10E−18 NA 2.237692196 5.10E−18 HLA-DRB1 1.08E−55 1.10E−09 9.61E−10 NA 1.867004057 9.61E−10 GZMA 1.09E−42 0.003619451 2.76E−10 NA 0.717131097 0.003619451 RGS1 5.90E−76 1.22E−46  2.72E−103 NA 2.731407573 5.90E−76 RP11-347P5. 0.000420226 0.001942401 3.51E−07 NA 0.2373155 0.000420226 CLEC2B 0.000244272 3.65E−05 1.16E−11 NA 0.557073889 0.000244272 RNF19A 6.01E−20 1.56E−16 1.25E−26 NA 1.534939563 6.01E−20 KRT81 5.98E−13 8.34E−05 0.003052277 NA 0.376158507 0.003052277 RP11-279F6. 9.44E−16 0.004695777 0.002043077 NA 0.353215743 0.004695777 TNS3 1.13E−14 1.48E−09 0.003798297 NA 0.347046461 0.003798297 MAST4 1.69E−14 0.000994731 0.000913076 NA 0.599970917 0.000913076 LAYN 2.17E−35 1.42E−17 9.39E−10 NA 0.858923999 9.39E−10 TNFRSF18 1.34E−22 2.61E−12 2.91E−05 NA 0.651260673 2.91E−05 VCAM1 3.43E−31 3.78E−15 1.36E−07 NA 0.892616431 1.36E−07 AHI1 6.00E−25 1.59E−09 4.53E−05 NA 0.809032683 4.53E−05 ACP5 5.05E−18 3.58E−05 0.00381038 NA 0.699716551 0.00381038 TNFRSF9 8.16E−53 1.44E−25 2.38E−09 NA 1.010907938 2.38E−09 RAB27A 9.38E−19 0.000457807 0.009467496 NA 0.863443141 0.009467496 SLA 7.06E−20 0.000223607 0.007230644 NA 1.008695747 0.007230644 ITGAE 1.44E−23 8.44E−09 1.16E−06 NA 1.404487179 1.16E−06 CRTAM 1.72E−34 1.03E−12 5.22E−08 NA 1.273678799 5.22E−08 CTLA4 2.95E−108 5.96E−57 8.96E−54 NA 3.634958175 8.96E−54 CCL3 3.08E−42 6.42E−22 1.83E−14 NA 2.186450539 1.83E−14 IFNG 3.98E−40 1.24E−19 6.99E−09 NA 1.926260214 6.99E−09 CYSLTR1 0.003483664 0.00846443 0.000542256 NA 0.26981673 0.003483664 HLA-A 7.70E−07 0.000575743 2.83E−16 NA 0.222076438 0.000575743 RGS13 4.29E−09 1.12E−07 4.12E−08 NA 0.345333169 4.12E−08 IL26 2.58E−06 3.16E−05 1.41E−05 NA 0.335981266 1.41E−05 IL17A 4.68E−09 4.38E−06 2.96E−07 NA 0.387444572 2.96E−07 MYO1E 1.12E−08 0.000787688 3.80E−09 NA 0.356887185 0.000787688 TNFSF4 5.04E−09 1.30E−05 0.000906747 NA 0.310849396 0.000906747 AFAP1L2 1.97E−13 4.89E−06 3.55E−05 NA 0.40411992 3.55E−05 AGFG1 1.32E−06 0.000172958 0.000146563 NA 0.425946297 0.000146563 CSGALNACT1 2.21E−09 0.001466406 0.000268602 NA 0.385809284 0.000268602 CBLB 3.42E−05 0.005678513 0.003085899 NA 0.581796519 3.42E−05 PDCD1 2.72E−06 0.003085605 0.008364075 NA 0.526289264 0.008364075 CLECL1 5.40E−08 2.52E−05 1.39E−09 NA 0.762149336 2.52E−05 ARID5B 4.13E−07 3.93E−06 1.48E−06 NA 0.794915971 4.13E−07 ARL3 1.65E−13 0.002114441 0.005412229 NA 0.509782342 0.005412229 SNX9 2.86E−11 1.39E−08 4.92E−09 NA 0.768488877 4.92E−09 NR3C1 1.50E−06 5.68E−06 3.96E−07 NA 0.874460583 1.50E−06 PRDM1 5.23E−12 0.00266954 1.20E−06 NA 0.88275225 5.23E−12 ICOS 3.02E−06 1.22E−09 3.50E−11 NA 0.88604441 3.02E−06 MIR155HG 1.22E−14 3.12E−05 1.48E−06 NA 0.693045083 1.48E−06 CD7 1.79E−10 0.003289914 2.94E−06 NA 0.916916051 1.79E−10 PTPN22 2.33E−09 0.001275942 3.66E−10 NA 0.968242771 2.33E−09 CALR 5.60E−08 0.009999931 5.51E−05 NA 0.982686939 0.009999931 ID2 6.42E−16 0.000170191 3.77E−05 NA 0.875841563 3.77E−05 PRF1 1.75E−07 1.50E−05 0.002263138 NA 1.048524967 0.002263138 TOX 3.13E−20 6.37E−05 9.42E−11 NA 0.769197704 6.37E−05 GZMB 1.34E−06 3.51E−10 5.32E−08 NA 1.221935285 1.34E−06 ZEB2 1.86E−10 9.42E−11 9.00E−15 NA 1.249618159 1.86E−10 PAG1 7.77E−15 1.26E−06 2.52E−05 NA 1.040188511 2.52E−05 KLRD1 1.12E−14 0.000204255 5.79E−06 NA 1.221275836 5.79E−06 CLEC2D 1.84E−16 1.74E−07 1.74E−12 NA 1.425987745 1.84E−16 HLA-DRB5 1.53E−27 1.74E−06 2.66E−15 NA 1.098554982 1.74E−06 ITM2A 2.55E−25 1.24E−09 6.71E−10 NA 1.681694597 6.71E−10 DUSP4 7.60E−63 2.03E−45 1.70E−46 NA 3.575838252 1.70E−46 PPP1R15A 2.41E−11 3.42E−18 NA 2.24E−11 0.464034832 2.41E−11 JUN 1.87E−26 3.15E−65 NA 9.37E−16 1.180205182 1.87E−26 DNAJA1 1.69E−36 1.94E−44 NA 8.68E−31 1.876239108 1.69E−36 GADD45B 0.0001058 1.82E−07 NA 2.73E−21 0.43259821 0.0001058 HSP90AB1 2.58E−56 1.36E−40 NA 4.55E−37 1.994368885 2.58E−56 NEU1 9.13E−08 1.02E−21 NA 2.18E−10 0.769179396 9.13E−08 HSPA6  8.48E−145 5.35E−76 NA 8.16E−76 3.408403253 5.35E−76 AC006129.2 7.48E−27 6.94E−07 NA 0.000474985 0.931576889 0.000474985 HSP90AA1  4.56E−228  9.78E−136 NA  7.31E−122 2.867834259 9.78E−136 HSPE1  1.66E−152 2.75E−77 NA 5.06E−70 3.80539206 2.75E−77 HSPB1  1.44E−204  8.65E−101 NA 8.71E−71 4.39192139  8.65E−101 HSPA1B 0  1.59E−166 NA  3.66E−167 5.75800638  1.59E−166 HSPA1A 0  5.20E−156 NA  3.71E−173 5.638622319  5.20E−156 MRPL18 1.29E−05 7.33E−05 NA 7.65E−06 0.464014474 1.29E−05 HIST2H2AA3 0.00327089 3.93E−09 NA 4.40E−06 0.475956969 0.00327089 C17orf67 6.60E−10 0.00308263 NA 0.001284567 0.380442359 0.00308263 GPR113 8.30E−17 3.89E−08 NA 1.22E−07 0.499732558 3.89E−08 TRA2B 1.17E−05 6.18E−06 NA 1.54E−07 0.606306382 1.17E−05 TCP1 6.70E−08 0.002311184 NA 0.000213795 0.530155811 0.002311184 HSD17B7 2.72E−13 0.000222937 NA 0.00019165 0.461103562 0.000222937 NUDT4 1.26E−05 1.06E−06 NA 8.03E−06 0.769380579 1.26E−05 NR4A1 2.03E−10 2.32E−16 NA 5.32E−08 0.865822254 2.03E−10 DNAJA4 1.11E−25 4.91E−15 NA 9.33E−12 0.803134197 4.91E−15 MB21D1 1.14E−18 1.31E−05 NA 5.04E−12 0.650130282 1.31E−05 SERPIN H1 1.20E−28 6.61E−15 NA 5.04E−12 0.829860665 6.61E−15 DONSON 1.03E−14 0.002853788 NA 2.71E−15 0.736069594 0.002853788 ZFAND2A 4.16E−20 6.69E−16 NA 4.41E−12 1.066658182 4.16E−20 TSPYL2 1.28E−08 3.62E−16 NA 1.85E−05 1.080709987 1.28E−08 UGP2 1.71E−15 5.63E−06 NA 0.000844439 0.884934197 5.63E−06 MXD1 5.46E−20 1.92E−15 NA 2.65E−09 1.069026257 2.65E−09 FTL 1.02E−28 1.66E−13 NA 6.54E−22 1.169331909 1.02E−28 UBB 1.63E−29 1.32E−20 NA 1.47E−10 1.24307025 1.63E−29 BAG3 1.73E−45 2.85E−32 NA 4.82E−18 1.224624597 4.82E−18 CHORDC1 2.08E−27 7.44E−17 NA 1.92E−09 1.268567875 1.92E−09 UBC 8.93E−39 5.84E−34 NA 1.41E−05 0.902441099 1.41E−05 DNAJB4 5.52E−56 1.09E−42 NA 6.21E−28 2.038178703 1.09E−42 CACYBP 1.19E−52 2.35E−34 NA 1.53E−29 2.116008605 2.35E−34 HSPH1 7.63E−84 3.15E−65 NA 8.68E−50 2.988971735 3.15E−65 HSPA8 3.87E−75 2.06E−53 NA 1.54E−52 3.185712724 2.06E−53 HSPD1 5.83E−89 6.15E−53 NA 1.72E−65 2.942737466 6.15E−53 RGS2 1.16E−83 1.09E−61 NA 0.003360863 1.196385902 0.003360863 DNAJB1  1.15E−221  1.35E−148 NA 2.53E−160 4.824072522  1.35E−148 CD52 2.69E−08 NA 3.20E−06 5.80E−05 0.484125919 2.69E−08 ATP5E 1.03E−12 NA 0.000456224 0.00057577 0.433144665 0.000456224 IL32 6.33E−19 NA 9.55E−06 4.69E−05 0.480236359 9.55E−06

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What is claimed is:
 1. A method of treating cancer and/or eliciting an anti-tumor response in a subject comprising administering to the subject an effective amount of a population of T-cells that exhibit higher than or lower than baseline expression of one or more genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7, or that express a T-cell receptor comprising at least one of the amino acid sequences set forth in Table 6, thereby treating cancer and/or eliciting an anti-tumor response in the subject.
 2. A method of treating cancer and/or eliciting an anti-tumor response in a subject comprising administering to the subject an effective amount of an agent that induces higher than or lower than baseline expression of one or more genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 in T-cells, or a T-cell receptor comprising at least one of the amino acid sequences set forth in Table 6, thereby treating cancer and/or eliciting an anti-tumor response in the subject.
 3. A method of treating cancer and/or eliciting an anti-tumor response in a subject or sample comprising administering an effective amount of one or more an agent that induces or inhibits in T-cells activity of one or more proteins encoded by genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 to the subject, thereby treating cancer and/or eliciting an anti-tumor response in the subject.
 4. The method of any one of claims 1 to 2, wherein the T-cells are tissue-resident memory cells (TRM) or CD8+ T-cells.
 5. The method of claim 4, wherein the T-cells are autologous to the subject being treated.
 6. The method of any one of claims 1 to 5, wherein the one or more gene or all of the genes from the group of 4-1BB, PD-1, CD103 or TIM3.
 7. The method of any one of claims 1 to 6, wherein baseline expression is normalized mean gene expression.
 8. The method of claim 7, wherein higher than baseline expression is at least about a 2-fold increase in expression relative to baseline expression and/or lower than baseline expression is at least about a 2-fold decrease in expression relative to baseline expression.
 9. The method of any one of claims 2 to 8, wherein the active agent is an antibody, a small molecule, a protein, a peptide, a ligand mimetic or a nucleic acid.
 10. The method of any one of claims 1 to 9, further comprising administering to the subject an effective amount of a cytoreductive therapy.
 11. The method of claim 10, wherein the cytoreductive therapy is one or more of chemotherapy, immunotherapy, or radiation therapy.
 12. A modified T-cell modified to exhibit higher than or lower than baseline expression of one or more genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7, or to express a T-cell receptor comprising at least one of the amino acid sequences set forth in Table
 6. 13. The modified T-cell of claim 12, wherein the one or more gene is selected from 4-1BB, PD-1, CD103 or TIM3.
 14. The modified T-cell of claim 12 or 13, wherein the T-cell is a tissue-resident memory cell (TRM) or a CD8+ T-cell.
 15. The modified T-cell of any one of claims 12 to 14, wherein the T-cell the T-cells are autologous to the subject being treated.
 16. The modified T-cell of any one of claims 12 to 15, wherein baseline expression is normalized mean gene expression.
 17. The modified T-cell of claim 16, wherein higher than baseline expression is at least about a 2-fold increase in expression relative to baseline expression and/or lower than baseline expression is at least about a 2-fold decrease in expression relative to baseline expression.
 18. The modified T-cell of any one of claims 12 to 17, wherein the modified T-cell is genetically modified, optionally using one or more of gene editing, recombinant methods and/or a CRISPR/Cas system.
 19. The modified T-cell of any one of claims 12 to 18, further modified to express a protein that binds to a cytokine, chemokine, lymphokine, or a receptor each thereof.
 20. The modified T-cell of claim 19, wherein the protein comprises an antibody or an antigen binding fragment thereof.
 21. The modified T-cell of claim 20, wherein the antibody is an IgG, IgA, IgM, IgE or IgD, or a subclass thereof.
 22. The modified T-cell of claim 21, wherein the antibody is an IgG selected from the group of IgG1, IgG2, IgG3 or IgG4.
 23. The modified T-cell of any one of claims 20 to 22, wherein the antigen binding fragment is selected from the group of a Fab, Fab′, F(ab′)2, Fv, Fd, single-chain Fvs (scFv), disulfide-linked Fvs (sdFv) or V_(L) or V_(H).
 24. The modified T-cell of any one of claims 12 to 23, wherein the modified T-cell comprises a chimeric antigen receptor (CAR).
 25. The modified T-cell of claim 24, wherein the chimeric antigen receptor (CAR) comprises: (a) an antigen binding domain; (b) a hinge domain; (c) a transmembrane domain; (d) and an intracellular domain.
 26. The modified T-cell of claim 25, wherein the CAR further comprises one or more costimulatory signaling regions.
 27. The modified T-cell of claim 26, wherein the antigen binding domain comprises an anti-CD19 antigen binding domain, the transmembrane domain comprises a CD28, CD28H (TMIGD2), AMICA1 or a CD8 α transmembrane domain, the one or more costimulatory regions selected from a CD28 costimulatory signaling region, a 4-1BB costimulatory signaling region, an ICOS costimulatory signaling region, an AMICA1 costimulatory signaling region, a CD28H (TMIGD2) costimulatory signaling region, and an OX40 costimulatory region or a CD3 zeta signaling domain.
 28. The modified T-cell of claim 27, wherein the anti-CD19 binding domain comprises a single-chain variable fragment (scFv) that specifically recognizes a humanized anti-CD19 binding domain.
 29. The modified T-cell of claim 27 or 28, wherein the anti-CD19 binding domain scFv of the CAR comprises a heavy chain variable region and a light chain variable region.
 30. The modified T-cell of claim 29, wherein the anti-CD19 binding domain of the CAR further comprises a linker polypeptide located between the anti-CD19 binding domain scFv heavy chain variable region and the anti-CD19 binding domain scFv light chain variable region.
 31. The modified T-cell of claim 30, wherein the linker polypeptide of the CAR comprises a polypeptide of the sequence (GGGGS)n wherein n is an integer from 1 to
 6. 32. The modified T-cell of any one of claims 24 to 31, wherein the CAR further comprises a detectable marker attached to the CAR.
 33. The modified T-cell of any one of claims 24 to 32, wherein the CAR further comprises a purification marker attached to the CAR.
 34. The modified T-cell of any one of claims 24 to 33, wherein the modified T-cell comprises a polynucleotide encoding the CAR, and optionally, wherein the polynucleotide encodes and anti-CD19 binding domain.
 35. The modified T-cell of claim 34, wherein the polynucleotide further comprises a promoter operatively linked to the polynucleotide to express the polynucleotide in the modified T-cell.
 36. The modified T-cell of claim 34, wherein the polynucleotide further comprises a 2A self-cleaving peptide (T2A) encoding polynucleotide sequence located upstream of a polynucleotide encoding the anti-CD19 binding domain.
 37. The modified T-cell of any one of claims 34 to 36, wherein the polynucleotide further comprises a polynucleotide encoding a signal peptide located upstream of a polynucleotide encoding the anti-CD19 binding domain.
 38. The modified T-cell of any one of claims 33 to 37, wherein the polynucleotide further comprises a vector.
 39. The modified T-cell of claim 38, wherein the vector is a plasmid.
 40. The modified T-cell of claim 38, wherein the vector is a viral vector selected from the group of a retroviral vector, a lentiviral vector, an adenoviral vector, and an adeno-associated viral vector.
 41. A composition comprising a population of modified T-cells according to any one of claims 12 to
 40. 42. A method of treating cancer in a subject and/or eliciting an anti-tumor response comprising administering to the subject or contacting the tumor with an effective amount of the modified T-cells according to any one of claims 12 to 40 and/or the composition according to claim 40, thereby treating cancer and/or eliciting an anti-tumor response in the subject.
 43. A method of diagnosing cancer, comprising contacting a sample isolated from the subject with an agent that detects the presence of one or more genes set forth in Table 1, Table 2, Table 3, Table 4, Table Sand/or Table 7 in the sample isolated from the subject, wherein the presence of the one or more genes at higher or lower than baseline expression levels is diagnostic of cancer.
 44. A method of diagnosing cancer, comprising contacting tissue-resident memory cells (TRMs) or a cancer sample isolated from the subject with an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAG3, an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds CD28H (TMIGD2), and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of CD8⁺PD1⁺, CD8⁺TIM3⁺, CD8⁺LAG3⁺, CD8⁺AMICA1⁺, CD8⁺CD28H⁺, CD8⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺, CD8⁺PD1⁺LAG3⁺, CD8⁺PD1⁺AMICA1⁺, CD8⁺PD1⁺CD28H⁺, CD8⁺PD1⁺CTLA4⁺, CD8⁺TIM3⁺LAG3⁺, CD8⁺TIM3⁺AMICA1⁺, CD8⁺TIM3⁺CD28H⁺, CD8⁺TIM3⁺CTLA4⁺′CD8⁺LAG3⁺CTLA4+, CD8⁺LAG3⁺AMICA1⁺, CD8⁺LAG3⁺CD28H⁺, CD8⁺PD1⁺TIM3⁺LAG3⁺, CD8⁺LAG3⁺PD1AMICA1⁺, CD8⁺LAG3⁺PD1⁺CD28H⁺, CD8^(+PD)1⁺LAG3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺AMICA1⁺′, CD8⁺PD1⁺TIM3⁺CTLA4⁺CD28H⁺′ or CD8⁺PD1⁺TIM3⁺CTLA4⁺AMICA⁺CD28H⁺′ TRMs, wherein a high frequency of one or more of these TRMs is diagnostic of cancer.
 45. A method of diagnosing cancer in a subject comprising contacting tissue-resident memory cells (TRMs) isolated from the subject or cancer sample isolated from the subject, with an antibody or agent that recognizes and binds one or more proteins encoded by a gene set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 and, optionally, an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAG3, an antibody or agent that recognizes and binds CD28H (TMIGD2), an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds KLF3, an antibody or agent that recognizes and binds S1PR5, an antibody or agent that recognizes and binds S1PR1, an antibody or agent that recognizes and binds KLF2 and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of TRMs expressing these proteins, wherein a high frequency of TRMs expressing these proteins is diagnostic of cancer.
 46. A method of determining the density of tissue-resident memory cells (TRMs) in a cancer, tumor, or sample thereof comprising measuring expression of one or more gene selected from the group of 4-1BB, PD-1, CD103 or TIM3 or genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 in the cancer, tumor, or sample thereof, wherein higher or lower than baseline expression indicates higher density of TRMs in the cancer, tumor, or sample thereof.
 47. A method of determining prognosis of a subject having cancer comprising measuring the density of tissue-resident memory cells (TRM) in a sample isolated from the subject, wherein a high density of TRM indicates a more positive prognosis, e.g., an increased probability and/or duration of survival.
 48. A method of determining prognosis of a subject having cancer comprising contacting tissue-resident memory cells (TRMs) isolated from the subject with an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAG3, an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds CD28H (TMIGD2), and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of CD8⁺PD1⁺, CD8⁺TIM3⁺, CD8⁺LAG3⁺, CD8⁺AMICA1⁺, CD8⁺CD28H⁺, CD8⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺, CD8⁺PD1⁺LAG3⁺, CD8⁺PD1⁺AMICA1⁺, CD8⁺PD1⁺CD28H⁺, CD8⁺PD1⁺CTLA4⁺, CD8⁺TIM3⁺LAG3⁺, CD8⁺TIM3⁺AMICA1⁺, CD8⁺TIM3⁺CD28H⁺, CD8⁺TIM3⁺CTLA4⁺, CD8⁺LAG3⁺CTLA4⁺, CD8⁺LAG3⁺AMICA1⁺, CD8⁺LAG3⁺CD28H⁺, CD8⁺PD1⁺TIM3⁺LAG3⁺, CD8⁺LAG3⁺PD1⁺AMICA1⁺, CD8⁺LAG3⁺PD1⁺CD28H⁺, CD8⁺PD1⁺LAG3⁺CTLA4⁺, CD8^(+PD)1⁺TIM3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺AMICA1⁺′, CD8⁺PD1⁺TIM3⁺CTLA4⁺CD28H⁺′ or CD8^(+PD)1⁺TIM3⁺CTLA4⁺AMICA⁺CD28H⁺′ TRMs, wherein a high frequency of one or more of these TRMs indicates a more positive prognosis, e.g., an increased probability and/or duration of survival.
 49. A method of determining prognosis of a subject having cancer comprising contacting tissue-resident memory cells (TRMs) isolated from the subject with an antibody or agent that recognizes and binds one or more proteins encoded by a gene set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 and, optionally, an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAG3, an antibody or agent that recognizes and binds CD28H (TMIGD2), an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds KLF3, an antibody or agent that recognizes and binds S1PR5, an antibody or agent that recognizes and binds S1PR1, an antibody or agent that recognizes and binds KLF2 and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of TRMs expressing these proteins, wherein a high frequency of TRMs expressing these proteins indicates a more positive prognosis, e.g., an increased probability and/or duration of survival.
 50. A method of determining prognosis of a subject having cancer comprising contacting tissue-resident memory cells (TRMs) isolated from the subject with an antibody or agent that recognizes and binds CD103 to determine the frequency of CD103+ TRMs or an antibody that recognizes and binds a protein encoded by a gene set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 to determine the frequency of TRMs expressing the protein, wherein a high or low frequency of TRMs expressing the protein indicates a more positive prognosis, e.g., an increased probability and/or duration of survival.
 51. A method of determining the responsiveness of a subject having cancer to immunotherapy comprising contacting tissue-resident memory cells (TRMs) isolated from the subject with an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAG3, an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds CD28H (TMIGD2), and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of CD8⁺PD1⁺, CD8⁺TIM3⁺, CD8⁺LAG3⁺, CD8⁺AMICA1⁺, CD8⁺CD28H⁺, CD8⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺, CD8⁺PD1⁺LAG3⁺, CD8⁺PD1⁺AMICA1⁺, CD8⁺PD1⁺CD28H⁺, CD8⁺PD1⁺CTLA4⁺, CD8⁺TIM3⁺LAG3⁺, CD8⁺TIM3⁺AMICA1⁺, CD8⁺TIM3⁺CD28H⁺, CD8⁺TIM3⁺CTLA4⁺, CD8⁺LAG3⁺CTLA4⁺, CD8⁺LAG3⁺AMICA1⁺, CD8⁺LAG3⁺CD28H⁺, CD8⁺PD1⁺TIM3⁺LAG3⁺, CD8⁺LAG3⁺PD1⁺AMICA1⁺, CD8⁺LAG3⁺PD1⁺CD28H⁺, CD8⁺PD1⁺LAG3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺, CD8⁺PD1⁺TIM3⁺CTLA4⁺AMICA1⁺′, CD8⁺PD1⁺TIM3⁺CTLA4⁺CD28H⁺′ or CD8⁺PD1⁺TIM3⁺CTLA4⁺AMICA⁺CD28H⁺, TRMs, wherein a high frequency of one or more of these TRMs indicates responsiveness to immunotherapy.
 52. A method of determining the responsiveness of a subject having cancer to immunotherapy comprising contacting tissue-resident memory cells (TRMs) isolated from the subject with an antibody or agent that recognizes and binds one or more proteins encoded by a gene set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 and, optionally, an antibody or agent that recognizes and binds CD8, an antibody or agent that recognizes and binds PD-1, an antibody or agent that recognizes and binds TIM3, an antibody or agent that recognizes and binds LAG3, an antibody or agent that recognizes and binds CD28H (TMIGD2), an antibody or agent that recognizes and binds AMICA1, an antibody or agent that recognizes and binds KLF3, an antibody or agent that recognizes and binds S1PR5, an antibody or agent that recognizes and binds S1PR1, an antibody or agent that recognizes and binds KLF2 and an antibody or agent that recognizes and binds CTLA4 to determine the frequency of TRMs expressing these proteins, wherein a high frequency of TRMs expressing these proteins indicates responsiveness to immunotherapy.
 53. The method of any of claims 43 to 52, wherein the TRMs are CD19-CD20-CD14-CD56-CD4-CD45⁺CD3⁺CD8+ T-cells.
 54. A method of determining prognosis of a subject having cancer comprising measuring the density of CD103 or proteins encoded by one or more gene set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 in a sample isolated from the subject, wherein a high or low density of proteins indicates a more positive prognosis, e.g., an increased probability and/or duration of survival.
 55. A method of identifying a subject that will or is likely to respond to a cancer therapy, comprising contacting a sample isolated from the subject with an agent that detects the presence of one or more genes set forth in Table 1, Table 2, Table 3, Table 4, Table 5 and/or Table 7 in the sample, wherein the presence of the one or more genes at higher or lower than baseline expression levels indicates that the subject is likely to respond to cancer therapy.
 56. The method of any one of claim 43, 46 or 55, wherein baseline expression is normalized mean gene expression.
 57. The method of claim 56, wherein higher than baseline expression is at least about a 2-fold increase in expression relative to baseline expression and/or lower than baseline expression is at least about a 2-fold decrease in expression relative to baseline expression.
 58. The method of any one of claims 43 to 57, further comprising administering a cancer therapy to the subject.
 59. The method of claim 58, wherein the cancer therapy is chemotherapy, immunotherapy, radiation therapy, and/or administering to the subject or contacting the tumor with an effective amount of the modified T-cells according to any one of claims 12 to 40 and/or the composition according to claim
 40. 60. The method of any one of claims 43 to 59, wherein the cancer, tumor, or sample is contacted with an agent, optionally including a detectable label or tag.
 61. The method of claim 60, wherein the detectable label or tag comprises a radioisotope, a metal, horseradish peroxidase, alkaline phosphatase, avidin or biotin.
 62. The method of claim 60 or 61, wherein the agent comprises a polypeptide that binds to an expression product encoded by the gene, or a polynucleotide that hybridizes to a nucleic acid sequence encoding all or a portion of the gene.
 63. The method of claim 62, wherein the polypeptide comprises an antibody, an antigen binding fragment thereof, or a receptor that binds to the gene.
 64. The method of claim 63, wherein the antibody is an IgG, IgA, IgM, IgE or IgD, or a subclass thereof.
 65. The method of claim 64, wherein the IgG is an IgG1, IgG2, IgG3 or IgG4.
 66. The method of any one of claims 63 to 65 wherein the antigen binding fragment is a Fab, Fab′, F(ab′)2, Fv, Fd, single-chain Fvs (scFv), disulfide-linked Fvs (sdFv) or VL or VH.
 67. The method of any one of claims 43 to 66, wherein the agent is contacted with the cancer, tumor, or sample in conditions under which it can bind to the gene it targets.
 68. The method of any one of claims 43 to 67, wherein the method comprises detection by immunohistochemistry (IHC), in-situ hybridization (ISH), ELISA, immunoprecipitation, immunofluorescence, chemiluminescence, radioactivity, X-ray, nucleic acid hybridization, protein-protein interaction, immunoprecipitation, flow cytometry, Western blotting, polymerase chain reaction, DNA transcription, Northern blotting and/or Southern blotting.
 69. The method of any one of claims 43 to 68, wherein the sample comprises cells, tissue, an organ biopsy, an epithelial tissue, a lung, respiratory or airway tissue or organ, a circulatory tissue or organ, a skin tissue, bone tissue, muscle tissue, head, neck, brain, skin, bone and/or blood sample.
 70. The method of any one of claims 1 to 11 or claims 41 to 69, wherein the cancer or tumor is an epithelial, a head, neck, lung, lung, prostate, colon, pancreas, esophagus, liver, skin, kidney, adrenal gland, brain, or comprises a lymphoma, breast, endometrium, uterus, ovary, testes, lung, prostate, colon, pancreas, esophagus, liver, skin, kidney, adrenal gland and/or brain cancer or tumor, a metastasis or recurring tumor, cancer or neoplasia, a non-small cell lung cancer (NSCLC) and/or head and neck squamous cell cancer (HNSCC).
 71. The method of any one of claims 43 to 70, wherein the method comprises detecting in the subject, the cells or the sample the number or density of Trm cells that are CD19−CD20−CD14−CD56−CD4−CD45+CD3+CD8+ T-cells.
 72. A kit comprising one or more of the modified T-cells according to any one of claims 12 to 40 and/or the composition according to claim 41 and instructions for use.
 73. The kit of claim 72, wherein the instruction for use provide directions to conduct the method of any one of claims 1 to 11 and/or 42 to
 71. 